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GelDoc-It Imaging System Using GelRed and GelGreen
GelDoc-It Imaging System Using GelRed and GelGreen

Motilities Swimming, Swarming, and Twitching Adherent Phenotypic
Motilities Swimming, Swarming, and Twitching Adherent Phenotypic

... Pseudomonas aeruginosa is a ubiquitous environmental bacterium capable of forming biofilms on surfaces as a survival strategy. It exhibits a large variety of competition/virulence factors, such as three types of motilities: flagellum-mediated swimming, flagellum-mediated swarming, and type IV pilus- ...
CML cells actively evade host immune surveillance
CML cells actively evade host immune surveillance

Adenomatous polyposis coli - Journal of Cell Science
Adenomatous polyposis coli - Journal of Cell Science

... APC has been shown to regulate cell polarity and migration through control of the actin cytoskeleton (Akiyama and Kawasaki, 2006). Forced expression of APC in the small intestine induces disordered migration of epithelial cells (Wong et al., 1996). By contrast, loss of APC slows down their migration ...
High frequency precise multiple-base reversion of
High frequency precise multiple-base reversion of

... various ratios of revertant viruses C3/R6 (one-base reversion) and C3/R9 (five-base reversions) and mixed infections with wild-type poliovirus and a virus that contained the four silent changes showed that they all have approximately equal growth rates and that they compete rather equally. Both the ...
Inhibition of c-myc Expression in Cells by Targeting an RNA
Inhibition of c-myc Expression in Cells by Targeting an RNA

... in the cell culture medium and ranged from 10 to 750 nM. Details are provided in the figures and figure legends. MTT Assay for Cell Growth. Cell number was assessed with a colorimetric assay based on reduction of the dye 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT; Sigma Chemic ...
Production of fibronectin and collagen types I and III by chick embryo
Production of fibronectin and collagen types I and III by chick embryo

... 51 very low amounts of type III collagen could be detected (5 to 10 ng/ml), even after 7 days of culture; so that it was impossible to assess whether this production was or was not influenced by the substrate. ...
siRNA back - Santa Cruz Biotechnology
siRNA back - Santa Cruz Biotechnology

The viability assessment of Microcystis aeruginosa cells after co-culturing
The viability assessment of Microcystis aeruginosa cells after co-culturing

... of humans, wild and domestic animals and microalgae and conventional water treatment processes are unable to remove the microcystins in the drinking waters (Sigee et al., 1999; de Figueiredo et al., 2004; Oberholster et al., 2009). Thus the control and management of HABs is important and may involve ...
Interaction between Cell Wall and Plasma Membrane via RGD Motif
Interaction between Cell Wall and Plasma Membrane via RGD Motif

... mechanism of signal transmission from the cell wall to plasma membrane during defense responses has been obscure for a long time. The results of the present study are summarized as follows: (1) VN-like and VNR-like proteins were found to exist in the pea cell wall and plasma membrane, respectively, ...
life in a drop of water
life in a drop of water

... a membrane. It is explained that the nucleus is the control center of the cell, that cytoplasm is a fluid in which many cell reactions occur, and that the cell membrane controls what goes into and comes out of a cell. ...
Micronuclei Bearing Acentric Extrachromosomal Chromatin Are
Micronuclei Bearing Acentric Extrachromosomal Chromatin Are

... staining region. DMs consist of multiple, paired, minute chromatins of varying sizes and can be detected when the chromosomes are spread out. DMs generally lack the centromere and are composed of circular DNA that does not require a telomeric structure (for a recent review, see refs. 1, 2). We and o ...
Plastid division
Plastid division

... Plastids undergo a process of binary fission in order to replicate. Plastid replication is required at two distinct stages of plant growth: during cell division to ensure correct plastid segregation, and during cell expansion and development to generate large populations of functional plastids, as i ...
Amino acid sequence identity between the HA1 of influenza A
Amino acid sequence identity between the HA1 of influenza A

... TPCK trypsin in these mammalian cell cultures. Primary CK cells were prepared from newly hatched chicks as described (Maassab et al., 1985). Cells were cultured in DMEM containing D-valine to inhibit " fibroblast cell growth and 10% heat-inactivated foetal calf serum, ~ penicillin, streptomycin and ...
The Membrane Skeleton of a Unicellular Consists
The Membrane Skeleton of a Unicellular Consists

