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... Diversity of the functional genes encoding dissimilatory nitrite reductase was investigated for the first time in denitrifying halobenzoate degrading bacteria and in two 4-chlorobenzoate degrading denitrifying consortia. Nitrite reductase genes were PCR-amplified with degenerate primers (specific to ...
... Diversity of the functional genes encoding dissimilatory nitrite reductase was investigated for the first time in denitrifying halobenzoate degrading bacteria and in two 4-chlorobenzoate degrading denitrifying consortia. Nitrite reductase genes were PCR-amplified with degenerate primers (specific to ...
Long time no see: the Type and Contre-type concept
... continue with the example of DS. The analysis of partial trisomies of chromosome 21 led to the concept of the DS critical region, according to which single phenotypic traits of DS could each be related to a single triplicated gene. Although the expression is still being used, the concept is hard to ...
... continue with the example of DS. The analysis of partial trisomies of chromosome 21 led to the concept of the DS critical region, according to which single phenotypic traits of DS could each be related to a single triplicated gene. Although the expression is still being used, the concept is hard to ...
Simplified global gene expression profiling
... performed using the AmpliSeqRNA plug-in with gene-level transcript quantification from sequence-read data. This plug-in is included in Torrent Suite Software, which comes with each Ion Torrent™ sequencer. The AmpliSeqRNA plug-in can be automatically executed upon completion of a sequencing run and i ...
... performed using the AmpliSeqRNA plug-in with gene-level transcript quantification from sequence-read data. This plug-in is included in Torrent Suite Software, which comes with each Ion Torrent™ sequencer. The AmpliSeqRNA plug-in can be automatically executed upon completion of a sequencing run and i ...
A GO annotation is
... annotations for analysis • Unannotated is not unknown - where there is no evidence in the literature for a process, function or location the gene product is annotated to the appropriate ontology’s root node with an ‘ND’ evidence code (no biological data), thereby distinguishing between unannotated a ...
... annotations for analysis • Unannotated is not unknown - where there is no evidence in the literature for a process, function or location the gene product is annotated to the appropriate ontology’s root node with an ‘ND’ evidence code (no biological data), thereby distinguishing between unannotated a ...
Use of Gene-Specific Primer Cocktails for First
... fold and still generate PCR product from common and therefore may not correspond to the D3 gene b transcripts; rare transcripts can be detected after a 100(Figure 1c). Strikingly, the amount of PCR product for fold dilution. It has also been shown that two-step each set of primers differed significa ...
... fold and still generate PCR product from common and therefore may not correspond to the D3 gene b transcripts; rare transcripts can be detected after a 100(Figure 1c). Strikingly, the amount of PCR product for fold dilution. It has also been shown that two-step each set of primers differed significa ...
Effective transfer of chromosomes carrying leaf rust resistance
... chromosomes was chosen from BC2F2 hybrids for the molecular analysis. The products of Lr22a primers amplification resulted in 135 base pairs (bp) and 167 bp bands, which are characteristic for susceptible genotypes, according Hiebert et al. (2007). The PCR reaction using Lr32 primers resulted in 261 ...
... chromosomes was chosen from BC2F2 hybrids for the molecular analysis. The products of Lr22a primers amplification resulted in 135 base pairs (bp) and 167 bp bands, which are characteristic for susceptible genotypes, according Hiebert et al. (2007). The PCR reaction using Lr32 primers resulted in 261 ...
You`re one in a googol: optimizing genes for protein expression
... tRNA levels, and by changing the context and copy number of the gene itself (Hannig & Makrides 1998; Baneyx 1999; Aricescu et al. 2006). Despite so many options, natural genes are still frequently recalcitrant to expression in any host system useful for an intended application. Synthetic biology app ...
... tRNA levels, and by changing the context and copy number of the gene itself (Hannig & Makrides 1998; Baneyx 1999; Aricescu et al. 2006). Despite so many options, natural genes are still frequently recalcitrant to expression in any host system useful for an intended application. Synthetic biology app ...
