N. crassa et al. However, despite the speed and
... Increasing the efficiency of random isolates by using multiply marked testers: Rather that testing an unknown by crossing it to markers in the seven linkage groups one or two at a time, it is more efficient to use multiply marked strains. Testers have been developed that incorporate markers tagging ...
... Increasing the efficiency of random isolates by using multiply marked testers: Rather that testing an unknown by crossing it to markers in the seven linkage groups one or two at a time, it is more efficient to use multiply marked strains. Testers have been developed that incorporate markers tagging ...
Document
... organism’s DNA. – Includes genes (control traits) and noncoding DNA organized in chromosomes. ...
... organism’s DNA. – Includes genes (control traits) and noncoding DNA organized in chromosomes. ...
2006 - Genetics, development and evolution of adaptive
... ‘hood’ was selected to be small in some lines and large in others. Castle initially thought these size differences reflected different alleles of the major gene responsible for hooding; however, Wright showed that so-called modifier genes were responsible for variation in hood size, providing the fi ...
... ‘hood’ was selected to be small in some lines and large in others. Castle initially thought these size differences reflected different alleles of the major gene responsible for hooding; however, Wright showed that so-called modifier genes were responsible for variation in hood size, providing the fi ...
TEL Gene Is Involved in Myelodysplastic Syndromes
... findings (splenomegaly, monocytosis, eosinophilia, and absence of Ph chromosome and breakpoint cluster region rearrangement), three of the four cases (no. 1 to 3) presented were diagnosed as CMML. The fourth case presented as MDS with pancytopenia and only 2% of blasts in the BM (RA). However, an in ...
... findings (splenomegaly, monocytosis, eosinophilia, and absence of Ph chromosome and breakpoint cluster region rearrangement), three of the four cases (no. 1 to 3) presented were diagnosed as CMML. The fourth case presented as MDS with pancytopenia and only 2% of blasts in the BM (RA). However, an in ...
Woods Hole – Zebrafish Genetics and Development Bioinformatics
... pattern consistent with the in situ data: expression in some tissues is absent, and many tissues express GFP where the gene is not normally expressed. How might you explain these results? You decide to make a new reporter line by BAC recombination: you will obtain a large (~200kb) chunk of genomic D ...
... pattern consistent with the in situ data: expression in some tissues is absent, and many tissues express GFP where the gene is not normally expressed. How might you explain these results? You decide to make a new reporter line by BAC recombination: you will obtain a large (~200kb) chunk of genomic D ...
Pre-made tetracycline regulator (TetR) expression lentiviral
... induce expression of the target. (Note: vary the final concentration of tetracycline from 0.01 ug/ml to 1 ug/ml to obtain different expression levels of target). Method B: co-transduce TetR lentiviral particles and expression particles for tetracycline inducible expression: 1. Plate cells in 0.5 ml ...
... induce expression of the target. (Note: vary the final concentration of tetracycline from 0.01 ug/ml to 1 ug/ml to obtain different expression levels of target). Method B: co-transduce TetR lentiviral particles and expression particles for tetracycline inducible expression: 1. Plate cells in 0.5 ml ...
Evolution of Closely Linked Gene Pairs in
... functional entity, a monocistronic coding sequence of which the expression is controlled by an autonomous promoter. In fact, there is a significant clustering of genes in the mammalian genome where the genes in these clusters show coordinate expression (Hurst et al. 2004; The FANTOM Consortium 2005; ...
... functional entity, a monocistronic coding sequence of which the expression is controlled by an autonomous promoter. In fact, there is a significant clustering of genes in the mammalian genome where the genes in these clusters show coordinate expression (Hurst et al. 2004; The FANTOM Consortium 2005; ...
Genetic Characterization and Inheritance of Belly Spot
... Coat color studies are important because in some breeds only certain colors are permitted; another reason is that some coat colors are associated with diseases. The purpose of this master thesis is to shed some light on the inheritance pattern of the splashed white coat color and belly spot in horse ...
... Coat color studies are important because in some breeds only certain colors are permitted; another reason is that some coat colors are associated with diseases. The purpose of this master thesis is to shed some light on the inheritance pattern of the splashed white coat color and belly spot in horse ...
