Periplasmic adaptor protein AcrA has a distinct
... The acrA gene was inactivated by insertion of the aph gene conferring kanamycin resistance. The inserted sequence also includes a ribosomal binding site and an inframe start codon to allow expression of downstream acrB. The mutant was designated L823 (Table 2). The disrupted region was transduced in ...
... The acrA gene was inactivated by insertion of the aph gene conferring kanamycin resistance. The inserted sequence also includes a ribosomal binding site and an inframe start codon to allow expression of downstream acrB. The mutant was designated L823 (Table 2). The disrupted region was transduced in ...
The control of shoot branching: an example of plant information
... the axillary shoot apex out into the main stem almost certainly underlies the differentiation of vascular strands connecting the growing vascular network of the lateral shoot to the vascular network in the primary stem. Here, an auxin source – the young leaves in the bud, links to an auxin sink – th ...
... the axillary shoot apex out into the main stem almost certainly underlies the differentiation of vascular strands connecting the growing vascular network of the lateral shoot to the vascular network in the primary stem. Here, an auxin source – the young leaves in the bud, links to an auxin sink – th ...
To on your computer
... ADOMETA http://vitkuplab.cu-genome.org/html/adometa/adometa.html ADoption of Orphan METabolic Activities (Orphan enzyme activities in E. coli, B. subtilis, and S. cerevisiae). ORPHAN ENZYME PROJECT http://www.orphanenzymes.org/ GREP http://bisscat.org/GREP/ Generator of Reaction Equations & Pathways ...
... ADOMETA http://vitkuplab.cu-genome.org/html/adometa/adometa.html ADoption of Orphan METabolic Activities (Orphan enzyme activities in E. coli, B. subtilis, and S. cerevisiae). ORPHAN ENZYME PROJECT http://www.orphanenzymes.org/ GREP http://bisscat.org/GREP/ Generator of Reaction Equations & Pathways ...
Expression and characterization of von Willebrand
... a dimerization defect by using full-length vWF cDNA would nevertheless result in the formation of dimers by disulfide bonding at the intact amino-terminal. Following that strategy, we expressed the carboxy-terminal proteolytic vWF fragment II to assess the isolated dimerization process as previously ...
... a dimerization defect by using full-length vWF cDNA would nevertheless result in the formation of dimers by disulfide bonding at the intact amino-terminal. Following that strategy, we expressed the carboxy-terminal proteolytic vWF fragment II to assess the isolated dimerization process as previously ...
gemini Documentation
... Note: we now recommend splitting variants with multiple alternates and left-aligning, and trimming all variants before loading into gemini. See Step 1. split, left-align, and trim variants for a detailed explanation. Before we can use GEMINI to explore genetic variation, we must first load our VCF f ...
... Note: we now recommend splitting variants with multiple alternates and left-aligning, and trimming all variants before loading into gemini. See Step 1. split, left-align, and trim variants for a detailed explanation. Before we can use GEMINI to explore genetic variation, we must first load our VCF f ...
The Center for Organogenesis | University of Michigan, Ann Arbor
... cell fate decisions in numerous processes (Uwanogho et al., 1995). Sox1, Sox2 and Sox3 are all expressed in the lens (Kamachi et al., 1998). Sox2 and Sox3 expression in the early lens placode is dependent on the optic vesicle and this implies that they are responsive to inductive signals (Kamachi et ...
... cell fate decisions in numerous processes (Uwanogho et al., 1995). Sox1, Sox2 and Sox3 are all expressed in the lens (Kamachi et al., 1998). Sox2 and Sox3 expression in the early lens placode is dependent on the optic vesicle and this implies that they are responsive to inductive signals (Kamachi et ...
Consistency analysis of redundant probe sets on Affymetrix three
... Expression arrays from Affymetrix contain hundreds of thousands of oligonucleotide probes grouped into functional units called probe sets, where each probe set is designed to measure the expression of a known or computationally-predicted mRNA target molecule. Affymetrix sells two commonly-used types ...
... Expression arrays from Affymetrix contain hundreds of thousands of oligonucleotide probes grouped into functional units called probe sets, where each probe set is designed to measure the expression of a known or computationally-predicted mRNA target molecule. Affymetrix sells two commonly-used types ...
p68/DDX5 DEAD-box RNA helicase gene encodes a novel miRNA
... that while p68 RNA helicase activity appears to be important for some functions (e.g., RNA processing), it does not appear to be required for its role as a transcriptional coactivator. The p68 gene contains a large intron (intron 11, 1.2 kb in the human gene), which has been conserved through evolut ...
... that while p68 RNA helicase activity appears to be important for some functions (e.g., RNA processing), it does not appear to be required for its role as a transcriptional coactivator. The p68 gene contains a large intron (intron 11, 1.2 kb in the human gene), which has been conserved through evolut ...
Assessing the role of anammox in a nitrogen contaminated aquifer
... Although nitrogen makes up 78% of our atmosphere, most is unavailable to organisms due to the strong triple bond that holds the two N atoms together. In order to be able to utilize this N, it must be fixed. N fixation can either occur abiotically by lightning or biologically by certain microorganis ...
... Although nitrogen makes up 78% of our atmosphere, most is unavailable to organisms due to the strong triple bond that holds the two N atoms together. In order to be able to utilize this N, it must be fixed. N fixation can either occur abiotically by lightning or biologically by certain microorganis ...
Establishing neuroblast-specific gene expression in the Drosophila
... Fig. 2. Hkb protein is detected in eight neuroblasts per hemisegment in wild-type embryos. All Hkb+ neuroblasts delaminate from Hkb+ neuroectoderm, with the exception of NB 1-1, which becomes Hkb+ midway through its cell lineage (see text for details). Hatched cells, low Hkb; gray cells, high Hkb. U ...
... Fig. 2. Hkb protein is detected in eight neuroblasts per hemisegment in wild-type embryos. All Hkb+ neuroblasts delaminate from Hkb+ neuroectoderm, with the exception of NB 1-1, which becomes Hkb+ midway through its cell lineage (see text for details). Hatched cells, low Hkb; gray cells, high Hkb. U ...
allJoiner
... • The central concept of all.joiner is the identifier, which appears in fields of multiple tables, sometimes even multiple databases. • $gbd is a variable that contains a comma-separated list of genome databases. • An identifier consists of a an identifier line, a required comment in quotes, and a l ...
... • The central concept of all.joiner is the identifier, which appears in fields of multiple tables, sometimes even multiple databases. • $gbd is a variable that contains a comma-separated list of genome databases. • An identifier consists of a an identifier line, a required comment in quotes, and a l ...
Abbott RealTime HIV-1
... A total of 88 clinical specimens, ten of each Group M subtype (A, B, C, D, CRF01-AE, F, CRF02-AG, G) and eight of Group O, were tested with the RealTime HIV-1 assay and by two other HIV-1 quantitative assays referred to as Comparator 1 and Comparator 2. The numbers in parentheses are the number of s ...
... A total of 88 clinical specimens, ten of each Group M subtype (A, B, C, D, CRF01-AE, F, CRF02-AG, G) and eight of Group O, were tested with the RealTime HIV-1 assay and by two other HIV-1 quantitative assays referred to as Comparator 1 and Comparator 2. The numbers in parentheses are the number of s ...
The presence of an RHD pseudogene containing a
... proposed that these techniques could be used for testing all D-negative pregnant women to ascertain whether they require administration of antenatal anti-D immunoglobulin.18,19 Testing would reduce wastage of this valuable resource by avoiding anti-D immunoglobulin administration to women with a D-n ...
... proposed that these techniques could be used for testing all D-negative pregnant women to ascertain whether they require administration of antenatal anti-D immunoglobulin.18,19 Testing would reduce wastage of this valuable resource by avoiding anti-D immunoglobulin administration to women with a D-n ...
Health consequences of adult hypolactasia
... … Lactase persistence varies greatly between different and within populations from almost 0 % in South East Asia to 99 % iin N Northern th E Europe To explain these highly geographic variations in the prevalence of lactase persistence, various researchers have produced some hypotheses to explain the ...
... … Lactase persistence varies greatly between different and within populations from almost 0 % in South East Asia to 99 % iin N Northern th E Europe To explain these highly geographic variations in the prevalence of lactase persistence, various researchers have produced some hypotheses to explain the ...
Daniël van Draanen - Utrecht University Repository
... traits is limited to outer visible traits like eye colour, hair colour and skin texture (WoodRobinson, Lewis, & Leach, 2000). Further findings about educational use of the terms are discussed in paragraph 3.5 of the theoretical basis. According to concept learning theories, students have a prototyp ...
... traits is limited to outer visible traits like eye colour, hair colour and skin texture (WoodRobinson, Lewis, & Leach, 2000). Further findings about educational use of the terms are discussed in paragraph 3.5 of the theoretical basis. According to concept learning theories, students have a prototyp ...
The Genetics of Migraine
... among individuals belonging to different families but also within the same family.13,15,16 Besides familial cases, some sporadic cases of hemiplegic migraine with cerebellar symptoms have also been reported.16,27 Whether FHM has the same pathophysiological mechanisms as other types of migraine with ...
... among individuals belonging to different families but also within the same family.13,15,16 Besides familial cases, some sporadic cases of hemiplegic migraine with cerebellar symptoms have also been reported.16,27 Whether FHM has the same pathophysiological mechanisms as other types of migraine with ...
