Ancient origins: complement in invertebrates
... identity to its human counterpart with homology being concentrated around the functionally critical thiolester group and a number of other regions including the R[RK][RK]R C3α/β excision motif (Fig. 1). After SDSPAGE, SpC3 reduces to an α-chain (130 kDa) and a βchain (80 kDa) that are of equivalent ...
... identity to its human counterpart with homology being concentrated around the functionally critical thiolester group and a number of other regions including the R[RK][RK]R C3α/β excision motif (Fig. 1). After SDSPAGE, SpC3 reduces to an α-chain (130 kDa) and a βchain (80 kDa) that are of equivalent ...
GENOTYPE-PHENOTYPE CORRELATION USING
... Biological science has undergone a revolution in the past few decades. The successes of molecular and structural biology, biochemistry, and genetics have yielded large amounts of data that are increasingly quantitative in nature. This quantitative analysis of this data has attracted the use of techn ...
... Biological science has undergone a revolution in the past few decades. The successes of molecular and structural biology, biochemistry, and genetics have yielded large amounts of data that are increasingly quantitative in nature. This quantitative analysis of this data has attracted the use of techn ...
Pluripotent cells (stem cells) - an der Universität Duisburg
... The cleaving amphibian egg first forms a morula, followed by the blastula stage, when a cavity called the blastocoele develops. At the gastrula stage, a blastopore is formed in the presumptive endoderm. It is through the blastopore that the endoderm, the mesendoderm on the leading edge of the mesode ...
... The cleaving amphibian egg first forms a morula, followed by the blastula stage, when a cavity called the blastocoele develops. At the gastrula stage, a blastopore is formed in the presumptive endoderm. It is through the blastopore that the endoderm, the mesendoderm on the leading edge of the mesode ...
Brassinosteroid-6-Oxidases from Arabidopsis and
... cytochrome P450 enzyme that converts 6-DeoxoCS to CS via 6-OHCS, a step where the early C-6 oxidation and the late C-6 oxidation pathways are connected (Bishop et al., 1999). This step is the furthest downstream step in BL biosynthesis among those known for mutations and enzymes. A defect in the Dwa ...
... cytochrome P450 enzyme that converts 6-DeoxoCS to CS via 6-OHCS, a step where the early C-6 oxidation and the late C-6 oxidation pathways are connected (Bishop et al., 1999). This step is the furthest downstream step in BL biosynthesis among those known for mutations and enzymes. A defect in the Dwa ...
Transcriptional control of glial cell development in Drosophila
... in Biggin and Tjian, 2001). Many fundamental concepts such as cis-regulatory DNA elements acting over long distances, and the regulation of development by hierarchical cascades of transcription factors originated in research on Drosophila. Whether or not homologous mechanisms are controlling glial c ...
... in Biggin and Tjian, 2001). Many fundamental concepts such as cis-regulatory DNA elements acting over long distances, and the regulation of development by hierarchical cascades of transcription factors originated in research on Drosophila. Whether or not homologous mechanisms are controlling glial c ...
A locus for posterior polymorphous corneal dystrophy (PPCD3
... into cells with an epithelial-like appearance [Krachamer, 1985]. The clinical phenotype of PPCD can vary from relatively benign Descemet’s thickening to severe progression towards vision loss from corneal edema [Cibis et al., 1977; Threlkeld et al., 1994; Weisenthal and Streeten, 1997]. In about 40% ...
... into cells with an epithelial-like appearance [Krachamer, 1985]. The clinical phenotype of PPCD can vary from relatively benign Descemet’s thickening to severe progression towards vision loss from corneal edema [Cibis et al., 1977; Threlkeld et al., 1994; Weisenthal and Streeten, 1997]. In about 40% ...
Giant viruses, giant chimeras: The multiple evolutionary histories of
... analyses for each marker. In fact, when independent phylogenetic trees of the proteins used by Raoult et al. were carried out with a rich taxonomic sampling, the results were completely different [18]. Not only HGT events were detected, but Mimivirus did no longer form an independent branch at the b ...
... analyses for each marker. In fact, when independent phylogenetic trees of the proteins used by Raoult et al. were carried out with a rich taxonomic sampling, the results were completely different [18]. Not only HGT events were detected, but Mimivirus did no longer form an independent branch at the b ...
