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Gene expression (central dogma)
Gene expression (central dogma)

... A DNA molecule consists of functional units called genes. Each gene provides instructions for a functional product (a molecule needed to perform a job in the cell). In many cases, the functional product of a gene is a protein. The functional products of most known genes are proteins, or, more accura ...
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b - AET

... ranchers have selected plant varieties and livestock for specific traits. Plant breeders select plant varieties which produce more seed or fruit. Livestock producers select animals with specific traits such as increased milk production, ample muscle mass or structural correctness. Selecting for thes ...
Dominance of a non-pathogenic over a pathogenic G protein gene
Dominance of a non-pathogenic over a pathogenic G protein gene

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Bio3124 Lecture 10
Bio3124 Lecture 10

... Small Regulatory RNAs • found in bacterial intergenic regions • Regulate transcription or interfere with translation • antisense nature of sRNA allows its binding to mRNA - ex. pairing of RNAIII with 5’-end of mRNA prevents ribosome assembly, ie. halting translation – mRNA degradation ...
Genetics Unit Organization
Genetics Unit Organization

... is, they are always turned “on,” e.g., the ribosomal genes. 
 In eukaryotes, gene expression is complex and control involves regulatory genes, regulatory elements and transcription factors that act in concert. Examples: o Transcription factors bind to specific DNA sequences and/or other regulatory p ...
Global synthetic-lethality analysis and yeast functional profiling
Global synthetic-lethality analysis and yeast functional profiling

... phenotypes. For example, both synthetic genetic array (SGA) and synthetic-lethality analysis by microarray (SLAM) methods have been used for synthetic-lethality screens. Global analysis of synthetic lethality promises to identify cellular pathways that ‘buffer’ each other biologically. The combinati ...
teachers notes - Learning on the Loop
teachers notes - Learning on the Loop

File - Prader
File - Prader

... Prader-Willi Syndrome (PWS) is a genetic disorder that affects 1 in 15,000 individuals worldwide and is the most common genetic cause of obesity in children. PWS individuals progress through two main stages of symptoms: The first is characterized by decreased muscle tone and the second by insatiable ...
The Genetics of Werewolves - Westminster Public Schools Wiki
The Genetics of Werewolves - Westminster Public Schools Wiki

Leukaemia Section t(5;11)(q35;q12) NSD1/FEN1 Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(5;11)(q35;q12) NSD1/FEN1 Atlas of Genetics and Cytogenetics in Oncology and Haematology

... motifs, 3 nuclear translocation signals, 4 plant homeodomain (PHD) finger regions, and a prolinerich region. The protein acts as a basic transcriptional factor and also as a bifunctional transcriptional regulator, capable of both negatively or positively influencing transcription, depending on the c ...
Control of Gene Expression
Control of Gene Expression

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4_Hereditary Disorders - V14-Study

...  Females may be either heterozygous or homozygous for mutant gene (b/c have two X chromosomes)  Disorder may demonstrate either recessive or dominant expression  Males will be affected if inherit gene, regardless of dominance (b/c have only one X chromosome)  Characteristics of X-linked recessiv ...
Genetics Practice Questions
Genetics Practice Questions

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... and novel labeling strategies with life science applications in research, drug discovery and ...
Identifying Genes in E. coli
Identifying Genes in E. coli

... PMO have been found  Mutants picked are not revertants back to the non-leaky E. coli strain  Mutations have not been in the target region of the PMO  Library experiment did not result in finding ...
Introduction to Genetics
Introduction to Genetics

... Infant lacks an enzyme to process the amino acid phenylalanine which can build up and poison the nervous system. Severe, irreversible brain damage unless baby is fed a special diet low in phenylalanine the first month. (Nature & Nurture) ...
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I A

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Lectures for December 5&7, 2005 (Chapter 18: The Genetic Basis of
Lectures for December 5&7, 2005 (Chapter 18: The Genetic Basis of

... the same trait. The F2 phenotypic ratio is ...
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ppt - Chair of Computational Biology

... phosphorylation-regulated manner where they interact with the CLOCK–BMAL1 complex to repress their own activator. At the end of the circadian cycle, the PER and CRY proteins are degraded in a CKI-dependent manner, which releases the repression of the transcription and allows the next cycle to start. ...
Some - Laker Science
Some - Laker Science

... Explain the three major experimental contributions (Griffiths, Avery, and Hershey/Chase) that gave evidence for DNA as the hereditary molecule. Describe the discovery of and structure of DNA including the contributions of Erwin Chargaff, Rosalind Franklin, Francis Crick and James Watson. Described h ...
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... Remark about the multiplicity problem Having many genes has advantages and disadvantages. disadvantage: multiple testing problem. There is a big chance of false positives. Bonferoni-type corrections could be far too conservative and too restrictive. advantage: all genes give similar data. Informati ...
Transgenic Animals and Plants
Transgenic Animals and Plants

... Transgenic -> stable introduction of a gene into another organism -> For Unicellular organisms (such as bacteria or yeast) all transformed cells are -> transgenic -> For multicellular organisms (such as animals, plants,..) ...
Expression analysis of a chicory fructan 1‐exohydrolase gene
Expression analysis of a chicory fructan 1‐exohydrolase gene

... genes during cold induction and proposed a physiological role for the deletion of the b-fructofuranosidase DPN motif. Interestingly, since fructans are proposed to be localized in the vacuoles, it might be expected that plant 1FEH cDNAs would be more closely related to the vacuolar invertases; howev ...
benzer 15 kb benzer
benzer 15 kb benzer

... distance he found was 0.01%. Benzer concluded this must be the distance between adjacent mutations, this is actually the distance between base pairs in DNA. The genetic mapping of the rII region was a leap forward in our understanding of genetics, however the problem still remained that it was not ...
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Gene expression profiling



In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
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