Ultra–large field-of-view two-photon microscopy
... The design of the scan system begins with the selection of the objective. We seek a field-ofview that encompasses 10 mm, a numerical aperture (NA) to achieve 1-µm resolution, and a back aperture that does not exceed 25 mm. The singular choice is a 4-times magnification (f = 45 mm), 0.28 NA air-immer ...
... The design of the scan system begins with the selection of the objective. We seek a field-ofview that encompasses 10 mm, a numerical aperture (NA) to achieve 1-µm resolution, and a back aperture that does not exceed 25 mm. The singular choice is a 4-times magnification (f = 45 mm), 0.28 NA air-immer ...
Chester F - RIT Center for Imaging Science
... a) To arrive at the correct solution the student must first note which sources are coherent. Light from each beam falls on each of the two slits, and so the light from each beam emerging from the two slits is coherent. But the two beams are not coherent sources with respect to each other. So we have ...
... a) To arrive at the correct solution the student must first note which sources are coherent. Light from each beam falls on each of the two slits, and so the light from each beam emerging from the two slits is coherent. But the two beams are not coherent sources with respect to each other. So we have ...
Technology Integration for Analysis of High Throughput Cellular
... Cytomics is going to be important because it is the cell that is the ultimate functional endpoint. The cell is the minimal functional unit within our physiology and thus the functional unit that can be manipulated. Complexity of cell function is only part of why Cytomics will become a major field of ...
... Cytomics is going to be important because it is the cell that is the ultimate functional endpoint. The cell is the minimal functional unit within our physiology and thus the functional unit that can be manipulated. Complexity of cell function is only part of why Cytomics will become a major field of ...
Waves - Morgan Science
... Upright – image is right side up compared to object Inverted – image is upside down as compared to object ...
... Upright – image is right side up compared to object Inverted – image is upside down as compared to object ...
Lens Types
... from the object to the lens, z2 is the length from the lens to the focal point, and f is the focal length the equation to find the magnification is , M=-(z2/z1) You would place a sensor at the focal point to get a focused image Convex have a + focal length (image is on the other side of light) Conca ...
... from the object to the lens, z2 is the length from the lens to the focal point, and f is the focal length the equation to find the magnification is , M=-(z2/z1) You would place a sensor at the focal point to get a focused image Convex have a + focal length (image is on the other side of light) Conca ...
A History of Imaging
... Fraunhofer’s work in turn prompted the British Royal Society to support a similar British effort on glass. British support of Faraday led to further improvements in glass homogeneity. However, Faraday did little to advance the link between glass composition and its optical properties. Instead, advanc ...
... Fraunhofer’s work in turn prompted the British Royal Society to support a similar British effort on glass. British support of Faraday led to further improvements in glass homogeneity. However, Faraday did little to advance the link between glass composition and its optical properties. Instead, advanc ...
TO THE POSSIBILITY OF CALCULATION
... numbers at these frequencies. This fact poses serious constrains to the possibilities of the imaging systems that use them. Many times, the system has to include some kind of moving mirrors in order to scan the entire vision field. These limitations boost research towards finding alternative systems ...
... numbers at these frequencies. This fact poses serious constrains to the possibilities of the imaging systems that use them. Many times, the system has to include some kind of moving mirrors in order to scan the entire vision field. These limitations boost research towards finding alternative systems ...
Undecided still
... Registration and mosaicing Segmentation, tracing and tracking Classification and clustering Modeling ...
... Registration and mosaicing Segmentation, tracing and tracking Classification and clustering Modeling ...
Advantages of Holographic Optical Tweezers
... Stimulated by the work in the area of cooling atoms the first schemes of using light for trapping microscopic particles were used in the 1970s?, ? . In most of these experiments two laser beams were used to accomplish a stable trap. In 1986 Ashkin et al.? realized a single beam gradient force optica ...
... Stimulated by the work in the area of cooling atoms the first schemes of using light for trapping microscopic particles were used in the 1970s?, ? . In most of these experiments two laser beams were used to accomplish a stable trap. In 1986 Ashkin et al.? realized a single beam gradient force optica ...
PPT
... thereby change how much light exits the bottom port? Rotate the entire interferometer (in the plane of the paper). For example, if we rotate it clockwise, the light making the clockwise circuit will have farther to go (the beamsplitter is “running away”), while the counterclockwise path will be shor ...
... thereby change how much light exits the bottom port? Rotate the entire interferometer (in the plane of the paper). For example, if we rotate it clockwise, the light making the clockwise circuit will have farther to go (the beamsplitter is “running away”), while the counterclockwise path will be shor ...
Introduction to Fiber Optics
... travels in straight lines, so it is no problem. What if the hallway has a bend in it? You could place a mirror at the bend to reflect the light beam around the corner. What if the hallway is very winding with multiple bends? You might line the walls with mirrors and angle the beam so that it bounces ...
... travels in straight lines, so it is no problem. What if the hallway has a bend in it? You could place a mirror at the bend to reflect the light beam around the corner. What if the hallway is very winding with multiple bends? You might line the walls with mirrors and angle the beam so that it bounces ...
Coherent Optical Information Systems
... bate at ⫽ 514 nm (19). The maximum reflecoccurs as different portions of this broad specavailability of coherent light sources has been tivity is at the Bragg wavelength ⫽ 1550 nm trum reach the destination with different delays. the key innovation enabling the rapid rise of (Fig. 4). Notice tha ...
... bate at ⫽ 514 nm (19). The maximum reflecoccurs as different portions of this broad specavailability of coherent light sources has been tivity is at the Bragg wavelength ⫽ 1550 nm trum reach the destination with different delays. the key innovation enabling the rapid rise of (Fig. 4). Notice tha ...
PH 481 - Physics | Oregon State University
... In order to do this and to find the Brewster angle B, rotate the reflector so that the incident angle i ≈ 55˚ and view the reflected beam on a card. Adjust the polarizer so as to minimize the brightness of the reflected beam. Change the angle of incidence and plane of polarization alternately to a ...
... In order to do this and to find the Brewster angle B, rotate the reflector so that the incident angle i ≈ 55˚ and view the reflected beam on a card. Adjust the polarizer so as to minimize the brightness of the reflected beam. Change the angle of incidence and plane of polarization alternately to a ...
1 Introduction 2 Theory of Optical Trapping
... Figure 2 Schematic overview of the undergraduate optical tweezers setup at LION. A laser beam (λ = 658 nm, 65 mW) is sent into the objective with a dichroic mirror. A steering lens placed at the right position, allows for the manipulation of the trap position, while overfilling the back aperture of ...
... Figure 2 Schematic overview of the undergraduate optical tweezers setup at LION. A laser beam (λ = 658 nm, 65 mW) is sent into the objective with a dichroic mirror. A steering lens placed at the right position, allows for the manipulation of the trap position, while overfilling the back aperture of ...
All Optical Networks
... Current fiber networks use electronic switching and are therefore limited to electronic speeds of a few gigabits per second. For higher speeds, it is important that the signal remain photonic throughout its path. Such networks, which use optical switching and routing, are called all-optical. ...
... Current fiber networks use electronic switching and are therefore limited to electronic speeds of a few gigabits per second. For higher speeds, it is important that the signal remain photonic throughout its path. Such networks, which use optical switching and routing, are called all-optical. ...
In the diagram below, the optical train of a set of binoculars is found
... The Fraunhofer diffraction pattern from two microscopic circular holes separated by a distance d is observed at a wavelength of 500 nm on a screen placed 50 cm from the aperture plane. Inspection of the pattern shows that it is at the Rayleigh limit of resolution (i.e. the principal maximum appears ...
