
Characterization and expression of an mRNA encoding a wound
... polygalacturonase (PG) (EC 3.1.1.5) (Taylor et ai, 1993; screening strategy. The clone has a high degree of Webb et al., 1993). In tomato, mRNAs encoding these homology at the amino acid level to both the potato enzymes have been characterized from both leaf and win1 and 2 genes, Hevea brasiliensis ...
... polygalacturonase (PG) (EC 3.1.1.5) (Taylor et ai, 1993; screening strategy. The clone has a high degree of Webb et al., 1993). In tomato, mRNAs encoding these homology at the amino acid level to both the potato enzymes have been characterized from both leaf and win1 and 2 genes, Hevea brasiliensis ...
What Are Lethal Whites?
... merle gene, and will produce a pup that is mostly white, and usually deaf and/or blind. This is the "Lethal White" Aussie. Lethal Whites can only result from a merle to merle breeding, but not every puppy in the litter is a homozygous (MM) white. Statistics indicate that approximately 25% of the lit ...
... merle gene, and will produce a pup that is mostly white, and usually deaf and/or blind. This is the "Lethal White" Aussie. Lethal Whites can only result from a merle to merle breeding, but not every puppy in the litter is a homozygous (MM) white. Statistics indicate that approximately 25% of the lit ...
HPV DNA PARTITIONING DURING MITOSIS AS FOLLOWED
... Human papillomaviruses (HPVs) are small, double-stranded deoxyribonucleic acid (DNA) tumor viruses capable of establishing persistent infections in the epithelia. After infecting actively-dividing basal cells, the papillomavirus (PV) genome is maintained as extrachromosomal nuclear plasmids. It is l ...
... Human papillomaviruses (HPVs) are small, double-stranded deoxyribonucleic acid (DNA) tumor viruses capable of establishing persistent infections in the epithelia. After infecting actively-dividing basal cells, the papillomavirus (PV) genome is maintained as extrachromosomal nuclear plasmids. It is l ...
マニュアル Megaprime DNA Labelling System
... 5. Megaprime DNA labelling protocols The Megaprime systems allow DNA from a variety of sources to be labelled in vitro to high specific activity with 32P and other radionuclides. The specific activity of the probes generated by these systems will vary according to the specific activity of the label ...
... 5. Megaprime DNA labelling protocols The Megaprime systems allow DNA from a variety of sources to be labelled in vitro to high specific activity with 32P and other radionuclides. The specific activity of the probes generated by these systems will vary according to the specific activity of the label ...
The Arabidopsis RAD51 paralogs RAD51B, RAD51D and XRCC2
... Homologs of DMC1 and RAD51 have been studied in many eukaryotes, including fungi, invertebrate animals and plants (Bishop et al., 1992; Habu et al., 1996; Klimyuk & Jones, 1997; Couteau et al., 1999). In Arabidopsis thaliana, they function in DNA repair via HR in somatic or meiotic cells (Couteau et ...
... Homologs of DMC1 and RAD51 have been studied in many eukaryotes, including fungi, invertebrate animals and plants (Bishop et al., 1992; Habu et al., 1996; Klimyuk & Jones, 1997; Couteau et al., 1999). In Arabidopsis thaliana, they function in DNA repair via HR in somatic or meiotic cells (Couteau et ...
6 Meiosis and Mendel - Speedway High School
... Traits are characteristics* that are inherited, such as eye color, leaf shape, or tail length. Scientists recognized that traits are hereditary, or passed from one generation to the next, long before they understood how traits are passed on. Genetics is the study of biological inheritance patterns a ...
... Traits are characteristics* that are inherited, such as eye color, leaf shape, or tail length. Scientists recognized that traits are hereditary, or passed from one generation to the next, long before they understood how traits are passed on. Genetics is the study of biological inheritance patterns a ...
ppt - Chair of Computational Biology
... outline of one superimposed on the other. The transition involves repositioning of the two loci within the three-dimensional space of the nucleus. In this hypothetical example, the bottom configuration in each case is favored on transcriptional activation of the loci. Two alternative models have bee ...
... outline of one superimposed on the other. The transition involves repositioning of the two loci within the three-dimensional space of the nucleus. In this hypothetical example, the bottom configuration in each case is favored on transcriptional activation of the loci. Two alternative models have bee ...
Specific oligonucleotide primers for detection of endoglucanase
... within which species differentiation is very difficult, core genes such as gyrB allow differentiation on genetic basis. Compared to other genera, Bacillus species are having conserved 16s rRNA sequences and are difficult to identify at species level using this marker (Stackebrandt and Goebel ...
... within which species differentiation is very difficult, core genes such as gyrB allow differentiation on genetic basis. Compared to other genera, Bacillus species are having conserved 16s rRNA sequences and are difficult to identify at species level using this marker (Stackebrandt and Goebel ...
UNIT V – MENDELIAN GENETICS
... o Change in the nucleotide sequence o May be spontaneous mistakes that occur during replication, repair, or recombination o May be caused by mutagens; for example, x-rays, UV light, carcinogens o If changes involve long stretches of DNA, known as chromosomal mutations o Point mutations – change in a ...
... o Change in the nucleotide sequence o May be spontaneous mistakes that occur during replication, repair, or recombination o May be caused by mutagens; for example, x-rays, UV light, carcinogens o If changes involve long stretches of DNA, known as chromosomal mutations o Point mutations – change in a ...
CGH Microarray Solutions for Genome-Wide Genetic Analysis
... Comparative Genomic Hybridization (CGH) is a technique that measures copy number changes between two DNA samples—a sample and a control. The copy number information can be used to visualize large or small chromosomal aberrations, including very small DNA segments such as microdeletions and microdupl ...