Gene transfer in bacteria - McGraw Hill Higher Education
... • In B. subtilis, competence occurs only in nearly starved cells at specific times in growth culture 1% - 5% of cells become competent ...
... • In B. subtilis, competence occurs only in nearly starved cells at specific times in growth culture 1% - 5% of cells become competent ...
Oakley
... occur by chance rather easily [26]. A second mechanism for the structural replication of eyes could be quantitative effects on a patterning process. In other words, replication can be caused by a periodic processing phenomenon, whereby one process generates an array of structures. The facets of comp ...
... occur by chance rather easily [26]. A second mechanism for the structural replication of eyes could be quantitative effects on a patterning process. In other words, replication can be caused by a periodic processing phenomenon, whereby one process generates an array of structures. The facets of comp ...
Robust gene silencing mediated by antisense small RNAs in the
... exclude the possibility that the given genes have low mRNA levels due to low promoter activity, we determined the activity of the endogenous promoters by creating luciferase reporter gene constructs, and found that all three promoters are active and share sequence identity in both amebic strains (da ...
... exclude the possibility that the given genes have low mRNA levels due to low promoter activity, we determined the activity of the endogenous promoters by creating luciferase reporter gene constructs, and found that all three promoters are active and share sequence identity in both amebic strains (da ...
2D Representation of Transcriptomes by t- SNE Exposes Relatedness between Human Tissues
... The use of dimensionality reduction methods such as multidimensional scaling (MDS) or principal component analysis (PCA) are popular techniques for data representation in low dimensions[1]. As an example, the use of MDS was used in a recent study from the GTEx consortium [1] where transcriptome data ...
... The use of dimensionality reduction methods such as multidimensional scaling (MDS) or principal component analysis (PCA) are popular techniques for data representation in low dimensions[1]. As an example, the use of MDS was used in a recent study from the GTEx consortium [1] where transcriptome data ...
Brooker Chapter 5 - Volunteer State Community College
... “In conversation with Morgan … I suddenly realized that the variations in the length of linkage, already attributed by Morgan to differences in the spatial orientation of the genes, offered the possibility of determining sequences [of different genes] in the linear dimension of the chromosome. I wen ...
... “In conversation with Morgan … I suddenly realized that the variations in the length of linkage, already attributed by Morgan to differences in the spatial orientation of the genes, offered the possibility of determining sequences [of different genes] in the linear dimension of the chromosome. I wen ...
The microRNAs of Caenorhabditis elegans
... eliminated in lin-4 mutant animals. Instead, LIN-14 persisted into adulthood, mirroring the effect of the semidominant mutant and thus demonstrating a key role for lin-4 in downregulating lin-14 at the end of L1 [28]. The major breakthrough, however, came with the cloning of lin-4, and the realizati ...
... eliminated in lin-4 mutant animals. Instead, LIN-14 persisted into adulthood, mirroring the effect of the semidominant mutant and thus demonstrating a key role for lin-4 in downregulating lin-14 at the end of L1 [28]. The major breakthrough, however, came with the cloning of lin-4, and the realizati ...
Evolution of colour vision in primates
... a. Do you think there are many differences between the two sequences? What is the sequence similarity between the two genes? Hint: use the Statistics panel in Geneious to obtain this information (the % tab on the lower right of the screen). b. Which of the differences between the sequences might b ...
... a. Do you think there are many differences between the two sequences? What is the sequence similarity between the two genes? Hint: use the Statistics panel in Geneious to obtain this information (the % tab on the lower right of the screen). b. Which of the differences between the sequences might b ...
Transcript
... [DOEBLEY:] So Neil, the reason I wanted to bring you out here was to show you just how different corn and teosinte are. [LOSIN:] Yeah. [DOEBLEY:] This is a teosinte plant, and it doesn’t look anything like a typical corn plant. [LOSIN:] No. [DOEBLEY:] You can start by just looking at the base, it j ...
... [DOEBLEY:] So Neil, the reason I wanted to bring you out here was to show you just how different corn and teosinte are. [LOSIN:] Yeah. [DOEBLEY:] This is a teosinte plant, and it doesn’t look anything like a typical corn plant. [LOSIN:] No. [DOEBLEY:] You can start by just looking at the base, it j ...
Major influence of repetitive elements on disease
... phenotype-genotype relationships less reliable because the sequence that is deleted or duplicated in each patient is different and the affected region may also involve other genes. This review focused on disease-associated CNVs in order to show that although numerous cases of instability driven by r ...