PDF
... sequenced in order to avoid any mutation in this region. The hybrid 6.5-kb Ndn-H19 mini-transgene was excised from the pBS vector by a NotI/SalI digestion and agarose gel purified. The DNA was further purified on an ELUTIP-D column (Schleicher & Schuell, Germany), as described in the manufacturer’s ...
... sequenced in order to avoid any mutation in this region. The hybrid 6.5-kb Ndn-H19 mini-transgene was excised from the pBS vector by a NotI/SalI digestion and agarose gel purified. The DNA was further purified on an ELUTIP-D column (Schleicher & Schuell, Germany), as described in the manufacturer’s ...
Respiration Worksheet
... have a defect in bone growth of the long bones, the arms and legs. As a result, the average height for Achondroplasia dwarves is about 4’ tall. Dwarfism is caused by one dominant allele, D. However, two dominant D alleles causes death, termed double dominant lethality. Most autosomal disorders are c ...
... have a defect in bone growth of the long bones, the arms and legs. As a result, the average height for Achondroplasia dwarves is about 4’ tall. Dwarfism is caused by one dominant allele, D. However, two dominant D alleles causes death, termed double dominant lethality. Most autosomal disorders are c ...
Mouse Fur Color Slides - Evo-Ed
... in no way affected by the license. Other Rights — In no way are any of the following rights affected by the license: Your fair dealing or fair use rights, or other applicable copyright exceptions and limitations; The author's moral rights; Rights other persons may have either in the work itself or i ...
... in no way affected by the license. Other Rights — In no way are any of the following rights affected by the license: Your fair dealing or fair use rights, or other applicable copyright exceptions and limitations; The author's moral rights; Rights other persons may have either in the work itself or i ...
- BioMed Central
... microarray technology allows for the investigation of all genes of an organism under various conditions, resulting in the generation of a massive amount of expression data. One of the greatest challenge we are faced with is to then analyse the data as a whole and extract the meaningful relationships ...
... microarray technology allows for the investigation of all genes of an organism under various conditions, resulting in the generation of a massive amount of expression data. One of the greatest challenge we are faced with is to then analyse the data as a whole and extract the meaningful relationships ...
Definition of historical models of gene function and their relation to
... and become what is called a scientific model (Gilbert et al. 1998). When observational data come in conflict with a model and no easy correspondence can be found between the model and existing data the scientists often are convinced that the anomaly is real and that the model needs revision (Wimsatt ...
... and become what is called a scientific model (Gilbert et al. 1998). When observational data come in conflict with a model and no easy correspondence can be found between the model and existing data the scientists often are convinced that the anomaly is real and that the model needs revision (Wimsatt ...
AnsteadSeniorHonorsThesis
... associated with chromatin following entry of cells into quiescence, making Mcms effective markers of proliferation (Madine, M. 2000). However, this study focused on all of the Mcm proteins, rather than Mcm10 specifically. This serves to represent the deficiency in our knowledge of Mcm10 in multicell ...
... associated with chromatin following entry of cells into quiescence, making Mcms effective markers of proliferation (Madine, M. 2000). However, this study focused on all of the Mcm proteins, rather than Mcm10 specifically. This serves to represent the deficiency in our knowledge of Mcm10 in multicell ...
interPopula: a Python API to access the HapMap Project dataset
... are the exon positions (17 in the case of Lactase). Interpreting the results of this specific application of interPopula is beyond the scope of this manuscript but at least two different interpretations are possible: (i) SNPs where Fst is above approximately 0.45 are candidates for positive selectio ...
... are the exon positions (17 in the case of Lactase). Interpreting the results of this specific application of interPopula is beyond the scope of this manuscript but at least two different interpretations are possible: (i) SNPs where Fst is above approximately 0.45 are candidates for positive selectio ...
Coevolution of an aminoacyl-tRNA synthetase with its tRNA substrates
... In bacteria, the ND Bacillus subtilis GluRS has been extensively studied (9). Due to the lack of a canonical glutaminyltRNA synthetase (GlnRS) in this organism, the ND-GluRS is an essential enzyme in Gln-tRNA formation as it generates GlutRNAGln. This product is then converted to Gln-tRNAGln by Glu- ...
