Gene targeting by hybridization-hydrolysis process
... All cDNA libraries obtained after each experiment had a significant number of recombinant clones (>106 cfu). Their corresponding DNA was precisely quantified for further PCR application. Figure 5 shows a multiplex PCR analysis carried out with the two libraries obtained before or after targeting thr ...
... All cDNA libraries obtained after each experiment had a significant number of recombinant clones (>106 cfu). Their corresponding DNA was precisely quantified for further PCR application. Figure 5 shows a multiplex PCR analysis carried out with the two libraries obtained before or after targeting thr ...
The regulation of expression of the porin gene ompC
... did not achieve a rate that was in balance with the growth rate. Further, induction of the ompCgene at acid external pH was dependent upon the presence of glucose as sole carbon source; growth with either glycerol or succinate as sole carbon source reduced induction of ompC at acid pH. Osmotic induc ...
... did not achieve a rate that was in balance with the growth rate. Further, induction of the ompCgene at acid external pH was dependent upon the presence of glucose as sole carbon source; growth with either glycerol or succinate as sole carbon source reduced induction of ompC at acid pH. Osmotic induc ...
GIN Transposons: Genetic Elements Linking Retrotransposons and
... indeed substantially more similar to GIN1 than the sequences previously described as its closest relatives, derived from retrotransposons of the Mdg1 clade (Lloréns and Marı́n 2001). Therefore, all the animal sequences that were potentially interesting were selected and phylogenetic trees were buil ...
... indeed substantially more similar to GIN1 than the sequences previously described as its closest relatives, derived from retrotransposons of the Mdg1 clade (Lloréns and Marı́n 2001). Therefore, all the animal sequences that were potentially interesting were selected and phylogenetic trees were buil ...
Solid Tumour Section tumors Atlas of Genetics and Cytogenetics
... one, have deletions encompassing the multiple endocrine neoplasia type I, MEN1, locus and a second region about 3 Mb distal to MEN1. Angiomyo-lipoma: few cases have been investigated cytogenetically; the only recurrent change identified has been trisomy 7; however, interphase FISH analysis did not r ...
... one, have deletions encompassing the multiple endocrine neoplasia type I, MEN1, locus and a second region about 3 Mb distal to MEN1. Angiomyo-lipoma: few cases have been investigated cytogenetically; the only recurrent change identified has been trisomy 7; however, interphase FISH analysis did not r ...
Chp 13 Meiosis and Sexual Life Cycles
... ï Human somatic cells contain 46 chromosomes distinguishable by differences in size, position of the centromere, and staining or banding pattern. ï Using these criteria, chromosomes from a photomicrograph can be matched into homologous pairs and arranged in a standard sequence to produce a karyotyp ...
... ï Human somatic cells contain 46 chromosomes distinguishable by differences in size, position of the centromere, and staining or banding pattern. ï Using these criteria, chromosomes from a photomicrograph can be matched into homologous pairs and arranged in a standard sequence to produce a karyotyp ...
S1 Table. Experimental evidence supporting interactions between
... Hardtke, C.S., and T. Berleth. 1998. The Arabidopsis gene MONOPTEROS encodes a transcription factor mediating embryo axis formation and vascular development. 1405-1411 pp. Hass, C., J. Lohrmann, V. Albrecht, U. Sweere, F. Hummel, S.D. Yoo, I. Hwang, T. Zhu, E. Schafer, J. Kudla, and K. Harter. 2004. ...
... Hardtke, C.S., and T. Berleth. 1998. The Arabidopsis gene MONOPTEROS encodes a transcription factor mediating embryo axis formation and vascular development. 1405-1411 pp. Hass, C., J. Lohrmann, V. Albrecht, U. Sweere, F. Hummel, S.D. Yoo, I. Hwang, T. Zhu, E. Schafer, J. Kudla, and K. Harter. 2004. ...
The Optimal Discovery Procedure II: Applications to Comparative
... A microarray measures the abundance of mRNA transcripts of thousands of genes from a single biological sample (1, 2). Of much recent interest has been the problem of identifying genes that are differentially expressed across varying biological conditions based on microarray data (3). It is now possi ...
... A microarray measures the abundance of mRNA transcripts of thousands of genes from a single biological sample (1, 2). Of much recent interest has been the problem of identifying genes that are differentially expressed across varying biological conditions based on microarray data (3). It is now possi ...
Cells: A Multiple Time Point Analysis Chronic Lymphocytic
... includes preferential segregation of somatic point mutations yielding an amino acid replacement (R mutations) within the complementarity-determining regions (CDRs), and various degrees of intraclonal diversification, as assessed by variation in the frequency and distribution of somatic point mutatio ...
... includes preferential segregation of somatic point mutations yielding an amino acid replacement (R mutations) within the complementarity-determining regions (CDRs), and various degrees of intraclonal diversification, as assessed by variation in the frequency and distribution of somatic point mutatio ...
U2Word
... subsequent codons are altered. b. Consider a sentence (gene) consisting of the words (codons): THE BIG RED DOG ATE ALL THE PIE. Insertion of one letter (base pair) yields: THE BIM GRE DDO GAT EAL LTH EPI E. Deletion of a single letter (bp) from the original sentence yields: THE BIG RDD OGA TEA LLT H ...
... subsequent codons are altered. b. Consider a sentence (gene) consisting of the words (codons): THE BIG RED DOG ATE ALL THE PIE. Insertion of one letter (base pair) yields: THE BIM GRE DDO GAT EAL LTH EPI E. Deletion of a single letter (bp) from the original sentence yields: THE BIG RDD OGA TEA LLT H ...
Polymorphic miRNA-mediated gene regulation: contribution to
... miRNA precursor hairpins do not have specific consensus sequences. It is their secondary structure that positions the double-stranded RNA-binding protein DGCR8 (known as Pasha in Drosophila) such that its ribonuclease III partner Drosha cleaves the stem at approximately one helical turn from the bas ...
... miRNA precursor hairpins do not have specific consensus sequences. It is their secondary structure that positions the double-stranded RNA-binding protein DGCR8 (known as Pasha in Drosophila) such that its ribonuclease III partner Drosha cleaves the stem at approximately one helical turn from the bas ...
Peter Kunzmann Metaphors in the Language of Darwinism
... overlap o f „useful tool” which is the shared feature o f „machine” in the ordinary sense and bodies serving their genes. (Stove’s funny remark about „sex mad prime numbers” only shows how indistinct these underlying comparisons sometimes are.) What makes the difference between „sex mad prime number ...
... overlap o f „useful tool” which is the shared feature o f „machine” in the ordinary sense and bodies serving their genes. (Stove’s funny remark about „sex mad prime numbers” only shows how indistinct these underlying comparisons sometimes are.) What makes the difference between „sex mad prime number ...
Lesson 17: Patterns of Inheritance (3
... carry traits that can be passed from one generation to the next. Alleles are different forms of a gene. Two alleles make up one gene. For every trait, each parent passes on one allele to the offspring. Each offspring has at least two alleles for every trait. The expression of physical characteristic ...
... carry traits that can be passed from one generation to the next. Alleles are different forms of a gene. Two alleles make up one gene. For every trait, each parent passes on one allele to the offspring. Each offspring has at least two alleles for every trait. The expression of physical characteristic ...
Sulfuricella denitrificans gen. nov., sp. nov., a sulfur
... phylogeny of this gene is only partially consistent with that based on the 16S rRNA gene, and the novel strain belonged to the cluster comprising betaproteobacteria (Supplementary Fig. S3). Phylogenetic analyses of multiple genes indicated the novelty of strain skB26T. Among genera of sulfur-oxidizi ...