... the skeleton and two other major proteins of 68 and 39 kD are associated with the plasma membrane fraction. None of these components appears to be the same as the major polypeptides (spectrins, band 3) of the erythrocyte ghost, the other cell system in which a well-defined peripheral membrane skelet ...
SNAREs: Cogs and Coordinators in Signaling
SNAREs: Cogs and Coordinators in Signaling

... mutants has now yielded some functional information about these proteins. SYP21 is found at the PVC (da Silva Conceicao et al., 1997) and most likely functions in the trafficking of lytic cargo, as overexpression leads to mistargeting of fusion proteins containing a lytic vacuole sorting signal (For ...
Role of the ABC Transporter Ste6 in Cell Fusion during Yeast
Role of the ABC Transporter Ste6 in Cell Fusion during Yeast

... Strains with abnormal morphology in the absence of a-factor or that failed to undergo the usual morphological changes in response to a-factor or that failed to agglutinate were eliminated. Finally, mating mixtures of mutant strains mixed with a wild-type M A T a strain (FC139) were observed microsco ...
A novel meiosisspecific protein of fission yeast, Meu13p, promotes
A novel meiosisspecific protein of fission yeast, Meu13p, promotes

... and Hawley, 2000), although supportive evidence of this notion is lacking to date. In S.pombe, meiosis is easily induced by simply removing the nitrogen source from the culture medium, whereupon h+/± diploid cells enter directly into meiosis (azygotic meiosis) and haploid cells of the opposite matin ...
Ccbe1 regulates Vegfc-mediated induction of Vegfr3
Ccbe1 regulates Vegfc-mediated induction of Vegfr3

... lymphatic precursor sprouting from the cardinal vein (Fig. 1A-D; Fig. 2A-H; supplementary material Fig. S1E-K). At 5 days postfertilization (dpf), the hu5055, hu6124, hu5142 and hu6410 mutants have a grossly normal, functional blood vasculature, but the lymphatic vasculature is absent (Fig. 1B,D). U ...
Neural bHLH Genes Control the Neuronal versus Glial Fate
Neural bHLH Genes Control the Neuronal versus Glial Fate

... cortex of Ngn2; Mash1 double-mutant mice and suggests that this cell population may prematurely differentiate along the astrocytic pathway, an event that normally does not occur until postnatal stages (Schmechel and Rakic, 1979; Voigt, 1989). To further examine astrocyte differentiation, we analyzed ...
View/Open - Queen Mary University of London
View/Open - Queen Mary University of London

... goblet cells in Barrett’s oesophagus. These results provide fundamental insight into the histogenetic pathways of progression to oesophageal adenocarcinoma in Barrett’s oesophagus. Our data do not mandate inclusion of patients with columnar metaplasia of the distal oesophagus without intestinal meta ...
Integration of the olfactory code across dendritic
Integration of the olfactory code across dendritic

... median. Claws are arranged by the order in which they were imaged; odors are ordered for each cell based on the sorted response of the first imaged claw. (b) Pairwise correlations of claw response profiles (n = 34 Kenyon cells). Correlations of claws from the same Kenyon cell are demarcated in a box ...
Parvalbumin-Expressing Inhibitory Interneurons in Auditory Cortex
Parvalbumin-Expressing Inhibitory Interneurons in Auditory Cortex

... 20 – 80 dB in 15 dB steps, ⱕ10 repetitions). We used this first curve for an initial estimate of CF and sound level threshold. We then obtained a more accurate estimate of CF using a custom tone array (typically 6 frequencies/octave, intensities in 10 dB steps, ⱖ20 repetitions) centered on our initi ...
New Insights on Plant Cell Elongation: A Role for Acetylcholine
New Insights on Plant Cell Elongation: A Role for Acetylcholine

... but they may somehow provide a signalling function also in plants. Supporting this role, receptors have been found [24,25]; among them the glutamate receptors are the better characterised (for review [26]). Serotonin and melatonin [21–23], as well as L-glutamate [27], appear to regulate the root sys ...
EFFECT OF ASCORBIC ACID CONCENTRATION ON
EFFECT OF ASCORBIC ACID CONCENTRATION ON

... with ascorbic acid, numbers of dividing cells were observed in roots quiescent center treated with ascorbic acid. On the other words, ascorbic acid stimulates cells of this region and induces cell division. These results are in agreement with those obtained by Liso et al. [1984] and Kerk and Feldman ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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