Appendix: Fusion Gene Plasmid Construction
... containing promoter sequence from -911 to + 3, in the pCAT(An) expression vector, has been previously described (3). This plasmid was digested with BamH I and Bgl II to remove the IGRP promoter sequence between -911 and -508. A fragment of the IGRP gene promoter from -1342 to -508 was isolated from ...
... containing promoter sequence from -911 to + 3, in the pCAT(An) expression vector, has been previously described (3). This plasmid was digested with BamH I and Bgl II to remove the IGRP promoter sequence between -911 and -508. A fragment of the IGRP gene promoter from -1342 to -508 was isolated from ...
Functional analysis of the regulatory region of a zein gene in
... The transcription of zein genes in maize is tissue-specific and developmentally regulated. The 5' regulatory region of many zein genes contains two promoters, P1 and P2, lying approximately 1000 bases apart. The promoter/enhancer activity of various fragments of the two promoter regions of the zein ...
... The transcription of zein genes in maize is tissue-specific and developmentally regulated. The 5' regulatory region of many zein genes contains two promoters, P1 and P2, lying approximately 1000 bases apart. The promoter/enhancer activity of various fragments of the two promoter regions of the zein ...
Features and phylogeny of the six compared Plasmodium genomes
... [PlasmoDB, news item from Feb 1, 2008]. Indeed, the first in-depth sequencing-based analysis of the P. falciparum transcriptome using high-throughput sequencing (RNA-Seq) led to the revision of only one out of ten gene models and could not find evidence for new protein-coding genes [5]. Thus, P. fal ...
... [PlasmoDB, news item from Feb 1, 2008]. Indeed, the first in-depth sequencing-based analysis of the P. falciparum transcriptome using high-throughput sequencing (RNA-Seq) led to the revision of only one out of ten gene models and could not find evidence for new protein-coding genes [5]. Thus, P. fal ...
DNA Part II Lab
... How does the manipulation of nucleic acids through genetic engineering alter the function of proteins and subsequent cellular processes? How does the sequence of nucleotides in DNA code for a specific sequence of amino acids in a protein? How does the specific sequence of amino acids in a protein de ...
... How does the manipulation of nucleic acids through genetic engineering alter the function of proteins and subsequent cellular processes? How does the sequence of nucleotides in DNA code for a specific sequence of amino acids in a protein? How does the specific sequence of amino acids in a protein de ...
dragon genetics lab
... BACKGROUND In this activity you will study Mendelian genetics. You will work in pairs to produce a dragon from the random mixing of genetic traits. You will notice that in some of the traits one allele is not completely dominant over the other. When an offspring has a phenotype that is an intermedia ...
... BACKGROUND In this activity you will study Mendelian genetics. You will work in pairs to produce a dragon from the random mixing of genetic traits. You will notice that in some of the traits one allele is not completely dominant over the other. When an offspring has a phenotype that is an intermedia ...
rna interference
... appears that the machinery, once it finds a double-stranded RNA molecule, cuts it up, separates the two strands, and then proceeds to destroy other single-stranded RNA molecules that are complementary to one of those segments. dsRNAs direct the creation of small interfering RNAs (siRNAs*) which targ ...
... appears that the machinery, once it finds a double-stranded RNA molecule, cuts it up, separates the two strands, and then proceeds to destroy other single-stranded RNA molecules that are complementary to one of those segments. dsRNAs direct the creation of small interfering RNAs (siRNAs*) which targ ...
Unit 8a-Classical Genetics
... trait? Covered up by dominant purple trait How do we know? it reappears in the next generation ...
... trait? Covered up by dominant purple trait How do we know? it reappears in the next generation ...
here - PHI-base
... Reduced virulence – the transgenic strain still causes some disease formation but this is less than the wild-type strain (ie. a quantitative effect). Synonymous with the term reduced aggressiveness. Unaffected pathogenicity - the transgenic strain which expresses no or reduced levels of a specific g ...
... Reduced virulence – the transgenic strain still causes some disease formation but this is less than the wild-type strain (ie. a quantitative effect). Synonymous with the term reduced aggressiveness. Unaffected pathogenicity - the transgenic strain which expresses no or reduced levels of a specific g ...
human genetic disease - hrsbstaff.ednet.ns.ca
... Pedigree of a family with a history of achondroplasia, an autosomal dominantly inherited disease. …Encyclopædia Britannica, Inc. illustrates the pedigree for a family with achondroplasia, an autosomal dominant disorder characterized by short-limbed dwarfism that results from a specific mutation in t ...
