shRNA FAQ - Functional Genomics Facility
... hairpin sequences are each cloned into the pLKO.1 vector and sequence-verified. A minimum of 3-5 shRNA constructs are created for each target gene to provide varying levels of knockdown and to target different regions of mRNA transcript. Sigma-Aldrich guarantees that upon purchase of the entire clon ...
... hairpin sequences are each cloned into the pLKO.1 vector and sequence-verified. A minimum of 3-5 shRNA constructs are created for each target gene to provide varying levels of knockdown and to target different regions of mRNA transcript. Sigma-Aldrich guarantees that upon purchase of the entire clon ...
crosses. - Aurora City Schools
... The principle of independent assortment states that genes for different traits can segregate independently during the formation of gametes. Mendel wondered if the segregation of one pair of alleles affects another pair. Mendel performed an experiment that followed two different genes as they passed ...
... The principle of independent assortment states that genes for different traits can segregate independently during the formation of gametes. Mendel wondered if the segregation of one pair of alleles affects another pair. Mendel performed an experiment that followed two different genes as they passed ...
The Role of the ameioticl Gene in the Initiation of Meiosis
... elements (LE) were aligned along the entirelength of the bivalents and thekinetochores were clearly visible. Sometimes the typical tripartite structure of SCs (two LEs and one central element, CE) was observed (Figure 3b). We did not find homologous pairing abnormalities except in a few casesof inte ...
... elements (LE) were aligned along the entirelength of the bivalents and thekinetochores were clearly visible. Sometimes the typical tripartite structure of SCs (two LEs and one central element, CE) was observed (Figure 3b). We did not find homologous pairing abnormalities except in a few casesof inte ...
Do gametes woo? Evidence for non-random unions at
... 1 WT). Results for one sex- and both sex-TRD are remarkably similar with an excess of heterozygotes arising when TR <0.50, and a deficiency when TR>0.50 (Fig. 2). Thus, excess heterozygosity is found when the wildtype allele is favored and a deficiency when the m allele is favored. For Dnd1 and Ago2 ...
... 1 WT). Results for one sex- and both sex-TRD are remarkably similar with an excess of heterozygotes arising when TR <0.50, and a deficiency when TR>0.50 (Fig. 2). Thus, excess heterozygosity is found when the wildtype allele is favored and a deficiency when the m allele is favored. For Dnd1 and Ago2 ...
File - interACT - American College of Toxicology
... Zhejiang University in Hangzhou, China, and received her Ph.D. Degree with honors in University of Kansas Medical Center. Julia currently is an Assistant Professor in Toxicology in the Department of Environmental and Occupational Health Sciences. She is a recipient of the Sheldon D. Murphy Endowed C ...
... Zhejiang University in Hangzhou, China, and received her Ph.D. Degree with honors in University of Kansas Medical Center. Julia currently is an Assistant Professor in Toxicology in the Department of Environmental and Occupational Health Sciences. She is a recipient of the Sheldon D. Murphy Endowed C ...
Journal of Bacteriology
... ously been located in the phoB gene, which codes for a positive regulatory protein of thepho regulon (23). Class 2 mutants have an altered LPS, as indicated by their changed sensitivity towards the LPS-specific phages T3, T7, and P1 or C21 (11). One subclass of these mutants, of which strain CE1182 ...
... ously been located in the phoB gene, which codes for a positive regulatory protein of thepho regulon (23). Class 2 mutants have an altered LPS, as indicated by their changed sensitivity towards the LPS-specific phages T3, T7, and P1 or C21 (11). One subclass of these mutants, of which strain CE1182 ...
x-linked mental retardation
... agreement with others (see the XLMR Genes Update web site in the Online links box), we found it useful to maintain this separation in this review. About 140 syndromic forms of XLMR have been described so far. In 66 of these, including several that have since been found to be allelic, the causative g ...
... agreement with others (see the XLMR Genes Update web site in the Online links box), we found it useful to maintain this separation in this review. About 140 syndromic forms of XLMR have been described so far. In 66 of these, including several that have since been found to be allelic, the causative g ...
