The Polypeptide Composition of Moving and Stationary
... they spend most of their time not moving. Using correlative light and electron microscopy, we have shown that these proteins move in the form of assembled neurofilament polymers. However, the polypeptide composition of these moving polymers is not known. To address this, we visualized neurofilaments i ...
... they spend most of their time not moving. Using correlative light and electron microscopy, we have shown that these proteins move in the form of assembled neurofilament polymers. However, the polypeptide composition of these moving polymers is not known. To address this, we visualized neurofilaments i ...
Recruiting Coreceptors to the T Cell Receptor Complex
... sequence of TCR and coreceptor interactions with pMHC in their native environment at the T cell-target cell interface. In a report in this issue of Immunity, Jiang et al. (2011) use a technique developed in their laboratory for this purpose to show that CD8 stabilizes the TCR-pMHC interaction after ...
... sequence of TCR and coreceptor interactions with pMHC in their native environment at the T cell-target cell interface. In a report in this issue of Immunity, Jiang et al. (2011) use a technique developed in their laboratory for this purpose to show that CD8 stabilizes the TCR-pMHC interaction after ...
Moss Systems Biology en Route
... A coordinated progression through the cell cycle in response to environmental signals is of crucial importance for growth and development of eukaryotic cells. The G1/S transition is an essential checkpoint for developmental decisions within the cell cycle and is controlled by the retinoblastoma (Rb) ...
... A coordinated progression through the cell cycle in response to environmental signals is of crucial importance for growth and development of eukaryotic cells. The G1/S transition is an essential checkpoint for developmental decisions within the cell cycle and is controlled by the retinoblastoma (Rb) ...
Levels of Ycg1 Limit Condensin Function during the Cell Cycle
... comprised of five protein subunits: two core ATPase subunits (Smc2 and Smc4), a kleisin subunit (CAP-H/Brn1), and two HEAT-repeat subunits (CAP-G/Ycg1 and CAP-D2/Ycs4), each of which is essential for complex function and cell viability [5–8]. Mammalian cells have two condensin complexes, condensin I ...
... comprised of five protein subunits: two core ATPase subunits (Smc2 and Smc4), a kleisin subunit (CAP-H/Brn1), and two HEAT-repeat subunits (CAP-G/Ycg1 and CAP-D2/Ycs4), each of which is essential for complex function and cell viability [5–8]. Mammalian cells have two condensin complexes, condensin I ...
Stored Red Blood Cell Transfusion Induces Regulatory T Cells
... of CD4⫹CD25⫺ T-responder cells was assessed. After exposure to Ctl or one of the PRBC supernatants (42-day LR) with or without anti-CD3 stimulation for 5 days, CD4⫹CD25⫹ Tregs and CD4⫹CD25⫺ T-responder cells were sorted by magnetic bead immunosorting. Tregs were sorted based on their CD25 expression ...
... of CD4⫹CD25⫺ T-responder cells was assessed. After exposure to Ctl or one of the PRBC supernatants (42-day LR) with or without anti-CD3 stimulation for 5 days, CD4⫹CD25⫹ Tregs and CD4⫹CD25⫺ T-responder cells were sorted by magnetic bead immunosorting. Tregs were sorted based on their CD25 expression ...
immunohistological study of mannan polysaccharides in poplar stem
... Mannan polysaccharides serve as storage reserves in seeds and as structure elements in cell walls, but they may also perform other important functions during plant growth. As one of the major hemicelluloses in angiosperm wood, little is known about the presence and localization of mannan polysacchar ...
... Mannan polysaccharides serve as storage reserves in seeds and as structure elements in cell walls, but they may also perform other important functions during plant growth. As one of the major hemicelluloses in angiosperm wood, little is known about the presence and localization of mannan polysacchar ...
- courses
... From Lower to Higher Level Evolutionary Units. The first common feature is the transition from independent replicators to form higher level units: for example, genes ganged up in protocells, prokaryotes joined to constitute the eukaryotic cell, protist cells stacked together to form multicellular or ...
