Use of a novel cassette to label phenotypically a cryptic plasmid of
... isolated on a Sau3A fragment which on insertion at a BamHI site regenerates a BamHI site at the downstream end-of-thetermin~i-^(GiTpAPG3,Fig. 1). However, downstream of the rrnBt, tz terminators from pKK223-3 there is no convenient restriction site before the bla promoter at position 4189 of the pBR ...
... isolated on a Sau3A fragment which on insertion at a BamHI site regenerates a BamHI site at the downstream end-of-thetermin~i-^(GiTpAPG3,Fig. 1). However, downstream of the rrnBt, tz terminators from pKK223-3 there is no convenient restriction site before the bla promoter at position 4189 of the pBR ...
The relationship between higher‑order chromatin structure and
... input chromatin fractions to a human chromosome 22q genomic array consisting of overlapping sequencing tiling path clones [21]. The average resolution of this array is 78 kb, but it contains regions where the clones are even smaller than the size of the chromatin fibres being examined (∼20 kb). The ...
... input chromatin fractions to a human chromosome 22q genomic array consisting of overlapping sequencing tiling path clones [21]. The average resolution of this array is 78 kb, but it contains regions where the clones are even smaller than the size of the chromatin fibres being examined (∼20 kb). The ...
Molecular cloning of a rhodopsin gene from salamander rods.
... contain a ~1.2 kb insert, including a full-length coding region of 1065 bp, a translation start codon ATG, a termination codon TAA, 87 bp of 5' noncoding sequence, and 68 bp of 3' noncoding sequence (Fig. 1). The sequence of the coding region was confirmed by sequencing five more independent clones. ...
... contain a ~1.2 kb insert, including a full-length coding region of 1065 bp, a translation start codon ATG, a termination codon TAA, 87 bp of 5' noncoding sequence, and 68 bp of 3' noncoding sequence (Fig. 1). The sequence of the coding region was confirmed by sequencing five more independent clones. ...
A genotype of Pp
... Pea plants have purple and white alleles for flower color. The allele for purple flowers is dominant and the allele for white flowers is recessive. If the allele for purple flowers is present, the plant will produce purple flowers. ...
... Pea plants have purple and white alleles for flower color. The allele for purple flowers is dominant and the allele for white flowers is recessive. If the allele for purple flowers is present, the plant will produce purple flowers. ...
SLOs - 3.3 Genetics small - Miss Jan`s Science Wikispace
... Explain what genes are Describe the relationship between genes are chromosomes Explain what a homologous pair is Explain what a centromere is Explain how a homologous pair of chromosomes can be identified Explain what a zygote is Explain what gametes are Explain what fertilisation is ...
... Explain what genes are Describe the relationship between genes are chromosomes Explain what a homologous pair is Explain what a centromere is Explain how a homologous pair of chromosomes can be identified Explain what a zygote is Explain what gametes are Explain what fertilisation is ...
Epigenetic Effects of Psychological Stressors in Humans
... Epigenetic mechanisms including DNA methylation and histone modifications are the link between environment and genome. These mechanisms control gene expression and determine the genetic outcome of an organism. The main well investigated epigenetic mechanisms are DNA methylation and histone modificat ...
... Epigenetic mechanisms including DNA methylation and histone modifications are the link between environment and genome. These mechanisms control gene expression and determine the genetic outcome of an organism. The main well investigated epigenetic mechanisms are DNA methylation and histone modificat ...
Abdominal Aortic Aneurysms(AAA)
... - The hallmark of MFS is abnormality of the medial layer of the aortic wall and the most threatening consequence of MFS is dilatation of the aortic root and the ascending aorta, which, if untreated, can result in fatal aortic ...
... - The hallmark of MFS is abnormality of the medial layer of the aortic wall and the most threatening consequence of MFS is dilatation of the aortic root and the ascending aorta, which, if untreated, can result in fatal aortic ...
