Diapositiva 1 - ASCRS/ASOA 2008
... right and left eyes of a single subject. All the normal electrophoretic bands are clearly more intense (increased evaporation?) ...
... right and left eyes of a single subject. All the normal electrophoretic bands are clearly more intense (increased evaporation?) ...
Leukaemia Section t(4;17)(q12;q21) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... amino-terminal amino acids of FIP1L, including the FIP homology domain and 403 carboxyl-terminal amino acids of RARA, including the DNA and ligand binding domains, with replacement of FIP1L1 amino acid 429 (Valine) and RARA amino acid 60 (Threonine) into an Alanine. Oncogenesis All known chimeric RA ...
... amino-terminal amino acids of FIP1L, including the FIP homology domain and 403 carboxyl-terminal amino acids of RARA, including the DNA and ligand binding domains, with replacement of FIP1L1 amino acid 429 (Valine) and RARA amino acid 60 (Threonine) into an Alanine. Oncogenesis All known chimeric RA ...
Characterization of Proteins Structurally Related to Human N
... preparations used to raise the antisera were not in fact homogeneous, although they were judged to be so on the basis of a single protein band after electrophoresis in polyacrylamide gel and a single schlieren boundary in the ultracentrifuge (Srivastava et al., 1974a,b). A small amount of a highly i ...
... preparations used to raise the antisera were not in fact homogeneous, although they were judged to be so on the basis of a single protein band after electrophoresis in polyacrylamide gel and a single schlieren boundary in the ultracentrifuge (Srivastava et al., 1974a,b). A small amount of a highly i ...
Glycoblocks: a schematic three-dimensional
... dimensions has been successfully implemented already with the ribbon diagram (Richardson, 1985), which is the representation of choice for proteins, and has been implemented with minor local variations in all major structure visualization software. Perhaps unsurprisingly, carbohydrates have not been ...
... dimensions has been successfully implemented already with the ribbon diagram (Richardson, 1985), which is the representation of choice for proteins, and has been implemented with minor local variations in all major structure visualization software. Perhaps unsurprisingly, carbohydrates have not been ...
Leukaemia Section Chronic myelogenous leukaemia (CML) Atlas of Genetics and Cytogenetics
... is the occurrence of chromosome anomalies which are typical of a given BC phenotype (e.g. t(15;17) in a promyelocytic transformation, dic(9;12) in a CD10+ lymphoblastic BC...); +8, +19, +21, and i(17q) occur more often in myeloid -rather than lymphoid- blast ...
... is the occurrence of chromosome anomalies which are typical of a given BC phenotype (e.g. t(15;17) in a promyelocytic transformation, dic(9;12) in a CD10+ lymphoblastic BC...); +8, +19, +21, and i(17q) occur more often in myeloid -rather than lymphoid- blast ...
PDZ proteins retain and regulate membrane transporters in
... GOPC is also found in the plasma membrane when coexpressed in COS-7 cells with the plasma membrane protein frizzled (78) or with a dominant-negative dynamin 2 that blocks clathrin-mediated endocytosis (9). This suggests that GOPC undergoes dynamic trafficking between the Golgi apparatus and the plas ...
... GOPC is also found in the plasma membrane when coexpressed in COS-7 cells with the plasma membrane protein frizzled (78) or with a dominant-negative dynamin 2 that blocks clathrin-mediated endocytosis (9). This suggests that GOPC undergoes dynamic trafficking between the Golgi apparatus and the plas ...
Translation
... endomembrane system and proteins that are secreted from the cell • Ribosomes are identical and can switch from free to bound Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings ...
... endomembrane system and proteins that are secreted from the cell • Ribosomes are identical and can switch from free to bound Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings ...
A Ligand for CD5 Is CD5 - The Journal of Immunology
... Recognition by scavenger receptor cysteine-rich domains on membrane proteins regulates innate and adaptive immune responses. Two receptors expressed primarily on T cells, CD5 and CD6, are linked genetically and are structurally similar, both containing three scavenger receptor cysteine-rich domains ...
