Analysis continued Each TopHat run will result in four files: a list of
... an exon that was predicted by the output from CuffLinks was actually seen in the reference. The Sn and Sp columns calculate sensitivity (the proportion of exons, for example, that have been correctly identified) and specificity (the proportion of predicted exons that are annotated as such in the ref ...
... an exon that was predicted by the output from CuffLinks was actually seen in the reference. The Sn and Sp columns calculate sensitivity (the proportion of exons, for example, that have been correctly identified) and specificity (the proportion of predicted exons that are annotated as such in the ref ...
Replication studies in longevity: puzzling findings in Danish
... the gene pools at a young age was lost under survival selection and increasing mortality. Indeed the Italian gene pool seemed to converge with aging towards the values of the Danish gene pool (Table 2). These results may be caused by a different recruitment of Danish and Italian samples, or occur by ...
... the gene pools at a young age was lost under survival selection and increasing mortality. Indeed the Italian gene pool seemed to converge with aging towards the values of the Danish gene pool (Table 2). These results may be caused by a different recruitment of Danish and Italian samples, or occur by ...
American Heart Association Research Proposal, Example
... functionality of deleting suppressor homologs in mammalian cells. Modulation of channel activity will be measured by introducing siRNAs against the suppressor into a TRPV1expressing stable HEK293 cell line and subjecting the cells to electrophysiological and calcium imaging analysis. Changes in the ...
... functionality of deleting suppressor homologs in mammalian cells. Modulation of channel activity will be measured by introducing siRNAs against the suppressor into a TRPV1expressing stable HEK293 cell line and subjecting the cells to electrophysiological and calcium imaging analysis. Changes in the ...
based methods in the environment and hospital patients
... Molecular methods e.g. Gene probing, DNA sequencing of rRNA genes, FISH and PCR have all been used- but may not differentiate between MSSA and MRSA. Other methods include Real-time Q-PCR and MLST. ...
... Molecular methods e.g. Gene probing, DNA sequencing of rRNA genes, FISH and PCR have all been used- but may not differentiate between MSSA and MRSA. Other methods include Real-time Q-PCR and MLST. ...
Notifiable Low Risk Dealing (NLRD)
... Address possible risks to the health and safety of people even if they are considered to be minimal. Include information about how these risks will be managed. What are the risks to the environment from the proposed dealing(s)? Address possible risks to the environment even if they are considered to ...
... Address possible risks to the health and safety of people even if they are considered to be minimal. Include information about how these risks will be managed. What are the risks to the environment from the proposed dealing(s)? Address possible risks to the environment even if they are considered to ...
Chapter 6 GENETIC LINKAGE AND MAPPING IN EUKARYOTES
... the distance between two genes. If two genes are far apart from each other, crossing over is more likely to occur between them compared to two genes that are close together. Figure 6.5 illustrates the possible events that occurred in the F1 female flies of Morgan’s experiment. One of the X chromosom ...
... the distance between two genes. If two genes are far apart from each other, crossing over is more likely to occur between them compared to two genes that are close together. Figure 6.5 illustrates the possible events that occurred in the F1 female flies of Morgan’s experiment. One of the X chromosom ...
Analysis of imputed rare variants
... • Use GRANVIL to test for association of T1D with imputed rare variants within genes across the MHC, using data from the WTCCC. • Investigate the impact on results of: • the MAF threshold for inclusion of rare variants in the analysis; • filtering rare variants on the basis of annotation; ...
... • Use GRANVIL to test for association of T1D with imputed rare variants within genes across the MHC, using data from the WTCCC. • Investigate the impact on results of: • the MAF threshold for inclusion of rare variants in the analysis; • filtering rare variants on the basis of annotation; ...
Mary Ann Osley*, Alastair Fleming, and Cheng
... linkage between ubiquitin and the target protein or between individual ubiquitin molecules in the case of polyubiquitylation. The large family of Ubps suggests that, like E3s, Ubps have significant specificity with respect their targets. Moreover, multiple Ubps may act on a single target. For exampl ...
... linkage between ubiquitin and the target protein or between individual ubiquitin molecules in the case of polyubiquitylation. The large family of Ubps suggests that, like E3s, Ubps have significant specificity with respect their targets. Moreover, multiple Ubps may act on a single target. For exampl ...