... Pedigree of a family with a history of achondroplasia, an autosomal dominantly inherited disease. …Encyclopædia Britannica, Inc. illustrates the pedigree for a family with achondroplasia, an autosomal dominant disorder characterized by short-limbed dwarfism that results from a specific mutation in t ...
"What is a gene, in the post-ENCODE era?"
... This project represents a major milestone in the characterization of the human genome, and the current findings show a striking picture of complex molecular activity. While the landmark human genome sequencing surprised many with the small number (relative to simpler organisms) of protein-coding gen ...
... This project represents a major milestone in the characterization of the human genome, and the current findings show a striking picture of complex molecular activity. While the landmark human genome sequencing surprised many with the small number (relative to simpler organisms) of protein-coding gen ...
Biol120 Mock Final Examination
... 44. Which of the following statements describes a strand of eukaryotic mRNA? a) It must be spliced to cut out exons and seal together translatable introns b) It is transcribed by RNA polymerase prior to export from the nucleus, and translation by a ribosome c) It has a guanine cap on its 3’ end and ...
... 44. Which of the following statements describes a strand of eukaryotic mRNA? a) It must be spliced to cut out exons and seal together translatable introns b) It is transcribed by RNA polymerase prior to export from the nucleus, and translation by a ribosome c) It has a guanine cap on its 3’ end and ...
dragon genetics lab - Holy Trinity Academy
... a no divorce classroom. The lab must be completed on time. 2. Each partner must pick up five Popsicle sticks -- one of each color of autosome, and one sex chromosome stick. Each side of a stick represents a chromosome, and the two sides together represent a pair of homologous chromosomes. 3. For eac ...
... a no divorce classroom. The lab must be completed on time. 2. Each partner must pick up five Popsicle sticks -- one of each color of autosome, and one sex chromosome stick. Each side of a stick represents a chromosome, and the two sides together represent a pair of homologous chromosomes. 3. For eac ...
Genetics
... MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. 1) The work of Gregor Mendel provided an answer to two prevailing hypotheses popular at the time. What were these two hypotheses? 1) _______ A) Self-fertilization is the rule in plants, while cross ...
... MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. 1) The work of Gregor Mendel provided an answer to two prevailing hypotheses popular at the time. What were these two hypotheses? 1) _______ A) Self-fertilization is the rule in plants, while cross ...
Incomplete Dominance
... dominance in humans occurs in the genes for curly hair. Inheriting a gene for curly hair from one parent and a gene for straight hair from the other parent will give a hair texture that is a blend of the two, wavy hair. ...
... dominance in humans occurs in the genes for curly hair. Inheriting a gene for curly hair from one parent and a gene for straight hair from the other parent will give a hair texture that is a blend of the two, wavy hair. ...
(XX) express twice as many genes as males (XY)?
... are gray body /large wings or back body/small wings. Conclusion: Genes are located on same chromosome and are inherited together (usually). Figure 15.9 ...
... are gray body /large wings or back body/small wings. Conclusion: Genes are located on same chromosome and are inherited together (usually). Figure 15.9 ...
What is a gene, post-ENCODE? History and updated definition
... This project represents a major milestone in the characterization of the human genome, and the current findings show a striking picture of complex molecular activity. While the landmark human genome sequencing surprised many with the small number (relative to simpler organisms) of protein-coding gen ...
... This project represents a major milestone in the characterization of the human genome, and the current findings show a striking picture of complex molecular activity. While the landmark human genome sequencing surprised many with the small number (relative to simpler organisms) of protein-coding gen ...
The Chromosome Theory of Inheritance
... Black Body and Normal Wings25% Black Body and Short Wings ...
... Black Body and Normal Wings25% Black Body and Short Wings ...
DNA and Gene Expression
... type-II (withdrawal, blunted affect) is more severe and more heritable • Some symptom dimensions (e.g., disorganization) more heritable than others ...