... In bacteria, the ND Bacillus subtilis GluRS has been extensively studied (9). Due to the lack of a canonical glutaminyltRNA synthetase (GlnRS) in this organism, the ND-GluRS is an essential enzyme in Gln-tRNA formation as it generates GlutRNAGln. This product is then converted to Gln-tRNAGln by Glu- ...
View/Open
... occur in discrete or distinct phenotypic classes and exhibit discontinuous variation in a population. Inheritance studies have shown that variation for each qualitative trait in a population is under the genetic control of two or more alleles of a single major gene with high heritability as environ ...
... occur in discrete or distinct phenotypic classes and exhibit discontinuous variation in a population. Inheritance studies have shown that variation for each qualitative trait in a population is under the genetic control of two or more alleles of a single major gene with high heritability as environ ...
The nucleotide sequence of Saccharomyces cerevisiae
... FASTA score is lowered to 150, which is a significant value in many cases, another 20 ORFs (3.5 %) could be envisaged to be of predictable function, raising the total number of ORFs of known or predictable function to 312 (54.6 %). Of the remaining ORFs, 74 (12.9 %) are similar to protein sequences ...
... FASTA score is lowered to 150, which is a significant value in many cases, another 20 ORFs (3.5 %) could be envisaged to be of predictable function, raising the total number of ORFs of known or predictable function to 312 (54.6 %). Of the remaining ORFs, 74 (12.9 %) are similar to protein sequences ...
ARGONAUTE1 Acts in Arabidopsis Root Radial
... radial stratification (Fig. 3I). Similar defects were observed in ago1-101 shr-2 double mutant roots (Fig. 3J). Other than the root radial patterning defects, phenotypes were similar to those described for each single mutant (Di Laurenzio et al. 1996, Bohmert et al. 1998, Helariutta et al. 2000). In ...
... radial stratification (Fig. 3I). Similar defects were observed in ago1-101 shr-2 double mutant roots (Fig. 3J). Other than the root radial patterning defects, phenotypes were similar to those described for each single mutant (Di Laurenzio et al. 1996, Bohmert et al. 1998, Helariutta et al. 2000). In ...
The role of Dby mRNA in early development of male mouse zygotes
... X-inactivation and has 91.7% sequence identity at the protein level in humans. Slightly different from the human DBY gene, mouse Dby has a structural homolog called Ddx3x on the X chromosome and an autosomal homolog, D1Pas1, on mouse chromosome 1 [8, 9]. Furthermore, the spermatogenesis-specific fun ...
... X-inactivation and has 91.7% sequence identity at the protein level in humans. Slightly different from the human DBY gene, mouse Dby has a structural homolog called Ddx3x on the X chromosome and an autosomal homolog, D1Pas1, on mouse chromosome 1 [8, 9]. Furthermore, the spermatogenesis-specific fun ...
4. The cell’s supply of ADP, P , and NAD
... potential would change. This is also why cells don’t store glucose. The cells would become hypertonic to the fluid around them and could pick up enough water to burst. b. Given that cells can’t store ATP for long periods of time, how do they store energy? Instead of storing ATP, cells tend to store ...
... potential would change. This is also why cells don’t store glucose. The cells would become hypertonic to the fluid around them and could pick up enough water to burst. b. Given that cells can’t store ATP for long periods of time, how do they store energy? Instead of storing ATP, cells tend to store ...
Protocadherin-1: epithelial barrier dysfunction in asthma and eczema Grissel Faura Tellez
... Caucasian subjects did not reveal strong evidence for LD between the various SNPs (fig. 1), indicating that these SNPs do not represent a single genetic signal but apparently make individual, independent contributions to disease susceptibility. Another explanation for the identification of multiple ...
... Caucasian subjects did not reveal strong evidence for LD between the various SNPs (fig. 1), indicating that these SNPs do not represent a single genetic signal but apparently make individual, independent contributions to disease susceptibility. Another explanation for the identification of multiple ...
Gene expression profiling
In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.