... Recognition by scavenger receptor cysteine-rich domains on membrane proteins regulates innate and adaptive immune responses. Two receptors expressed primarily on T cells, CD5 and CD6, are linked genetically and are structurally similar, both containing three scavenger receptor cysteine-rich domains ...
SMN, the Product of the Spinal Muscular Atrophy Gene, Binds
... We report here that SMN only interacts with its substrates after they are modified to dimethylarginines. This finding demonstrates that methylation of arginines, a very common modification which was discovered over 30 years ago and about whose function little is known, ...
... We report here that SMN only interacts with its substrates after they are modified to dimethylarginines. This finding demonstrates that methylation of arginines, a very common modification which was discovered over 30 years ago and about whose function little is known, ...
Cell-Free (In Vitro) Protein Expression
... Produce active/detectable proteins without radioactivity Indirect detection of biotin labeled proteins using streptavidin conjugates (HRP, AlkPhos) Use in many applications including pull downs, co-immunoprecipitations, mobility shift assays… ...
... Produce active/detectable proteins without radioactivity Indirect detection of biotin labeled proteins using streptavidin conjugates (HRP, AlkPhos) Use in many applications including pull downs, co-immunoprecipitations, mobility shift assays… ...
Role of protein methylation in chromatin remodeling and
... observed in several proteins containing SET domains (Rea et al., 2000; O'Carroll et al., 2000). The mammalian SET domain protein SUV39H1 methylates lysine 9 of histone H3, and the SET domain was important for this activity. The ability to methylate histones was observed in some but not all SET domai ...
... observed in several proteins containing SET domains (Rea et al., 2000; O'Carroll et al., 2000). The mammalian SET domain protein SUV39H1 methylates lysine 9 of histone H3, and the SET domain was important for this activity. The ability to methylate histones was observed in some but not all SET domai ...
Protein 4.2 interaction with hereditary spherocytosis mutants of the
... Residues Gly130 and Pro327 , the locations of the HS mutations G130R and P327R, are indicated. Residues 1−54 (red dotted line) were not resolved in the structure. The E40K HS mutation is located here and the approximate location of residue Glu40 is indicated on the structure. Residues 1−65 (red) are ...
... Residues Gly130 and Pro327 , the locations of the HS mutations G130R and P327R, are indicated. Residues 1−54 (red dotted line) were not resolved in the structure. The E40K HS mutation is located here and the approximate location of residue Glu40 is indicated on the structure. Residues 1−65 (red) are ...
Identification and characterization of novel interaction partners of
... Mirjana Gagic, Thomas Keßler and H.- Arno J. Müller; Institut für Genetik, Heinrich-Heine Universität Düsseldorf, Germany ...
... Mirjana Gagic, Thomas Keßler and H.- Arno J. Müller; Institut für Genetik, Heinrich-Heine Universität Düsseldorf, Germany ...
Gene Section SAV1 (Salvador homolog 1 (Drosophila)) Atlas of Genetics and Cytogenetics
... The longest SAV1 mRNA transcript of 3.0 kb encodes an open reading frame (ORF) of 1152 bases and untranslated regions of 338 and 1541 bases at the 5’ and 3’ ends, respectively. No splice variants have been reported for SAV1. Smaller transcripts of 1.8 kb and 2.1 kb, encoding the identical ORF, have ...
... The longest SAV1 mRNA transcript of 3.0 kb encodes an open reading frame (ORF) of 1152 bases and untranslated regions of 338 and 1541 bases at the 5’ and 3’ ends, respectively. No splice variants have been reported for SAV1. Smaller transcripts of 1.8 kb and 2.1 kb, encoding the identical ORF, have ...
Diseases of the Immune System lec.4
... tissue damage and functional compromise. These abnormal fibrils are produced by the aggregation of misfolded proteins or protein fragments. Pathogenesis of Amyloid Deposition All amyloid deposits are composed of nonbranching fibrils, each formed of β-sheet polypeptide chains that are wound together. ...
... tissue damage and functional compromise. These abnormal fibrils are produced by the aggregation of misfolded proteins or protein fragments. Pathogenesis of Amyloid Deposition All amyloid deposits are composed of nonbranching fibrils, each formed of β-sheet polypeptide chains that are wound together. ...
