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The KASH domain protein MSP-300 plays an essential role
The KASH domain protein MSP-300 plays an essential role

... then washed in PBS several times and resuspended in PBS with 1% Triton X-100 for 1 h at room temperature. Blocking was performed by incubating the samples at room temperature in PBST (0.4% Triton X-100 in PBS) with 5% NGS (Normal Goat Serum, Santa Cruz) for at least 1 h. Samples were then incubated ...
Introduction
Introduction

... function of humans, and serves as the foundation of modern medicine. As a discipline, it connects science, medicine, and health, and creates a framework for understanding how the human body adapts to stresses, physical activity, and disease. Human physiology is closely related to anatomy, in that an ...
Listeria In Vivo Dynamic Imaging of the Effector Immune Response to Infection
Listeria In Vivo Dynamic Imaging of the Effector Immune Response to Infection

... has provided high-resolution information on the dynamic interactions that take place during immune responses in situ. Tracking of cells in real time reveals kinetic information that is lacking from static images. Intravital microscopy has been useful in understanding tissue specific responses to pat ...
Human Body Project: You be the Teacher
Human Body Project: You be the Teacher

...  An explanation of how your body system works with other body systems. o More specific instructions will be given to each group depending on the body system assigned.  The name, description and symptoms of diseases that affects your assigned system.  Give a few interesting facts about your assign ...
The Hydra polyp: Nothing but an active stem cell community
The Hydra polyp: Nothing but an active stem cell community

... Cellular biological aspects of stem cells in Hydra The regeneration capacity of the freshwater polyp Hydra is proverbial (Bosch 2007). Polyps can be experimentally dissociated into single cells, then recombined into clumps (‘‘aggregates’’) that will naturally self-organize and form a normal, fully i ...
Recent Progress in Research of the Mechanism of Fertilization in
Recent Progress in Research of the Mechanism of Fertilization in

... scientists, and has thus been the topic of many reviews (Antonie et al. 2001b; Faure and Dumas 2001; Lord and Russell 2002; Raghavan 2003; Weterings and Russell 2004). In angiosperms the embryo sac is embedded deep in the ovary, where the process of double fertilization occurs. The hidden location o ...
Cellartis® iPS Cell to Hepatocyte Differentiation System
Cellartis® iPS Cell to Hepatocyte Differentiation System

... cosmetic, or household item, etc. This product may not be resold or transferred, modified for resale or transfer, or used to manufacture commercial products without written approval from Takara Bio Europe AB. If you require licenses for other use, please contact us by phone at +46 31 758 0900. Your ...
SPIRAL1 Encodes a Plant-Specific Microtubule
SPIRAL1 Encodes a Plant-Specific Microtubule

... created from the P1 sequences and used to locate the SPR1 locus to a 60-kb region. This region was covered by cosmid clones (blue bars), and new CAPS markers were generated from the cosmid sequences. Mapping with these markers finally confined the SPR1 locus to a 37-kb region. Numbers of recombinati ...
Update on the Pathogenesis and Immunotherapy of Esophageal
Update on the Pathogenesis and Immunotherapy of Esophageal

... Overexpression and p53 mutations occurs frequently in both HPV negative and HPV positive ESCC lesions (35). Overexpression of p53 and loss of pRB is considered abnormal (36). RB expression can be even found in high frecuencies in ESCC (higher than p53)(37-39). Immunohistochemically determined loss o ...
Zinc Transporter of Arabidopsis thaliana AtMTP1 is Localized
Zinc Transporter of Arabidopsis thaliana AtMTP1 is Localized

... the endoplasmic reticulum (ER) and other endomembranes (Sze et al. 1999). Plasma membrane H+-ATPase (AHA3) was recovered in fractions 18–21. Bip, an ER lumen marker protein, was also detected in the same fractions as AtMTP1 under the Mg2+-free condition (Fig. 2A). In the presence of Mg2+ (Fig. 2B), ...
Advances in Environmental Biology
Advances in Environmental Biology

... is considered as one of the most important tropical disease by World Health Organization [WHO] [1]. VL causes significant mortality worldwide, constituting an important public health problem [1,2]. Leishmanicidal drugs are costly, high toxic, frequently have unpleasant side effects. In addition, dru ...
ANISOTROPIC EXPANSION OF THE PLANT CELL WALL
ANISOTROPIC EXPANSION OF THE PLANT CELL WALL