... From Lower to Higher Level Evolutionary Units. The first common feature is the transition from independent replicators to form higher level units: for example, genes ganged up in protocells, prokaryotes joined to constitute the eukaryotic cell, protist cells stacked together to form multicellular or ...
Targeting of Salmonella typhimurium to Vesicles Containing
... trafficking routes used by intracellular pathogens, S. typhimurium appears to be the only pathogen capable of triggering within the host cell an extensive redistribution of lgps, which results in the formation of filaments containing these proteins which are connected to the S. typhimurium-containin ...
... trafficking routes used by intracellular pathogens, S. typhimurium appears to be the only pathogen capable of triggering within the host cell an extensive redistribution of lgps, which results in the formation of filaments containing these proteins which are connected to the S. typhimurium-containin ...
Fatty acids as gatekeepers of immune cell regulation - Direct-MS
... mRNA [29] and it has been suggested that specific lipid microdomains destined to form caveolae originate in the Golgi apparatus and are transported to the plasma membrane as vesicular organelles [27]. Thus, it would appear that the presence of caveolae at the cell surface might be a transient phenom ...
... mRNA [29] and it has been suggested that specific lipid microdomains destined to form caveolae originate in the Golgi apparatus and are transported to the plasma membrane as vesicular organelles [27]. Thus, it would appear that the presence of caveolae at the cell surface might be a transient phenom ...
Progression Liver Modulates Severe Malaria Disease in the
... (Sigma-Aldrich) and sacrificed 2 h later to assess vascular leakage; brains were isolated in formamide and incubated for 48 h at 37˚C. Absorbance was measured at 620 nm in an ELISA reader with Evans blue concentration assessed via standard curve starting at 400 mg/ml and expressed as milligram dye p ...
... (Sigma-Aldrich) and sacrificed 2 h later to assess vascular leakage; brains were isolated in formamide and incubated for 48 h at 37˚C. Absorbance was measured at 620 nm in an ELISA reader with Evans blue concentration assessed via standard curve starting at 400 mg/ml and expressed as milligram dye p ...
The origin of early primitive streak - Development
... posterior region was more stratified. This gradient in epithelial aspect of the epiblast was used to verify the AP axis in the subsequent studies. Koller’s sickle was also used as a landmark for tagging individual sites of prestreak blastodiscs in ovo. Migration pattern of labeled cells during primi ...
... posterior region was more stratified. This gradient in epithelial aspect of the epiblast was used to verify the AP axis in the subsequent studies. Koller’s sickle was also used as a landmark for tagging individual sites of prestreak blastodiscs in ovo. Migration pattern of labeled cells during primi ...
invited review - AJP
... been previously reached by van den Hove et al. (99) using thyroid hemilobes in culture. Another way to investigate receptor-mediated endocytosis vs. nonspecific uptake is to determine whether certain forms of Tg (differing from others with respect to their hormone content or type of glycosylation) a ...
... been previously reached by van den Hove et al. (99) using thyroid hemilobes in culture. Another way to investigate receptor-mediated endocytosis vs. nonspecific uptake is to determine whether certain forms of Tg (differing from others with respect to their hormone content or type of glycosylation) a ...
Rat maf related genes: specific expression in chondrocytes
... Figure 3 Maf expression in chondrocytes. (a and b): The speci®c antibodies of Maf-1 and Maf-2 were prepared as follows: Fragments of Maf-1 and Maf-2 proteins were produced in E coli as fusion proteins with maltose binding protein and glutathione Stransferase, respectively. maf-1 cDNA fragment corres ...
... Figure 3 Maf expression in chondrocytes. (a and b): The speci®c antibodies of Maf-1 and Maf-2 were prepared as follows: Fragments of Maf-1 and Maf-2 proteins were produced in E coli as fusion proteins with maltose binding protein and glutathione Stransferase, respectively. maf-1 cDNA fragment corres ...
Transcriptionally repressed germ cells lack a subpopulation of
... H5, and with an antibody specific for the germ-line factor PIEWe conclude that the phosphoepitope on RNAP II that is rec1 (Fig. 4). As reported previously (Mello et al., 1996), PIE-1 ognized by mAb H5 is present in somatic nuclei, but not in was detected in germ-line blastomeres from the 1-cell stag ...
