Introduction to super-resolution microscopy
... These localization-based super-resolution techniques were introduced by three different groups in 2006. These microscopes are termed photoactivation localization microscopy (PALM) [3], fluorescence photoactivation localization microscopy (FPALM) [4] and stochastic optical reconstruction microscopy (S ...
... These localization-based super-resolution techniques were introduced by three different groups in 2006. These microscopes are termed photoactivation localization microscopy (PALM) [3], fluorescence photoactivation localization microscopy (FPALM) [4] and stochastic optical reconstruction microscopy (S ...
Chapter 25
... The cornea and lens do not have sufficient focusing power to bring nearby objects into focus on the retina Condition can be corrected with converging lenses ...
... The cornea and lens do not have sufficient focusing power to bring nearby objects into focus on the retina Condition can be corrected with converging lenses ...
lab sheet - Faculty of Engineering
... 1) The polarization direction is defined as the alignment of the electric field, which automatically sets the direction of the perpendicular magnetic field. 2) Light waves with their electric fields in the same plane are linearly polarized. 3) Polarization is measured by passing light through a pola ...
... 1) The polarization direction is defined as the alignment of the electric field, which automatically sets the direction of the perpendicular magnetic field. 2) Light waves with their electric fields in the same plane are linearly polarized. 3) Polarization is measured by passing light through a pola ...
Urban - TEM aberration correction review
... the refraction power of a real lens increasing with the angle that the beams entering the lens make with its optical axis. As a result, the electrons that scattered in the specimen at high angles come to a focus some distance in front of the Gaussian image plane that is defined by the low-angle beam ...
... the refraction power of a real lens increasing with the angle that the beams entering the lens make with its optical axis. As a result, the electrons that scattered in the specimen at high angles come to a focus some distance in front of the Gaussian image plane that is defined by the low-angle beam ...
Inexpensive optical tweezers for undergraduate laboratories
... In this section, detailed selection criteria for the components of the tweezers will be discussed. With other microscopes, lasers, or wavelengths, the selection criteria will remain the same, but the specific values for the required components may be different. A. Laser There are four important char ...
... In this section, detailed selection criteria for the components of the tweezers will be discussed. With other microscopes, lasers, or wavelengths, the selection criteria will remain the same, but the specific values for the required components may be different. A. Laser There are four important char ...
may10-94 as a Word 6.0 doc - Lyle School of Engineering
... 2) Some of the multiple choice questions may have more than one correct answer listed. If so, circle all of the correct responses for the question. 3) Please print your name at the bottom of the remaining pages. 4) The last three pages of this exam contain supplementary figures and information that ...
... 2) Some of the multiple choice questions may have more than one correct answer listed. If so, circle all of the correct responses for the question. 3) Please print your name at the bottom of the remaining pages. 4) The last three pages of this exam contain supplementary figures and information that ...
Acknowledgments
... optics is to learn how to use optical techniques for making measurements. Optical techniques are widely used in many areas of the thermal sciences for measuring system temperatures, velocities, and species on a time- and space-resolved basis. In many cases these non-intrusive optical devices have si ...
... optics is to learn how to use optical techniques for making measurements. Optical techniques are widely used in many areas of the thermal sciences for measuring system temperatures, velocities, and species on a time- and space-resolved basis. In many cases these non-intrusive optical devices have si ...
Optoniks
... OPTONIKS has completed a 3D profilometry system prototype for Intel metrology lab which is responsible for measurement requirements and requests from other departments within Intel and also develops and assesses new metrology systems at Intel. Optonik’s next step is to launch development of more 3D ...
... OPTONIKS has completed a 3D profilometry system prototype for Intel metrology lab which is responsible for measurement requirements and requests from other departments within Intel and also develops and assesses new metrology systems at Intel. Optonik’s next step is to launch development of more 3D ...
Optical Telescopes
... This occasion is now almost forgotten, because no inventions were made but a Dutchman. His device was not used for astronomical purposes, and it found its application in military use. The event, which remains in people memories, is the Galilean invention of his first telescope in 1609. The first Gal ...
... This occasion is now almost forgotten, because no inventions were made but a Dutchman. His device was not used for astronomical purposes, and it found its application in military use. The event, which remains in people memories, is the Galilean invention of his first telescope in 1609. The first Gal ...
High-precision, high-accuracy ultralong-range swept
... Reducing the speed and sweep range enables meterdepth-range measurements (configuration C in Table 1). In this case, the trigger, OCT signal, and calibrating (MZI) signal were acquired using a 1 GHz oscilloscope. An optical fiber patchcord was inserted in one arm of a second MZI interferometer and i ...
... Reducing the speed and sweep range enables meterdepth-range measurements (configuration C in Table 1). In this case, the trigger, OCT signal, and calibrating (MZI) signal were acquired using a 1 GHz oscilloscope. An optical fiber patchcord was inserted in one arm of a second MZI interferometer and i ...
Wave Optics Theory and 3-D Deconvolution for the Light Field
... the native image plane as shown in Fig. 2(a). Light field imaging can be performed using any microscope objective so long as the f-number of the microlens array is matched to the numerical aperture of the objective [1]. The camera sensor is placed behind the lenslets at a distance of one lenslet foc ...
... the native image plane as shown in Fig. 2(a). Light field imaging can be performed using any microscope objective so long as the f-number of the microlens array is matched to the numerical aperture of the objective [1]. The camera sensor is placed behind the lenslets at a distance of one lenslet foc ...
A new optical configuration in speckle interferometry for contouring
... configuration. Unlike the Leendertz configuration, the proposed configuration uses single beam illumination and dual directions of observation but the sensitivity is large and is equal to that of Leendertz’s method. In this configuration an object point is viewed symmetrically with respect to the su ...
... configuration. Unlike the Leendertz configuration, the proposed configuration uses single beam illumination and dual directions of observation but the sensitivity is large and is equal to that of Leendertz’s method. In this configuration an object point is viewed symmetrically with respect to the su ...
Imaging
... Each CCD is divided into many groups of electrodes called pixels. The exact number of pixels depends on the individual pixel size and the cost of the array. Typically, a low-cost commercial array has dimensions of 600 x 300 pixels, while high cost arrays for scientific applications have dimensions c ...
... Each CCD is divided into many groups of electrodes called pixels. The exact number of pixels depends on the individual pixel size and the cost of the array. Typically, a low-cost commercial array has dimensions of 600 x 300 pixels, while high cost arrays for scientific applications have dimensions c ...
Wave optics theory and 3-D deconvolution
... optical signal below the Shannon-Nyquist limit, causing high frequency features to be aliased in the light field. In conventional imaging, such aliasing is undesirable because it irreversibly corrupts the recorded image. However, in light field imaging, aliasing is actually beneficial. In particula ...
... optical signal below the Shannon-Nyquist limit, causing high frequency features to be aliased in the light field. In conventional imaging, such aliasing is undesirable because it irreversibly corrupts the recorded image. However, in light field imaging, aliasing is actually beneficial. In particula ...
Improving fluorescence detection in lab on chip devices
... more interference filters and a dichroic mirror. Light emitted by the fluorophore is collected by the same microscope objective and sent to a detector, passing through the dichroic and (an)other filter(s) that exclude the excitation light. Common detectors include photomultiplier tubes (PMT) and avalan ...
... more interference filters and a dichroic mirror. Light emitted by the fluorophore is collected by the same microscope objective and sent to a detector, passing through the dichroic and (an)other filter(s) that exclude the excitation light. Common detectors include photomultiplier tubes (PMT) and avalan ...
Brightfield Contrasting Techniques
... • Shade off is caused by greater diffraction at edges of objects than their centers ...
... • Shade off is caused by greater diffraction at edges of objects than their centers ...
Inverse Design of Optical Antennas for Sub-Wavelength Energy Delivery
... Assisted Magnetic Recording (HAMR). This data-recording scheme relies on focusing optical energy to locally heat the area of a single datum, several hundred square nanometers on the hard disk. In order to write data with a scan rate of up to 10 m/s, the optical system must heat the media by 200 K wi ...
... Assisted Magnetic Recording (HAMR). This data-recording scheme relies on focusing optical energy to locally heat the area of a single datum, several hundred square nanometers on the hard disk. In order to write data with a scan rate of up to 10 m/s, the optical system must heat the media by 200 K wi ...
