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The Endo-β-1,4-Glucanase of Bacillus amyloliquefaciens Is
The Endo-β-1,4-Glucanase of Bacillus amyloliquefaciens Is

... standard protocols [16]. Restriction enzymes, DNA ligase, and other DNA enzymes were used according to the manufacturers’ recommendations. Cell preparation and transformation of B. amyloliquefaciens TB2 were performed as described by Yasbin et al. [22]. To obtain the eglS overexpression construct, t ...
The constitution and the control of the ovarian
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... arrested at the diplotene stage. One main factor involved in ultimate stage of ovarian follicle formation is FIGLA, a germ cell-specific bHLH transcription factor (Fig. 1). Female mice lacking Figla are sterile due to the absence of follicles and oocytes (Soyal et al., 2000). The defect appears afte ...
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... – The choice of homologue sequences – The multiple alignment method used – The method for converting sequences differences into evolutionary distances – The cluster distance definitions for assessing the similarities between clusters EE550 Week 9 ...
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Slides - Workforce Development in Stem Cell Research

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... Sturtevant used the testcross design to map the relative positions of three fruit fly genes: body color (b), wing size (vg), and eye color (cn). o Cinnabar (cn), one of many Drosophila genes affecting eye color, results in a bright red eye. o The recombination frequency between cn and b is 9%. o The ...
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... Heterozygous mutations in the CXCR4 gene WHIM syndrome is an immunodeficiency disease characterized by neutropenia, hypogammaglobulinemia, and extensive human papillomavirus (HPV) infection [4, 5]. Heterozygous mutations in the ELANE gene are responsible for the majority of cases of SCN [6]. ELANE c ...
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... the mathematics of these phenomena. Emphasizing the mathematical aspect of Mendel’s laws through crossover and recombination will prepare the students to make an early realization that biology, in addition to being experimental, IS a computational science. This can serve as a first step towards a br ...
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... study (GWAS) of Siamese and Birman cats (100 of each, half affected, half controls) and came up with some intriguing findings that warrant further study (which is ongoing). It was the Birman cohort that shed most light on the condition as they were all purebred cats. Cats that owners described as Si ...
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Lecture Presentation to accompany Principles of Life

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In-class Interactive Case Discussion PowerPoint

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chesler_reviewer_res..
chesler_reviewer_res..

... method for epistasis detection is not given. *This section can be removed, or simply retitled to reflect it’s content more accurately as epistasis. We chose a single transcript for this analysis, and did not perform the analysis over the entire dataset. We intended it merely as an illustration of th ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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