Eukaryotic Transcription factors: the DNA binding domain
... groove of DNA and the other lies at an angle across DNA. Two adjacent α-helices separated by a "turn" of several amino acids enables the protein to bind to DNA . However, the HTH motif cannot fold or function alone, but is always part of a larger DNA-binding domain and amino acid residues outside th ...
... groove of DNA and the other lies at an angle across DNA. Two adjacent α-helices separated by a "turn" of several amino acids enables the protein to bind to DNA . However, the HTH motif cannot fold or function alone, but is always part of a larger DNA-binding domain and amino acid residues outside th ...
Amino acid and Protein
... The genetic code is used universally in all present-day organisms, with some exceptions ...
... The genetic code is used universally in all present-day organisms, with some exceptions ...
Powerpoint file
... Gated Diffusion Barrier Model of Nuclear Transport Meshwork of disordered protein domains containing FG repeats ...
... Gated Diffusion Barrier Model of Nuclear Transport Meshwork of disordered protein domains containing FG repeats ...
Gene7-26
... inhibit target proteins G proteins are guanine nucleotide-binding proteins. Trimeric G proteins are associated with the plasma membrane. When bound by GDP the trimer remains intact and is inert. When the GDP is replaced by GTP, the a subunit is released from the bg dimer. Either the a monomer or the ...
... inhibit target proteins G proteins are guanine nucleotide-binding proteins. Trimeric G proteins are associated with the plasma membrane. When bound by GDP the trimer remains intact and is inert. When the GDP is replaced by GTP, the a subunit is released from the bg dimer. Either the a monomer or the ...
Introduction
... • Protein interaction: linear and diffusion kernels – Using a database of known interactions (von Mering et al., 2002) – Linear kernel function for interaction matrix – Diffusion kernel: Graphs -> kernel matrix • Based on a random walk on the graph • Nodes that are connected by shorter paths or by m ...
... • Protein interaction: linear and diffusion kernels – Using a database of known interactions (von Mering et al., 2002) – Linear kernel function for interaction matrix – Diffusion kernel: Graphs -> kernel matrix • Based on a random walk on the graph • Nodes that are connected by shorter paths or by m ...
emboj200852-sup
... cells (Stratagene). Cells were grown to OD600 = 0.5-0.7 and induced with 0.1 mM isopropyl -D-thiogalactopyranoside (IPTG) at 21oC for 12 h. Cell lysis, and affinity purification with glutathione-agarose beads (Sigma) were done as described previously (Reynaud et al., 2000). Fusion proteins were col ...
... cells (Stratagene). Cells were grown to OD600 = 0.5-0.7 and induced with 0.1 mM isopropyl -D-thiogalactopyranoside (IPTG) at 21oC for 12 h. Cell lysis, and affinity purification with glutathione-agarose beads (Sigma) were done as described previously (Reynaud et al., 2000). Fusion proteins were col ...
amino acids - CRCBiologyY11
... 2. The amino acid group consisting of 2 hydrogen atoms and a nitrogen atom. 3. This type of bond is formed between the two amino acids. 4. Polymers made up of long chains of 20 different amino acids. 5. The amino acid group consisting of COOH. 6. This amino acid group varies from a single hydrogen a ...
... 2. The amino acid group consisting of 2 hydrogen atoms and a nitrogen atom. 3. This type of bond is formed between the two amino acids. 4. Polymers made up of long chains of 20 different amino acids. 5. The amino acid group consisting of COOH. 6. This amino acid group varies from a single hydrogen a ...
Cell Membrane
... It surrounds each cell and separating its contents from the extra cellular environment. It is selectively permeable and controls the passage of substances into and out of the cell. The protoplasm consists of a central more dense part called nucleus, and a peripheral less dense part called the ...
... It surrounds each cell and separating its contents from the extra cellular environment. It is selectively permeable and controls the passage of substances into and out of the cell. The protoplasm consists of a central more dense part called nucleus, and a peripheral less dense part called the ...
S•Tag : A Multipurpose Fusion Peptide for Recombinant Proteins
... for measuring protein expression in cells, since it can be applied to both soluble and insoluble proteins. Up to 10µl of a 1/100 dilution of 6M urea or guanidine HCl can be added to the assay with little effect (data not shown). Multiple samples can easily be screened for expression levels by prepar ...
... for measuring protein expression in cells, since it can be applied to both soluble and insoluble proteins. Up to 10µl of a 1/100 dilution of 6M urea or guanidine HCl can be added to the assay with little effect (data not shown). Multiple samples can easily be screened for expression levels by prepar ...
THE EFFECT OF VARIOUS ACIDS ON THE DIGESTION OF
... rate of liberation of carmine from carmine fibrin. As has been pointed out by various authors, there is considerable doubt as to whether any of these methods actually follows the chemical changes in the structure of the protein during hydrolysis. The recent improvements in the technique of the deter ...
... rate of liberation of carmine from carmine fibrin. As has been pointed out by various authors, there is considerable doubt as to whether any of these methods actually follows the chemical changes in the structure of the protein during hydrolysis. The recent improvements in the technique of the deter ...
PPT
... OK, now that we know the structures and functions inside different types of cells, now we need to know: ...
... OK, now that we know the structures and functions inside different types of cells, now we need to know: ...
Crossing Membranes 1 – Passive Processes
... membrane of larger polar molecules, such as sugars and amino acids ...
... membrane of larger polar molecules, such as sugars and amino acids ...
1+1+1 - Computer Science, Columbia University
... PSI-BLAST rank: use training sequence as query and rank testing sequences with PSI-BLAST e-value eMotif Kernel (Ben-Hur et al., 2003): features are known protein motifs, stored using trie SVM-pairwise (Liao & Noble, 2002): feature vectors of ...
... PSI-BLAST rank: use training sequence as query and rank testing sequences with PSI-BLAST e-value eMotif Kernel (Ben-Hur et al., 2003): features are known protein motifs, stored using trie SVM-pairwise (Liao & Noble, 2002): feature vectors of ...
STUDIES ON MAMMALIAN MATURATION ANTIGEN(SMA2)
... To understand the involvement of the antigens in the event of fertility as well as the cause of the infertility of male and female,the characterization of the sperm antigens and their antibodies that can be used in blocking these events are essential.The major goat sperm maturation antigen (SMA2) is ...
... To understand the involvement of the antigens in the event of fertility as well as the cause of the infertility of male and female,the characterization of the sperm antigens and their antibodies that can be used in blocking these events are essential.The major goat sperm maturation antigen (SMA2) is ...
5 Quantitative Determination of Proteins
... 3. Give examples of nitrogen-containing molecules that might be present in a protein solution. 4. Describe how the Lowry test is a modification of the biuret test. Compare the sensitivities of these two tests for proteins. 5. There are advantages and disadvantages associated with all of the quantita ...
... 3. Give examples of nitrogen-containing molecules that might be present in a protein solution. 4. Describe how the Lowry test is a modification of the biuret test. Compare the sensitivities of these two tests for proteins. 5. There are advantages and disadvantages associated with all of the quantita ...
Protein Mobility within Minireview the Nucleus
... “off” state (the “on” state defined as its higher affinity equilibrium location), such off-state complexes may themselves move. Alternatively, the off state for some proteins may consist of individual molecules moving alone. In both cases, the process can be diffusion. Moreover, if a molecule or str ...
... “off” state (the “on” state defined as its higher affinity equilibrium location), such off-state complexes may themselves move. Alternatively, the off state for some proteins may consist of individual molecules moving alone. In both cases, the process can be diffusion. Moreover, if a molecule or str ...
FT-IR Protein Structure Analyzer
... PROTA was introduced in 1998 as the first dedicated solution for structure elucidation of biologics and since has become the industry’s preferred choice. PROTA provides a fast, cost-effective and sensitive way to determine secondary structure of a protein or to follow structural changes due to pertu ...
... PROTA was introduced in 1998 as the first dedicated solution for structure elucidation of biologics and since has become the industry’s preferred choice. PROTA provides a fast, cost-effective and sensitive way to determine secondary structure of a protein or to follow structural changes due to pertu ...
Chemistry of Lipids and Proteins
... c. What is the molecular formula for the amino acid valine? C__H__O__N__ 14. Are the molecular formulas for all amino acids the same? __________________ 15. What end arrangement of atoms is present in amino acids that is also present in fatty acids? ___________________________ Another end arrangeme ...
... c. What is the molecular formula for the amino acid valine? C__H__O__N__ 14. Are the molecular formulas for all amino acids the same? __________________ 15. What end arrangement of atoms is present in amino acids that is also present in fatty acids? ___________________________ Another end arrangeme ...
HERBALIFE Protein Snacks
... vitamins. It contains 10 grams of protein to sustain your energy and satisfy your appetite, and contains also Vitamins E, B6, B12, Niacin, Thiamine and Riboflavin. There are zero trans fats, no artificial sweeteners, and has just 140 calories per bar. Protein Bar Deluxe comes in three flavors: Vanil ...
... vitamins. It contains 10 grams of protein to sustain your energy and satisfy your appetite, and contains also Vitamins E, B6, B12, Niacin, Thiamine and Riboflavin. There are zero trans fats, no artificial sweeteners, and has just 140 calories per bar. Protein Bar Deluxe comes in three flavors: Vanil ...
Lecture 16 - Biology Courses Server
... GEF in donor membrane promotes nucleotide exchange, activating Sar1 @ ER, (ARF @ Golgi) and promoting coat assembly… GTP hydrolysis serves as “timer” delaying uncoating (GAP in target membrane?)… GTPase “cycle” provides directionality to vesicle coating/uncoating ...
... GEF in donor membrane promotes nucleotide exchange, activating Sar1 @ ER, (ARF @ Golgi) and promoting coat assembly… GTP hydrolysis serves as “timer” delaying uncoating (GAP in target membrane?)… GTPase “cycle” provides directionality to vesicle coating/uncoating ...
Lecture 3
... CS (envelope) conformation of Cg-endo L-proline peptide group at the trans position with respect to Ca-H (Y=120o), as in collagene ...
... CS (envelope) conformation of Cg-endo L-proline peptide group at the trans position with respect to Ca-H (Y=120o), as in collagene ...
Understanding the complexity of Protein Function
... Background Information • Discovered over 20 years ago • Enzyme Class: Isomerase • Is a member of the cytochrome P450 enzyme family • P450s belong to a large family of proteins containing a heme cofactor • P450 enzymes have been identified in all domains of life • Prostaglandins are derivative of Ar ...
... Background Information • Discovered over 20 years ago • Enzyme Class: Isomerase • Is a member of the cytochrome P450 enzyme family • P450s belong to a large family of proteins containing a heme cofactor • P450 enzymes have been identified in all domains of life • Prostaglandins are derivative of Ar ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.