This article appeared in a journal published by Elsevier. The
... RNA silencing is one of the natural plant defense mechanisms against virus infection [1,2]. A current model for antiviral silencing in higher plants, taking Arabidopsis thaliana as an example, suggests that double-stranded (ds) RNA replication intermediates of viral genomic RNAs or highly structured ...
... RNA silencing is one of the natural plant defense mechanisms against virus infection [1,2]. A current model for antiviral silencing in higher plants, taking Arabidopsis thaliana as an example, suggests that double-stranded (ds) RNA replication intermediates of viral genomic RNAs or highly structured ...
The Answer Is Fifteen Percent - CHEST Journal
... variant alleles may contribute to the development of respiratory illness. These studies have not been conclusive. Because inflammatory processes are likely to be important in the pathogenesis of COPD, genetic polymorphisms that effect inflammatory mediators or the immune response may be of interest. ...
... variant alleles may contribute to the development of respiratory illness. These studies have not been conclusive. Because inflammatory processes are likely to be important in the pathogenesis of COPD, genetic polymorphisms that effect inflammatory mediators or the immune response may be of interest. ...
The liquid drop nature of nucleoli
... organized by aggregation and phase separation of their components from the surrounding cytoplasm onto a site of “nucleation”—rests on observations in the Xenopus GV. Given that chromatin organization might affect the liquid-drop behavior,4 it would be very interesting to extend these experiments to ...
... organized by aggregation and phase separation of their components from the surrounding cytoplasm onto a site of “nucleation”—rests on observations in the Xenopus GV. Given that chromatin organization might affect the liquid-drop behavior,4 it would be very interesting to extend these experiments to ...
Quantitative genetics
... under the control of many genes? In one- and two-locus models many F2 plants have phenotypes like the parental strains. Not so with 6-locus model. Just 1 in 4,096 individuals will have the genotype aabbccddeeff. ...
... under the control of many genes? In one- and two-locus models many F2 plants have phenotypes like the parental strains. Not so with 6-locus model. Just 1 in 4,096 individuals will have the genotype aabbccddeeff. ...
Introduction to Adipose Tissue Adipose tissue is not merely an
... acetyltransferase 1 (ACAT1), several phospholipase A (PLA) genes, adiponectin, the gluconeogenic enzyme PEPCK, and glycerol-3-phosphate dehydrogenase (GPD1). PPARγ also functions in macrophage lipid metabolism by inducing the expression of the macrophage scavenger receptor, CD36. The CD36 receptor i ...
... acetyltransferase 1 (ACAT1), several phospholipase A (PLA) genes, adiponectin, the gluconeogenic enzyme PEPCK, and glycerol-3-phosphate dehydrogenase (GPD1). PPARγ also functions in macrophage lipid metabolism by inducing the expression of the macrophage scavenger receptor, CD36. The CD36 receptor i ...
(2013). Nothing in genetics makes sense except in light of genomic
... this constitutes only classical evolutionary change. But if, for example, A increases by causing B to be shunted into a polar body during female meiosis and itself into the functional ovum more than 50% of the time (i.e., by meiotic drive), then this phenotype represents intraindividual genomic confl ...
... this constitutes only classical evolutionary change. But if, for example, A increases by causing B to be shunted into a polar body during female meiosis and itself into the functional ovum more than 50% of the time (i.e., by meiotic drive), then this phenotype represents intraindividual genomic confl ...
Biology of Humans 2/e
... Involves two or more genes, often on different chromosomes Many traits, including height, skin color, and eye color, vary almost continuously from one extreme to another ...
... Involves two or more genes, often on different chromosomes Many traits, including height, skin color, and eye color, vary almost continuously from one extreme to another ...
Dragon Meiosis
... simulate the process of crossing-over that occurs during prophase I. Select one sister chromatid from each of the homologous chromosomes in pair one and cut them in half. Now take each piece and tape it to the piece from the opposite chromatid. Reassemble the chromatids into the homologous chromosom ...
... simulate the process of crossing-over that occurs during prophase I. Select one sister chromatid from each of the homologous chromosomes in pair one and cut them in half. Now take each piece and tape it to the piece from the opposite chromatid. Reassemble the chromatids into the homologous chromosom ...
Trans-HHS Workshop: Diet, DNA Methylation
... provides the most compelling data for the interaction of nutrients and DNA methylation, because these dietary elements are directly involved in DNA methylation via one-carbon metabolism. The sole metabolic function of all coenzymatic forms of folate is to transfer one-carbon units. Within the scope ...
... provides the most compelling data for the interaction of nutrients and DNA methylation, because these dietary elements are directly involved in DNA methylation via one-carbon metabolism. The sole metabolic function of all coenzymatic forms of folate is to transfer one-carbon units. Within the scope ...
Genomic overview of serine proteases
... between genes are as short as 3 kb). This cluster is followed centromerically by another cluster of three proteases on 16p13.3. This cluster is, however, interrupted by two non-protease genes. Analysis of genes on both ends of each of these clusters did not reveal any sequence similarity to serine p ...
... between genes are as short as 3 kb). This cluster is followed centromerically by another cluster of three proteases on 16p13.3. This cluster is, however, interrupted by two non-protease genes. Analysis of genes on both ends of each of these clusters did not reveal any sequence similarity to serine p ...
network models for genetic testing
... DNA sequence, which carries genetic material that is found in the cells of humans and almost all other creatures [2]. While some genetic disorders may be caused by gene mutations that are inherited from the parents, other diseases are influenced by either random gene changes that occur during person ...
... DNA sequence, which carries genetic material that is found in the cells of humans and almost all other creatures [2]. While some genetic disorders may be caused by gene mutations that are inherited from the parents, other diseases are influenced by either random gene changes that occur during person ...
Modified Mendelian ratios
... Duplicate gene action • Spring growth is the result of duplicate gene action between two independent genes S and H • Spring growth: S_; H_ , S_; hh , ss; H_ • Winter growth: ss;hh • In duplicate gene action the dominant phenotype is evident when at least one dominant allele is present at each locus, ...
... Duplicate gene action • Spring growth is the result of duplicate gene action between two independent genes S and H • Spring growth: S_; H_ , S_; hh , ss; H_ • Winter growth: ss;hh • In duplicate gene action the dominant phenotype is evident when at least one dominant allele is present at each locus, ...
Clinical application of High throughput sequencing (HTS) analysis
... of the List of Analysis. This explains why some disorders like Friedreich’s Ataxia (caused by triplet repeats) or Cri-du-Chat syndrome already have a HTS position although at the moment these disorders are more efficiently detected by traditional methods due to technical limitations or high costs of ...
... of the List of Analysis. This explains why some disorders like Friedreich’s Ataxia (caused by triplet repeats) or Cri-du-Chat syndrome already have a HTS position although at the moment these disorders are more efficiently detected by traditional methods due to technical limitations or high costs of ...
Divergence with Gene Flow: Models and Data
... second, that kicks in when hybrids are produced, is epistatic incompatibility between alleles that have become fixed in different populations. The flip side of the BDM model is that if hybrids are produced and are not completely sterile, then it may happen that an allele that is fixed in one population ...
... second, that kicks in when hybrids are produced, is epistatic incompatibility between alleles that have become fixed in different populations. The flip side of the BDM model is that if hybrids are produced and are not completely sterile, then it may happen that an allele that is fixed in one population ...
Applications of Genomics
... disease in many members of a single family and are known as mutations. Classic examples include hypertrophic cardiomyopathy and Marfan syndrome. There are common variants (>1% of the general population) that have a small effect on the function of a gene. These variants do not change gene activity en ...
... disease in many members of a single family and are known as mutations. Classic examples include hypertrophic cardiomyopathy and Marfan syndrome. There are common variants (>1% of the general population) that have a small effect on the function of a gene. These variants do not change gene activity en ...
lac
... mutated, will grow on lactose. • However they make β-galactosidase all of the time. These mutants that have lost the ability to control gene expression are called constitutive mutants. They are also recessive. ...
... mutated, will grow on lactose. • However they make β-galactosidase all of the time. These mutants that have lost the ability to control gene expression are called constitutive mutants. They are also recessive. ...