Current Trends of Emergence and Spread of Vancomycin
... 3.4 Resistance to macrolides, lincosamides and streptogramin B (MLSB) Resistance to MLSB antibiotics is encoded by the widespread erm(B) gene and only occasionally via erm(A) or erm(C)(Roberts et al., 1999). Erm (“erythromycin resistance methylases”) confer resistance by modifying nucleotide A2058 o ...
... 3.4 Resistance to macrolides, lincosamides and streptogramin B (MLSB) Resistance to MLSB antibiotics is encoded by the widespread erm(B) gene and only occasionally via erm(A) or erm(C)(Roberts et al., 1999). Erm (“erythromycin resistance methylases”) confer resistance by modifying nucleotide A2058 o ...
Fulltext PDF - Indian Academy of Sciences
... stmA in the structure or function of the transmembrane sodium channels. Reasons for doubt arise from the observation that double mutants of stmA and para, a structural gene for neuronal voltage-gated sodium channels in Drosophila (Loughney et al. 1989), did not show any phenotypic interaction (Chand ...
... stmA in the structure or function of the transmembrane sodium channels. Reasons for doubt arise from the observation that double mutants of stmA and para, a structural gene for neuronal voltage-gated sodium channels in Drosophila (Loughney et al. 1989), did not show any phenotypic interaction (Chand ...
COLEOPTILE PHOTOTROPISM1 Gene Encoding
... the cpt1 mutant were digested with BamHI, and the fragments were, after self-ligation, subjected to inverse PCR. The sequence of the amplified fragment (;2 kb) was compared with those of the three clones (P0689H05, P0025F02, and OSJNBa0008E01) and the deletion region of 208,845 bp was determined (Fi ...
... the cpt1 mutant were digested with BamHI, and the fragments were, after self-ligation, subjected to inverse PCR. The sequence of the amplified fragment (;2 kb) was compared with those of the three clones (P0689H05, P0025F02, and OSJNBa0008E01) and the deletion region of 208,845 bp was determined (Fi ...
Practice Genetic Problems PMB 220 1. In working with a haploid
... is a tryptophan auxotroph (tryptophan must be added to the media to allow the mutant to grow). A mutation at a second, unlinked locus, su, suppresses the Trp- phenotype of the td mutant so that it can grow on unsupplemented medium. a. ...
... is a tryptophan auxotroph (tryptophan must be added to the media to allow the mutant to grow). A mutation at a second, unlinked locus, su, suppresses the Trp- phenotype of the td mutant so that it can grow on unsupplemented medium. a. ...
(Pan) Salmonella and Salmonella Serotype Typhi–Specific Real
... Salmonella species are important causes of enteritis throughout the world. In addition, Salmonella enterica serotypes Typhi and Paratyphi are important causes of enteric fever in underdeveloped countries that lack adequate sewage disposal and water treatment facilities.1,2 It is standard practice in ...
... Salmonella species are important causes of enteritis throughout the world. In addition, Salmonella enterica serotypes Typhi and Paratyphi are important causes of enteric fever in underdeveloped countries that lack adequate sewage disposal and water treatment facilities.1,2 It is standard practice in ...
Chance and Necessity in Arthur Peacocke`s Scientific Work
... time understood it. Such thoughts must have started to be formed in the mind of Peacocke and lead him on the path of pondering the nature of the interaction of living matter with the non-living world and communion of the whole of creation. At the very root of his subsequent deep thoughts on the natu ...
... time understood it. Such thoughts must have started to be formed in the mind of Peacocke and lead him on the path of pondering the nature of the interaction of living matter with the non-living world and communion of the whole of creation. At the very root of his subsequent deep thoughts on the natu ...
Selective Disruption of Aurora C Kinase Reveals Distinct Functions
... Meiotic cells, however, contain another enzymatic subunit, Aurora C kinase (AURKC), that can function in the CPC in place of AURKB [6,21–25]. AURKB and AURKC are members of a conserved serine-threonine protein kinase family, and are highly similar in sequence within their catalytic domains. Both AUR ...
... Meiotic cells, however, contain another enzymatic subunit, Aurora C kinase (AURKC), that can function in the CPC in place of AURKB [6,21–25]. AURKB and AURKC are members of a conserved serine-threonine protein kinase family, and are highly similar in sequence within their catalytic domains. Both AUR ...
GFF files
... GFF is a format for describing genes and other features associated with DNA, RNA and protein sequences. It was firstly developed at the Sanger center (their current version is 2.0 - http://www.sanger.ac.uk/Software/formats/GFF/). Fields are:
... GFF is a format for describing genes and other features associated with DNA, RNA and protein sequences. It was firstly developed at the Sanger center (their current version is 2.0 - http://www.sanger.ac.uk/Software/formats/GFF/). Fields are:
Site-specific recombinase technology
Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse