Hormone sensitive lipase: structure, function and
... of triacylglycerol (TAG) to diacylglycerol (DAG) and free fatty acids (FFA). In addition, it has hydrolyzing activity against cholesterol esters. In this sight it is not remarkable that HSL is not only found in adipose tissue, but also in tissues that store cholesterol esters. In these tissues HSL p ...
... of triacylglycerol (TAG) to diacylglycerol (DAG) and free fatty acids (FFA). In addition, it has hydrolyzing activity against cholesterol esters. In this sight it is not remarkable that HSL is not only found in adipose tissue, but also in tissues that store cholesterol esters. In these tissues HSL p ...
Specific features of glycogen metabolism in the liver
... While most extracellular signals control glycogen metabolism via transmembrane signalling pathways, glucose does so by directly binding to phosphorylase a [26]. Since the glucose concentration in the blood and in hepatocytes is the same, phosphorylase acts as a ‘ sensor ’ of the blood glucose level. ...
... While most extracellular signals control glycogen metabolism via transmembrane signalling pathways, glucose does so by directly binding to phosphorylase a [26]. Since the glucose concentration in the blood and in hepatocytes is the same, phosphorylase acts as a ‘ sensor ’ of the blood glucose level. ...
Localization and nucleotide specificity of Blastocystis succinyl‐CoA
... in the absence of a classic mitochondrial electron transport chain, it is likely to be one of the main ATP producing enzymes in this parasite. Here we present the genes encoding both alpha and beta SCS subunits, their phylogenetic analysis, the cellular localization of SCS and a biochemical characte ...
... in the absence of a classic mitochondrial electron transport chain, it is likely to be one of the main ATP producing enzymes in this parasite. Here we present the genes encoding both alpha and beta SCS subunits, their phylogenetic analysis, the cellular localization of SCS and a biochemical characte ...
New insight into the photoheterotrophic growth of the
... production of a compound identified as citramalate (Hoare, 1963) and on the presence of enzymatic activity allowing the production of glyoxylate and propionyl-CoA from C5 compounds (Osumi & Katsuki, 1977). Berg and Ivanovsky’s group also reported the detection of enzymatic activity related to this p ...
... production of a compound identified as citramalate (Hoare, 1963) and on the presence of enzymatic activity allowing the production of glyoxylate and propionyl-CoA from C5 compounds (Osumi & Katsuki, 1977). Berg and Ivanovsky’s group also reported the detection of enzymatic activity related to this p ...
Linear ubiquitin chains: enzymes, mechanisms and biology
... The HOIP subunit of LUBAC contains all the catalytic machinery required to synthesize linear ubiquitin chains with high specificity [2,3]. This activity is located in its C-terminal portion within the RBR domain plus a C-terminal extension referred to as the linear ubiquitin chain-determining domain ...
... The HOIP subunit of LUBAC contains all the catalytic machinery required to synthesize linear ubiquitin chains with high specificity [2,3]. This activity is located in its C-terminal portion within the RBR domain plus a C-terminal extension referred to as the linear ubiquitin chain-determining domain ...
The yeast Saccharomyces cerevisiae– the main
... microbial detection medium such as NBBs (Back, 2005). Modern technology supported the developments of rapid microbiological methods (Russell & Stewart, 2003) such as real-time PCR (Kiehne et al., 2005), multiplex PCR (Asano et al., 2008; Haakensen et al., 2008) and ATP bioluminescence (Simpson & Ham ...
... microbial detection medium such as NBBs (Back, 2005). Modern technology supported the developments of rapid microbiological methods (Russell & Stewart, 2003) such as real-time PCR (Kiehne et al., 2005), multiplex PCR (Asano et al., 2008; Haakensen et al., 2008) and ATP bioluminescence (Simpson & Ham ...
pHAb Amine and Thiol Reactive Dyes
... the antibody after the cysteine disulfide bonds in the hinge region of the antibody are reduced to thiols using a reducing agent, such as DTT or TCEP (Figure 1, Panel B). A key feature of pHAb Dyes is that they have two sulfonate groups per dye, which improve solubility in water and reduce the aggreg ...
... the antibody after the cysteine disulfide bonds in the hinge region of the antibody are reduced to thiols using a reducing agent, such as DTT or TCEP (Figure 1, Panel B). A key feature of pHAb Dyes is that they have two sulfonate groups per dye, which improve solubility in water and reduce the aggreg ...
Organization and Integration of Large-scale Datasets for
... Mycoplasma pneumoniae, one of the smallest known self-replicating organisms, is a promising model organism in systems biology when aiming to assess understanding of an entire living cell. One of the key steps towards this goal is the design of mathematical models that describe the cellular processes ...