Myb genes enhance tobacco trichome production
... phenotype in Nicotiana tabacum. The data presented herein are in good agreement with their results which indicate that the transgene can also regulate trichome differentiation in tobacco. Suppression of the pleiotropic Arabidopsis ttg1-1 mutant by overexpression of the R gene from maize, a monocot, ...
... phenotype in Nicotiana tabacum. The data presented herein are in good agreement with their results which indicate that the transgene can also regulate trichome differentiation in tobacco. Suppression of the pleiotropic Arabidopsis ttg1-1 mutant by overexpression of the R gene from maize, a monocot, ...
A mosaic infertile case of isodicentricY
... babies have normal testes with reduced fertility in the future [6]. Surgery may be performed for from minör genitalia abnormalities such as curved penis, hipospadias, crypthoorchidism to internal female tractus. However even so, internal female tract surgery is optional by virtue of they never cause ...
... babies have normal testes with reduced fertility in the future [6]. Surgery may be performed for from minör genitalia abnormalities such as curved penis, hipospadias, crypthoorchidism to internal female tractus. However even so, internal female tract surgery is optional by virtue of they never cause ...
Determinism
... • Epigenetics - the study of heritable changes in phenotype or gene expression caused by mechanisms other than changes in the underlying DNA sequence. • Example – Cell division of a fertilized egg cell. The egg cell changes into different types of cells (muscle cells, blood cells, etc.) by activatin ...
... • Epigenetics - the study of heritable changes in phenotype or gene expression caused by mechanisms other than changes in the underlying DNA sequence. • Example – Cell division of a fertilized egg cell. The egg cell changes into different types of cells (muscle cells, blood cells, etc.) by activatin ...
Understanding Patterns of Inheritance Through Pedigree
... 3. Geneticist-Educator Network of Alliances (GENA). Overview: This lesson was designed to help high school biology students understand and recognize patterns of inheritance as illustrated by pedigrees. The usual sequence for determining patterns of inheritance in high school text books is to teach r ...
... 3. Geneticist-Educator Network of Alliances (GENA). Overview: This lesson was designed to help high school biology students understand and recognize patterns of inheritance as illustrated by pedigrees. The usual sequence for determining patterns of inheritance in high school text books is to teach r ...
21. Toshihiko Okamotofi`1 Yo Isogai,$2 and T6111 K0izumi*1 :
... N assimilation machinery of maize to determine whether this response to low N also required an adaptation of these processes. We measured the tissue levels of amino acids and quantified the activity of the central N assimilation enzymes across the entire lifecycle of Gaspe maize. Further, we analyse ...
... N assimilation machinery of maize to determine whether this response to low N also required an adaptation of these processes. We measured the tissue levels of amino acids and quantified the activity of the central N assimilation enzymes across the entire lifecycle of Gaspe maize. Further, we analyse ...
c-fos Protein Can Induce Cellular Transformation: A Novel
... 1) only chimeric molecules utilizing the Nco I site could be constructed. However, to be consistent we retained the three-letter code to describe these molecules as if there was a Sal I cleavage site. As previously noted, HHH did not transform cells. HVV, however, did transform cells, showing that t ...
... 1) only chimeric molecules utilizing the Nco I site could be constructed. However, to be consistent we retained the three-letter code to describe these molecules as if there was a Sal I cleavage site. As previously noted, HHH did not transform cells. HVV, however, did transform cells, showing that t ...
The Drosophila serum response factor homolog is required for formation of intervein tissue of the wing and is allelic to blistered. Development 122, 2589-2597. pdf
... and Cohen, 1993). The wing pouch is the oval domain that will give rise to the adult wing blade. The hinge is the proximal structures that fasten the wing blade to the body wall (Ng et al., 1995). (B) DSRF expression detected in a wing disc with a DSRF antiserum (brown peroxidase reaction product). ...
... and Cohen, 1993). The wing pouch is the oval domain that will give rise to the adult wing blade. The hinge is the proximal structures that fasten the wing blade to the body wall (Ng et al., 1995). (B) DSRF expression detected in a wing disc with a DSRF antiserum (brown peroxidase reaction product). ...
Title goes here
... What are hypothesised in the Robot Scientist, and in most of molecular biology, are factual relationships between objects, e.g. the gene ypr060c codes for enzyme chorismate mutase, gene ypr060c exists at location 675628- 674858 (C) on chromosome 16, etc. ...
