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Microsporogenesis in Maize`
Microsporogenesis in Maize`

... translated after meiosis (4, 8). Similar evidence has been obtained Densitometer and Integrator and the values averaged. To deterfor other enzymes in corn (1, 3, 6, 7), in Clarkia dudleyana (14), mine whether the stain density in the color slides was proporin tomato (13), and in Cucurbita (5). The p ...
Interfacial Behavior of a Hairpin DNA Probe Immobilized on Gold
Interfacial Behavior of a Hairpin DNA Probe Immobilized on Gold

... The hairpin DNA probes were immobilized on the gold surface by forming mixed self-assembled monolayers of thiolated singlestranded oligonucleotides (HS-ssDNA) and a spacer thiol (4mercaptobutan-1-ol, MCB) by spontaneous coadsorption. The DNA probes were mixed with MCB in a 1/1 molar fraction to obta ...
The ThyroSeq V2.1 multi-gene next-generation sequencing panel is
The ThyroSeq V2.1 multi-gene next-generation sequencing panel is

... Thyroid nodules are very common and raise the possibility of thyroid cancer. The general recommendation is that nodules larger than 1 cm should be evaluated with a thyroid biopsy. While a thyroid biopsy gives a diagnosis in most cases, up to 10-20% of biopsies are indeterminate, meaning that a diagn ...
Chromosomes and inheritance
Chromosomes and inheritance

... colorblindness (ONE from each parent). Why is it that the sons could be more prone to colorblindness? He must inherit (receive) only ONE recessive allele. This is due to there being no gene for color vision on the Y chromosome. ...
A locus for posterior polymorphous corneal dystrophy (PPCD3
A locus for posterior polymorphous corneal dystrophy (PPCD3

... corneal dystrophy characterized by thickening of Descemet’s membrane and transformation of corneal endothelial cells into cells with an epithelial-like appearance [Krachamer, 1985]. The clinical phenotype of PPCD can vary from relatively benign Descemet’s thickening to severe progression towards vis ...
A strategy for extracting and analyzing large
A strategy for extracting and analyzing large

... of the double mutant. Indeed, a double mutant could have a more severe phenotype than either single mutant and still represent a synthetic, neutral, or alleviating interaction. Typically, large-scale studies have scored gene-gene interactions in a binary manner (synthetic sick/lethal or noninteracti ...
20060710_GOannotCamp_Stanford
20060710_GOannotCamp_Stanford

...  Get intersection of genes from three approaches and use these  This is not necessarily a complete set but is a good starting point (a starting set of a few hundred genes would be good.)  See how big the resulting gene sets are and go from there.  Single ortholog per human gene, 1:1, best hit on ...
Independent Assortment of Chromosomes
Independent Assortment of Chromosomes

... The fertilized egg is called a zygote and has one set of chromosomes from each parent The zygote produces somatic cells by mitosis and develops into an adult At sexual maturity, the ovaries and testes produce haploid gametes Gametes are the only types of human cells produced by meiosis, rather than ...
Genetics - 4J Blog Server
Genetics - 4J Blog Server

... Scientists have put in order the 3.1 billion units of DMA that make up the human genome. Genes carry various hereditary conditions, ranging from cystic fibrosis to Alzheimer's. Now it's possible to test whether people might have certain genetic disorders. Rapid technological advances in genetics tes ...
In Silico method for identification of MHC class I
In Silico method for identification of MHC class I

... complex,  class  I‐related  MR1”.  All  these  BLAST  pairs  cluster  with  the  human  MR1  gene,  forming  an  interspecies  groups  distinct  from  CD1  (Figure  4,  Table  S2).  Our  MR1  results  show  that  this  in  silico  prediction method can also be used for MHC class I‐like molecules oth ...
Against the Central Dogma
Against the Central Dogma

... qPCR array have validated primer sets for wellannotated human lncRNAs that are registered in the lncRNA database created by Dr. John Mattick. The lncRNA profiling begins by adding the produced lncRNA cDNA, 2xSYBR Green, and the lncRNA primers provided to your favorite qPCR plate. The delta-Ct values ...
Probing Essential Nucleobase Functional Groups in Aptamers and
Probing Essential Nucleobase Functional Groups in Aptamers and

