Structural Variants in the Human Genome
... genomes and they are a major cause of phenotypic variation • They are common in certain diseases, particularly cancers and behavioral diseases • They are now also showing up in rare diseases and common behavioral diseases such as autism, schizophrenia and many neurological disorders Courtesy of Mike ...
... genomes and they are a major cause of phenotypic variation • They are common in certain diseases, particularly cancers and behavioral diseases • They are now also showing up in rare diseases and common behavioral diseases such as autism, schizophrenia and many neurological disorders Courtesy of Mike ...
Physical Mapping of a 670-kb Region of Chromosomes XVI and XVII
... blocks containing whole chromosomal T. cruzi DNA were incubated with increasing amounts of exonuclease Bal31 following digestion with SfiI restriction enzyme (Fig. 3). The loci h49 were located in two SfiI fragments of 350 kb and 450 kb that were sensitive to the treatment with Bal31. On the other h ...
... blocks containing whole chromosomal T. cruzi DNA were incubated with increasing amounts of exonuclease Bal31 following digestion with SfiI restriction enzyme (Fig. 3). The loci h49 were located in two SfiI fragments of 350 kb and 450 kb that were sensitive to the treatment with Bal31. On the other h ...
Genome Rearrangements Caused by Depletion of Essential DNA
... cycle following gene-product depletion by promoter shut off (Yu et al. 2006). Spontaneous DNA damage was measured by the relocalization of the DNA damage checkpoint protein Ddc2 from a diffuse nuclear pattern to discrete subnuclear foci (Figure 1A) (Melo et al. 2001; Lisby et al. 2004). Following gr ...
... cycle following gene-product depletion by promoter shut off (Yu et al. 2006). Spontaneous DNA damage was measured by the relocalization of the DNA damage checkpoint protein Ddc2 from a diffuse nuclear pattern to discrete subnuclear foci (Figure 1A) (Melo et al. 2001; Lisby et al. 2004). Following gr ...
Characteristics, causes and evolutionary consequences of male
... had evolved a reduced mutation rate, am estimates using Z–A, Z–W or A–W comparisons of chicken–turkey divergence do not differ significantly (Axelsson et al. 2004); had Z rate been reduced for other reasons than male-biased mutation, am estimated from Z–A and Z–W data would be lower than when estima ...
... had evolved a reduced mutation rate, am estimates using Z–A, Z–W or A–W comparisons of chicken–turkey divergence do not differ significantly (Axelsson et al. 2004); had Z rate been reduced for other reasons than male-biased mutation, am estimated from Z–A and Z–W data would be lower than when estima ...
Repair of Site-Specific DNA Double-Strand Breaks in
... loss and gain) of nucleotides. Therefore, we evaluated the efficiency of DSB repair via NHEJ by testing for short deletions (<30 bp; often linked with classical NHEJ) and longer deletions (indicating alternative end joining; Deriano and Roth, 2013) both accompanied by small (#3 bp) insertions at the ...
... loss and gain) of nucleotides. Therefore, we evaluated the efficiency of DSB repair via NHEJ by testing for short deletions (<30 bp; often linked with classical NHEJ) and longer deletions (indicating alternative end joining; Deriano and Roth, 2013) both accompanied by small (#3 bp) insertions at the ...
Functional analysis of plastid DNA replication origins in tobacco by
... also hybridised with a probe derived from Lambda DNA in order to detect size marker bands. (b) Schematic depiction of the relative positions of the probe used in 2(a) and detected fragments. Drawing is not to scale. ...
... also hybridised with a probe derived from Lambda DNA in order to detect size marker bands. (b) Schematic depiction of the relative positions of the probe used in 2(a) and detected fragments. Drawing is not to scale. ...
Genome Evolution Due to Allopolyploidization in Wheat
... et al. 2002), still share a high degree of gene synteny and DNA sequence homology. However, they differ from one another by a number of noncoding and highly repetitious DNA sequences (Flavell 1982), and many functional gene complexes (Wicker et al. 2011 and references therein). In spite of this gene ...
... et al. 2002), still share a high degree of gene synteny and DNA sequence homology. However, they differ from one another by a number of noncoding and highly repetitious DNA sequences (Flavell 1982), and many functional gene complexes (Wicker et al. 2011 and references therein). In spite of this gene ...
Mutation Rates
... Ultimately, the source of genetic variation observed among individuals in populations is gene mutation. Mutation generates new alleles, and these are the substance of all evolutionary change. The mutation rate is defined as the probability that a copy of an allele changes to some other allelic form ...
... Ultimately, the source of genetic variation observed among individuals in populations is gene mutation. Mutation generates new alleles, and these are the substance of all evolutionary change. The mutation rate is defined as the probability that a copy of an allele changes to some other allelic form ...
Integrated genome sequence and linkage map of physic nut
... 812 Pingzhi Wu et al. of the connected contigs was generally correct. Third, we used the LASTZ program to perform whole genome scaffoldto-scaffold alignment of our physic nut genome assembly (632 scaffolds were covered) with that presented by Hirakawa et al. (2012). The two genomes exhibited good c ...
... 812 Pingzhi Wu et al. of the connected contigs was generally correct. Third, we used the LASTZ program to perform whole genome scaffoldto-scaffold alignment of our physic nut genome assembly (632 scaffolds were covered) with that presented by Hirakawa et al. (2012). The two genomes exhibited good c ...
Identification of Short Motifs for Comparing Biological Sequences
... For example, sequences that carry the same restriction enzymes’ cut positions [16] might be related and would have similar functions. It would be the same with sequences that carry transcription factor binding sites; other signals would be motifs of specific nature, unique shortest substrings [17] w ...
... For example, sequences that carry the same restriction enzymes’ cut positions [16] might be related and would have similar functions. It would be the same with sequences that carry transcription factor binding sites; other signals would be motifs of specific nature, unique shortest substrings [17] w ...
Evolution of Gene Order and Chromosome Number in
... legitimate, which depends on whether the recombining sites have local sequence similarity (not genomic location similarity). Illegitimate translocation involves reciprocal recombination between apparently random sites in two chromosomes. Each illegitimate translocation increases the number of duplic ...
... legitimate, which depends on whether the recombining sites have local sequence similarity (not genomic location similarity). Illegitimate translocation involves reciprocal recombination between apparently random sites in two chromosomes. Each illegitimate translocation increases the number of duplic ...
... cerevisiae ⴛ S. kudriavzevii hybrids under study indicates that they originated from a single hybridization event. After hybridization, the hybrid genome underwent extensive chromosomal rearrangements, including chromosome losses and the generation of chimeric chromosomes by the nonreciprocal recomb ...
News from the west: Ancient DNA from a French megalithic burial
... European female lineages. Setting aside the possibility of significant post-Neolithic migrations, they proposed a Paleolithic ancestry for modern Europeans. Nonetheless, two recent publications (Bramanti et al., 2009; Malmström et al., 2009) reported sequences from late European hunter-gatherers tha ...
... European female lineages. Setting aside the possibility of significant post-Neolithic migrations, they proposed a Paleolithic ancestry for modern Europeans. Nonetheless, two recent publications (Bramanti et al., 2009; Malmström et al., 2009) reported sequences from late European hunter-gatherers tha ...
Repeat mediated gene duplication in the Drosophila
... sequences flanking DNA duplications and those found at the breakpoints of chromosomal inversions suggests a connection between these two events. In Drosophila, duplicated genes near inversion breakpoints can arise via unequal genetic exchange during the non-allelic crossing over event giving rise to ...
... sequences flanking DNA duplications and those found at the breakpoints of chromosomal inversions suggests a connection between these two events. In Drosophila, duplicated genes near inversion breakpoints can arise via unequal genetic exchange during the non-allelic crossing over event giving rise to ...
Ubiquitin-Like Protein 5 Positively Regulates Chaperone Gene
... Figure 1.—(A) Hypothesized components of a mitochondrial UPR. Physiological and developmental cues impose an unfolded protein load on the mitochondria. The resultant physiological stress activates the afferent limb of the UPRmt, increasing expression of genes encoding mitochondrial chaperones. The l ...
... Figure 1.—(A) Hypothesized components of a mitochondrial UPR. Physiological and developmental cues impose an unfolded protein load on the mitochondria. The resultant physiological stress activates the afferent limb of the UPRmt, increasing expression of genes encoding mitochondrial chaperones. The l ...
Manipulating Yeast Genome Using Plasmid Vectors. In: Gene Expression Technology.
... we discuss below require that one be able to find the rare cells that have undergone a recombination or loss event to remove the plasmid DNA sequences. Unfortunately, the L YS2 gene is quite large, and contains sites within the coding sequence for many of the most common enzymes used in plasmid cons ...
... we discuss below require that one be able to find the rare cells that have undergone a recombination or loss event to remove the plasmid DNA sequences. Unfortunately, the L YS2 gene is quite large, and contains sites within the coding sequence for many of the most common enzymes used in plasmid cons ...