... Mycoplasma pneumoniae, one of the smallest known self-replicating organisms, is a promising model organism in systems biology when aiming to assess understanding of an entire living cell. One of the key steps towards this goal is the design of mathematical models that describe the cellular processes ...
Organization and Integration of Large
... Mycoplasma pneumoniae, one of the smallest known self-replicating organisms, is a promising model organism in systems biology when aiming to assess understanding of an entire living cell. One of the key steps towards this goal is the design of mathematical models that describe the cellular processes ...
... Mycoplasma pneumoniae, one of the smallest known self-replicating organisms, is a promising model organism in systems biology when aiming to assess understanding of an entire living cell. One of the key steps towards this goal is the design of mathematical models that describe the cellular processes ...
Affinity Precipitation of a Monoclonal Antibody ELP-Z Stimuli Responsive Biopolymer
... in relatively low titers making it less amenable for large scale mAb production and purification. Kim et al. (2005) have employed an ELP-Protein A fusion for the purification of mAbs via affinity precipitation. In this approach, the mAb is first selectively captured by binding to the ELP-Protein A fusio ...
... in relatively low titers making it less amenable for large scale mAb production and purification. Kim et al. (2005) have employed an ELP-Protein A fusion for the purification of mAbs via affinity precipitation. In this approach, the mAb is first selectively captured by binding to the ELP-Protein A fusio ...
PDF - Beilstein
... occupied by RiPPs means that they possess a wide variety of biological activities, and the class includes antibiotics, co-factors, signalling molecules, anticancer and anti-HIV compounds, and toxins. A considerable amount of RiPP chemical diversity is generated from cyclisation reactions, and the cu ...
... occupied by RiPPs means that they possess a wide variety of biological activities, and the class includes antibiotics, co-factors, signalling molecules, anticancer and anti-HIV compounds, and toxins. A considerable amount of RiPP chemical diversity is generated from cyclisation reactions, and the cu ...
Lipoprotein structure
... and non-denaturing polyacrylamide gel-electrophoresis in the second dimension) have yielded c~-migrating, pref3-migrating, and y-migrating HDL subclasses of various sizes and compositions. The prel3-migrating subclasses are present in low concentrations in plasma (in contrast to the abundant c~ spec ...
... and non-denaturing polyacrylamide gel-electrophoresis in the second dimension) have yielded c~-migrating, pref3-migrating, and y-migrating HDL subclasses of various sizes and compositions. The prel3-migrating subclasses are present in low concentrations in plasma (in contrast to the abundant c~ spec ...
Glucosamine-Induced Insulin Resistance in Primary Rat
... may play a role in induction of insulin resistance and thus needs to be evaluated in different cell types. Therefore, we set out to test whether or not insulin resistance could be established in primary rat hepatocytes. To accomplish this we used a HBP precursor, glucosamine and first assessed wheth ...
... may play a role in induction of insulin resistance and thus needs to be evaluated in different cell types. Therefore, we set out to test whether or not insulin resistance could be established in primary rat hepatocytes. To accomplish this we used a HBP precursor, glucosamine and first assessed wheth ...
Unusual ADP-forming acetyl-coenzyme A synthetases from the
... H. marismortui (DSM 3752) (Oren et al. 1990) was grown aerobically in a 8-l fermentor (FairmenTec, Germany) on a medium containing glucose as carbon and energy source (for detailed description of the media, see Bräsen and Schönheit 2001). Cell extracts were prepared from 25 g (wet weight) of froze ...
... H. marismortui (DSM 3752) (Oren et al. 1990) was grown aerobically in a 8-l fermentor (FairmenTec, Germany) on a medium containing glucose as carbon and energy source (for detailed description of the media, see Bräsen and Schönheit 2001). Cell extracts were prepared from 25 g (wet weight) of froze ...
Technical Brief
... The WHO treatment protocol has been recently updated. 34 With the endorsement of the Communitybased management of severe acute malnutrition in 2007 by WHO and UNICEF35 kwashiorkor cases in the mild and moderate categories, in the absence of complications - the vast majority of cases - are usually tr ...
... The WHO treatment protocol has been recently updated. 34 With the endorsement of the Communitybased management of severe acute malnutrition in 2007 by WHO and UNICEF35 kwashiorkor cases in the mild and moderate categories, in the absence of complications - the vast majority of cases - are usually tr ...
Temperature-dependent effects of cadmium and purine nucleotides
... mitochondrial bioenergetics of eastern oysters Crassostrea virginica, resulting in reduced mitochondrial efficiency, lower rates of ATP synthesis and potential energy deficit (Sokolova, 2004; Cherkasov et al., 2006a,b). Cd-induced discrepancy between energy demand and energy supply is especially str ...
