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Aequatus User Guide
Aequatus User Guide

... of a common species. Aequatus works on top of the Ensembl Compara and Core database schema. • Aequatus uses precalculated gene family information and genomic alignments data in the form of CIGAR strings, from Ensembl Compara, and cross-references these sequences to Ensembl Core databases for each sp ...
identification of tendon and ligament specific genes
identification of tendon and ligament specific genes

... For each array, all probe sets were normalized to a mean signal intensity value of 100. The default GCOS statistical values were used for all analysis. A gene was considered detectable if the mean expression in any tissue was greater than 100 signal units and the percentage of samples with a Present ...
Genotypes and Phenotypes Genetic Foundations Boy or Girl
Genotypes and Phenotypes Genetic Foundations Boy or Girl

... Environmental Influences on Gene Expression • The relationship between heredity and environment is not a one-way street, from genes to environment to behavior. • Rather, it is bidirectional; genes affect children’s behavior and experiences, but their experiences and behavior also affect gene express ...
genes
genes

... The fat cat ate the rat. change one letter ...
Document
Document

... Motor symptoms in one particular mouse model improved within a month of treatment, and continued to improve until the HD mice looked no different from normal mice. When older mice with more symptoms were treated, their motor and behavioral symptoms did improve, but it took much longer for improvemen ...
File
File

... The X2 (chi-squared) Test • Not only do we have to look at the probabilities, we should also look at the degrees of freedom in the results. This value takes into account the number of comparisons made. • To work out the number of degrees of freedom, calculate the number of classes date minus 1. (Th ...
Evolution by Gene Duplication
Evolution by Gene Duplication

... typically in higher trophic levels, drift becomes a much more significant factor, and frequently selection does not succeed. ...
Correlation of Age, Degeneration, and Biomechanical Properties of
Correlation of Age, Degeneration, and Biomechanical Properties of

... discs according to the system described in Boos et al (Spine 2002; 27: 2631-44), and a single numerical value for degeneration was calculated for each disc by summing the scores of the individual histological categories. For mechanical testing L3-L4 discs were removed from the spines as motion segme ...
2-22 and 2-23 Genetics
2-22 and 2-23 Genetics

... Genes, which are located on chromosomes B Cells, which are located in genes C Genes, which are located in the cell membrane of each cell D Specialized cells, which are located in ...
Genetics Video
Genetics Video

... 7. DNA is contained in the n___________ of a cell. 8. G_________ are packed into c_____________________. 9. Mom tells Richie: Genes are the set of __________________ _______________ that get passed down from _____________to child. In the process, of course, the genetic material is __________________ ...
Congenital And Genetic Disorders
Congenital And Genetic Disorders

... DNA is copied from generation to generation by a process called semiconservative replication This is a highly accurate process Even so, occasionally, a copy error occurs resulting in a mutation Mutations can arise by other processes DNA “code” is transcribed to RNA and then translated into protein s ...
regulation-2013
regulation-2013

... 8-Regulation by protein stability •Ubiquitin-dependent proteolysis. Cyclins control of cell cycle. • Protein molecule is tagged for degradation by attachment of a 20 kDa protein, ubiquitin ...
Chapter 2 PowerPoint Slides
Chapter 2 PowerPoint Slides

... • predict 3D structure from primary amino acid sequence • considered computationally intractable • however, many individuals are working on this problem ...
GMOs – The Hidden Science
GMOs – The Hidden Science

Principles of Biology Lake Tahoe Community College
Principles of Biology Lake Tahoe Community College

... 5. Explain how crossing over can unlink genes. 6. Explain how linkage maps are created; be able to create a linkage map. 7. Define a map unit. 8. Explain why Mendel did not find linkage between seed color and flower color, despite the fact that these genes are on the same chromosome. 9. Explain how ...
Questions to lecture 15. Cancer
Questions to lecture 15. Cancer

Systems Microbiology 1
Systems Microbiology 1

... Genome annotation is the conversion of raw seque3nce data into a list of genes, promoter elements, and regulatory sequences present in the organism. Gene assembly is the ordering of the DNA fragments and eliminating overlaps in the sequence but is not involved in “making sense” of the sequence data. ...
Tutorial_7 (2016) - Gene Expression
Tutorial_7 (2016) - Gene Expression

... The Gene Ontology project provides an ontology of defined terms representing gene product properties. ...
17.2.3 Thought Lab 17.1 Map
17.2.3 Thought Lab 17.1 Map

Document
Document

... after the multiple testing correction. If there are no significant genes left after multiple testing correction, you probably have some differences, but not enough power in your experiment to detect those differences. In that case the top 10 genes are still the ones that are most likely to validate. ...
Chromosome Mapping Lab
Chromosome Mapping Lab

... Chromosomes (cont’d) ...
Genetic Vulnerability Factors - Early Psychosis Intervention
Genetic Vulnerability Factors - Early Psychosis Intervention

... DNA stands for deoxyribonucleic acid. This complicated name just describes the chemical structure of the molecule. DNA is made up 4 types of smaller unit called nucleotides. These 4 types of DNA unit (the nucleotides) each have a letter, they are A C G and T. The DNA in each cell of our bodies conta ...
Abstract Format
Abstract Format

... Department of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel; 2Department of Biological Sciences, Bethlehem University, Bethlehem, Palestinian Authority; 3Department of Otolaryngology, Head & Neck Surgery, Sheba Medical Center, Tel Hasho ...
Identification of fertility genes required for microgametogenesis in
Identification of fertility genes required for microgametogenesis in

... National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China The process of microgametogenesis occurs within the developing pollen. It depends on two rounds of meiosis of microspore, and sporophitic func ...
Part B
Part B

... hybridization and variable hybridization across arrays • For instance, Affymetrix GeneChips incorporate a set of mismatching oligonucleotides for each perfect match set to determine non-specific hybridization • Controls are important where duplicate arrays are being used to study differential gene e ...
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Gene expression profiling



In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
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