... Comparative Genomic Hybridization (CGH) is a technique that measures copy number changes between two DNA samples—a sample and a control. The copy number information can be used to visualize large or small chromosomal aberrations, including very small DNA segments such as microdeletions and microdupl ...
Gene silencing in mammalian cells and the spread of DNA
... Additional studies provided evidence that DNA methylation patterns are not static in somatic cells, as often assumed, but instead can vary at individual CpG sites (Silva et al., 1993; Turker et al., 1989). For mouse Aprt, we showed that a CpG site located between the Aprt promoter and the methylatio ...
... Additional studies provided evidence that DNA methylation patterns are not static in somatic cells, as often assumed, but instead can vary at individual CpG sites (Silva et al., 1993; Turker et al., 1989). For mouse Aprt, we showed that a CpG site located between the Aprt promoter and the methylatio ...
What Controls Variation in Human Skin Color?
... four “effective factors,’’ in this case, independently segregating genes. Aside from the key word minimal (Harrison and Owen’s data could also be explained by 30--40 genes), one of the more interesting findings was that skin reflectance appeared to be mainly additive. In other words, mean skin refle ...
... four “effective factors,’’ in this case, independently segregating genes. Aside from the key word minimal (Harrison and Owen’s data could also be explained by 30--40 genes), one of the more interesting findings was that skin reflectance appeared to be mainly additive. In other words, mean skin refle ...
An effect of the DGAT1 gene polymorphism on breeding
... Bulls were genotyped with two techniques. Restriction fragment length polymorphism (RFLP) was identified according to Winter at al. [2002]. The PCR product, digested over 12 hours by the CfrI restriction enzyme, was separated on 3% agarose gel. In addition, the multi-temperature single strand confor ...
... Bulls were genotyped with two techniques. Restriction fragment length polymorphism (RFLP) was identified according to Winter at al. [2002]. The PCR product, digested over 12 hours by the CfrI restriction enzyme, was separated on 3% agarose gel. In addition, the multi-temperature single strand confor ...
Role of Tension and Twist in Single
... more complex forms of DNA packaging since multivalent polyamines are associated with stages of the cell cycle during which chromatin is highly compact [10]. Most physical studies of DNA condensation to date have concentrated on free molecules in bulk solution. This neglects the biologically relevant ...
... more complex forms of DNA packaging since multivalent polyamines are associated with stages of the cell cycle during which chromatin is highly compact [10]. Most physical studies of DNA condensation to date have concentrated on free molecules in bulk solution. This neglects the biologically relevant ...
AmpliScribe™ T7 High Yield Transcription Kit
... can also be used as templates, provided that the appropriate promoter has been incorporated into one of the primers used. ...
... can also be used as templates, provided that the appropriate promoter has been incorporated into one of the primers used. ...
Occurrence of phosphate acquisition genes in Prochlorococcus cells
... suggest that lateral gene transfer is a relatively rare event. Alternatively, if phosphate acquisition genes are commonly transferred laterally between Prochlorococcus cells, there should be no linkage between the phylotype (and thereby the core genome of Prochlorococcus) and the presence or absence ...
... suggest that lateral gene transfer is a relatively rare event. Alternatively, if phosphate acquisition genes are commonly transferred laterally between Prochlorococcus cells, there should be no linkage between the phylotype (and thereby the core genome of Prochlorococcus) and the presence or absence ...
Physicochemical studies on interactions between DNA and RNA
... cussed previously (Saucier and Wang, 1972), the observed unwinding could be interpreted as due to a transition of the DNA helix from one form to another, over a much longer region of the helix. © Information Retrieval Limited 1 Falconberg Court London W1V5FG England ...
... cussed previously (Saucier and Wang, 1972), the observed unwinding could be interpreted as due to a transition of the DNA helix from one form to another, over a much longer region of the helix. © Information Retrieval Limited 1 Falconberg Court London W1V5FG England ...
Module 7 – Microbial Molecular Biology and Genetics
... like transcription factors that can bind to specific sequences in double-stranded DNA usually make contacts to the sides of the bases exposed in the major groove. In a DNA double helix, each type of nucleobase on one strand normally interacts with just one type of nucleobase on the other strand. Thi ...
... like transcription factors that can bind to specific sequences in double-stranded DNA usually make contacts to the sides of the bases exposed in the major groove. In a DNA double helix, each type of nucleobase on one strand normally interacts with just one type of nucleobase on the other strand. Thi ...
Nonstationary Functional Optimization
... In each generation the best and the average tness are reported as performance measures. The two problems were run independently. Figure 2 & gure 3 show the best-ofgeneration and the generation average results of the rst problem (table 1), and gure 4 & gure 5 give the corresponding results of th ...
... In each generation the best and the average tness are reported as performance measures. The two problems were run independently. Figure 2 & gure 3 show the best-ofgeneration and the generation average results of the rst problem (table 1), and gure 4 & gure 5 give the corresponding results of th ...
Abstract/Session Information for Program Number 1264
... SNP analysis of control and CCD subjects and cross species sequence analysis, we have identified conserved GATA domain binding sites in the RUNX2 promoter. Because TRPS1 is the only GATA domain transcription factor known to function in skeletogenesis, we analyzed its expression pattern during mouse ...
... SNP analysis of control and CCD subjects and cross species sequence analysis, we have identified conserved GATA domain binding sites in the RUNX2 promoter. Because TRPS1 is the only GATA domain transcription factor known to function in skeletogenesis, we analyzed its expression pattern during mouse ...
Site-specific recombinase technology

Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse