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Characterization and determination of holin protein of Streptococcus suis bacteriophage SMP
Characterization and determination of holin protein of Streptococcus suis bacteriophage SMP

... identified upstream of the first start codon (Figure 1B). HolSMP consists of 142 amino acids (15.7 kDa) and shows 88% similarity to the sequence of a putative holin of Streptococcus phage MM1 (accession number NP 150180), and exhibits the characteristics of phageholin_4 superfamily. The results of P ...
Homogenisation in the ribosomal RNA genes of an Epichloe
Homogenisation in the ribosomal RNA genes of an Epichloe

... Finally, evidence is presented that suggests the rate of ...
Microbial Genomes - Griffith University
Microbial Genomes - Griffith University

... sequences (genes) in a genome sequence is known as annotation • although an annotated genome sequence provides a large amount of important information it is still merely a starting point for completely ...
Mendelian Genetics
Mendelian Genetics

... peas led to the discovery of the basic principles of ...
2009 Hart and Grosbe.. - Evolution and Ecology | UC Davis
2009 Hart and Grosbe.. - Evolution and Ecology | UC Davis

... Holometabolous Insect Genomes Do Not Contain an Onychophoran-Like Genome Acquired by Hybridization Evolutionary biologists have often (and sometimes loudly) debated whether major evolutionary innovations in phenotypes are driven mainly by the evolution of regulatory sequences that modify the functi ...
PGLO Transformation LAB AP LAB 7
PGLO Transformation LAB AP LAB 7

... Source of “glowing gene” for this experiment ...
PPT - Bioinformatics.ca
PPT - Bioinformatics.ca

... Variant vs Gene Information We have to consider information at two levels: • Gene – Is the gene central to processes related to cancer? (e.g. proliferation, apoptosis, matrix degradation) – Is the gene sensitive to perturbation? (e.g. haploinsufficiency) ...
Slide 1
Slide 1

... Cell cycle mutations may lead to cancer ...
Introduction - people.bath.ac.uk
Introduction - people.bath.ac.uk

... al., 2006). These indicate an evolutionarily conserved function for PtdIns(3,5)P2 in the endo-lyosomal pathway. Compartmental enlargements of lysosomes in lower eukaryotes however occur later in the endosomal pathway than those of Drosophila and mammals where defects occur at the level of early endo ...
Bioreporters in microbial ecology Johan HJ Leveau*‡ and Steven E
Bioreporters in microbial ecology Johan HJ Leveau*‡ and Steven E

... function properly is illustrated in Figure 2. Such sites must exist, at least in habitats for which it has been observed that a substantial fraction of bioreporter cells is metabolically challenged and, as such, may not be able to report. As Figure 2 clearly shows, different estimates of habitat var ...
mendel intro
mendel intro

... pairs every time you want to mention the allele, so we shorten it.  We’ll usually use one letter for each gene, like the letter R. Then, the different alleles are represented by the R being either upper-case R or lowercase r. ...
Centipede Hox genes - Development
Centipede Hox genes - Development

... condition maintained in the centipede. Last, we found that Hox expression correlates with tagmatic boundaries, consistent with the theory that changes in Hox genes had a major role in evolution of the arthropod body plan. ...
Gene Section CDX2 (caudal-related homeobox 2) Atlas of Genetics and Cytogenetics
Gene Section CDX2 (caudal-related homeobox 2) Atlas of Genetics and Cytogenetics

... Abnormal protein Fusion of ETV6 exon 2 to CDX2 exon 2. The predicted protein contains the N-terminal region of ETV6 38 fused to the entire homeobox of CDX2. The single case described that harbours this fusion also expressed normal CDX2, which is not normally expressed in haemopoietic cells. ...
Overview of Recombinant DNA Experiments Covered by
Overview of Recombinant DNA Experiments Covered by

... The deliberate transfer of recombinant DNA, or DNA or RNA derived from recombinant DNA, into one or more human research participants are subject to the NIH Guidelines. This includes the transfer of DNA with defective viral vectors, such as retroviral, adenoviral and lentiviral vectors, along with th ...
Eds., N. Hamamura, S. Suzuki, S. Mendo, C. M. Barroso,... © by TERRAPUB, 2010.
Eds., N. Hamamura, S. Suzuki, S. Mendo, C. M. Barroso,... © by TERRAPUB, 2010.

... Abstract—Mercury resistance is the only bacterial metal resistance system whose mechanism leads to a delicate transformation of its toxic target. The mechanism involves the reduction of the highly reactive cationic form of mercury into metallic vapor. The genes, which are responsible for this resist ...
Biology 22 Problem Set 1 Spring 2003
Biology 22 Problem Set 1 Spring 2003

... What are the genotypes of the original parents in this cross? Write each genotype to show which alleles are linked together on the same chromosome. What are the genotypes of the F1 males and females? Write each genotype to show which alleles are linked together on the same chromosome. Draw a genetic ...
SGD: Saccharomyces Genome Database.
SGD: Saccharomyces Genome Database.

... ORFs identified by the systematic sequencing project, the hypothetical translation of all ORF sequences, and the non-ORF DNA sequences. In addition to the protein sequence datasets, SGD also includes the topic category Protein_Info which contains information curated by the YPD (27,28) resource. Patt ...
Contemporary, yeast-based approaches to
Contemporary, yeast-based approaches to

... can reveal the function of the human ortholog. Epistatic interactions can be explored by examining the consequences of multiple deletions. Adding or removing adjacent chromosome segments can reveal the consequences of more complex changes in copy number. (b) An alignment between a human gene (light ...
P.abyssi PDF version
P.abyssi PDF version

... The complete genome of P. abyssi GE5 (deposited at the CNCM under strain Orsay) has been sequenced at Genoscope in 1998 and released before publication (http:/ /www.genoscope.cns.fr/Pab/). This sequence has been instrumental in the first experimental identification of a replication origin in Archaea ...
Plastid endosymbiosis, genome evolution and the origin of green
Plastid endosymbiosis, genome evolution and the origin of green

... this, however, might be the tip of an iceberg. Fine-scale analyses of the Arabidopsis genome led to the discovery that, despite its highly reduced state, the remnant plastid genome continues to pepper the nucleus with sequences of cyanobacterial ancestry, so called NUPTs (nuclear plastid DNA) [39,40 ...
Creating a Venn diagram and list for unique genes from RAST
Creating a Venn diagram and list for unique genes from RAST

... Go to rast.nmpdr.org  Login to RAST (username: newmanlab  password: 16srrna1)  In the Jobs Overview window, find the organism you wish to focus on by searching the Name  column and click View Details under Annotation Progress  In the Job Details window, click Browse annotated genome in the SEED View ...
Exercise 4.4 Designing a genetic screen
Exercise 4.4 Designing a genetic screen

... methods have been introduced that allow direct comparison of protein abundance from two conditions that are separated on the same gel (von Eggeling et al, 2001). This approch is also known as 2DDifference Gel- Electrophoresis (DIGE). Several other methods available for protein separation offer dist ...
Chapter 14 - Speedway High School
Chapter 14 - Speedway High School

... i born b with ith extra t fi fingers or ttoes – this unusual trait is dominant • Known as polydactyly • Natural Selection at work! ...
Summarizer PowerPoint - Butler Biology
Summarizer PowerPoint - Butler Biology

Franks et al 2016 Mol Ecol - Department of Ecology and Evolution
Franks et al 2016 Mol Ecol - Department of Ecology and Evolution

... Nine of our ten chosen SNPs amplified consistently and were used for the validation analysis. We found high concordance in allele frequencies obtained from KASP genotyping compared to pooled Illumina sequencing (Pearson correlation r = 0.83, P < 0.001; Fig. S1, Supporting information), indicating th ...
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Gene expression profiling



In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
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