... What are hypothesised in the Robot Scientist, and in most of molecular biology, are factual relationships between objects, e.g. the gene ypr060c codes for enzyme chorismate mutase, gene ypr060c exists at location 675628- 674858 (C) on chromosome 16, etc. ...
Fetal Hemoglobin Levels in Sickle Cell Disease and
... and, in the case of SS disease, the preferential survival in the peripheral circulation of F cells compared with RBCs containing no detectable Hb F.9 In SS individuals, the variation in F-cell production as measured by the percent F reticulocytes is the major variable contributing to differences in ...
... and, in the case of SS disease, the preferential survival in the peripheral circulation of F cells compared with RBCs containing no detectable Hb F.9 In SS individuals, the variation in F-cell production as measured by the percent F reticulocytes is the major variable contributing to differences in ...
In Silico method for identification of MHC class I
... complex, class I‐related MR1”. All these BLAST pairs cluster with the human MR1 gene, forming an interspecies groups distinct from CD1 (Figure 4, Table S2). Our MR1 results show that this in silico prediction method can also be used for MHC class I‐like molecules oth ...
... complex, class I‐related MR1”. All these BLAST pairs cluster with the human MR1 gene, forming an interspecies groups distinct from CD1 (Figure 4, Table S2). Our MR1 results show that this in silico prediction method can also be used for MHC class I‐like molecules oth ...
Polygenic Disorders
... Estimates the proportion of the phenotypic variation in a population due to genetic differences Heritability asks how much genetics are playing a role in differences in height between people. This is not the same as asking how much genetics usually cause height in any one person. 4. Polygenic and Mu ...
... Estimates the proportion of the phenotypic variation in a population due to genetic differences Heritability asks how much genetics are playing a role in differences in height between people. This is not the same as asking how much genetics usually cause height in any one person. 4. Polygenic and Mu ...
Silene sex chromosome genetic map, p. 1 Expansion of
... Silene sex chromosome genetic map, p. 6 Young sex chromosome systems, in which evolutionary strata are still evolving, are particularly relevant for testing the SA polymorphism hypothesis, because (unlike some ancient sex chromosome systems) they may often have physically large PAR regions, contain ...
... Silene sex chromosome genetic map, p. 6 Young sex chromosome systems, in which evolutionary strata are still evolving, are particularly relevant for testing the SA polymorphism hypothesis, because (unlike some ancient sex chromosome systems) they may often have physically large PAR regions, contain ...
Allele Mining Strategies: Principles and Utilisation for Blast
... regions of the genome. Mutations may take the form of insertions or deletions, which might be together called InDels, or may alter a single nucleotide inside the genome sequence, resulting in a single-nucleotide polymorphism (SNP). The mutations in coding regions could have an excellent effect on th ...
... regions of the genome. Mutations may take the form of insertions or deletions, which might be together called InDels, or may alter a single nucleotide inside the genome sequence, resulting in a single-nucleotide polymorphism (SNP). The mutations in coding regions could have an excellent effect on th ...
Käfer, E. and D. Luk
... Results for UV, mitomycin C (MC) and HIS are summarized in Table 1. All new mutants were found to be cross-sensitive to some mutagenic agents and are, therefore, likely to be defective in DNA repair rather than uptake or metabolism of MMS. Most of the mutants listed in Tables 1 and 2 have been induc ...
... Results for UV, mitomycin C (MC) and HIS are summarized in Table 1. All new mutants were found to be cross-sensitive to some mutagenic agents and are, therefore, likely to be defective in DNA repair rather than uptake or metabolism of MMS. Most of the mutants listed in Tables 1 and 2 have been induc ...
Close - Journal of Integrated OMICS
... Enterococci were obtained from 96 (98%) of the 98 samples analysed. E. hirae was the most prevalent detected species (35 isolates), followed by E. faecalis (30 isolates), E. faecium (27 isolates) and E. durans (4 isolates). E. coli isolates were detected in 90 (92%) of the 98 Iberian lynx faecal sam ...
... Enterococci were obtained from 96 (98%) of the 98 samples analysed. E. hirae was the most prevalent detected species (35 isolates), followed by E. faecalis (30 isolates), E. faecium (27 isolates) and E. durans (4 isolates). E. coli isolates were detected in 90 (92%) of the 98 Iberian lynx faecal sam ...
Gene expression profiling
In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.