Gel electrophoresis - Caltech Particle Theory
... relative to their size or, for cyclic fragments, their radius of gyration. Single-stranded DNA or RNA tend to fold up into molecules with complex shapes and migrate through the gel in a complicated manner based on their tertiary structure. Therefore, agents that disrupt the hydrogen bonds, such as s ...
... relative to their size or, for cyclic fragments, their radius of gyration. Single-stranded DNA or RNA tend to fold up into molecules with complex shapes and migrate through the gel in a complicated manner based on their tertiary structure. Therefore, agents that disrupt the hydrogen bonds, such as s ...
Enzymes - Solon City Schools
... Characteristics of Enzymes 1. Proteins 2. Catalysts a. Speed up chemical reactions without being used up ...
... Characteristics of Enzymes 1. Proteins 2. Catalysts a. Speed up chemical reactions without being used up ...
Fleet Feet Sports Stamford
... healthy choices to ensure we are getting the nutrients for our active bodies. View your daily intake as a “to do” list with your most important items on the top. Whole grains should be on the top of the list (meaning you eat the most of those per day), next fruits and vegetables, followed by lean pr ...
... healthy choices to ensure we are getting the nutrients for our active bodies. View your daily intake as a “to do” list with your most important items on the top. Whole grains should be on the top of the list (meaning you eat the most of those per day), next fruits and vegetables, followed by lean pr ...
Expression of gene encoding immunodominant merozoite surface
... The duplicate wells of ELISA plate were coated separately with Tams1 protein at a concentration of 1,2 or 5 µg per mL of carbonate-bicarbonate buffer (pH 9.6) and titrated with two positive and two negative bovine sera diluted 1:50, 1:100 or 1:200 with PBS (pH 7.2) in standard ELISA format10. The co ...
... The duplicate wells of ELISA plate were coated separately with Tams1 protein at a concentration of 1,2 or 5 µg per mL of carbonate-bicarbonate buffer (pH 9.6) and titrated with two positive and two negative bovine sera diluted 1:50, 1:100 or 1:200 with PBS (pH 7.2) in standard ELISA format10. The co ...
Plant nuclear proteomics inside the cell maestro
... The eukaryotic nucleus is highly dynamic and complex, containing several subcompartments, several types of DNA and RNA, and a wide range of proteins. Interactions between these components within the nucleus form part of a complex regulatory system that is only partially understood. Rapid improvement ...
... The eukaryotic nucleus is highly dynamic and complex, containing several subcompartments, several types of DNA and RNA, and a wide range of proteins. Interactions between these components within the nucleus form part of a complex regulatory system that is only partially understood. Rapid improvement ...
α-Hemolysin pore formation into a supported
... the concurrent cell-free expression and insertion of membrane proteins into phospholipid bilayers. The poreforming protein α-hemolysin, fused to the reporter protein eGFP, was used as a model of membrane protein. Cell-free expression of the toxin in solution and inside large synthetic phospholipid v ...
... the concurrent cell-free expression and insertion of membrane proteins into phospholipid bilayers. The poreforming protein α-hemolysin, fused to the reporter protein eGFP, was used as a model of membrane protein. Cell-free expression of the toxin in solution and inside large synthetic phospholipid v ...
Structure of biological networks
... properties of molecular interaction networks, as well as at local architectural features in terms of characteristic network motifs. ...
... properties of molecular interaction networks, as well as at local architectural features in terms of characteristic network motifs. ...
2. How we study biology • The scientific method requires controls
... Reproduction is the method whereby the survival of the species is assured and at the chemical level this requires the transfer of “information” either as DNA, RNA or some other form of molecule to make protein. All species have a programmed time of cessation of activity (death) which is ultimately d ...
... Reproduction is the method whereby the survival of the species is assured and at the chemical level this requires the transfer of “information” either as DNA, RNA or some other form of molecule to make protein. All species have a programmed time of cessation of activity (death) which is ultimately d ...
Materials and Methods
... Analyses of sequence parameters influencing suitability for NMR structure determination. Nearly 1,000 protein constructs less than 200 amino acids in length that were expressed and soluble at a sufficient level were screened for their suitability for NMR solution-structure determination by the NESG. ...