Genetics - Garnet Valley School District
... plant that is heterozygous for green color and homozygous for short shape is crossed with a plant that is homozygous for striped color and heterosygous for short shape. What proportion of their offspring will be striped and short? ...
... plant that is heterozygous for green color and homozygous for short shape is crossed with a plant that is homozygous for striped color and heterosygous for short shape. What proportion of their offspring will be striped and short? ...
A Maize Glutaredoxin Gene, Abphyl2, Regulates
... (abph1) mutants suggest the importance of auxin and cytokinin signaling for control of phyllotaxy. However, whether additional regulators control these patterns is poorly understood. Here, we report a new dominant maize mutant, Aberrant phyllotaxy2 (Abph2), in which the shoot meristems are enlarged ...
... (abph1) mutants suggest the importance of auxin and cytokinin signaling for control of phyllotaxy. However, whether additional regulators control these patterns is poorly understood. Here, we report a new dominant maize mutant, Aberrant phyllotaxy2 (Abph2), in which the shoot meristems are enlarged ...
worksheet
... between 90 and 120 days before being broken down and recycled by the body. There are more than 400 different types of anemia. These can be divided into three basic groups. Anemias caused by: ...
... between 90 and 120 days before being broken down and recycled by the body. There are more than 400 different types of anemia. These can be divided into three basic groups. Anemias caused by: ...
Peer-reviewed Article PDF
... mainly in the liver, vectors derived from a recombinant retrovirus can efficiently transduce the PAH cDNA into PAH-deficient hepatocytes in vitro, but the transduction efficiency is low in vivo [18]. Beyond this barrier, studies have been initiated in which vectors derived from a recombinant adenovi ...
... mainly in the liver, vectors derived from a recombinant retrovirus can efficiently transduce the PAH cDNA into PAH-deficient hepatocytes in vitro, but the transduction efficiency is low in vivo [18]. Beyond this barrier, studies have been initiated in which vectors derived from a recombinant adenovi ...
Risks from GMOs due to Horizontal Gene Transfer
... recipient cell. (2) MGEs can alter the function of genes in the vicinity of the insertion in the host genome. These alterations can include disruption or inactivation of genes at the site of insertion. Conversely, insertional mutagenesis by an MGE can also result in benefits to the host such as prov ...
... recipient cell. (2) MGEs can alter the function of genes in the vicinity of the insertion in the host genome. These alterations can include disruption or inactivation of genes at the site of insertion. Conversely, insertional mutagenesis by an MGE can also result in benefits to the host such as prov ...
Chromatin DNA Methylayion
... Model 1: DNA methylation patterns are fixed during development maintained faithfully by the maintenance methyltransferase in somatic cells CH3 ...
... Model 1: DNA methylation patterns are fixed during development maintained faithfully by the maintenance methyltransferase in somatic cells CH3 ...
Brooker Chapter 22
... During transcription of the proviral DNA, the proto-oncogene may be included in the RNA transcript This RNA transcript can then recombine with an RNA retroviral genome within the cell This results in a retrovirus that contains an oncogene ...
... During transcription of the proviral DNA, the proto-oncogene may be included in the RNA transcript This RNA transcript can then recombine with an RNA retroviral genome within the cell This results in a retrovirus that contains an oncogene ...
Simplified Insertion of Transgenes Onto Balancer Chromosomes via
... inserted onto balancers using a random and inefficient process. Here we describe balancer chromosomes that can be directly targeted with transgenes of interest via recombinase-mediated cassette exchange (RMCE). ...
... inserted onto balancers using a random and inefficient process. Here we describe balancer chromosomes that can be directly targeted with transgenes of interest via recombinase-mediated cassette exchange (RMCE). ...
Parental Methamphetamine Exposure Affects Offspring`s Behavior and
... • Methamphetamine addiction is associated with psychotic behavior and long term cognitive impairment. • Surveys of past-month illicit drug use among females aged 15-44 showed that 6-7% of these women were pregnant, and continued to use drugs during all three trimesters of pregnancy; 2,000-3,000 abus ...
