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Secondary metabolism is a term for pathways and products
Secondary metabolism is a term for pathways and products

... Organic natural products are constructed of carbon, hydrogen, and oxygen atoms; frequently nitrogen atoms are also involved, and less frequently sulphur, phosphorus, chlorine, bromine, and iodine atoms. Organometalic compounds, especially metal complexes, also occur. The ultimate sources of these el ...
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File

... o 1.E.1 Physical and chemical processes can be depicted symbolically; when it is done, the illustration must conserve all atoms of all types. o 1.E.2 Conservation of atoms makes it possible to compute the masses of substances involved in physical and chemical processes. Chemical processes result in ...
Some basic concepts of chemistry
Some basic concepts of chemistry

... DEFINITE COMPOSITION (JOSEPH PROUST) Statement: A given chemical compound always contains the same elements combined together in definite proportion by mass, i.e., it has a fixed composition and does not depend on the method of its preparation or the source from which it has been obtained. This law ...
Personal Tutoring Help on Questions and Problems
Personal Tutoring Help on Questions and Problems

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Organic Molecules

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Section 2.9 Molar Mass: Counting Atoms by Weighing Them
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FREE Sample Here

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FREE Sample Here

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... When two elements C and O separately combine with H to from methane (CH) and water (H O) respectively it is very clear, that in methane 3g of C combine with 1g of hydrogen and in water (H O) 8g of O combine with the same (fixed) mass i.e. (1g) of H now when C and O combine with each other to form ca ...
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FREE Sample Here

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Isotopic labeling



Isotopic labeling (or isotopic labelling) is a technique used to track the passage of an isotope, or an atom with a variation, through a reaction, metabolic pathway, or cell. The reactant is 'labeled' by replacing specific atoms by their isotope. The reactant is then allowed to undergo the reaction. The position of the isotopes in the products is measured to determine the sequence the isotopic atom followed in the reaction or the cell's metabolic pathway. The nuclides used in isotopic labeling may be stable nuclides or radionuclides. In the latter case, the labeling is called radiolabeling.In isotopic labeling, there are multiple ways to detect the presence of labeling isotopes; through their mass, vibrational mode, or radioactive decay. Mass spectrometry detects the difference in an isotope's mass, while infrared spectroscopy detects the difference in the isotope's vibrational modes. Nuclear magnetic resonance detects atoms with different gyromagnetic ratios. The radioactive decay can be detected through an ionization chamber or autoradiographs of gels.An example of the use of isotopic labeling is the study of phenol (C6H5OH) in water by replacing common hydrogen (protium) with deuterium (deuterium labeling). Upon adding phenol to deuterated water (water containing D2O in addition to the usual H2O), the substitution of deuterium for the hydrogen is observed in phenol's hydroxyl group (resulting in C6H5OD), indicating that phenol readily undergoes hydrogen-exchange reactions with water. Only the hydroxyl group was affected, indicating that the other 5 hydrogen atoms did not participate in these exchange reactions.
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