Molecular mechanisms of complement evasion: learning from
... CCP6–CCP8 and by the C-terminal domains CCP19–CCP20 (these C-terminal domains form a ternary complex with both surface glycans and the TED domain of C3b)46,47. Domains CCP1–CCP4 harbour the functional activity of factor H48. The structure of C3b in complex with CCP1–CCP4 of factor H shows that the C ...
... CCP6–CCP8 and by the C-terminal domains CCP19–CCP20 (these C-terminal domains form a ternary complex with both surface glycans and the TED domain of C3b)46,47. Domains CCP1–CCP4 harbour the functional activity of factor H48. The structure of C3b in complex with CCP1–CCP4 of factor H shows that the C ...
Determination of Binding Sites of Cadherin Peptides on the EC1
... the binding activity of the N-terminus of E-cadherin. At low protein concentration, intramolecular binding of tryptophan-2 (W2) side-chain into the W2 pocket on the same molecule was detected via chemical shift changes. However, at high protein concentration, the X-ray structure indicates that two m ...
... the binding activity of the N-terminus of E-cadherin. At low protein concentration, intramolecular binding of tryptophan-2 (W2) side-chain into the W2 pocket on the same molecule was detected via chemical shift changes. However, at high protein concentration, the X-ray structure indicates that two m ...
WAVE/Scars in platelets
... Ectopic expression of WAVEs and interacting proteins in Cos7 cells The full-length cDNA3,29 of human WAVE/Scars (WAVE1 [Genbank Accession no. D87459 as KIAA0269], WAVE2 [AB026542], and WAVE3 [AB026543]) was subcloned into FLAG-tagged pBluescript and then constructed in the pEF-BOS vector.29,30 Human ...
... Ectopic expression of WAVEs and interacting proteins in Cos7 cells The full-length cDNA3,29 of human WAVE/Scars (WAVE1 [Genbank Accession no. D87459 as KIAA0269], WAVE2 [AB026542], and WAVE3 [AB026543]) was subcloned into FLAG-tagged pBluescript and then constructed in the pEF-BOS vector.29,30 Human ...
G PROTEIN βγ SUBUNITS
... subunits vary between 35 and 39 kDa. If all Gβ and Gγ pairs could form, the number of potential Gβγ subunit pairs would exceed the known number of Gα s by a factor of three. While it appears that most pairs can indeed form, there are exceptions (16–19). For example, the Gβ1 subunit can combine with ...
... subunits vary between 35 and 39 kDa. If all Gβ and Gγ pairs could form, the number of potential Gβγ subunit pairs would exceed the known number of Gα s by a factor of three. While it appears that most pairs can indeed form, there are exceptions (16–19). For example, the Gβ1 subunit can combine with ...
Print
... atoms known as the tertiary structure. The folding takes place in such a way that the hydrophobic residues get buried to form the core while the hydrophilic amino acids remain on the surface in contact with the polar surroundings. 4. Quaternary structure: Many proteins have more than one polypeptide ...
... atoms known as the tertiary structure. The folding takes place in such a way that the hydrophobic residues get buried to form the core while the hydrophilic amino acids remain on the surface in contact with the polar surroundings. 4. Quaternary structure: Many proteins have more than one polypeptide ...
Protein domain
A protein domain is a conserved part of a given protein sequence and (tertiary) structure that can evolve, function, and exist independently of the rest of the protein chain. Each domain forms a compact three-dimensional structure and often can be independently stable and folded. Many proteins consist of several structural domains. One domain may appear in a variety of different proteins. Molecular evolution uses domains as building blocks and these may be recombined in different arrangements to create proteins with different functions. Domains vary in length from between about 25 amino acids up to 500 amino acids in length. The shortest domains such as zinc fingers are stabilized by metal ions or disulfide bridges. Domains often form functional units, such as the calcium-binding EF hand domain of calmodulin. Because they are independently stable, domains can be ""swapped"" by genetic engineering between one protein and another to make chimeric proteins.