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Plant–nematode interactions
Plant–nematode interactions

... plant-parasitic nematode species, have been produced [42]. Existing EST collections from plant-parasitic nematodes are mostly derived from eggs and infective juveniles, but future projects will likely expand to include the parasitic stages [27,34,42]. DNA-sequence analyses of these ESTs have shown ...
PcGs and Hox genes - Development
PcGs and Hox genes - Development

... Su(z)2 gene (Wu and Howe, 1995); we shall refer to this double mutant as Psc-Su(z)2. Similarly, for ph we used an allele that carries lesions in both the proximal and the distal ph transcription unit and we shall refer to the double mutant as ph0 (Dura et al., 1987). In all experiments, PcG mutant c ...
Strategies for Improving Soluble Protein Production in E. coli
Strategies for Improving Soluble Protein Production in E. coli

... The Protein Expression Host of Choice based on Pubmed Proportion of Recombinant Genes Expressed in Different Organisms ...
GENE-CBR - Indiana University School of Informatics
GENE-CBR - Indiana University School of Informatics

... Machine Learning and Data Mining techniques but … Its capacity for generating enormous amounts of data is, however, also an handicap: great amount of data belonging to each individual (thousands of genes) • efficiency and memory problems ...
Bioinformatics Database Worksheet
Bioinformatics Database Worksheet

... (for blue, green, and red light detection). Four hits look like visual pigments, which probably does not surprise you. To the left of each entry is the chromosome number, allowing you to tell which red mark corresponds to each entry. Note that several hits are on the X chromosome, one of the sex-det ...
Transcriptional Repression of Hox Genes by C. elegans HP1/HPL
Transcriptional Repression of Hox Genes by C. elegans HP1/HPL

... Here, we investigate the role of HP1 and H1 in development using the nematode C. elegans as a model system. We focus on the underlying molecular mechanisms of gene co-regulation by H1 and HP1. We show that the loss of both HP1 and H1 alters the expression of a small subset of genes. C. elegans HP1 a ...
Hd6, a rice quantitative trait locus involved in photoperiod
Hd6, a rice quantitative trait locus involved in photoperiod

... In this region, we found only one EST (C10214), which showed high homology with the CK2␣ gene of Arabidopsis and maize. Second, the CK2␣ allele of Nipponbare has a premature stop codon that is not found in the same position in the Kasalath allele (Fig. 4B). The Kasalath mRNA has an ORF (333 aa) whos ...
Bioinformatics
Bioinformatics

... print "`$genome' has a gene count of $gene_counts { $genome }\n";} Refer to genes.pl ...
Becker Muscular Dystrophy (BMD)
Becker Muscular Dystrophy (BMD)

... which is found on the X chromosome. The dystrophin gene normally makes a protein that is essential for keeping our muscles healthy and strong. If the dystrophin gene has an alteration in it, then the protein is not produced correctly and the body may not be able to replace damaged muscle tissue or g ...
Cancer Prone Disease Section Hereditary multiple exostoses (HME) in Oncology and Haematology
Cancer Prone Disease Section Hereditary multiple exostoses (HME) in Oncology and Haematology

Gene affecting stature and body size in mammalian species
Gene affecting stature and body size in mammalian species

... infinite number of loci, each with infinitesimal effect, is not literally true but it ...
Cryptochrome 1 controls tomato development
Cryptochrome 1 controls tomato development

... Cryptochrome control of tomato development 555 elongation, stem growth and internode elongation, leaf and cotyledon expansion, B-dependent gene expression, and anthocyanin accumulation (Ahmad and Cashmore, 1993; Ahmad and Cashmore, 1996; Fuglevand et al., 1996; Koornneef et al., 1980) and, in combi ...
Extensive tRNA gene changes in synthetic Brassica
Extensive tRNA gene changes in synthetic Brassica

