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Use of lac regulatory elements for gene expression in
Use of lac regulatory elements for gene expression in

... generations in liquid medium without antibiotic. Colonies that had undergone a second recombination event (through the cloned lacG fragment) suffered the excision of the vector giving rise to Erms Lac+ colonies which had integrated the ilvBN genes in the lac operon. A double recombinant (Erms and La ...
Petunia Ap2-like Genes and Their Role in Flower and
Petunia Ap2-like Genes and Their Role in Flower and

... (A) Bright-field photograph of the top of the inflorescence with a transverse section through the inflorescence meristem and a stage 4 to 5 flower and an oblique section through a stage 6 flower and part of a stage 7 flower. (B) Dark-field photograph of a section like that shown in (A) with PhAp2A e ...
NCEA Level 1 Science (90948) 2013
NCEA Level 1 Science (90948) 2013

... resulting offspring will have two alleles – they may inherit two alleles the same, homozygous, and show that characteristic or they may inherit one of each allele, heterozygous in which case they will show the dominant allele in their phenotype. • Genetic variation: variety within a population, eg d ...
Animal Behaviour SPECIAL ISSUE: KIN SELECTION
Animal Behaviour SPECIAL ISSUE: KIN SELECTION

... expression analyses could identify shared molecular functions or biological processes that are repeatedly associated with a behavioural phenotype, and inferences at this level may also provide evidence for a behavioural toolkit. For example, in the honeybee, transcriptomic analyses with microarrays ...
Lab 3
Lab 3

... table_ALL_AML_samples.txt data_set_ALL_AML_train.txt data_set_ALL_AML_independent.txt and can be found here: http://www.kdnuggets.com/data_mining_course/data/ALL_AML_original_data.zip The cleaning involved removing the control genes and the call fields and changing the “Gene.Accession.Number” attrib ...
The Incompatible Desiderata of Gene Cluster Properties
The Incompatible Desiderata of Gene Cluster Properties

... constrain the local density of r-window clusters without also further constraining the maximum cluster length. This trade-off between global and local density gives a simple illustration of how it can be difficult to design a cluster definition that satisfies our basic intuitions about cluster prope ...
Mendel`s Experiments
Mendel`s Experiments

... Mendel thought that there must be something in each plant that controlled its traits. He called these things “factors” but we now call them genes. We also now know that each characteristic (like flower color) is controlled by two genes—one gene is inherited from each parent. The F1 plants have 1 dom ...
מצגת של PowerPoint - Tel Aviv University
מצגת של PowerPoint - Tel Aviv University

... Unless the new function can be acquired through one or a few nucleotide substitutions, it is more than likely that the copy will become a pseudogene rather than a new functional gene. ...
meiosis_text_book
meiosis_text_book

A process for analysis of microarray comparative genomics
A process for analysis of microarray comparative genomics

... the microarray slide. If a gene is conserved in both samples the fluorescence emitted at the corresponding position on the array will be a mixture derived from both labels. If the gene is present in only one sample then only one type of fluorescence will be observed. The use of a reference sample in ...
Brooker Chapter 8
Brooker Chapter 8

... corresponds to the region of the X chromosome where the bar allele is located. This bar allele is found within the region designated 16A Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
Evaluation of the Y-Chromosome Structure
Evaluation of the Y-Chromosome Structure

... gene RPS4X encode for two non-identical isoforms of the ribosomal protein S4 (families of functionally related proteins that slightly differ in amino acid sequence), two Y-linked genes CYorfl15A and CYorfl15B, homologues of the X-linked gene CXorfl15 encode for the aminoand carboxy-terminal regions ...
Gene clusters for β-lactam antibiotics and control of their expression
Gene clusters for β-lactam antibiotics and control of their expression

... However, mutations disrupting cefG do not lead to methionine auxotrophy, which suggests that there is a separate met2 in Acremonium chrysogenum. A detailed phylogenetic analysis of the CefG protein revealed that it is, indeed, a member of the serine/homoserine acetyl transferase class. However, it s ...
Correlation between sequence divergence and polymorphism
Correlation between sequence divergence and polymorphism

... population plastid sequencing (Table 1). These populations were chosen because they span the geographic range of C. americanum and were known to differ genetically based on sequencing of individual chloroplast loci (Barnard-Kubow et al., unpublished results). VA individuals were transplanted from th ...
Full text in pdf - International Microbiology
Full text in pdf - International Microbiology

... However, mutations disrupting cefG do not lead to methionine auxotrophy, which suggests that there is a separate met2 in Acremonium chrysogenum. A detailed phylogenetic analysis of the CefG protein revealed that it is, indeed, a member of the serine/homoserine acetyl transferase class. However, it s ...
The Complete Chloroplast and Mitochondrial DNA Sequence of
The Complete Chloroplast and Mitochondrial DNA Sequence of

... for land plants have a very conserved genome size, usually around 150 kb covering about 70–80 genes. In contrast, the cp genomes of green algae, although having a rather similar genome size between 150 and 200 kb, show a tremendous variation in gene content, due to massive gene loss, genome erosion, ...
Duplication of an approximately 1.5 Mb DNA segment
Duplication of an approximately 1.5 Mb DNA segment

... the distal end of chromosome 5q was used in this assay to detect allelic changes at critical chromosome 5q22qter segment. Twenty three out of 50 patients with sporadic nonpapillary RCCs were heterozygous for the locus D5S22 and 11 tumours from these patients showed a duplication of one allele at chr ...
Inheriting two copies of mutated genes that are
Inheriting two copies of mutated genes that are

... essential, these individuals might fail to develop past fertilization, die in utero, or die later in life, depending on what life stage requires this gene. An inheritance pattern in which an allele is only lethal in the homozygous form and in which the heterozygote may be normal or have some altered ...
No Slide Title
No Slide Title

... • GUS and garlic transgenes only expressed in presence of inducer (ethanol) ...
first of Chapter 11: Gene Regulation
first of Chapter 11: Gene Regulation

... lac operon model • 2 kinds of genes: structural, regulatory elements. • Polycistronic structural genes, with promoter and operator constitute the lac operon. • Promoter mutants make no lac mRNA. • lacI gene makes a repressor, which binds to the operator. • When operator is ‘repressed’ no transcript ...
Independent Assortment of Genes
Independent Assortment of Genes

... mutant plants (A and B) that have no trichomes, and these mutants seem to be potentially useful in studying trichome development. (If they are determined by single-gene mutations, then finding the normal and abnormal function of these genes will be instructive.) Each plant was crossed with wild type ...
Somatotropic axis
Somatotropic axis

... • Mediated by Insulin-like growth factors (IGFs) – Two types • IGF-I • IGF-II ...
ppt - Chair of Computational Biology
ppt - Chair of Computational Biology

... traits have been identified, largely by positional cloning. Identification of the gene  knowledge of relevant protein(s)  often leads to understanding of the molecular and physiological basis of the disease phenotype. Successful examples in positional cloning: identifcation of genes underlying ...
Molecular Evolution of Two Linked Genes, Est-6 and Sod, in
Molecular Evolution of Two Linked Genes, Est-6 and Sod, in

... Supporting information online, Text S1. Nucleotide polymorphism There was no noticeable difference in the ratio of divergence to polymorphism K/ for the noncoding regions of the lb genes, but in the coding regions the synonymous K/ ratio was 2.0 – 2.7 times higher for lbl than for lbe (Tables 1 an ...
CHAPTER 2 Genome Sequence Acquisition and
CHAPTER 2 Genome Sequence Acquisition and

... genetic factors. Hager et al. (1998) undertook a genomewide scan in affected sib pairs to identify chromosomal regions linked to obesity in a collection of French families. Model-free multipoint linkage analyses revealed evidence for linkage to a region on 10p (MLS  4.85). Two further loci on chrom ...
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Genomic imprinting

Genomic imprinting is the epigenetic phenomenon by which certain genes are expressed in a parent-of-origin-specific manner. If the allele inherited from the father is imprinted, it is thereby silenced, and only the allele from the mother is expressed. If the allele from the mother is imprinted, then only the allele from the father is expressed. Forms of genomic imprinting have been demonstrated in fungi, plants and animals. Genomic imprinting is a fairly rare phenomenon in mammals; most genes are not imprinted.In insects, imprinting affects entire chromosomes. In some insects the entire paternal genome is silenced in male offspring, and thus is involved in sex determination. The imprinting produces effects similar to the mechanisms in other insects that eliminate paternally inherited chromosomes in male offspring, including arrhenotoky.Genomic imprinting is an inheritance process independent of the classical Mendelian inheritance. It is an epigenetic process that involves DNA methylation and histone methylation without altering the genetic sequence. These epigenetic marks are established (""imprinted"") in the germline (sperm or egg cells) of the parents and are maintained through mitotic cell divisions in the somatic cells of an organism.Appropriate imprinting of certain genes is important for normal development. Human diseases involving genomic imprinting include Angelman syndrome and Prader–Willi syndrome.
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