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Anti-Chromogranin A antibody ab45179 Product datasheet 9 Abreviews 8 Images
Anti-Chromogranin A antibody ab45179 Product datasheet 9 Abreviews 8 Images

... Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Chromogranin A aa 1-100 (N terminal). ...
Chapter 5 part II
Chapter 5 part II

... • Direct labeling – to label the test samples directly with a fluorescent dye and then detect the labeled molecules that bind to the proteins of a microarray with a laser scanner. Two-dye strategy (e.g. Cy3 or Cy5) can be used to compare proteins in two different sample on a single array. • Sandwich ...
Ingested protein dose response of muscle and albumin protein
Ingested protein dose response of muscle and albumin protein

... RESULTS Direct correlation of increased dietary protein and MPS and APS up until 20 grams of ingested egg protein.  At 40 grams, no change in MPS or APS, however there was greater measure of leucine oxidation. ...
Resolvigen 3
Resolvigen 3

... When antibodies are so weak that they fail to react with some of the homozygous test RBCs, giving what appears to be spurious reactions; in this case the most likely match is found and the related antibody specificities suggested, hinting at procedures for confirming them. Varying reaction scores of ...
Specification sheet
Specification sheet

... This antibody is intended for use to qualitatively identify Thyrotrophin (TSH) antigen by light microscopy in formalin fixed, paraffin embedded tissue sections using immunohistochemical detection methodology. Interpretation of any positive or negative staining must be complemented with the evaluatio ...
Supplementary Information
Supplementary Information

... MS/MS spectrum used for identification of Cyp18, control CoIP experiments, a further experiment for identification of the interaction of purified recombinant p53 and Cyp18 proteins by MS, p53 basic activation after temperature shift (37°C  32°C), p53 activation and p53-target gene expression after ...
Immunoanalysis - Part 1 : What are antibodies?
Immunoanalysis - Part 1 : What are antibodies?

... hybridoma. The antibodies from each hybridoma line will recognise a single epitope. The binding constants can be measured to select the most immunogenic antibodies, and the IgG subclass selected by purification. The selected hybridoma may be cultured indefinitely. This gives monoclonal antibodies an ...
BIOT 184 Introduction to Biotechnology
BIOT 184 Introduction to Biotechnology

... to the matrix, through the use of a colorimetric substrate. Major advantages of this technique are that the antigen does not need to be purified prior to use, and that these assays are very specific. However, one disadvantage is that not all antibodies can be used. Monoclonal antibody combinations m ...
Protein A CIP Resin
Protein A CIP Resin

... Protein A based affinity chromatography is the process scale capture step for antibodies due to the excellent selectivity and suitability for platform separations. There are a few factors of concern when using protein A resins for process scale antibody purification, with the major issue being the h ...
protein quality and quantity
protein quality and quantity

... made. There are nine amino acids that are essential to human health and nutrition. All proteins have all essential amino acids, but some proteins don’t enough of certain amino acids. A food that has all the essential amino acids in the right amount that an individual needs is called a complete prote ...
ANSWER: Proteins, Amino Acids and Carbs
ANSWER: Proteins, Amino Acids and Carbs

... While this might be fine for the general population for whom soy beans are no more than an important foodstuff, this is not good enough for those using soy for the specific chemical makeup of its protein. It is very important that the soy proteins they use metabolize into the correct balance of amin ...
MONOCLONAL ANTIBODY TO HUMAN C3/C3a/C3a
MONOCLONAL ANTIBODY TO HUMAN C3/C3a/C3a

... The monoclonal antibody 2898 recognizes the C-terminus of human complement protein C3a. The antibody recognizes intact C3, C3a as well as C3a-desArg. C3 is the most abundant protein of the complement system with serum protein levels of about 1.3 mg/ml. The complement system is an important part of t ...
Self-association of the SET domains of human ALL-1 and of
Self-association of the SET domains of human ALL-1 and of

... plasmids encoding appropriate partners were transiently cotransfected into COS cells. The proteins produced were examined for co-precipitation by Western blotting. In each case, signi®cant co-precipitation was observed (Figure 3). To further test the ability of the SET domain to self associate in vi ...
Document
Document

... • Horizontal Agarose Gels • Agarose forms a gel or molecular sieve that supports the movement of small materials in solution used for DNA • Vertical Polyacrylamide Gels ...
Monoclonal Antibodies - Kenan Fellows Program
Monoclonal Antibodies - Kenan Fellows Program

... The resulting cell is called a hybridoma. Each hybridoma produces large quantities of identical antibody molecules. By allowing the hybridoma to multiply in culture, it is possible to produce a population of cells, each of which produces identical antibody molecules. These antibodies are called "mon ...
iBind™ Western Device— enjoy hands-free western
iBind™ Western Device— enjoy hands-free western

... Figure 1. Western membranes processed on the iBindTM device show superior sensitivity as compared to western membranes processed manually. A) A western membrane with recombinant interferon-a (left to right: 50ng-6ng purified protein load) was processed on the iBindTM device or using standard manual ...
Application 1
Application 1

... 2. Proteinase 3: This is a clinically relevant biomarker used in diagnosis of the human autoimmune disease Wegener’s granulomatosis. The antigen is immobilized onto the gold-coated array surface and probed with serum sample containing the corresponding autoantibody. ...
Datasheet for Protein Marker, Broad Range (2-212 kDa)
Datasheet for Protein Marker, Broad Range (2-212 kDa)

... Description: Protein Marker, Broad Range is a mixture of purified proteins with known amino acid sequences. They are resolved to 13 sharp bands when analyzed by SDS-PAGE (Tris-Glycine) and stained with Coomassie Blue R-250 (1). Two bands (BSA, MW 66.4 kDa and Triosephosphate isomerase, MW 27.0 kDa) ...
PepIDENT — bio-peptide Library Expression System for Epitope
PepIDENT — bio-peptide Library Expression System for Epitope

... The pattern design can be explained like in case the recognition of an epitope by an antibody paratope needs two parts of text located on two different pages e.g. the bottom lines of the one page and the top lines of the next page the specific recognition can only be realized if there are overlappin ...
alborz-final
alborz-final

... strands many times so that a high concentration of sample cDNA is available [7]. This sample cDNA is then washed over the surface of the microarray and complementary strands attach. Since florescent nucleotides were used in the sample, the matching pairs can be detected using laser [11]. For a more ...
File
File

... An antigen is a protein on the outside of a foreign cell that can be recognised as ‘foreign’ and can evoke an immune response A protein produced by certain leucocytes (B lymphocytes, plasma cells) in response to a specific antigen In the bone marrow Antibodies are produced by lymphocytes once they h ...
MJFF Alpha-Synuclein Protein Request Form
MJFF Alpha-Synuclein Protein Request Form

... I agree that pursuant to MJFF's agreement with Proteos, the supplier of Parkinson’s disease-relevant proteins, these proteins may be used only for research purposes, and any commercial use of these proteins requires separate written agreement with the supplier and MJFF. I agree that the proteins wil ...
New construction kit for designing new proteins
New construction kit for designing new proteins

... structural fragments as possible. She also wants to make the database available to everybody, which is why it has to have a web-based design. In addition, the researchers are continually switching between laboratory work and computers, as all theoretical findings need to be confirmed experimentally ...
Support vector machines for protein function prediction
Support vector machines for protein function prediction

... Functional Classification of Proteins by SVM What is SVM? • Support vector machines, a machine learning method, learning by examples, statistical learning, classify objects into one of the two classes. Advantages of SVM: • Diversity of class members (no racial discrimination). • Use of sequence-der ...
Lecture 11, chemical genetics - Cal State LA
Lecture 11, chemical genetics - Cal State LA

... (the protein shell encasing the viral genome) In other words, the attached protein encoded by the cDNA clone is presented on the outer surface of each phage particle ...
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Immunoprecipitation

Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure.
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