• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
The Human Genome
The Human Genome

... DNA is cut with restriction enzyme Small fragments separated by Gel Electrophoresis. Fragments with variable regions are detected with probe resulting in DNA bands of various sizes. Look for pattern. ...
Document
Document

... 7.3 Gene Linkage and Mapping • Cross-over frequencies can be converted into map units. – gene A and gene B cross over 6.0 percent of the time – gene B and gene C cross over 12.5 percent of the time – gene A and gene C cross over 18.5 percent of the time ...
7.3 Gene Linkage and Mapping KEY CONCEPT chromosomes.
7.3 Gene Linkage and Mapping KEY CONCEPT chromosomes.

Heredity
Heredity

... reproduction in influencing genetic variability in a population. 3.1.12.B3: Analyze gene expression at the molecular level. Explain the impact of environmental factors on gene expression. ...
Choose your target
Choose your target

... the targeting construct and the endogenous chromosomal sequence is very similar to that observed in mammalian cells, including mouse embryonic stem (ES) cells7,8. In both yeast and mammalian cells, linear DNA molecules are more effective substrates for gene-targeting reactions than are circular mole ...
File
File

... Gene expression • In prokaryotes the coding sequence is a continuous piece of DNA (not interrupted) and the majority of these genes are organized into operons which are group of genes whose products have related functions and transcribed as one unit. • In eukaryotic genes are transcribed individual ...
Document
Document

... Tools as beaks. They evolved to fit their environment Looking different=variation, the starting point for change in nature Evolution by natural selection: the fit get fitter, the variations that are not as adaptable, die 150 years later his ideas are still respected as ture Dark mice live on dark ro ...
Study Guide
Study Guide

... b. Where do sperms get energy to propel itself? ii. Egg – Form and Function 1. Why are eggs so large? What is the role of the vitelline membrane? b. Early Cleavage to Blastula– What is the purpose of this first phase of development? i. What do you start with and what do you end up with before cells ...
Slide 1
Slide 1

... 1 (24 pt) The following statements relate to our class discussion of organelle genomes. For each statement, indicate whether the statement applies to the plastid genome (pt), the plant mitochondrial genome (mit), both plastid and plant mitochondrial genomes (both), or neither of these genomes (none) ...
30. genetic disorders 31. pedigree 32. Punnett Square
30. genetic disorders 31. pedigree 32. Punnett Square

... 41. mutation ...
2054, Chap. 13, page 1 I. Microbial Recombination and Plasmids
2054, Chap. 13, page 1 I. Microbial Recombination and Plasmids

... 7. transposons have been observed in eukaryotes, bacteria, Archaea, yeast, maize, Drosophila, and humans 8. transposons contribute to genetic diversity D. conjugation = transfer of DNA between bacteria in direct contact 1. depends on plasmids (free or integrated) 2. Lederberg and Tatum mixed multipl ...
Document
Document

... _____ 1. A gene is expressed when it is a. present in the genome of an individual. b. prevented from interacting with RNA polymerase. c. transcribed into mRNA. d. duplicated during the replication of DNA. _____ 2. In the lac operon of E. coli, lactose functions as a. a promoter. b.an operator. c. a ...
Chorionic Gonadotropin (CG) 태반성 성선자극호르몬
Chorionic Gonadotropin (CG) 태반성 성선자극호르몬

... Chorionic Gonadotropin Has a Recent Origin Within Primates and an Evolutionary History of Selection Glenn A. Maston & Maryellen Ruvolo Department of Anthropology, Harvard University, Cambridge, Massachusetts Abstract ...
Document
Document

... Write out the antiparallel strand in the 5’ to 3’ orientation:_________________ 2) Name the 4 most common RNA bases (spell out) ___________ _____________ ______________ ___________ 3) A fifth common RNA base ________ is used in tRNA for wobble. 4) Name the 5 most common DNA bases (spell out)________ ...
Control of Gene Expression (PowerPoint) Madison 2009
Control of Gene Expression (PowerPoint) Madison 2009

... a) Students will be able to describe a method to show that the DNA content of different cell types is identical. b) Students will be able to explain why an individual cell can produce an entire organism 2) Students will understand how mechanisms of transcriptional regulation lead to differential gen ...
4.1 Le Noyau
4.1 Le Noyau

... result of how the bases on the DNA molecule are arranged. • A joins with T • G joins with C • But the order and number of these bases can vary greatly within the DNA molecule ...
Microarrays = Gene Chips
Microarrays = Gene Chips

... 8. If the PCR product has stuck on it will glow 9. The computer can then say which of the bacterial species the PCR products have stuck to and this indicates which species are present in the sample ...
Glossary 29Sept2012_Genetics
Glossary 29Sept2012_Genetics

... gene. The order of a DNA string's base pairs determines which proteins are produced, and thus the function of a particular cell. Trait: The physical characteristics, like eye color or nose shape, which are determined by inherited genes. ...
DIY DNA.Study Plan-Obj
DIY DNA.Study Plan-Obj

... 2. Indicate, in a general way, the nature of viruses (structure, sizes relative to other cells, shapes, and how they function). 3. Indicate what is needed in cells so they can repeatedly carry out a complex series of chemical reactions in an exact order. 4. Recognize where in the cell such informati ...
BIOL. 303 EXAM III 11/30/07
BIOL. 303 EXAM III 11/30/07

... What modification neutralizes the charges on histones and loosens up the interactions between histones and DNA? A. phosphorylation B. methylation C. acetylation D. polyadenylation ...
Biology 303 EXAM II 3/14/00 NAME
Biology 303 EXAM II 3/14/00 NAME

... What modification neutralizes the charges on histones and loosens up the interactions between histones and DNA? A. phosphorylation B. methylation C. acetylation D. polyadenylation ...
Ghost in Your Genes Viewing Guide
Ghost in Your Genes Viewing Guide

... BACKGROUND: "Ghost in Your Genes" focuses on epigenetic "switches" that turn genes "on" or "off." But not all switches are epigenetic; some are genetic. That is, other genes within the chromosome turn genes on or off. In an animal's embryonic stage, these gene switches play a main role in laying out ...
REVIEW OF MOLECULAR GENETICS - Pascack Valley Regional
REVIEW OF MOLECULAR GENETICS - Pascack Valley Regional

... TIFF (Uncompressed) decompressor are needed to see this picture. ...
Document
Document

... 4. Influences timing of replication and choice of origin utilized Enhancer 1. Stimulates transcription in an orientation-independent manner 2. Classical enhancers function independently of orientation and distance Promoter 1. Region of DNA at which RNA polymerase binds and initiates transcription Re ...
MUTATIONS, MUTAGENESIS, AND CARCINOGENESIS
MUTATIONS, MUTAGENESIS, AND CARCINOGENESIS

... Carcinogenesis: sunlight and cancer ! Cancers associated with inactivation (or activation) of genes ! First evidence that DNA damage induces cancer came from study of UV radiation and skin cancer ...
< 1 ... 1244 1245 1246 1247 1248 1249 1250 1251 1252 ... 1288 >

Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report