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DIHYBRID CROSSES
DIHYBRID CROSSES

...  what is the likelihood a specific trait will by inherited in regards to both phenotype and genotype  the number of ways a specific event can occur (total number of possible genetic outcomes) Rules: 1. past outcomes have no effect on the future outcomes 2. the probability of independent events occ ...
GO2PUB - GenOuest
GO2PUB - GenOuest

... GO2PUB to answer this demand in the field of the genetics. Our purpose was to use the knowledge within the Gene Ontology (GO) to build PubMed queries and display results for a quick access to the information. GO annotations link genes products to the metabolic pathways in which these are involved. G ...
Transcription & Translation
Transcription & Translation

... • Associated with a gene(s) is an up-gene promoter/operator sequence for RNA polymerase binding and a down-gene termination sequence. • Gene transcription can be regulated (on/off switch) negatively or positively by regulatory proteins (more later). ...
Epigenetics
Epigenetics

... • genes are “turned on” or “turned off” by chemical alterations to: - DNA (e.g.methylation) or RNA - histone modification (methylation, acetylation, etc.) • epigenetic changes can be heritable • if DNA (genome) is the hardware of a computer, epigenetics (epigenome) is like the “software”. ...
Basic Genetics Concepts
Basic Genetics Concepts

... several or many genes. Some genes can tolerate aneuploidy, while others are very sensitive to it. • The chromosome breaks themselves can cause genetic harm, if they break a gene in half. Otherwise, any genetic harm is due to aneuploidy. • Most of the time, a person is heterozygous for the unusual ch ...
Forward Genetic Screens: Strategies and challenges
Forward Genetic Screens: Strategies and challenges

... Every integration results in silencing Cons: Mutagenesis rate is lower than ENU Mutagenesis is very labor intensive Slight bias towards open regions of the genome (higher insertion  rate at 5’ ends) ...
Chapter 7: Genetics Lesson 7.3: Human Genetics and Biotechnology
Chapter 7: Genetics Lesson 7.3: Human Genetics and Biotechnology

... The remaining pair of human chromosomes consists of the sex chromosomes, X and Y. Females have two X chromosomes, and males have one X and one Y chromosome. In females, one of the X chromosomes in each cell is inactivated and known as a Barr body. This ensures that females, like males, have only one ...
Non-coding RNA for ZM401, a Pollen
Non-coding RNA for ZM401, a Pollen

... suggested that they function without being translated into proteins. Some genes encode RNAs, rather than proteins, as their final products. tRNA, rRNA, and the small nuclear RNAs and nucleolar RNAs have been studied extensively, and were relatively straightforward to identify by homology searches or ...
Lecture 14 - The Chromosomal Basis of Inheritance
Lecture 14 - The Chromosomal Basis of Inheritance

... • A linkage map shows the positions of known genes relative to each other in terms of recombination frequency – The greater the frequency of recombination between two genes, the farther apart they are ...
Chapter 14 Mendelian Genetics Notes
Chapter 14 Mendelian Genetics Notes

... Genetics—the scientific study of inheritance ...
BMC Genomics Expansion of the Bactericidal/Permeability Increasing-like (BPI-like) protein locus in cattle
BMC Genomics Expansion of the Bactericidal/Permeability Increasing-like (BPI-like) protein locus in cattle

... humans and mice, which are related to BPI through sequence similarity, exon segmentation and predicted secondary structure [10,11]. All but two of these are found as a gene cluster at a single locus on human chromosome 20 or the syntenic region of mouse chromosome 2. The similarity of the products o ...
BRCA1 and BRCA2 for men - Oxford University Hospitals
BRCA1 and BRCA2 for men - Oxford University Hospitals

... it gives more information for women. As their risk of developing cancer below the age of 30 is low , we would not usually begin any screening for them until at least this age. Some men choose to delay testing until their children are in their 20’s, when it is more relevant for them. It can be diffic ...
Children`s health and parents related by blood
Children`s health and parents related by blood

... Genetic disorders are found in every community. However, communities where cousin marriage is common have more children with recessive disorders. In the UK, the Pakistani origin community has the highest level of cousin marriage, so a higher proportion of children in this community have recessive di ...
Application of Molecular Biotechnologies to Remediation
Application of Molecular Biotechnologies to Remediation

... Need to optimize the gradient and electrophoresis duration DNA fragment < 500bp Need large quantity of DNA ...
Retroviruses as Gene Therapy Vectors
Retroviruses as Gene Therapy Vectors

... Overview of Today’s Presentation ...
Chapter 13
Chapter 13

...  iii. Both enzymes are present in an IA/IB individual, and some H antigens will be modified to the A antigen while others are modified to the B antigen.  iv. Neither enzyme is present in an i/i individual and so the H antigen remains unmodified. ...
Population Genetics Worksheet
Population Genetics Worksheet

... 1. In most populations, the frequency of two alleles is calculated from the proportion of homozygous recessives (q2), since it is the only identifiable genotype directly from its phenotype. If only the dominant phenotype is known, q2 may be calculated (1-frequency of dominant phenotype) . 2. All cal ...
Gene Transfer in Bacteria/Phage
Gene Transfer in Bacteria/Phage

... Cotransduction frequency = tendency for genes to be transferred together on same piece of transducing DNA ...
Review and Non-Mendelian Genetics
Review and Non-Mendelian Genetics

... and the other has green pods. Yellow is dominant to green. Parent plant genotypes ____ X ____ Draw Punnett square. What phenotypic results will the student find in the F1 generation? ...
Introduction of Microarray - genomics-lab
Introduction of Microarray - genomics-lab

... finding and refining biological pathways Mutation and polymorphism detection ...
139 chapter 10 PPT with captions for visual
139 chapter 10 PPT with captions for visual

... together vs. MZ twins raised apart find that the identical twins raised apart are quite similar to each other, as much as identical twins raised together, suggesting a strong genetic influence on personality. In response, some critics suggest that identical twins may experience more “shared environm ...
Statistical analysis of DNA microarray data
Statistical analysis of DNA microarray data

... photodetector voltage, imbalance in total amount of RNA in each sample, difference in uptaking of the dyes, etc. • The objective is is to adjust the gene expression values of all genes so that the ones that are not really differentially expressed have similar values across the array(s). ...
Identification of an antibacterial protein by functional screening of a
Identification of an antibacterial protein by functional screening of a

... antimicrobial producers is hindered by the fact that not all are cultivable in the laboratory. Metagenomics allows access to the genetic potential of whole microbial communities in an environmental sample (Mullany, 2014) and has allowed the isolation of novel antimicrobial products, for example, inv ...
Biological Explanations powerpoint
Biological Explanations powerpoint

... • Some genes always lead to certain characteristics; these are known as dominant genes. To produce a characteristic, dominant genes need to be on only one pair of chromosomes (one copy). • Some genes need more than one copy to produce a characteristic, there are known as recessive genes – if a Rece ...
The Comparison of Transcriptomes Undergoing Waterlogging at the
The Comparison of Transcriptomes Undergoing Waterlogging at the

... facilitation (51.7%), universal stress related (73.2%), and unknown function (55.2%). A total of 1438 of the regulated genes were specifically regulated in ZS9, with 612 being up-regulated and 826 genes down-regulated in this tolerant variety (Appendix E). The GO term analysis (Table 3) showed that ...
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Gene expression profiling



In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
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