Biology
... During DNA replication, the DNA molecule separates into two strands, then produces two new complementary strands following the rules of base pairing. Each strand of the double helix of DNA serves as a template for the new strand. ...
... During DNA replication, the DNA molecule separates into two strands, then produces two new complementary strands following the rules of base pairing. Each strand of the double helix of DNA serves as a template for the new strand. ...
Comparison of the separation of Candida albicans chromosome
... C. albicans chromosomal bands were separated by preparative FIGE using electrophoretic conditions described in Figure 3. Following destaining of the gel, the appropriate bands were visualized under an ultraviolet transilluminator and excised from the gel. DNA in agarose blocks was digested with Eco ...
... C. albicans chromosomal bands were separated by preparative FIGE using electrophoretic conditions described in Figure 3. Following destaining of the gel, the appropriate bands were visualized under an ultraviolet transilluminator and excised from the gel. DNA in agarose blocks was digested with Eco ...
Vorms final version + images
... The early history of classical genetics provides us with a fascinating case of interdisciplinary research. The laying and consolidation of its essential components, in the 1910’s and 1920’s, resulted to a large extent from the integration of two modes of analysis, corresponding to two different disc ...
... The early history of classical genetics provides us with a fascinating case of interdisciplinary research. The laying and consolidation of its essential components, in the 1910’s and 1920’s, resulted to a large extent from the integration of two modes of analysis, corresponding to two different disc ...
Chapter 13 – Meiosis and Sexual Life Cycles
... At metaphase II, nonidentical sister chromatids sort independently from one another, increasing by even more the number of genetic types of daughter cells that are formed by meiosis. ...
... At metaphase II, nonidentical sister chromatids sort independently from one another, increasing by even more the number of genetic types of daughter cells that are formed by meiosis. ...
16S rRNA characterization of Bacillus strain and its
... Testing the KatA gene responsible for hydrogen peroxide tolerance in the two samples showed higher band intensity in the frozen sample when compared to the subcultured sample. This indicates that the hydrogen peroxide tolerance trait is extrachromosomal and has been lost during successive cell divis ...
... Testing the KatA gene responsible for hydrogen peroxide tolerance in the two samples showed higher band intensity in the frozen sample when compared to the subcultured sample. This indicates that the hydrogen peroxide tolerance trait is extrachromosomal and has been lost during successive cell divis ...
Genomic Analysis of Arabidopsis Thaliana
... different chromosomes (2n = 10). The first boom in the isolation of visible mutations occurred in the 1960s and 1970s, when classical genetic mapping with mutational markers was carried out in Arabidopsis. These loci were assigned to five linkage groups in 1983 by Koornneef et al. in the Netherlands ...
... different chromosomes (2n = 10). The first boom in the isolation of visible mutations occurred in the 1960s and 1970s, when classical genetic mapping with mutational markers was carried out in Arabidopsis. These loci were assigned to five linkage groups in 1983 by Koornneef et al. in the Netherlands ...
Problem Set V - Biology 2970
... chromosome that is designated chromosome 21. Such trisomic individuals have 47 chromosomes rather than the normal 46. Down's syndrome patients that have 46 chromosomes are occasionally found, however. Almost always in such cases the long arm of chromosome 21 has been translocated to another chromoso ...
... chromosome that is designated chromosome 21. Such trisomic individuals have 47 chromosomes rather than the normal 46. Down's syndrome patients that have 46 chromosomes are occasionally found, however. Almost always in such cases the long arm of chromosome 21 has been translocated to another chromoso ...
Salmonella typhi mutants defective in anaerobic respiration are
... conditions were isolated. Characterization of their phenotypes and regulatory properties revealed that two of the mutants were unable to use nitrate as a terminal electron acceptor in the absence of oxygen, suggesting that they were defective in nitrate reductase activity. Anaerobic induction of the ...
... conditions were isolated. Characterization of their phenotypes and regulatory properties revealed that two of the mutants were unable to use nitrate as a terminal electron acceptor in the absence of oxygen, suggesting that they were defective in nitrate reductase activity. Anaerobic induction of the ...
Chromosomal Clustering of Periodically Expressed Genes
... ring/early trophozoite, trophozoite/early schizont, schizont and early ring, and a total of 472 oligonucleotides (351 genes) were known to be expressed in one of these stages [1]. Based on Table S2 and Figure 2 of Bozdech study [1], there are 183, 75, 69 and 24 periodically expressed genes in these ...