PDF
... mRNAs can be matched to each assembly. The 10 longest mRNA sequences are all matched well with both assemblies, except for the piccolo (Pico) gene (coding for a presynaptic cytomatrix protein): paradoxically, it is matched in chromosome 12 in Cel2 and in chromosome 5 in MGSCv3. mRNA sequences can on ...
... mRNAs can be matched to each assembly. The 10 longest mRNA sequences are all matched well with both assemblies, except for the piccolo (Pico) gene (coding for a presynaptic cytomatrix protein): paradoxically, it is matched in chromosome 12 in Cel2 and in chromosome 5 in MGSCv3. mRNA sequences can on ...
Nematode genome evolution
... Most nematodes have haploid chromosome numbers of n=4–12 (Walton, 1959). The karyotypes of just ~300 species have been studied, but nematodes display a lot of karyotypic variation (Špakulová and Casanova, 2004). The lowest haploid number is n=1 in Parascaris univalens, but very high counts are seen ...
... Most nematodes have haploid chromosome numbers of n=4–12 (Walton, 1959). The karyotypes of just ~300 species have been studied, but nematodes display a lot of karyotypic variation (Špakulová and Casanova, 2004). The lowest haploid number is n=1 in Parascaris univalens, but very high counts are seen ...
Surveying Saccharomyces Genomes to Identify Functional Elements
... sequences within proteins, but has been less useful for identifying functional sequence elements in nonprotein-coding DNA because the relatively rapid rate of change of nonprotein-coding sequences and the relative simplicity of non-coding regulatory sequence elements necessitates the comparison of s ...
... sequences within proteins, but has been less useful for identifying functional sequence elements in nonprotein-coding DNA because the relatively rapid rate of change of nonprotein-coding sequences and the relative simplicity of non-coding regulatory sequence elements necessitates the comparison of s ...
Enlis Genome Research - Release Notes
... - Variation Filter tool: Added filter for DANN predicted deleterious variations, with the option to select different score levels - Variation Filter tool: Added "Ignore No-call positions" to "Not in single genome" and "Not in genome set" filters. This allows for improved comparisons between genomes ...
... - Variation Filter tool: Added filter for DANN predicted deleterious variations, with the option to select different score levels - Variation Filter tool: Added "Ignore No-call positions" to "Not in single genome" and "Not in genome set" filters. This allows for improved comparisons between genomes ...
Comparative Analysis of Structural Diversity and
... TblastN searches of National Center for Biotechnology Information’s (NCBI) expressed sequence tag (EST) database, using an angiosperm mitochondrial copy of the gene as a query. GenBank numbers for identified ESTs are in supplementary table S1, Supplementary Material online. Mitochondrial targeting o ...
... TblastN searches of National Center for Biotechnology Information’s (NCBI) expressed sequence tag (EST) database, using an angiosperm mitochondrial copy of the gene as a query. GenBank numbers for identified ESTs are in supplementary table S1, Supplementary Material online. Mitochondrial targeting o ...
Application Note: Targeted sequencing and chromosomal haplotype
... allows for sequencing and haplotyping of individual genes, chromosomes and genomes. ...
... allows for sequencing and haplotyping of individual genes, chromosomes and genomes. ...
genomebiology.com
... exons/introns from previously intronic/exonic sequences, respectively), and insertion into first and last exons. However, no extensive analysis has compared the effects of TEs on the transcriptomes of mammals, non-mammalian vertebrates and invertebrates. Results: We analyzed the influence of TEs on ...
... exons/introns from previously intronic/exonic sequences, respectively), and insertion into first and last exons. However, no extensive analysis has compared the effects of TEs on the transcriptomes of mammals, non-mammalian vertebrates and invertebrates. Results: We analyzed the influence of TEs on ...
Biophysics_lecture(17Nov06)
... Amenable to molecular genetic manipulation of the nuclear, chloroplast and mitochondrial genomes ...
... Amenable to molecular genetic manipulation of the nuclear, chloroplast and mitochondrial genomes ...
Mitochondrial Transcript Processing and Restoration of Male Fertility
... that cosegregated with the rf2-m8122 Mutator-induced allele in large testcross families was cloned. This fragment was shown to contain a portion of the rf2 gene via allelic cross-referencing experiments. Allelic cross-referencing is the comparison of new DNA polymorphisms between a progenitor allele ...
... that cosegregated with the rf2-m8122 Mutator-induced allele in large testcross families was cloned. This fragment was shown to contain a portion of the rf2 gene via allelic cross-referencing experiments. Allelic cross-referencing is the comparison of new DNA polymorphisms between a progenitor allele ...