A molecular method for assessing meiofauna diversity in marine
... Sediment samples were collected from East Beach at Fort DeSoto Park, St. Petersburg, FL, in 1.5mL microcentrifuge tubes and stored at -80°C. DNA was extracted from the sediment using an SDS-based extraction buffer and series of phenol, phenolchloroform, and chloroform extractions, and ethanol-precip ...
... Sediment samples were collected from East Beach at Fort DeSoto Park, St. Petersburg, FL, in 1.5mL microcentrifuge tubes and stored at -80°C. DNA was extracted from the sediment using an SDS-based extraction buffer and series of phenol, phenolchloroform, and chloroform extractions, and ethanol-precip ...
Recurrent Pregnancy Loss and Its Relation to Combined Parental
... enhanced blood coagulation and are involved in folic acid metabolism which influences DNA methylation. An association with RPL has also been reported for the Val34Leu polymorphism of the FXIII gene by leading to fibrin degradation. ...
... enhanced blood coagulation and are involved in folic acid metabolism which influences DNA methylation. An association with RPL has also been reported for the Val34Leu polymorphism of the FXIII gene by leading to fibrin degradation. ...
genomebiology.com
... are the source of ultra-conserved elements within mammalian genomes. However, with this exception there have been no systematic large-scale analyses of the impact of TEs on the transcriptomes of non-mammalian genomes. To address this issue we compiled a dataset of all TE families in the genomes of c ...
... are the source of ultra-conserved elements within mammalian genomes. However, with this exception there have been no systematic large-scale analyses of the impact of TEs on the transcriptomes of non-mammalian genomes. To address this issue we compiled a dataset of all TE families in the genomes of c ...
a-Aminoadipate aminotransferase from an extremely
... of AAA or lysine shortened the lag phase and improved the growth rate. The Kat2 homologue was therefore termed lysN. LysN recognizes not only 2-oxoadipate, an intermediate of lysine biosynthesis, but also 2-oxoisocaproate, 2-oxoisovalerate and 2-oxo-3-methylvalerate, intermediates of leucine, valine ...
... of AAA or lysine shortened the lag phase and improved the growth rate. The Kat2 homologue was therefore termed lysN. LysN recognizes not only 2-oxoadipate, an intermediate of lysine biosynthesis, but also 2-oxoisocaproate, 2-oxoisovalerate and 2-oxo-3-methylvalerate, intermediates of leucine, valine ...
RESISTANCE AS A RESPONSE TO STRESS
... activities of individual uncharacterized P450s from each other with a high degree of accuracy. The activity of a specific P450 towards an insecticide must be determined because its over-expression does not necessarily prove that it is the enzyme responsible for resistance. The over-expression of CYP ...
... activities of individual uncharacterized P450s from each other with a high degree of accuracy. The activity of a specific P450 towards an insecticide must be determined because its over-expression does not necessarily prove that it is the enzyme responsible for resistance. The over-expression of CYP ...
- Iranian Journal of Basic Medical Sciences
... 10- Kim SY, Park YJ, Kim WI, Lee SH, Chang CL, Kang SJ, et al. Molecular analysis of isoniazid resistance in Mycobacterium tuberculosis isolates recovered from South Korea. Diagn Microbiol Infect Dis 2003; 47:497-502. 11. Guo H, Seet Q, Denkin S, Parsons L, Zhang Y. Molecular characterization of iso ...
... 10- Kim SY, Park YJ, Kim WI, Lee SH, Chang CL, Kang SJ, et al. Molecular analysis of isoniazid resistance in Mycobacterium tuberculosis isolates recovered from South Korea. Diagn Microbiol Infect Dis 2003; 47:497-502. 11. Guo H, Seet Q, Denkin S, Parsons L, Zhang Y. Molecular characterization of iso ...
Disintegrin, hemorrhagic, and proteolytic activities of Mohave
... minimum essential medium supplemented with 10% fetal calf serum, sodium pyruvate, nonessential amino acids, lglutamine, and vitamins. T24 cells were incubated at 37 -C, in a humidified 5% CO2 – 95% air incubator. Cells were harvested, counted, and resuspended in medium containing 1% BSA at 5 105 c ...