Nanofabrication via Atom Optics with Chromium
... nulling of the residual magnetic field. Fig. 4 shows a sample created with slightly sub-optimal cooling, so that the additional lines are more clearly distinguishable. ...
... nulling of the residual magnetic field. Fig. 4 shows a sample created with slightly sub-optimal cooling, so that the additional lines are more clearly distinguishable. ...
Optical Activity
... are optically active, including all amino acids. Specific rotation (also called rotary power) is defined to be the actual rotation per unit concentration and per unit length for solutions of the pure material. Specific rotations are published for many optically active substances at various temperatu ...
... are optically active, including all amino acids. Specific rotation (also called rotary power) is defined to be the actual rotation per unit concentration and per unit length for solutions of the pure material. Specific rotations are published for many optically active substances at various temperatu ...
Looking through walls and around corners with
... after displaying the correct phase-pattern, one can image an object located in the three-dimensional volume of FOV×FOV×∆zFOV around the corrected point-source. The transverse resolution of the scattering-microscope at the object plane, δx, is dictated by the ...
... after displaying the correct phase-pattern, one can image an object located in the three-dimensional volume of FOV×FOV×∆zFOV around the corrected point-source. The transverse resolution of the scattering-microscope at the object plane, δx, is dictated by the ...
An Optical ‘‘Janus’’ Device for Integrated Photonics By Xiang Zhang*
... opens the possibility to precisely control the flow of light in a medium and thus allow to design novel devices for microwave and optical frequencies such as invisibility cloaks,[6–10] light concentrators,[11–15] beam manipulators,[16–19] object transformers,[20,21] and even electromagnetic wormhole ...
... opens the possibility to precisely control the flow of light in a medium and thus allow to design novel devices for microwave and optical frequencies such as invisibility cloaks,[6–10] light concentrators,[11–15] beam manipulators,[16–19] object transformers,[20,21] and even electromagnetic wormhole ...
Unit 3 Cells and the Microscope
... 5. Arm-part of the microscope that you carry the microscope with. 6. Coarse Adjustment Knob-large, round knob on the side of the microscope used for focusing the specimen; it may move either the stage or the upper part of the microscope. 7. Fine Adjustment Knob-small, round knob on the side of the m ...
... 5. Arm-part of the microscope that you carry the microscope with. 6. Coarse Adjustment Knob-large, round knob on the side of the microscope used for focusing the specimen; it may move either the stage or the upper part of the microscope. 7. Fine Adjustment Knob-small, round knob on the side of the m ...
Imaging visible light using anisotropic metamaterial slab lens Jie Yao, Kun-Tong Tsai,
... Abstract: It has been shown that an anisotropic metamaterial made of nanowire array can realize negative refraction of light even without a negative phase index of refraction. Such non-resonant bulk material can be fabricated by bottom-up electrochemical method. Using this material, we were able to ...
... Abstract: It has been shown that an anisotropic metamaterial made of nanowire array can realize negative refraction of light even without a negative phase index of refraction. Such non-resonant bulk material can be fabricated by bottom-up electrochemical method. Using this material, we were able to ...
Synopses by Kim Larsen
... Since high powered laser systems will not just heat windows, but also other optical elements, similar derivations have been done to determine other system design choices. If the laser is not pulsed, but a CW laser, thermal distortion even with the best materials will be too high, a system involving ...
... Since high powered laser systems will not just heat windows, but also other optical elements, similar derivations have been done to determine other system design choices. If the laser is not pulsed, but a CW laser, thermal distortion even with the best materials will be too high, a system involving ...
In this lab you will use the phenomenon of interference... thickness of thin films. Two interference techniques, Michelson and... Thin Film Measurement 1 Introduction
... Two measuring techniques are used and compared with this set-up. The first involves using the micrometer on the mirror to measure the difference in zero path length between the thin film and no film. The second technique uses the step height change in the interference pattern much like the Fizeau te ...
... Two measuring techniques are used and compared with this set-up. The first involves using the micrometer on the mirror to measure the difference in zero path length between the thin film and no film. The second technique uses the step height change in the interference pattern much like the Fizeau te ...
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.