... A total of 1157 putative tRNA genes were predicted from B. rapa genome sequences using tRNA predicting program “tRNAscan-SE”. These genes were classified into 23 groups according to the type of tRNA isoacceptor (see Fig. 1 for detailed information). There were 67 intron-containing tRNA genes, belon ...
Microarray data normalization and data transformation
Microarray data normalization and data transformation

... • Although this may not produce any significant changes in relative expression levels between conditions for highly expressed genes, it can have profound effect on estimates for genes expressed at low to moderate levels (2) Chooses to subtract background – To use the fluorescence of the area surroun ...
Thrombocytopenia-absent radius (TAR) syndrome due to compound
Thrombocytopenia-absent radius (TAR) syndrome due to compound

... that may be episodic, congenital skeletal deformities including bilateral absence of radius, shortening and deformity of the ulnae, and occasionally absence of all the long bones in the arm. The fingers and thumbs are always present, while other skeletal anomalies are frequent [11]. A chromosome 1q2 ...
Mbd3, a component of the NuRD co-repressor
Mbd3, a component of the NuRD co-repressor

... is, they are capable of giving rise to all embryonic and extraembryonic tissues (Rossant, 1976). At the morula stage, the embryo begins to compact and by the blastocyst stage the outer cells form an extra-embryonic cell layer called the trophectoderm, while the inside cells form the inner cell mass ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)

... biological macromolecules, including DNA. Several studies have demonstrated that oxidative stress plays an important role in the pathogenesis of cardiovascular alterations observed in diabetic patients and that hyperglycemia is the causal link between diabetes and increased oxidative stress. Objecti ...
Adherin - Semantic Scholar
Adherin - Semantic Scholar

... A recent flurry of papers provides new insights into how the adherin proteins (Table 1) regulate diverse chromosomal functions and development. Adherin functions were originally revealed by genetic mutations in fungi: the rad9-1 adherin mutant of the mushroom Coprinus cinereus is defective in meioti ...
PDF
PDF

... is, they are capable of giving rise to all embryonic and extraembryonic tissues (Rossant, 1976). At the morula stage, the embryo begins to compact and by the blastocyst stage the outer cells form an extra-embryonic cell layer called the trophectoderm, while the inside cells form the inner cell mass ...
Chapter 18: Gene Mutation and DNA Repair
Chapter 18: Gene Mutation and DNA Repair

... involved the use of a velvet cloth to make copies of a master plate. 6. A region of a gene that is more likely to mutate than another region is called a ________. ...
Controversial cases of human gender identification by amelogenin test
Controversial cases of human gender identification by amelogenin test

... Received: October 16, 2007 / Accepted: November 15, 2007 ...
cg12 Expression Is Specifically Linked to Infection of
cg12 Expression Is Specifically Linked to Infection of

... symbiotic interaction with Frankia (Franche et al. 1998b; Laplaze et al. 2000a, 2002). In order to monitor the activity of the cg12 promoter during the early stages of Casuarina infection by Frankia, transcriptional fusions of the 5‡ upstream region with gus and gfp reporter genes were constructed. ...
Teaching scheme - Pearson Schools and FE Colleges
Teaching scheme - Pearson Schools and FE Colleges

... which a nucleotide sequence codes for the amino acid sequence in a polypeptide. OCR Scheme of Work topic outlines • Describe, with the aid of diagrams, how the sequence of nucleotides within a gene is used F215 Control, genomes and environment to construct a polypeptide – include the roles 5.1.1 Cel ...
one
one

... separately expressed, and both phenotypes are also completely expressed. Human blood type is an example of both codominance and a multiple allele trait. The alleles for blood types A and B are codominant, which can be expressed as an AB blood type. The allele for type O blood is recessive to the oth ...
Evolution of Closely Linked Gene Pairs in
Evolution of Closely Linked Gene Pairs in

... it becomes difficult to separate the pair as insertion of intergenic DNA, such as a repetitive element, would disturb expression of both genes. H2h gene pairs would thus slowly accumulate during evolution. This explanation is supported by the higher than average evolutionary conservation of h2h pair ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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