... The Fraunhofer diffraction pattern from two microscopic circular holes separated by a distance d is observed at a wavelength of 500 nm on a screen placed 50 cm from the aperture plane. Inspection of the pattern shows that it is at the Rayleigh limit of resolution (i.e. the principal maximum appears ...
Chapter 25
... The cornea and lens do not have sufficient focusing power to bring nearby objects into focus on the retina Condition can be corrected with converging lenses ...
... The cornea and lens do not have sufficient focusing power to bring nearby objects into focus on the retina Condition can be corrected with converging lenses ...
Microscope Lab
... 1. Start with the scanning objective in place and the stage all the way down. 2. Obtain a blank microscope slide and a permanent marker from the materials table 3. Using the mm side of your ruler, use the permanent marker to mark 5 dots on the blank slide 1mm apart. (try and stay towards the center ...
... 1. Start with the scanning objective in place and the stage all the way down. 2. Obtain a blank microscope slide and a permanent marker from the materials table 3. Using the mm side of your ruler, use the permanent marker to mark 5 dots on the blank slide 1mm apart. (try and stay towards the center ...
Document
... cell of 10-liters volume. The order in which the different images of the slit were formed is shown in each case by the numbers beside them, although the decrease in intensity of the beams can be used as an equally good indication. Figure 2 shows the simplest possible case with one reflection each fr ...
... cell of 10-liters volume. The order in which the different images of the slit were formed is shown in each case by the numbers beside them, although the decrease in intensity of the beams can be used as an equally good indication. Figure 2 shows the simplest possible case with one reflection each fr ...
From near-field optics to optical antennas
... Typically, the probe is engineered to exploit the unique tion, Charles Carniglia and Leonard Mandel at the University properties of metal nanostructures at optical frequencies to of Rochester showed that the incident wave, reflected wave, localize incident radiation and enhance the light–matter in- ...
... Typically, the probe is engineered to exploit the unique tion, Charles Carniglia and Leonard Mandel at the University properties of metal nanostructures at optical frequencies to of Rochester showed that the incident wave, reflected wave, localize incident radiation and enhance the light–matter in- ...
Optimizing Fluorescence Signal Quality
... fluorescence light intensities, thereby allowing the calculation of fluorescence ratios without interpolated values. Live Count: the light intensity observed by the photomultiplier tube. ...
... fluorescence light intensities, thereby allowing the calculation of fluorescence ratios without interpolated values. Live Count: the light intensity observed by the photomultiplier tube. ...
Red Tide Specifications
... OFLV Variable Longpass Order-sorting Filters block second- and third-order light. These filters are optional. ...
... OFLV Variable Longpass Order-sorting Filters block second- and third-order light. These filters are optional. ...
EEE440 Modern Communication Systems Optical Fibre
... – LED has an incoherent optical output whereas Laser produces highly coherent, monochromatic and directional output because a cavity exist for wavelength selectivity ...
... – LED has an incoherent optical output whereas Laser produces highly coherent, monochromatic and directional output because a cavity exist for wavelength selectivity ...
Microfluidic Optical Devices - Bilkent University Mechanical
... successfully performed sample injection, single-file flow through the detection system and sorting of fluorescent microbeads. They could not achieve fully autonomous cell sorting, but they indicated that as a future direction. They also demonstrated the feasibility of high-gain avalanche photodiodes ...
... successfully performed sample injection, single-file flow through the detection system and sorting of fluorescent microbeads. They could not achieve fully autonomous cell sorting, but they indicated that as a future direction. They also demonstrated the feasibility of high-gain avalanche photodiodes ...
Development of a Total Internal Reflection Illumination System for
... Many cell surface receptors consist of multiple subunits that non-‐covalently associate to form a complete receptor. The integrin receptor is one such example. It is hypothesized that a drug (Thioridazine) ...
... Many cell surface receptors consist of multiple subunits that non-‐covalently associate to form a complete receptor. The integrin receptor is one such example. It is hypothesized that a drug (Thioridazine) ...
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.