... mitochondrial bioenergetics of eastern oysters Crassostrea virginica, resulting in reduced mitochondrial efficiency, lower rates of ATP synthesis and potential energy deficit (Sokolova, 2004; Cherkasov et al., 2006a,b). Cd-induced discrepancy between energy demand and energy supply is especially str ...
animal nutrition - e
... Adams University College were invited to join the team. These new authors contribute a broad knowledge of animal nutrition and will ensure that the book can go to further editions. In this edition we have attempted to address comments and suggestions made by reviewers in order to improve the book. T ...
... Adams University College were invited to join the team. These new authors contribute a broad knowledge of animal nutrition and will ensure that the book can go to further editions. In this edition we have attempted to address comments and suggestions made by reviewers in order to improve the book. T ...
Calreticulin, a multi-process calcium
... folding and assembly, while calreticulin and calnexin interact with nascent glycoproteins via polypeptide and lectin binding [2]. PDI and ERp57, both thiol oxidoreductase folding factors, utilize the oxidizing environment of the ER to generate disulfide linkages, with the formation of intra- and int ...
... folding and assembly, while calreticulin and calnexin interact with nascent glycoproteins via polypeptide and lectin binding [2]. PDI and ERp57, both thiol oxidoreductase folding factors, utilize the oxidizing environment of the ER to generate disulfide linkages, with the formation of intra- and int ...
Abstracts of the Oral and Poster Contributions
... While scattering techniques are widely used to elucidate the multi-scale structure of soft matter, pathways of their self-assembly process are only beginning to be explored [1]. Quantitative structural studies of the selfassembly process in the nanoscale and millisecond time range are now feasible t ...
... While scattering techniques are widely used to elucidate the multi-scale structure of soft matter, pathways of their self-assembly process are only beginning to be explored [1]. Quantitative structural studies of the selfassembly process in the nanoscale and millisecond time range are now feasible t ...
{PDOC00000} {BEGIN
... dehydrogenase (complex I). - Rhizobium nodulation protein nodF, which probably acts as an ACP in the synthesis of the nodulation Nod factor fatty acyl chain. The sequence around the phosphopantetheine attachment site is conserved in all these proteins and can be used as a signature pattern. -Consens ...
... dehydrogenase (complex I). - Rhizobium nodulation protein nodF, which probably acts as an ACP in the synthesis of the nodulation Nod factor fatty acyl chain. The sequence around the phosphopantetheine attachment site is conserved in all these proteins and can be used as a signature pattern. -Consens ...
The Bacterial Toxin RelE Displays Codon
... of protein synthesis by RelE in vivo. We have found that although RelE does not degrade free RNA, it cleaves mRNA in the ribosomal A site with high codon specificity. Among stop codons UAG is cleaved with fast, UAA intermediate and UGA slow rate, while UCG and CAG are cleaved most rapidly among sens ...
... of protein synthesis by RelE in vivo. We have found that although RelE does not degrade free RNA, it cleaves mRNA in the ribosomal A site with high codon specificity. Among stop codons UAG is cleaved with fast, UAA intermediate and UGA slow rate, while UCG and CAG are cleaved most rapidly among sens ...
Mammalian CSAD and GADL1 have distinct biochemical properties
... mixture of 100 ml containing varying amounts of purified recombinant proteins and amino-acids was prepared in 60 mM potassium phosphate (pH 7.4) containing 5 mM DTT, 50 mM sucrose and 0.5 or 100 mM PLP. Adding 1 mM of the amino acid substrate started the reaction. Samples of 30 ml were taken at 15e60 ...
... mixture of 100 ml containing varying amounts of purified recombinant proteins and amino-acids was prepared in 60 mM potassium phosphate (pH 7.4) containing 5 mM DTT, 50 mM sucrose and 0.5 or 100 mM PLP. Adding 1 mM of the amino acid substrate started the reaction. Samples of 30 ml were taken at 15e60 ...
Liquid Mixtures Involving Hydrogenated and Fluorinated Alcohols
... the excess volumes. This uncovered that, contrarily to what is usually assumed, the volume increase found in these systems cannot be entirely related to weak dispersive interactions between the hydrogenated and fluorinated segments and should be connected to the repulsive part of the intermolecular p ...
... the excess volumes. This uncovered that, contrarily to what is usually assumed, the volume increase found in these systems cannot be entirely related to weak dispersive interactions between the hydrogenated and fluorinated segments and should be connected to the repulsive part of the intermolecular p ...
Novel control of lactate dehydrogenase from the freeze
... Our particular interest in the present study was the potential role of reversible posttranslational modification (PTM) of LDH as a mechanism influencing enzyme function/properties in response to the stresses of freezing and dehydration. PTMs can have strong effects on enzymes including altering acti ...
... Our particular interest in the present study was the potential role of reversible posttranslational modification (PTM) of LDH as a mechanism influencing enzyme function/properties in response to the stresses of freezing and dehydration. PTMs can have strong effects on enzymes including altering acti ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.