Stem Cell Basics About this document
... humans: embryonic stem cells and non-embryonic “somatic” or “adult” stem cells. The functions and characteristics of these cells will be explained in this document. Scientists discovered ways to derive embryonic stem cells from early mouse embryos nearly 30 years ago, in 1981. The detailed study of ...
... humans: embryonic stem cells and non-embryonic “somatic” or “adult” stem cells. The functions and characteristics of these cells will be explained in this document. Scientists discovered ways to derive embryonic stem cells from early mouse embryos nearly 30 years ago, in 1981. The detailed study of ...
Activation of β-Catenin in Dendritic Cells Regulates
... Whether β-catenin signaling in DCs is critical for intestinal homeostasis. We compared the frequencies of Treg cells and TH17/TH1 cells in the intestine of β-catDC−/− and β-catfl/fl mice because intestinal DCs and macrophages play an important role in the induction of these subsets. β-catDC−/− mice ...
... Whether β-catenin signaling in DCs is critical for intestinal homeostasis. We compared the frequencies of Treg cells and TH17/TH1 cells in the intestine of β-catDC−/− and β-catfl/fl mice because intestinal DCs and macrophages play an important role in the induction of these subsets. β-catDC−/− mice ...
Hyperproduction of Exocellular Levansucrase by
... Joyeux et al. (1971) showed that cell-bound levansucrase in B. subtilis BS5 may be released from the cells by washing with a high ionic strength buffer. We determined for both induced strains the extent of release of cell-bound enzyme with respect to the KCI concentration of the wash buffer. The dis ...
... Joyeux et al. (1971) showed that cell-bound levansucrase in B. subtilis BS5 may be released from the cells by washing with a high ionic strength buffer. We determined for both induced strains the extent of release of cell-bound enzyme with respect to the KCI concentration of the wash buffer. The dis ...
Corresponding author: Dr. A. Lacey Samuels
... breakdown products were consistently shown to be radioactive, indicating incorporation of 3H-phenylalanine into the lignin polymer (Supplemental Figure 1). To investigate the nature of the soluble metabolites that were labeled during the four hour incubation with 3H-phenylalanine, methanol soluble p ...
... breakdown products were consistently shown to be radioactive, indicating incorporation of 3H-phenylalanine into the lignin polymer (Supplemental Figure 1). To investigate the nature of the soluble metabolites that were labeled during the four hour incubation with 3H-phenylalanine, methanol soluble p ...
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... exploration of biological systems in unprecedented detail. The ability to clone, modify and express a vast array of cellular proteins was extended yet further by the capacity to reconstitute recombinant macromolecules into simple artificial systems. The resulting explosion of knowledge has yielded a ...
... exploration of biological systems in unprecedented detail. The ability to clone, modify and express a vast array of cellular proteins was extended yet further by the capacity to reconstitute recombinant macromolecules into simple artificial systems. The resulting explosion of knowledge has yielded a ...
Transplantation of porcine umbilical cord Matrix cells
... generous gift from P. Hackett) was modified as follows: The plasmid containing the transposon pT/HygR-eGFP (also a gift from Hackett lab) was used as the template to generate a PCR product of the hygromycin resistance-eGFP insert. The neomycin resistance gene from the original transposon vector (pT/ ...
... generous gift from P. Hackett) was modified as follows: The plasmid containing the transposon pT/HygR-eGFP (also a gift from Hackett lab) was used as the template to generate a PCR product of the hygromycin resistance-eGFP insert. The neomycin resistance gene from the original transposon vector (pT/ ...
Earthworm Dissection Pictures: Modern Biology, Holt Purpose: In
... at the first segment, cut the septa (thin membranes) that internally divide the segments, so the skin can be laid flat. Use pins to hold the skin open and expose the organs. Continue to until you have uncovered a centimeter or so of the intestine. 6. Using a scalpel and scissors, make a shallow inci ...
... at the first segment, cut the septa (thin membranes) that internally divide the segments, so the skin can be laid flat. Use pins to hold the skin open and expose the organs. Continue to until you have uncovered a centimeter or so of the intestine. 6. Using a scalpel and scissors, make a shallow inci ...
Safety in clinical lab - الجامعة الإسلامية بغزة
... health. He is anxious about his physical condition; he fear because he does not know what will happen next; his disease may or may not be life threatening; and he is physically uncomfortable as a result of his disease or injury. He is also separated from his family. For these reasons it is important ...
... health. He is anxious about his physical condition; he fear because he does not know what will happen next; his disease may or may not be life threatening; and he is physically uncomfortable as a result of his disease or injury. He is also separated from his family. For these reasons it is important ...
Hematopoietic stem cell—A stem cell that
... IV. What are adult stem cells? An adult stem cell is thought to be an undifferentiated cell, found among differentiated cells in a tissue or organ that can renew itself and can differentiate to yield some or all of the major specialized cell types of the tissue or organ. The primary roles of adult ...
... IV. What are adult stem cells? An adult stem cell is thought to be an undifferentiated cell, found among differentiated cells in a tissue or organ that can renew itself and can differentiate to yield some or all of the major specialized cell types of the tissue or organ. The primary roles of adult ...
Interleukin-6 Gene Expression in Multiple Myeloma: A Characteristic
... Primers for the P-actin gene were used as control for cDNA integrity, for the presence of cells in single-cell PCR experiments, and for PCR. IL-6 mRNA was detected in purified myeloma cells from 10 of 22 patients (45%). IL-6-R mRNA was detected in the 10 patients in whom IL-6 gene expression was fou ...
... Primers for the P-actin gene were used as control for cDNA integrity, for the presence of cells in single-cell PCR experiments, and for PCR. IL-6 mRNA was detected in purified myeloma cells from 10 of 22 patients (45%). IL-6-R mRNA was detected in the 10 patients in whom IL-6 gene expression was fou ...
TGFβ/activin/nodal signaling is necessary for the maintenance of
... can be induced to differentiate to functional cell types of all three primary germ layers in vitro, and upon integration into host animals, they have the capacity to contribute to all cell types of the embryo, including the germline (Smith, 2001). These qualities have made ES cells valuable resource ...
... can be induced to differentiate to functional cell types of all three primary germ layers in vitro, and upon integration into host animals, they have the capacity to contribute to all cell types of the embryo, including the germline (Smith, 2001). These qualities have made ES cells valuable resource ...
mycoplasmas in tissue culture
... no chemically defined medium has yet been devised even for the least exacting members of this group. Present knowledge is based on the results obtained with partially defined medium and with only a few strains of mycoplasma. Nevertheless, it is clear that there is wide variation in the nutritional r ...
... no chemically defined medium has yet been devised even for the least exacting members of this group. Present knowledge is based on the results obtained with partially defined medium and with only a few strains of mycoplasma. Nevertheless, it is clear that there is wide variation in the nutritional r ...
UBXD4, a UBX-Containing Protein, Regulates the Cell Surface
... against GenBank (NIH, genetic sequence database) to identify putative ␣3-interacting proteins. The sequencing of a positive isolated clone was identified as an 589 nt reading frame encoding the N terminus of UBXD4 (clone 42). To verify interactions, clone 42 as well as full-length UBXD4 (see plasmid ...
... against GenBank (NIH, genetic sequence database) to identify putative ␣3-interacting proteins. The sequencing of a positive isolated clone was identified as an 589 nt reading frame encoding the N terminus of UBXD4 (clone 42). To verify interactions, clone 42 as well as full-length UBXD4 (see plasmid ...
Epidermal club cells do not protect fathead minnows against
... provided several lines of empirical evidence in support of the anti-parasite hypothesis. Ostariophysan fish are frequently infected with the resting stage of aquatic trematodes, known as metacercariae. These encyst on and in a range of species-specific tissues, typically awaiting ingestion by an app ...
... provided several lines of empirical evidence in support of the anti-parasite hypothesis. Ostariophysan fish are frequently infected with the resting stage of aquatic trematodes, known as metacercariae. These encyst on and in a range of species-specific tissues, typically awaiting ingestion by an app ...
A3 - Viktor`s Notes for the Neurosurgery Resident
... receptor potentials (produced by sensory stimulus on receptor), synaptic potentials (produced by neurotransmitter on postsynaptic membrane) do not propagate (because these membranes do not have necessary voltage-dependent channels) – these electrotonic potentials must spread passively to patch of ...
... receptor potentials (produced by sensory stimulus on receptor), synaptic potentials (produced by neurotransmitter on postsynaptic membrane) do not propagate (because these membranes do not have necessary voltage-dependent channels) – these electrotonic potentials must spread passively to patch of ...
Splenic Epstein-Barr Virus–Associated Inflammatory Pseudotumor
... differences in the infected cell types.1 Among EBVassociated splenic and hepatic IPTs it is the spindle cell component that is infected, whereas the virus preferentially affects the lymphocytes of EBV-associated nodal cases. The percentage of splenic and hepatic IPTs that are EBV positive is not wel ...
... differences in the infected cell types.1 Among EBVassociated splenic and hepatic IPTs it is the spindle cell component that is infected, whereas the virus preferentially affects the lymphocytes of EBV-associated nodal cases. The percentage of splenic and hepatic IPTs that are EBV positive is not wel ...
Development of the musculoskeletal system: meeting
... conceptual advances have arisen from these studies. Most importantly, the talks revealed that interactions between the different components are crucial for musculoskeletal morphogenesis (Fig. 1). Another emerging theme was the importance of muscle mechanical forces in shaping cartilage and bone duri ...
... conceptual advances have arisen from these studies. Most importantly, the talks revealed that interactions between the different components are crucial for musculoskeletal morphogenesis (Fig. 1). Another emerging theme was the importance of muscle mechanical forces in shaping cartilage and bone duri ...
New metal complexes as potential therapeutics
... fields [2–6]. Some selected compounds that are currently used in clinical diagnosis and treatment are shown in Figure 1. Designing ligands that will interact with free or protein-bound metal ions is also a recent focus of medicinal inorganic research [7–9]. For example, chelating ligands for copper ...
... fields [2–6]. Some selected compounds that are currently used in clinical diagnosis and treatment are shown in Figure 1. Designing ligands that will interact with free or protein-bound metal ions is also a recent focus of medicinal inorganic research [7–9]. For example, chelating ligands for copper ...
Nuclear Envelope Breakdown Proceeds by
... stable protein network (Daigle et al., 2001). In dividing cells, this network is abolished during NE breakdown (NEBD), which defines the transition from prophase to prometaphase in the cell division cycle (Alberts, 1994). The mechanism of NEBD has not been established. Based on indirect evidence, tw ...
... stable protein network (Daigle et al., 2001). In dividing cells, this network is abolished during NE breakdown (NEBD), which defines the transition from prophase to prometaphase in the cell division cycle (Alberts, 1994). The mechanism of NEBD has not been established. Based on indirect evidence, tw ...
Recruiting Coreceptors to the T Cell Receptor Complex
... independent of coreceptors, and that this is followed by recruitment of coreceptors to the triggered TCR-pMHC complex by interactions of the SH2 domain of coreceptor-associated Lck with pTyr in the TCR-CD3 complex. However, Xu and Littman’s coreceptor recruitment model has been largely overlooked by ...
... independent of coreceptors, and that this is followed by recruitment of coreceptors to the triggered TCR-pMHC complex by interactions of the SH2 domain of coreceptor-associated Lck with pTyr in the TCR-CD3 complex. However, Xu and Littman’s coreceptor recruitment model has been largely overlooked by ...
Membrane voltage initiates Ca2 waves and potentiates Ca2
... hormonal physiology, including that of abscisic acid (ABA), which signals conditions of water stress and alters ion channel activities in guard cells of higher-plant leaves. Such changes in [Ca21]i are thought to encode for cellular responses to different stimuli, but their origins and functions are ...
... hormonal physiology, including that of abscisic acid (ABA), which signals conditions of water stress and alters ion channel activities in guard cells of higher-plant leaves. Such changes in [Ca21]i are thought to encode for cellular responses to different stimuli, but their origins and functions are ...
Potentiometric titrations of Bacillus subtilis cells to low pH and a
... surface, and 2) to enable quantitative geochemical modeling of mass transport in bacteria-bearing systems. The lack of consensus for a protonation model has hampered efforts to move forward on both of these fronts. Spectroscopic investigations of metal binding on bacterial cell walls provide some co ...
... surface, and 2) to enable quantitative geochemical modeling of mass transport in bacteria-bearing systems. The lack of consensus for a protonation model has hampered efforts to move forward on both of these fronts. Spectroscopic investigations of metal binding on bacterial cell walls provide some co ...
The fruit fly (Drosophila melanogaster) provides a model for the
... Evolutionary Neuroethology in Jena is not “wasting” his time working on butterflies, but on even less impressive flies with a penchant for rotten fruit. These pests, which can be found in every fruit bowl in summer, are commonly known as either fruit flies or vinegar flies. The fly, sometimes also r ...
... Evolutionary Neuroethology in Jena is not “wasting” his time working on butterflies, but on even less impressive flies with a penchant for rotten fruit. These pests, which can be found in every fruit bowl in summer, are commonly known as either fruit flies or vinegar flies. The fly, sometimes also r ...
LMA® MAD NAsAL - Capes Medical
... Rapidly Effective •A tomized nasal medications absorb into blood stream, avoiding first-pass metabolism •A tomized nasal medications offer rapid absorption across mucosal membranes to the blood stream into the brain and cerebrospinal fluid via olfactory mucosa to nose-brain pathway • Approaches ...
... Rapidly Effective •A tomized nasal medications absorb into blood stream, avoiding first-pass metabolism •A tomized nasal medications offer rapid absorption across mucosal membranes to the blood stream into the brain and cerebrospinal fluid via olfactory mucosa to nose-brain pathway • Approaches ...