... dNAIM was then applied to study two RNA-ligating deoxyribozymes. First, we analyzed the 7S11 deoxyribozyme,16 which catalyzes the formation of 20 ,50 -branched RNA by forming a phosphodiester bond between the 20 OH group of an internal adenosine in one substrate and the 50 -end of a second RNA subst ...
COP9: A New Genetic Locus lnvolved in Light
COP9: A New Genetic Locus lnvolved in Light

... and a domain homologous to the p subunit of trimeric G-proteins (Deng et al., 1992). This nove1 structure suggests that the COR gene product not only has the potential to directly bind DNA through its Zn binding domain, it may also have the ability to interact with other protein components through i ...
Chapter 13 Overview: Variations on a Theme • Living organisms are
Chapter 13 Overview: Variations on a Theme • Living organisms are

... The fertilized egg is called a zygote and has one set of chromosomes from each parent The zygote produces somatic cells by mitosis and develops into an adult At sexual maturity, the ovaries and testes produce haploid gametes Gametes are the only types of human cells produced by meiosis, rather than ...
Respect For Persons As A Guide To Genetic Enhancement
Respect For Persons As A Guide To Genetic Enhancement

... possible that foreknowledge of your condition will be helpful in determining treatment or prevention? And fmally, what if the condition is untreatable? Tensions arise when we are faced with difficult questions such as these, because previously they did not exist. At some point, it may have been bett ...
Abnormalities - Spring Branch ISD
Abnormalities - Spring Branch ISD

... Recombination of Unlinked Genes: Independent Assortment of Chromosomes • Mendel observed that combinations of traits in some offspring differ from either parent • Offspring with a phenotype matching one of the parental phenotypes are called parental types • Offspring with nonparental phenotypes (ne ...
Hodgkin and Reed-Sternberg–like cells in B-cell
Hodgkin and Reed-Sternberg–like cells in B-cell

... same tissue specimens on the same day as the HRS-like and B-CLL cells and (2) aliquots of the buffer covering the sections obtained during micromanipulation experiments. Genomic DNA of some micromanipulated cells and controls was amplified following the PEP protocol by Zhang et al.11 From aliquots o ...
Paper I- Discussion Points
Paper I- Discussion Points

... much larger distance from the cyan (red) and green dots than in the previous experiment, indicating that the cyan (red) locus replicates a lot earlier (being closer to the origin) than the green locus, which is farther away. And there is no correlation between the mean replication time and the coloc ...
From: colby@bio
From: colby@bio

... donors. Altruists would disappear from a population as the nonaltruists would reap the benefits, but not pay the costs, of altruistic acts. Many behaviors appear altruistic. Biologists, however, can demonstrate that these behaviors are only apparently altruistic. Cooperating with or helping other or ...
Biology
Biology

... seen in protists. 8. Summarize how mass extinctions have affected the evolution of life on Earth. 9. Relate the development of ozone to the adaptation of life to the land. 10. Identify the first multicellular organisms to live on land. 11. Name the first animals to live on land. 12. List the first v ...
Unit 8a-Classical Genetics
Unit 8a-Classical Genetics

... masked by dominant alleles (Mendel crossed a pure PP (purple)  plant with a pure pp (white)  plant, all F1 were Pp (purple))  ...
MOLECULAR PROFILING OF RICE (Oryza sativa L
MOLECULAR PROFILING OF RICE (Oryza sativa L

... in a mapping population it is possible to identify the putative candidate genes associated with the marker sequence. Thus study of quantitative ...
Inactivation of Antibiotics and the Dissemination of
Inactivation of Antibiotics and the Dissemination of

... 6-lactamases. In the years after the introduction of the [3-1actam antibiotics (penicillins and cephalosporins) for the treatment of Gram-negative and Gram-positive inteections, there has been a constant tugof-war between the pharmaceutical industry and the bacterial population: the one to produce a ...
Mendelian Traits
Mendelian Traits

... about the biological nature of the gene. There are many ways in which the DNA blueprint for the PAH enzyme can go awry and if any one of them happens, then the translated product of that DNA will not work correctly. As a result, over 500 different alleles at the PAH locus can cause the disorder (Scri ...
Gene: A part on the chromosomes that holds the information for a
Gene: A part on the chromosomes that holds the information for a

... A part on the chromosomes that holds the  information for a trait.  Remember, you get  one gene from your mother and one from  your father. ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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