... minimum essential medium supplemented with 10% fetal calf serum, sodium pyruvate, nonessential amino acids, lglutamine, and vitamins. T24 cells were incubated at 37 -C, in a humidified 5% CO2 – 95% air incubator. Cells were harvested, counted, and resuspended in medium containing 1% BSA at 5 105 c ...
Import, distribution and retail of gm
... The genetically modified carnation variety ‘Moonshadow’ was authorized in 1998 for production, import, distribution and retail in Europe. ‘Moonshadow’ was grown in Europe from December 1998 until July 1999. From 2001 ‘Moonshadow’ was imported from countries outside Europe, primarily from Ecuador. ‘M ...
... The genetically modified carnation variety ‘Moonshadow’ was authorized in 1998 for production, import, distribution and retail in Europe. ‘Moonshadow’ was grown in Europe from December 1998 until July 1999. From 2001 ‘Moonshadow’ was imported from countries outside Europe, primarily from Ecuador. ‘M ...
factor occupancy and gene expression Effects of sequence variation
... A complex interplay between transcription factors (TFs) and the genome regulates transcription. However, connecting variation in genome sequence with variation in TF binding and gene expression is challenging due to environmental differences between individuals and cell types. To address this proble ...
... A complex interplay between transcription factors (TFs) and the genome regulates transcription. However, connecting variation in genome sequence with variation in TF binding and gene expression is challenging due to environmental differences between individuals and cell types. To address this proble ...
factor occupancy and gene expression Effects of
... A complex interplay between transcription factors (TFs) and the genome regulates transcription. However, connecting variation in genome sequence with variation in TF binding and gene expression is challenging due to environmental differences between individuals and cell types. To address this proble ...
... A complex interplay between transcription factors (TFs) and the genome regulates transcription. However, connecting variation in genome sequence with variation in TF binding and gene expression is challenging due to environmental differences between individuals and cell types. To address this proble ...
The presence of two UvrB subunits in the UvrAB complex ensures
... damage recognition process the DNA is wrapped around the UvrB protein and this DNA wrap is dependent on ATP binding by UvrB (Verhoeven et al., 2001). DNA wrapping is expected to cause local melting of the DNA helix, thereby facilitating insertion of the b-hairpin of UvrB between the DNA strands. Fro ...
... damage recognition process the DNA is wrapped around the UvrB protein and this DNA wrap is dependent on ATP binding by UvrB (Verhoeven et al., 2001). DNA wrapping is expected to cause local melting of the DNA helix, thereby facilitating insertion of the b-hairpin of UvrB between the DNA strands. Fro ...
RIBOZYMES
... the self-cleavage reaction includes approximately 13 conserved or invariant "core" nucleotides, most of which are not involved in forming canonical Watson-Crick basepairs. The core region is flanked by Stems I, II and III, which are in general made of canonical Watson-Crick base-pairs but are othe ...
... the self-cleavage reaction includes approximately 13 conserved or invariant "core" nucleotides, most of which are not involved in forming canonical Watson-Crick basepairs. The core region is flanked by Stems I, II and III, which are in general made of canonical Watson-Crick base-pairs but are othe ...
factor occupancy and gene expression Effects of sequence variation
... A complex interplay between transcription factors (TFs) and the genome regulates transcription. However, connecting variation in genome sequence with variation in TF binding and gene expression is challenging due to environmental differences between individuals and cell types. To address this proble ...
... A complex interplay between transcription factors (TFs) and the genome regulates transcription. However, connecting variation in genome sequence with variation in TF binding and gene expression is challenging due to environmental differences between individuals and cell types. To address this proble ...
Gregor Mendel and Genetics
... round seeds like one of the two parents. When the F1 generation plants self-pollinated, however, their offspring—the F2 generation—showed all possible combinations of the two characteristics. Some had green round seeds, for example, and some had yellow wrinkled seeds. These combinations of character ...
