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Liu Drosophila Library Overview
Liu Drosophila Library Overview

... to be produced, and proteins to be depleted. Screening can then be performed (e.g. for drug resistance), and sgRNA populations between control and treated populations compared by high throughput sequencing. It is important to maintain coverage of the library throughout the screening process, and ide ...
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III Transcriptional Regulation
III Transcriptional Regulation

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... A person who has one dominant and one recessive allele. They ‘carry’ the gene for a disorder but are not affected themselves. ...
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Lecture 12
Lecture 12

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Large Scale expression Profiling to find transcription

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A Tale of Three Inferences

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Eukaryotic Gene Regulation

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Gene expression profiling



In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
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