... type-II (withdrawal, blunted affect) is more severe and more heritable • Some symptom dimensions (e.g., disorganization) more heritable than others ...
ch. 12 Biotechnology-notes-ppt
... been produced from the originals by permission of the publisher. These illustrations may not be reproduced in any format for any purpose without express written permission from the publisher. • Unless otherwise noted, illustrations are credited to Pearson Education which have been borrowed from BIOL ...
... been produced from the originals by permission of the publisher. These illustrations may not be reproduced in any format for any purpose without express written permission from the publisher. • Unless otherwise noted, illustrations are credited to Pearson Education which have been borrowed from BIOL ...
01 Microevolution Unique Gene Pools and Genetic Variation NMSI
... • Evolution at this scale can be observed over short periods of time such as from one generation to the next. • Example: The frequency of a gene for pesticide resistance in a population of crop pests increases. • Such a change might come about because – natural selection favored the gene – the popul ...
... • Evolution at this scale can be observed over short periods of time such as from one generation to the next. • Example: The frequency of a gene for pesticide resistance in a population of crop pests increases. • Such a change might come about because – natural selection favored the gene – the popul ...
Genetics and Heredity Notes
... If the two traits are transmitted from parents to offspring as a package, the F1 spring would produce yellow, round seeds. The F2 offspring would produce two phenotypes (yellow + round; green + wrinkled) in a 3:1 ratio, just like a ...
... If the two traits are transmitted from parents to offspring as a package, the F1 spring would produce yellow, round seeds. The F2 offspring would produce two phenotypes (yellow + round; green + wrinkled) in a 3:1 ratio, just like a ...
new03
... Measuring the alleles uses electric field to separate the fragments according to their molecular weights (Using Southern blotting). ...
... Measuring the alleles uses electric field to separate the fragments according to their molecular weights (Using Southern blotting). ...
LINKAGE DATA a, the
... of Emerson a) required both methionine and histidine for normal growth. The histidine requirement resulted from a second mutation located a few units distal to the me-2 locus. It is proposed to designate the me-2 mutant isolation nlnnber P143m and the u P143h. Further information was sought for two ...
... of Emerson a) required both methionine and histidine for normal growth. The histidine requirement resulted from a second mutation located a few units distal to the me-2 locus. It is proposed to designate the me-2 mutant isolation nlnnber P143m and the u P143h. Further information was sought for two ...
Experiment 8 - WordPress.com
... The transformed cells contain the genes for GFP (green fluorescent protein) which allows the bacteria to display green fluorescence in the presence of arabinose and a UV light. GFP can be switched on and off. Arabinose, a biological molecule, allows GFP to turn on and, therefore, fluoresce. The a ...
... The transformed cells contain the genes for GFP (green fluorescent protein) which allows the bacteria to display green fluorescence in the presence of arabinose and a UV light. GFP can be switched on and off. Arabinose, a biological molecule, allows GFP to turn on and, therefore, fluoresce. The a ...
Mutations
... • Children born with this disorder cannot make an enzyme that is critical in breaking down fat and toxic substances in the brain. • The disease is terminal. Most will die before age ...
... • Children born with this disorder cannot make an enzyme that is critical in breaking down fat and toxic substances in the brain. • The disease is terminal. Most will die before age ...
Gene
A gene is a locus (or region) of DNA that encodes a functional RNA or protein product, and is the molecular unit of heredity. The transmission of genes to an organism's offspring is the basis of the inheritance of phenotypic traits. Most biological traits are under the influence of polygenes (many different genes) as well as the gene–environment interactions. Some genetic traits are instantly visible, such as eye colour or number of limbs, and some are not, such as blood type, risk for specific diseases, or the thousands of basic biochemical processes that comprise life.Genes can acquire mutations in their sequence, leading to different variants, known as alleles, in the population. These alleles encode slightly different versions of a protein, which cause different phenotype traits. Colloquial usage of the term ""having a gene"" (e.g., ""good genes,"" ""hair colour gene"") typically refers to having a different allele of the gene. Genes evolve due to natural selection or survival of the fittest of the alleles.The concept of a gene continues to be refined as new phenomena are discovered. For example, regulatory regions of a gene can be far removed from its coding regions, and coding regions can be split into several exons. Some viruses store their genome in RNA instead of DNA and some gene products are functional non-coding RNAs. Therefore, a broad, modern working definition of a gene is any discrete locus of heritable, genomic sequence which affect an organism's traits by being expressed as a functional product or by regulation of gene expression.