... round seeds like one of the two parents. When the F1 generation plants self-pollinated, however, their offspring—the F2 generation—showed all possible combinations of the two characteristics. Some had green round seeds, for example, and some had yellow wrinkled seeds. These combinations of character ...
Aminoacylated tmRNA from Escherichia coli interacts with
... elongation factor Tu (EF-TU) and guanosine-59-triphosphate (GTP), or use another specific pathway+ Biochemical studies (Rudinger et al+, 1994) as well as the X-ray structures of two ternary complexes (Nissen et al+, 1995, 1999) indicate that tRNAs interact with prokaryotic EFTu-GTP via the aminoacyl ...
... elongation factor Tu (EF-TU) and guanosine-59-triphosphate (GTP), or use another specific pathway+ Biochemical studies (Rudinger et al+, 1994) as well as the X-ray structures of two ternary complexes (Nissen et al+, 1995, 1999) indicate that tRNAs interact with prokaryotic EFTu-GTP via the aminoacyl ...
Full-Text PDF
... reaction chambers that contained lyophilized RPA assays. A portable electronic reader and end-point fluorescence detection was used to identify the pathogen. Digital microfluidic (DMF) technology provides an alternative method for performing fully programmable automated microfluidic assays using nL ...
... reaction chambers that contained lyophilized RPA assays. A portable electronic reader and end-point fluorescence detection was used to identify the pathogen. Digital microfluidic (DMF) technology provides an alternative method for performing fully programmable automated microfluidic assays using nL ...
KAIE LOKK Comparative genome-wide DNA methylation
... methylation profiles have been shown to vary with age (Madrigano et al., 2012), and in response to environmental factors (Bind et al., 2012), nutritional factors, and pathogenic factors (Leonard et al., 2012; Liu et al., 2003). Disease-specific methylation patterns have also been observed (Montano e ...
... methylation profiles have been shown to vary with age (Madrigano et al., 2012), and in response to environmental factors (Bind et al., 2012), nutritional factors, and pathogenic factors (Leonard et al., 2012; Liu et al., 2003). Disease-specific methylation patterns have also been observed (Montano e ...
Metabolism of Macromolecules in Bacteria Treated
... Mixing the two virginiamycin components potentiated their antibiotic activity about 200-fold. In fact, a level of 0.5 pg./ml. of factors M and S induced an inhibitory effect comparable to that of IOO pg. of a single component. The numbers of colony-forming units after incubation for different period ...
... Mixing the two virginiamycin components potentiated their antibiotic activity about 200-fold. In fact, a level of 0.5 pg./ml. of factors M and S induced an inhibitory effect comparable to that of IOO pg. of a single component. The numbers of colony-forming units after incubation for different period ...
Gene
A gene is a locus (or region) of DNA that encodes a functional RNA or protein product, and is the molecular unit of heredity. The transmission of genes to an organism's offspring is the basis of the inheritance of phenotypic traits. Most biological traits are under the influence of polygenes (many different genes) as well as the gene–environment interactions. Some genetic traits are instantly visible, such as eye colour or number of limbs, and some are not, such as blood type, risk for specific diseases, or the thousands of basic biochemical processes that comprise life.Genes can acquire mutations in their sequence, leading to different variants, known as alleles, in the population. These alleles encode slightly different versions of a protein, which cause different phenotype traits. Colloquial usage of the term ""having a gene"" (e.g., ""good genes,"" ""hair colour gene"") typically refers to having a different allele of the gene. Genes evolve due to natural selection or survival of the fittest of the alleles.The concept of a gene continues to be refined as new phenomena are discovered. For example, regulatory regions of a gene can be far removed from its coding regions, and coding regions can be split into several exons. Some viruses store their genome in RNA instead of DNA and some gene products are functional non-coding RNAs. Therefore, a broad, modern working definition of a gene is any discrete locus of heritable, genomic sequence which affect an organism's traits by being expressed as a functional product or by regulation of gene expression.