... H5, and with an antibody specific for the germ-line factor PIEWe conclude that the phosphoepitope on RNAP II that is rec1 (Fig. 4). As reported previously (Mello et al., 1996), PIE-1 ognized by mAb H5 is present in somatic nuclei, but not in was detected in germ-line blastomeres from the 1-cell stag ...
The role of structural disorder in cell cycle regulation, related clinical
... An important functional property of the IDPs is their large surface available for interaction due to which they are ideal docking platforms for the assembly of large complexes [38]. During the progression of the cell cycle, many such complexes form. One example of these assemblers is a component of ...
... An important functional property of the IDPs is their large surface available for interaction due to which they are ideal docking platforms for the assembly of large complexes [38]. During the progression of the cell cycle, many such complexes form. One example of these assemblers is a component of ...
arc6, an extreme chloroplast division mutant of Arabidopsis also
... orientated parallel with the long axis of the plastid. Chloroplast profiles contain an average of six to ten stacks of three or four appressed membranes. This is in marked contrast to wild-type plastids in which the thylakoid membranes are much less well developed with little obvious organisation (F ...
... orientated parallel with the long axis of the plastid. Chloroplast profiles contain an average of six to ten stacks of three or four appressed membranes. This is in marked contrast to wild-type plastids in which the thylakoid membranes are much less well developed with little obvious organisation (F ...
Nuclear Factor-90 of Activated T-Cells: A Double
... T-cells (NFATs),1 appear to be essential for early T-cell gene activation (1-6). The transcriptional activation of interleukin 2 (IL-2), the first lymphokine secreted following T-cell activation (7), likely involves NFATs as well as other transcription factors including AP-1, NF-κB, and Oct-1 (8, 9) ...
... T-cells (NFATs),1 appear to be essential for early T-cell gene activation (1-6). The transcriptional activation of interleukin 2 (IL-2), the first lymphokine secreted following T-cell activation (7), likely involves NFATs as well as other transcription factors including AP-1, NF-κB, and Oct-1 (8, 9) ...
Compounds from Wild Mushrooms with Antitumor
... themselves from hordes of attacking microbes by developing natural protective substances. Modern scientific studies on the above called “medicinal mushrooms” have expanded exponentially during the last two decades and scientific explanation to show how compounds derived from mushrooms function in hu ...
... themselves from hordes of attacking microbes by developing natural protective substances. Modern scientific studies on the above called “medicinal mushrooms” have expanded exponentially during the last two decades and scientific explanation to show how compounds derived from mushrooms function in hu ...
Nitrogen deficiency inhibits leaf blade growth in Lolium
... Lolium perenne plants grew at low (1 mm) or high (7.5 mm) nitrogen supply. Growth at low nitrogen supply reduced the nitrogen concentration in the leaf growth zone by 40% (P < 0.001; Table 1) and the LER by 43% (P < 0.001;Table 1 & Fig. 1). The latter was because of changes of similar magnitude in b ...
... Lolium perenne plants grew at low (1 mm) or high (7.5 mm) nitrogen supply. Growth at low nitrogen supply reduced the nitrogen concentration in the leaf growth zone by 40% (P < 0.001; Table 1) and the LER by 43% (P < 0.001;Table 1 & Fig. 1). The latter was because of changes of similar magnitude in b ...
The Muir-Torre syndrome - UCSF Pathology
... symptoms and signs. Many syndromes are named after the physicians credited with first reporting the association; these are ʺeponymousʺ syndromes (see also the list of eponymous diseases, many of which are called ʺsyndromesʺ). Otherwise, disease features or presumed causes, as well as references ...
... symptoms and signs. Many syndromes are named after the physicians credited with first reporting the association; these are ʺeponymousʺ syndromes (see also the list of eponymous diseases, many of which are called ʺsyndromesʺ). Otherwise, disease features or presumed causes, as well as references ...