... • Methamphetamine addiction is associated with psychotic behavior and long term cognitive impairment. • Surveys of past-month illicit drug use among females aged 15-44 showed that 6-7% of these women were pregnant, and continued to use drugs during all three trimesters of pregnancy; 2,000-3,000 abus ...
Genetic determinants of sense organ identity in
... sense organs: the mono-innervated external sense organs (mes), the poly-innervated external sense organs (p-es) and the chordotonal internal sense organs (ch). The three types of organs differ in their lineage, morphology and neuronal connectivity, thereby providing a useful system to examine the ge ...
... sense organs: the mono-innervated external sense organs (mes), the poly-innervated external sense organs (p-es) and the chordotonal internal sense organs (ch). The three types of organs differ in their lineage, morphology and neuronal connectivity, thereby providing a useful system to examine the ge ...
VegT activates Bix4 to specify endodermal
... maternal VegT RNA and is activated in animal caps by Xbra and VegT Expression of Bix4 was analysed in embryos in which maternal VegT transcripts were depleted by injection of antisense oligonucleotides into the oocyte. Previous work indicates that endodermin, a pan-endodermal marker (Sasai et al., 1 ...
... maternal VegT RNA and is activated in animal caps by Xbra and VegT Expression of Bix4 was analysed in embryos in which maternal VegT transcripts were depleted by injection of antisense oligonucleotides into the oocyte. Previous work indicates that endodermin, a pan-endodermal marker (Sasai et al., 1 ...
Module 1: Introduction
... with your list (hopefully part of experiment design! ) – Summarize biological processes or other aspects of gene function – Controller for a process (TF) ...
... with your list (hopefully part of experiment design! ) – Summarize biological processes or other aspects of gene function – Controller for a process (TF) ...
MicroReview Paradigms of plasmid organization
... can occur by homologous exchanges, by transposition events and by processes that we still do not understand. Some survival functions depend on just one genetic locus, but most depend on at least two ± even replication systems dependent on a single plasmid-encoded protein also need a cis-acting repli ...
... can occur by homologous exchanges, by transposition events and by processes that we still do not understand. Some survival functions depend on just one genetic locus, but most depend on at least two ± even replication systems dependent on a single plasmid-encoded protein also need a cis-acting repli ...
Mathews and Kramer FINAL
... meristem identity is the differential expression of complementary identity programs. These genetic modules are complex but, in Arabidopsis, inflorescence identity is defined by expression of loci such as TERMINAL FLOWER1 (TFL1), AGL24 and SUPPRESSOR OF CONSTANS (SOC1) (Lee & Lee, 2010), while floral ...
... meristem identity is the differential expression of complementary identity programs. These genetic modules are complex but, in Arabidopsis, inflorescence identity is defined by expression of loci such as TERMINAL FLOWER1 (TFL1), AGL24 and SUPPRESSOR OF CONSTANS (SOC1) (Lee & Lee, 2010), while floral ...
8th grade Chapter 8
... Together these are called our genome. B. Within a species (group of related organisms) each organism has the same number of chromosomes. C. However, genome size (the number of chromosomes) changes from one species to the next. D. For example a fruit fly has a genome of 8 chromosomes, whereas a dog h ...
... Together these are called our genome. B. Within a species (group of related organisms) each organism has the same number of chromosomes. C. However, genome size (the number of chromosomes) changes from one species to the next. D. For example a fruit fly has a genome of 8 chromosomes, whereas a dog h ...
Using GO to improve text information access
... or phrase will have more than one sense if it means something different in different contexts. The most obvious example of a term having more than one sense is when it can be used to refer to more than one distinct biomedical entity. For example, {ATP6V1H; ATPase, H+ transporting, lysosomal 50/57kDa ...
... or phrase will have more than one sense if it means something different in different contexts. The most obvious example of a term having more than one sense is when it can be used to refer to more than one distinct biomedical entity. For example, {ATP6V1H; ATPase, H+ transporting, lysosomal 50/57kDa ...