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fgdfgdgsty - Latvijas universitāte
fgdfgdgsty - Latvijas universitāte

... control groups isolated in 2009-2010. 81 cultures were isolated from blood (n=45) and intravenous catheters (n=36) in a case of laboratory confirmed bacteraemia from patients of four hospitals in Riga, Latvia. Fifty-five S.epidermidis isolates from normal nose epithelium flora were included in the c ...
Searching for adult stem cells in the intestine
Searching for adult stem cells in the intestine

... (Morita et al, 2004). In our adult heterozygous Lgr5-LacZ mice, we could observe the expression of the transgene in rare, scattered cells in the eye, brain, hair follicle, mammary gland, reproductive organs, stomach and intestinal tract. In the small intestine, Lgr5 expression was confined to the CB ...
Taq DNA Polymerase
Taq DNA Polymerase

... Start the PCR program. Once the thermal cycler has reached 94°C, place the PCR tubes in the thermal cycler. In many cases, this simplified hot start improves the specificity of the PCR. Note: After amplification, samples can be stored overnight at 2–8°C, or at –20°C for longer storage. 7. PCR produc ...
on Mendel`s principles of heredity
on Mendel`s principles of heredity

... From Mendel to DNA • Mendel had not actually seen genes - this was an inference or deduction from his experiments. • He had only observed outward visible traits that seemed to confirm the existence of unknown inner entities (genes). ...
The Biological Performance of Calcium Hydroxide
The Biological Performance of Calcium Hydroxide

... the positive control group were lower than that for the negative control group (P < .01). On day 1, the number of CFUs in the 2 Ca(OH)2 microcapsule groups was higher than the positive control group (P < .01). On day 3, the number of CFUs in the 2 Ca(OH)2 microcapsule groups was lower than those in ...
SYLLABUS. Course 2015-2016 1 Degree
SYLLABUS. Course 2015-2016 1 Degree

... acknowledgments in molecular, cellular, and population genetics to allow the in deep analysis of these matters addressed in later courses. Genetics tools are involved in most biotechnological approaches both, in public research institutions and private companies, making of this subject an essential ...
Marker Saturation and Construction of a High
Marker Saturation and Construction of a High

... and haplotype2 refers to the recessive allele (r). Similarly, the fully susceptible parent ...
physical maps
physical maps

... Genome wide identification of genetic markers ...
lecture05_11
lecture05_11

... • When searching for a motif in a genome using PSSM or other methods – the motif is usually found all over the place ->The motif is considered real if found in the vicinity of a gene. • Checking experimentally for the binding sites of a specific TF (location analysis) – the sites that bind the motif ...
Genetic recombination
Genetic recombination

... Disjunction of homologous chromosomes Migration of BC chromosomes to poles (n chromosomes to each poles) !!! Independent combination of nonhomologous chromosomes = interchromosomal recombination  2n ...
Export To Word
Export To Word

... biology that most high school students fail to conceptualize is the fact that all cells in the animal or human body contain the same DNA, yet different cells in different tissues express, on the one hand, a set of common genes, and on the other, express another set of genes that vary depending on th ...
Urania basin brine Bannock basin interface l`Atalante basin interface
Urania basin brine Bannock basin interface l`Atalante basin interface

... Fragment size (base pairs) ...
Functional Genomics and Abiotic
Functional Genomics and Abiotic

... Drought tolerance is a key trait of interest for cereal breeding. Predictions of humanpopulation growth globally, and accompanying shortages of arable land and water supply emphasize a need for crop-plant development in this area. In Australia, drought stress continues to be a major factor affecting ...
Role of Epigenetics in Stem Cell Proliferation and Differentiation
Role of Epigenetics in Stem Cell Proliferation and Differentiation

... these MSCs can also be used to overexpress nerve growth factor (NGF) to alleviate memory deficits in Alzheimer’s disease (AD) [9]. However, one of the drawbacks of stem cell transplantation is the immune response that arises during allogenic transplantation [10]. However, at least one study has demo ...
PPT1
PPT1

... • Collect all known sequences that bind a certain TF. • Align all sequences (using multiple sequence alignment). • Compute the frequency of each nucleotide in each position (PSPM). • Incorporate background frequency for each nucleotide (PSSM). ...
PopCycle Tutorial
PopCycle Tutorial

... bar graph now shows you how many zygotes of each genotype we should expect. If your Punnett square prediction was not correct, try to figure out why. 7. Shepherd the zygotes from the ideal population around their life cycle—to juveniles with 100 % survival, to adulthood with no migration, and to the ...
Biology
Biology

... 80% of questions will come from study guide and standardized test prep (end of each chapter) ...
S010
S010

... Primary transcripts of microRNAs can produce multiple functional products. For example, around a half of mammalian microRNAs are located in introns of protein-coding genes. In these cases, the microRNA and the protein-coding mRNA are processed from the same transcript. Similarly, around a third of a ...
VI P M
VI P M

... which has been used in a yeast two hybrid screen to identify potential interaction partners. A cDNA was identified encoding a novel forkhead transcription factor, so called AcFKH1, which is the first characterized member of the forkhead gene family in filamentous fungi. We have verified this protein ...
Here`s - MathBench
Here`s - MathBench

... Vogon, and after several beers, they begin to discuss alien physiology. Kirk starts by saying that Spock once told him that occasionally, a Vulcan child would be born without pointy ears, and that that child would also seem to be lacking in the ability to mind-meld. " What an amazing co-incidence!" ...
THE DNA OF CAENORHABDITIS ELEGANS HE small
THE DNA OF CAENORHABDITIS ELEGANS HE small

... D N A extraction. The nematodes are frozen in liquid nitrogen and then ground to powder which is gently stirred into 20 volumes of a solution containing 0.1 M NaCl, 0.1 M EDTA, 0.05 M Tris-HC1 (pH 8.0), 1% SDS and 200 pg/ml of protease (Sigma Type VI). An overnight incubation at 37" is followed by p ...
How did I get this? Prenatal and neonatal screening Ultrasound
How did I get this? Prenatal and neonatal screening Ultrasound

... drugs, infectious diseases during pregnancy, or problems that develop during labour. Such factors cause disorders in which the baby’s genetic information remains unchanged and which are therefore not considered to be genetic disorders. Congenital disorders can also be caused by changes in the geneti ...
Introduction The non-vascular, multicellular land plant
Introduction The non-vascular, multicellular land plant

... transformation of a transgenic Physcomitrella line harboring a plasmid containing a resistance marker gene with a second plasmid containing a different selection marker gene. Co-segregation analysis and molecular analysis confirmed that the second plasmid was integrated at the site of insertion of t ...
Familial Mediterranean Fever (FMF) factsheet for patients
Familial Mediterranean Fever (FMF) factsheet for patients

... about 25,000 different genes, which is more than a chicken but less than a tomato. Each cell contains two copies of each gene, one from each of our parents. Within each cell, the genes are arranged into 46 long strings, called ...
Meiosis/Crossing Over - Peoria Public Schools
Meiosis/Crossing Over - Peoria Public Schools

... Mendel’s law of independent assortment could not account for. Thomas Hunt Morgan developed the notion of linked genes to account for the anomalies. (1.8) ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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