... ring/early trophozoite, trophozoite/early schizont, schizont and early ring, and a total of 472 oligonucleotides (351 genes) were known to be expressed in one of these stages [1]. Based on Table S2 and Figure 2 of Bozdech study [1], there are 183, 75, 69 and 24 periodically expressed genes in these ...
Biomart/ GENOME ALIGNMENT III
... The comparison of the mouse and human genomes has demonstrated the power of comparative genomics in inferring the evolutionary history of species and in identifying functional regions in genomes. The possibilities for identifying regions under selection are enhanced with the addition of more sequenc ...
... The comparison of the mouse and human genomes has demonstrated the power of comparative genomics in inferring the evolutionary history of species and in identifying functional regions in genomes. The possibilities for identifying regions under selection are enhanced with the addition of more sequenc ...
Case report - HAL
... Sequencing of the APC gene revealed a heterozygous 4 nucleotides deletion at codon 1062, leading to a frameshift. Relative intensity of the mutated peak compared to the normal one was similar in both the tumor and the non-tumor sample, indicating the absence of a loss of heterozygosity at this locus ...
... Sequencing of the APC gene revealed a heterozygous 4 nucleotides deletion at codon 1062, leading to a frameshift. Relative intensity of the mutated peak compared to the normal one was similar in both the tumor and the non-tumor sample, indicating the absence of a loss of heterozygosity at this locus ...
Cytogenetics and Molecular Genetics of Bone and Soft
... The genetic findings in some of these tumors are not dissimilar from the pattern described in this issue for bladder cancer [Sandberg, in press]. Thus, these tumors are associated with a stepwise process of orchestrated genetic changes starting from excess cellular proliferation, malignant transform ...
... The genetic findings in some of these tumors are not dissimilar from the pattern described in this issue for bladder cancer [Sandberg, in press]. Thus, these tumors are associated with a stepwise process of orchestrated genetic changes starting from excess cellular proliferation, malignant transform ...
Laws of Inheritance EnBio
... second trait that Mendel investigated, seed color, as an example. Seed color is governed by a single gene with two alleles. The yellow-seed allele is dominant and the green-seed allele is recessive. When true-breeding plants were cross-fertilized, in which one parent had yellow seeds and one had gre ...
... second trait that Mendel investigated, seed color, as an example. Seed color is governed by a single gene with two alleles. The yellow-seed allele is dominant and the green-seed allele is recessive. When true-breeding plants were cross-fertilized, in which one parent had yellow seeds and one had gre ...
Ingestion of bacterially expressed dsRNAs can produce specific and
... A variety of species exhibit a defense response in which a double stranded RNA (dsRNA) `trigger' produces a premature loss of endogenous RNAs with extended regions of sequence identity to the trigger (see Fire, 1999 for a review). A striking feature of dsRNA-triggered genetic interference processes ...
... A variety of species exhibit a defense response in which a double stranded RNA (dsRNA) `trigger' produces a premature loss of endogenous RNAs with extended regions of sequence identity to the trigger (see Fire, 1999 for a review). A striking feature of dsRNA-triggered genetic interference processes ...
PDF
... tively. We find that the accuracy of FirstEF is significantly higher for CpG-related than non-CpG-related genes. To study the performance of FirstEF on the genome scale, we ran it on human chromosomes 21 and 22 and compared the predictions with the experimentally chromosomes 21 and 22. We downloaded ...
... tively. We find that the accuracy of FirstEF is significantly higher for CpG-related than non-CpG-related genes. To study the performance of FirstEF on the genome scale, we ran it on human chromosomes 21 and 22 and compared the predictions with the experimentally chromosomes 21 and 22. We downloaded ...
Divergent Evolution of Duplicate Genes Leads to Genetic
... science of evolution as well as to plant breeding. Whether these incompatibilities mostly appear concurrently with speciation (arising, for example, in geographically isolated populations) or after speciation has occurred as a consequence of their divergence remains a considerable question (4); it i ...
... science of evolution as well as to plant breeding. Whether these incompatibilities mostly appear concurrently with speciation (arising, for example, in geographically isolated populations) or after speciation has occurred as a consequence of their divergence remains a considerable question (4); it i ...
Bacteriophage A cloning system for the construction of
... removed from the genome of AORF8. Sites for these endonucleases are present within the multiple cloning site of AORF8. We report a method for preparing cDNAs that can be cloned in a single orientation in our phage vector. The method utilizes the synthesis of double-stranded cDNA, including priming o ...
... removed from the genome of AORF8. Sites for these endonucleases are present within the multiple cloning site of AORF8. We report a method for preparing cDNAs that can be cloned in a single orientation in our phage vector. The method utilizes the synthesis of double-stranded cDNA, including priming o ...
a role for mitochondrial enzymes in inherited neoplasia and beyond
... The diagram shows the five complexes that are involved in the mitochondrial electron-transport chain. Complexes I–IV are the electron-transport complexes, whereas complex V synthesizes adenosine triphosphate (ATP). Electrons are passed down the four complexes (black arrows) to molecular oxygen and t ...
... The diagram shows the five complexes that are involved in the mitochondrial electron-transport chain. Complexes I–IV are the electron-transport complexes, whereas complex V synthesizes adenosine triphosphate (ATP). Electrons are passed down the four complexes (black arrows) to molecular oxygen and t ...
The ultrasound detection of chromosomal anomalies
... cells of the body that contains two sets of chromosome the cells are called diploid Chromosome are displayed in a karyotype in order or decreasing size, with the small arm “p” on top and the longer arm “q” at the bottom. The last pair of chromosome represented in a karyotype is the sex chromosomes. ...
... cells of the body that contains two sets of chromosome the cells are called diploid Chromosome are displayed in a karyotype in order or decreasing size, with the small arm “p” on top and the longer arm “q” at the bottom. The last pair of chromosome represented in a karyotype is the sex chromosomes. ...
Amphioxus
... alternative splicing event. BfMT1 is a polyvalent metal binding peptide that coordinates any of the studied metal ions (Zn, Cd or Cu) rendering complexes stable enough to last in physiological environments, which is fully concordant with the constitutive expression of its gene, and therefore, with a ...
... alternative splicing event. BfMT1 is a polyvalent metal binding peptide that coordinates any of the studied metal ions (Zn, Cd or Cu) rendering complexes stable enough to last in physiological environments, which is fully concordant with the constitutive expression of its gene, and therefore, with a ...
Diapositive 1
... chromosomes composed of a thin ribbon molecule that you all know as the DNA. What is the role of the DNA? This is the databank, the hard drive of your genetic patrimony what we call the genome which will create your individuality. The entire genome is present in all cells of your body and contains m ...
... chromosomes composed of a thin ribbon molecule that you all know as the DNA. What is the role of the DNA? This is the databank, the hard drive of your genetic patrimony what we call the genome which will create your individuality. The entire genome is present in all cells of your body and contains m ...
The Rapid Evolution of X-linked Male
... the faster-X evolution is mostly due to relaxed selective constraints associated with reduced effective population size and/or the result of positive selection remains yet to be fully resolved and could be species specific, but genes expressed at higher levels in males than in females (i.e., male-bi ...
... the faster-X evolution is mostly due to relaxed selective constraints associated with reduced effective population size and/or the result of positive selection remains yet to be fully resolved and could be species specific, but genes expressed at higher levels in males than in females (i.e., male-bi ...
- Lab Fabiana Perocchi
... shown here for a SNP marked by an asterisk. (B) Modeling the hybridization intensity. Consider a two-allelic transcript with two indels and one SNP as shown in the lower part. The S allele is at expression level hS and the Y allele at hY. Hybridization intensities of the common probes are ideally ex ...
... shown here for a SNP marked by an asterisk. (B) Modeling the hybridization intensity. Consider a two-allelic transcript with two indels and one SNP as shown in the lower part. The S allele is at expression level hS and the Y allele at hY. Hybridization intensities of the common probes are ideally ex ...
Validating therapeutic targets through human genetics
... lead to toxicity (for example, steroid-induced elevated blood glucose levels). For simplicity, adverse events associated with low cortisol levels are not shown. c | Low-density lipoprotein (LDL) levels and cardiovascular disease. Variants in different genes can lead to variations in the levels of LD ...
... lead to toxicity (for example, steroid-induced elevated blood glucose levels). For simplicity, adverse events associated with low cortisol levels are not shown. c | Low-density lipoprotein (LDL) levels and cardiovascular disease. Variants in different genes can lead to variations in the levels of LD ...
Site-specific recombinase technology
Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse