ASD v2.0: updated content and novel features
... which not only provides insight into the allosteric mechanism in various biological processes but also contributes to the explanation of some disease-associated mutations away from the orthosteric site (27). Since the release of ASD v1.0, feedback from users has led to many excellent suggestions on ...
... which not only provides insight into the allosteric mechanism in various biological processes but also contributes to the explanation of some disease-associated mutations away from the orthosteric site (27). Since the release of ASD v1.0, feedback from users has led to many excellent suggestions on ...
Purification and Characterization of Two Thermostable Proteases
... inhibitor PMSF, but not by EDTA and thiol reagents such as iodoacetamide, which was specific for true cysteine proteases. Therefore, the two proteases were classified as serine proteases. Proteases are also classified on the basis of their optimum pH of activity (e.g., acidic, neutral, or alkaline p ...
... inhibitor PMSF, but not by EDTA and thiol reagents such as iodoacetamide, which was specific for true cysteine proteases. Therefore, the two proteases were classified as serine proteases. Proteases are also classified on the basis of their optimum pH of activity (e.g., acidic, neutral, or alkaline p ...
Identification of genomic features in the classification of loss
... this paper, we propose a study that analyzes the genomic features of LoF and GoF instances to find features that can be used to classify LoF and GoF mutations. Methods: In order to collect experimentally verified LoF and GoF mutational information, we obtained 816 LoF mutations and 474 GoF mutations ...
... this paper, we propose a study that analyzes the genomic features of LoF and GoF instances to find features that can be used to classify LoF and GoF mutations. Methods: In order to collect experimentally verified LoF and GoF mutational information, we obtained 816 LoF mutations and 474 GoF mutations ...
Using Capillary Electrophoresis To Follow the Acetylation of the
... relates the electrophoretic mobility of a protein in an electrostatic field to its effective charge Zenor unperturbed charge Zp and its mass M; Cea and C p are proportionality constants for Ze, and Zp, respectively. The mass of the protein is related to the hydrodynamic drag as it moves through the ...
... relates the electrophoretic mobility of a protein in an electrostatic field to its effective charge Zenor unperturbed charge Zp and its mass M; Cea and C p are proportionality constants for Ze, and Zp, respectively. The mass of the protein is related to the hydrodynamic drag as it moves through the ...
two types of titin interactions lead to an asymmetrical sorting of actinin
... experiments in myogenic cells have demonstrated that α-actinin, lacking the C-terminal domain, is also sorted to the sarcomeric Z-disk (Schultheiss et al., 1992). This α-actinin causes Z-disk misassembly during the progression of myofibrillogenesis and the formation of Z-rods reminiscent of those fo ...
... experiments in myogenic cells have demonstrated that α-actinin, lacking the C-terminal domain, is also sorted to the sarcomeric Z-disk (Schultheiss et al., 1992). This α-actinin causes Z-disk misassembly during the progression of myofibrillogenesis and the formation of Z-rods reminiscent of those fo ...
The primary and three-dimensional structures of a nine-haem
... cannot be located in protein electron-density maps at 1.8 Å resolution, the only situation when it is possible to clearly distinguish between asparagine and aspartic acid (or glutamine and glutamic acid) is when one of the terminal atoms in these residues interacts with a mainchain carbonyl atom or ...
... cannot be located in protein electron-density maps at 1.8 Å resolution, the only situation when it is possible to clearly distinguish between asparagine and aspartic acid (or glutamine and glutamic acid) is when one of the terminal atoms in these residues interacts with a mainchain carbonyl atom or ...
Enzymes responsible for chlorate reduction by Pseudomonas sp
... PDA is unable to respire using perchlorate [7]. The characteristics of the chlorate reductase obtained from strain PDA were substantially different from those from strain KJ. Chlorate reductase activity of strain PDA was found to be associated with the precipitate from the 55–67% saturated ammonium s ...
... PDA is unable to respire using perchlorate [7]. The characteristics of the chlorate reductase obtained from strain PDA were substantially different from those from strain KJ. Chlorate reductase activity of strain PDA was found to be associated with the precipitate from the 55–67% saturated ammonium s ...
Metabolism of acyl‐lipids in Chlamydomonas reinhardtii
... microalgae has been deduced from well characterized pathways of fungi and land plants, but recent advances in molecular and genetic analyses of microalgae have uncovered unique features, pointing out the necessity to study lipid metabolism in microalgae themselves. In the past 10 years, in addition ...