... the velocity field was developed by Hejnowicz & Romberger (1984). The approach, called the growth tensor, is based on the strain rate tensor, which Silk & Erickson (1979), using the tools of continuum mechanics, introduced in their treatment of plant growth. A line can move through a growing continuu ...
The Skeletal System
The Skeletal System

... from clients. Biology is the study of life. One of its goals is to discover the unity and the patterns that underlie the diversity of organisms. This reference guide will give people who have an interest in the inner workings of the human body the chance to learn about its functions and structure. T ...
Primordial germ cells migration: morphological and molecular aspects
Primordial germ cells migration: morphological and molecular aspects

... The greatest unsolved question in the field of embryology is exactly which mechanisms maintain the PGCs during their migration as well as which factors control PGC migration and homing within the genital ridges. This process requires integrated signals involving contact of PGCs with extracellular ma ...
THE MAMMALIAN CELL VIRUS RELATIONSHIP (From the
THE MAMMALIAN CELL VIRUS RELATIONSHIP (From the

... and stored at 4°C.; (b) fresh Merck ether repeatedly washed with distilled water and stored at 4°C. (used within a week of opening); and (c) ethylenediamine sodium tetraazetate (versene) for addition to the primary virus suspension in final concentration of 5 X 10-a M to prevent inactivation of nucl ...
The Respiratory System
The Respiratory System

... How Breathing is Controlled • Breathing is not completely voluntary or involuntary • You can change your breathing rate almost any time you want, but you do not have to think about it. Your brain will make sure that you keep breathing. • There are cells in a part of the brain called the medulla obl ...
Canadian Heart Rhythm Society Standing Committee on Device
Canadian Heart Rhythm Society Standing Committee on Device

... center and at least one center that provides follow-up that does not implant devices (Appendix 1). The Chair’s term should be three years, renewable once. The subsequent Chair should be identified in the last year of the Chair’s term, ideally from the Committee membership. 2. Advisories will be dire ...
B. - Genetics
B. - Genetics

... mutation; the effects of mutation on protein structure are minimized by codon redundancy; gene expression and enzyme activity are often subject to homeostatic mechanisms, such as feed-back regulation. Tissue differentiation in complex organisms also depends on stability and variability, in the sense ...
reviews
reviews

... directly juxtaposed to the distally positioned epiblast cells. The VE forms a continuous cell monolayer that overlies both the ExE and the epiblast. Reciprocal signalling between these three cell populations by secreted growth factors of the TGFβ family, including nodal (BOX 1) and bone morphogeneti ...
Chapter 4
Chapter 4

... Microscopes as Windows on the World of Cells – Light microscopes can be used to explore the structures and functions of cells. – When scientists examine a specimen on a microscope slide • Light passes through the specimen • Lenses enlarge, or magnify, the image – Magnification is an increase in the ...
Life Science Leoce review
Life Science Leoce review

... Describe how the cell theory developed and changed over time Collaboration between many other scientists. Scientific theories are based on the best available evidence, but they may change as new evidence is discovered ...
BIOMED 281
BIOMED 281

... 20. The hyperkalemia is caused by all of the following EXCEPT: a) salt substitute b) low renin state c) thiazide diuretic d) ACE inhibitor e) beta-blocker 21. 59-year-old male presents to your office for evaluation of his third calcium oxalate kidney stone. You advise increased fluid intake (2.5-3L ...
lungs - SITH ITB
lungs - SITH ITB

... –  Legs may have helped them lift up to gulp air. –  The fossil fish Tiktaalik •  lived about 375 million years ago and ...
PDF
PDF

... the differences in fate that distinguish one cell from the produce mirror-symmetrical double embryos. In Tub$ex, yolk-poor cytoplasm accumulates at the polar regions other three in the four-cell embryo of the leech, Helob of the precleavage egg and the cell that inherits it gendella triserialis. Dif ...
Mechanisms of cellular communication through intercellular protein
Mechanisms of cellular communication through intercellular protein

... After a long quiescent period, in-depth studies began to understand mechanisms of intercellular protein transfer, which was found to be a widespread event. The importance of such a cellular cross-talk is being well understood now, as new reports of intercellular membrane transfer are published [18, ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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