... phylogeny of this gene is only partially consistent with that based on the 16S rRNA gene, and the novel strain belonged to the cluster comprising betaproteobacteria (Supplementary Fig. S3). Phylogenetic analyses of multiple genes indicated the novelty of strain skB26T. Among genera of sulfur-oxidizi ...
Familial Mediterranean Fever (FMF) factsheet for patients
... Alternatively, there may be little effect on the final product (if you used canola oil instead of corn oil). Similarly, a mutation in a gene may have a number of different effects. Some mutations have minimal effects or no effects either on the proteins produced or on the person’s health. Other muta ...
... Alternatively, there may be little effect on the final product (if you used canola oil instead of corn oil). Similarly, a mutation in a gene may have a number of different effects. Some mutations have minimal effects or no effects either on the proteins produced or on the person’s health. Other muta ...
The cyanobacterial genome core and the origin of photosynthesis
... and Chloroflexi (RC2-type) use bound quinones as electron acceptors and are similar to the cyanobacterial PSII. Since all these phyla, except for Cyanobacteria and, perhaps, Chlorobi, also contain non-photosynthetic representatives, evolution of photosynthesis must have included frequent loss of pho ...
... and Chloroflexi (RC2-type) use bound quinones as electron acceptors and are similar to the cyanobacterial PSII. Since all these phyla, except for Cyanobacteria and, perhaps, Chlorobi, also contain non-photosynthetic representatives, evolution of photosynthesis must have included frequent loss of pho ...
Chapter 14: The Human Genome Section 14
... British geneticist Mary Lyon discovered that in female cells, one X chromosome is randomly switched off. This chromosome forms a dense region in the nucleus known as a Barr body. Barr bodies are generally not found in males because their single X chromosome is still active. Slide 54 of 25 End Show ...
... British geneticist Mary Lyon discovered that in female cells, one X chromosome is randomly switched off. This chromosome forms a dense region in the nucleus known as a Barr body. Barr bodies are generally not found in males because their single X chromosome is still active. Slide 54 of 25 End Show ...
Signed Reversal Distance
... that the number of cycles in the breakpoint graph changes by at most 1 with any reversal of C1 , which immediately yields the following lemma. Lemma 1. d(C1 , C2 ) ≥ n + 1 − c, where c is the number of cycles in B(C1 , C2 ). For our particular instance of C1 , B(C1 , C2 ) will have 6 cycles, implyin ...
... that the number of cycles in the breakpoint graph changes by at most 1 with any reversal of C1 , which immediately yields the following lemma. Lemma 1. d(C1 , C2 ) ≥ n + 1 − c, where c is the number of cycles in B(C1 , C2 ). For our particular instance of C1 , B(C1 , C2 ) will have 6 cycles, implyin ...
chromosome disorders.
... • Most aneuploid patients have either trisomy or, less often, monosomy • Trisomy can exist for any part of the genome, but trisomy for a whole chromosome is rarely compatible with life. • Monosomy for an entire chromosome is almost always lethal; an important exception is monosomy for the X chromoso ...
... • Most aneuploid patients have either trisomy or, less often, monosomy • Trisomy can exist for any part of the genome, but trisomy for a whole chromosome is rarely compatible with life. • Monosomy for an entire chromosome is almost always lethal; an important exception is monosomy for the X chromoso ...
Transcripts of the MHM region on the chicken Z chromosome
... The male hypermethylated (MHM) region, located near the middle of the short arm of the Z chromosome of chickens, consists of approximately 210 tandem repeats of a BamHI 2.2-kb sequence unit. Cytosines of the CpG dinucleotides of this region are extensively methylated on the two Z chromosomes in the ...
... The male hypermethylated (MHM) region, located near the middle of the short arm of the Z chromosome of chickens, consists of approximately 210 tandem repeats of a BamHI 2.2-kb sequence unit. Cytosines of the CpG dinucleotides of this region are extensively methylated on the two Z chromosomes in the ...