... microalgae has been deduced from well characterized pathways of fungi and land plants, but recent advances in molecular and genetic analyses of microalgae have uncovered unique features, pointing out the necessity to study lipid metabolism in microalgae themselves. In the past 10 years, in addition ...
PPTX - Bonham Chemistry
... • Attenuation is the ability of yeast to metabolize wort sugars • Apparent attenuation is measured by the drop in specific gravity as yeast converts sugar into ethanol and CO2, without correction for alcohol. Real attenuation corrects for alcohol. For normal strength brews, apparent attenuation is a ...
... • Attenuation is the ability of yeast to metabolize wort sugars • Apparent attenuation is measured by the drop in specific gravity as yeast converts sugar into ethanol and CO2, without correction for alcohol. Real attenuation corrects for alcohol. For normal strength brews, apparent attenuation is a ...
THE MULTIFARIOUS AND DYNAMIC REGULATION OF THE LIVING CELL Karen van Eunen
... in ever increasing complexity. The observed poor correlations between mRNA and protein levels [118, 121] suggest that translation and/or protein degradation play a quantitatively important role in the regulation of gene expression. Consequently, quantitative studies of gene expression should not onl ...
... in ever increasing complexity. The observed poor correlations between mRNA and protein levels [118, 121] suggest that translation and/or protein degradation play a quantitatively important role in the regulation of gene expression. Consequently, quantitative studies of gene expression should not onl ...
Of earwax and migration
... machines. For this purpose, pigments were bound with various ingredients such as honey, egg-tempera, gum-arabic and…earwax. As mentioned, cerumen is secreted by cerumen glands in the inner ear by way of biological pumps: the multiple drug resistance proteins. These are organic anion transporters whi ...
... machines. For this purpose, pigments were bound with various ingredients such as honey, egg-tempera, gum-arabic and…earwax. As mentioned, cerumen is secreted by cerumen glands in the inner ear by way of biological pumps: the multiple drug resistance proteins. These are organic anion transporters whi ...
VAAM-Jahrestagung 2015 1.–4. März in Marburg/Lahn
... More and more human SNPs are analyzed for their potential association with diseases, risk factors and predispositions. Our LightSNiP assays are preestablished, probe-based tests using a melting curve to detect sequence variations. These assays are developed on the Roche LightCycler® 480 system, but ...
... More and more human SNPs are analyzed for their potential association with diseases, risk factors and predispositions. Our LightSNiP assays are preestablished, probe-based tests using a melting curve to detect sequence variations. These assays are developed on the Roche LightCycler® 480 system, but ...
The different roles of tryptophan transfer RNA in regulating trp
... respective trp operons and their ancestry. Protein synthesis in all organisms is dependent on the availability of all 20 amino acids, which are charged to their respective tRNAs. Amino acids are provided by biosynthesis, transport from the environment and/or degradation of existing proteins. Chargin ...
... respective trp operons and their ancestry. Protein synthesis in all organisms is dependent on the availability of all 20 amino acids, which are charged to their respective tRNAs. Amino acids are provided by biosynthesis, transport from the environment and/or degradation of existing proteins. Chargin ...
Myr 8, A Novel Unconventional Myosin Expressed during Brain
... cells comprises a prominent feature of mammalian brain development. In the developing neocortex, considerable numbers of neurons exit the cell cycle within the ventricular zone and subsequently initiate an active cell movement along a scaffolding of radial glial cell processes to settle in the corti ...
... cells comprises a prominent feature of mammalian brain development. In the developing neocortex, considerable numbers of neurons exit the cell cycle within the ventricular zone and subsequently initiate an active cell movement along a scaffolding of radial glial cell processes to settle in the corti ...
Bacterial Protein Synthesis Inhibitors (Antimicrobials)
... • Tetracyclines also have a strong affinity for metal ions, forming stable chelates with calcium, magnesium and iron ions. prof. aza ...
... • Tetracyclines also have a strong affinity for metal ions, forming stable chelates with calcium, magnesium and iron ions. prof. aza ...
Mechanistic insights into the regulation of metabolic enzymes by
... acetylation is emerging as a major regulatory mechanism in metabolism that is involved in all three levels of controlling metabolic enzymes and is altered frequently in human diseases. Acetylation rivals other common posttranslational modifications in cell regulation not only in the number of substr ...
... acetylation is emerging as a major regulatory mechanism in metabolism that is involved in all three levels of controlling metabolic enzymes and is altered frequently in human diseases. Acetylation rivals other common posttranslational modifications in cell regulation not only in the number of substr ...
Quantitative Analysis of the Kinetics of End
... RecA protein will form mixed ®laments on DNA with the RecA K72R mutant protein that binds but does not hydrolyze ATP or dATP (Shan & Cox, 1996). In most experiments with the mutant, dATP replaces ATP since the mutant protein functions in some RecA assays only in the presence of dATP (Rehrauer & Kowa ...
... RecA protein will form mixed ®laments on DNA with the RecA K72R mutant protein that binds but does not hydrolyze ATP or dATP (Shan & Cox, 1996). In most experiments with the mutant, dATP replaces ATP since the mutant protein functions in some RecA assays only in the presence of dATP (Rehrauer & Kowa ...
Answers to Quiz Questions
... What are the three single enzyme reactions that contribute to energy transfer in anaerobic metabolism? 1. Enzyme = __________________ ...
... What are the three single enzyme reactions that contribute to energy transfer in anaerobic metabolism? 1. Enzyme = __________________ ...
BAG1, a negative regulator of Hsp70 chaperone activity, uncouples
... for DnaK alone, and in the presence of DnaJ and GrpE (Höhfeld et al., 1997). The turnover rate of DnaK is 0.036 per minute (data not shown), which is similar to that observed by other investigators (Karzai and McMacken, 1996). This intrinsic turnover rate of DnaK is much lower than that of Hsp70. T ...
... for DnaK alone, and in the presence of DnaJ and GrpE (Höhfeld et al., 1997). The turnover rate of DnaK is 0.036 per minute (data not shown), which is similar to that observed by other investigators (Karzai and McMacken, 1996). This intrinsic turnover rate of DnaK is much lower than that of Hsp70. T ...
Peer-reviewed Article PDF
... of 437 amino acid residues are organized into two main subunits, each consisting of 20 alpha-helices. These alpha helices compose approximately 75% of citrate synthase’s tertiary structure. Between these two subunits, a single cleft exists containing the active site. Two binding sites can be found t ...
... of 437 amino acid residues are organized into two main subunits, each consisting of 20 alpha-helices. These alpha helices compose approximately 75% of citrate synthase’s tertiary structure. Between these two subunits, a single cleft exists containing the active site. Two binding sites can be found t ...
Japanese Journal of Ophthalmology Vol.43 No.4
... CAPE, which is an active component of propolis extract, was found to inhibit 5-lipoxygenase in micromolar concentrations, and to block the production of reactive oxygen species in human neutrophils and the xanthine/xanthine oxidase system. It was also believed to contribute to the anti-inflammatory ...
... CAPE, which is an active component of propolis extract, was found to inhibit 5-lipoxygenase in micromolar concentrations, and to block the production of reactive oxygen species in human neutrophils and the xanthine/xanthine oxidase system. It was also believed to contribute to the anti-inflammatory ...
Purification and Some: Characteristics of a Monomeric Racemase
... of other alanine racemases (2, 3, 9, 17) were unsuccessful. This was probably due to the very little amount of purified enzyme obtained, which was only 0.026mg (Table 1). Nevertheless, PLP requirement must be confirmed by further studies. As demonstrated in this study, the enz:lme from T. thermophil ...
... of other alanine racemases (2, 3, 9, 17) were unsuccessful. This was probably due to the very little amount of purified enzyme obtained, which was only 0.026mg (Table 1). Nevertheless, PLP requirement must be confirmed by further studies. As demonstrated in this study, the enz:lme from T. thermophil ...
Investigating The Metal Binding Sites In Znta, A Zinc Transporting
... N1-ZntA confers a Competitive Advantage to ZntA. . . . . . . . . . . . . . . 110 ...
... N1-ZntA confers a Competitive Advantage to ZntA. . . . . . . . . . . . . . . 110 ...
Pathways to abscisic acid-regulated gene expression
... ‘ reverse genetics ’ (starting from unknown ABA-inducible sequences and working backwards) and biochemical studies. What emerges is a cell-biological model of overlapping tissue-specific stress (e.g. drought, salt and cold) and developmental (e.g. sugars and other hormones) response pathways that in ...
... ‘ reverse genetics ’ (starting from unknown ABA-inducible sequences and working backwards) and biochemical studies. What emerges is a cell-biological model of overlapping tissue-specific stress (e.g. drought, salt and cold) and developmental (e.g. sugars and other hormones) response pathways that in ...
Document
... A two-component system bacteria common / well-characterized key mechanism protein phosphorylation ...
... A two-component system bacteria common / well-characterized key mechanism protein phosphorylation ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.