... phenotype-genotype relationships less reliable because the sequence that is deleted or duplicated in each patient is different and the affected region may also involve other genes. This review focused on disease-associated CNVs in order to show that although numerous cases of instability driven by r ...
Matthew Kwong - GEP Community Server
... three for each isoform, and Genscan predicts seven exons for the feature that aligns with the Blastx result (feature 1). Thus, there is a high possibility that contig 10 contains only part of the ey gene. Further comparison between the genome browser and transcript details indicate that it likely co ...
... three for each isoform, and Genscan predicts seven exons for the feature that aligns with the Blastx result (feature 1). Thus, there is a high possibility that contig 10 contains only part of the ey gene. Further comparison between the genome browser and transcript details indicate that it likely co ...
A natural recessive resistance gene against potato virus
... Based on the hypothesis that the dominant eIF4E allele is required for susceptibility to PVY, we predicted that transient expression of the dominant susceptible eIF4E allele in pepper plants resistant to PVY would lead to PVY genome amplification in the PVX-infected cells expressing the fulllength e ...
... Based on the hypothesis that the dominant eIF4E allele is required for susceptibility to PVY, we predicted that transient expression of the dominant susceptible eIF4E allele in pepper plants resistant to PVY would lead to PVY genome amplification in the PVX-infected cells expressing the fulllength e ...
Selective Crossover in Genetic Algorithms: An Empirical Study
... of 1.0 and recorded the number of evaluations. If the GA did not always find a solution of 1.0, evaluation was terminated at 600 generations. Tests for significance were carried out, to compare the three crossover operators, on the number of evaluations yielded. Using the t-test with a 5% significan ...
... of 1.0 and recorded the number of evaluations. If the GA did not always find a solution of 1.0, evaluation was terminated at 600 generations. Tests for significance were carried out, to compare the three crossover operators, on the number of evaluations yielded. Using the t-test with a 5% significan ...
GSEA - Bioinformatics Unit
... 1. Choose true (default) to have GSEA collapse each probe set in your expression dataset into a single gene vector, which is identified by its HUGO gene symbol. In this case, you are using HUGO gene symbols for the analysis. The gene sets that you use for the analysis must use HUGO gene symbols to i ...
... 1. Choose true (default) to have GSEA collapse each probe set in your expression dataset into a single gene vector, which is identified by its HUGO gene symbol. In this case, you are using HUGO gene symbols for the analysis. The gene sets that you use for the analysis must use HUGO gene symbols to i ...
Biology 423 L Sept. 29/30 Conjugation Lab Hfr Mapping to
... that the recipient strain requires both leucine and proline in the media in order to grow since it contains mutations in the biosynthetic pathways for both of these amino acids. The strain also requires several other amino acids that will be provided in the media. Each of these genes could also be u ...
... that the recipient strain requires both leucine and proline in the media in order to grow since it contains mutations in the biosynthetic pathways for both of these amino acids. The strain also requires several other amino acids that will be provided in the media. Each of these genes could also be u ...
Document
... When alleles are being segregated during gamete formation, does the segregation of one pair alleles have any affect on the segregation of a different pair of alleles? In other words, does the gene that determines if a pea plant is tall or dwarf have any affect on the gene for seed color? ...
... When alleles are being segregated during gamete formation, does the segregation of one pair alleles have any affect on the segregation of a different pair of alleles? In other words, does the gene that determines if a pea plant is tall or dwarf have any affect on the gene for seed color? ...
Belote, J. m., F. M. Hoffmann, M. McKeown, R. Chorsky, and B. S. Baker. (1990). Cytogenetic analysis of chromosome region 73AD of Drosophila melanlgaster. Genetics 125: 783-793.
... lethal mutants,andthree visible mutants were recovered. The 45 X-ray-induced mutants (21 st alleles, 21 non-st lethals, and 3 non-st visibles) were analyzed cytologically to see if they were associated with gross chromosomal rearrangementsin the 73AD region. Of the 21 st mutants, 13 were associated ...
... lethal mutants,andthree visible mutants were recovered. The 45 X-ray-induced mutants (21 st alleles, 21 non-st lethals, and 3 non-st visibles) were analyzed cytologically to see if they were associated with gross chromosomal rearrangementsin the 73AD region. Of the 21 st mutants, 13 were associated ...
Gene expression profiling
In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.