... Analyses of sequence parameters influencing suitability for NMR structure determination. Nearly 1,000 protein constructs less than 200 amino acids in length that were expressed and soluble at a sufficient level were screened for their suitability for NMR solution-structure determination by the NESG. ...
Presentation
... CLRA_RAT CALCITONIN RECEPTOR A PRECURSOR (CT-R-A) CLRB_RAT CALCITONIN RECEPTOR B PRECURSOR (CT-R-B) DIHR_MANSE DIURETIC HORMONE RECEPTOR (DH-R). CALR_PIG CALCITONIN RECEPTOR PRECURSOR (CT-R). GLR_RAT GLUCAGON RECEPTOR PRECURSOR (GL-R). ...
... CLRA_RAT CALCITONIN RECEPTOR A PRECURSOR (CT-R-A) CLRB_RAT CALCITONIN RECEPTOR B PRECURSOR (CT-R-B) DIHR_MANSE DIURETIC HORMONE RECEPTOR (DH-R). CALR_PIG CALCITONIN RECEPTOR PRECURSOR (CT-R). GLR_RAT GLUCAGON RECEPTOR PRECURSOR (GL-R). ...
Gene regulation in physiological stress
... [10]. Similar methods using an IRES or other novel method of translation initiation may be involved in the selective up-regulation of the stress-responsive genes that support animal adaptation to other challenges (e.g., high or low temperature, freezing, dehydration, heavy metals, etc.). Other contr ...
... [10]. Similar methods using an IRES or other novel method of translation initiation may be involved in the selective up-regulation of the stress-responsive genes that support animal adaptation to other challenges (e.g., high or low temperature, freezing, dehydration, heavy metals, etc.). Other contr ...
Orthologs, paralogs and homology inference Where are we now?
... families, from all RefGenome species, plus 35 other “phylogenetically informative” species • Tree Curation Tool has been updated by Paul’s and Suzi’s groups in collaboration – Retrieves and displays tree, and UniProt information for each sequence – Displays OrthoMCL clustering results-- scalable to ...
... families, from all RefGenome species, plus 35 other “phylogenetically informative” species • Tree Curation Tool has been updated by Paul’s and Suzi’s groups in collaboration – Retrieves and displays tree, and UniProt information for each sequence – Displays OrthoMCL clustering results-- scalable to ...
Ashley, CT, Wilkinson, KD, Reines, D and Warren, ST: FMR1 protein: Conserved RNP family domains and selective RNA binding. Science 262:563-566 (1993).
... parathyroid honnone precursor protein, which does not associate with RNA (20). Only the 69-kD protein, corresponding to the mass of FMRP, was captured from the FMR I in vitro translation mix by the biotinylated RNA. Hydroxylamine cleavage of this 69-kD protein, released from the biotinylated RNA, re ...
... parathyroid honnone precursor protein, which does not associate with RNA (20). Only the 69-kD protein, corresponding to the mass of FMRP, was captured from the FMR I in vitro translation mix by the biotinylated RNA. Hydroxylamine cleavage of this 69-kD protein, released from the biotinylated RNA, re ...
the extracellular matrix
... cross-link cell-surface adhesion receptors and other ECM components The extracellular matrix of epithelial tissues • All epithelial cells in a sheet are connected to one another and the extracellular matrix by specialized cell junctions consisting of dense clusters of CAMs • Anchoring junctions and ...
... cross-link cell-surface adhesion receptors and other ECM components The extracellular matrix of epithelial tissues • All epithelial cells in a sheet are connected to one another and the extracellular matrix by specialized cell junctions consisting of dense clusters of CAMs • Anchoring junctions and ...
Chapter 5 Macromolecules
... • Each cell has thousands of different macromolecules • Macromolecules vary among cells of an organism, vary more within a species, and vary even more between species • An immense variety of polymers can be built from a small set of monomers HO ...
... • Each cell has thousands of different macromolecules • Macromolecules vary among cells of an organism, vary more within a species, and vary even more between species • An immense variety of polymers can be built from a small set of monomers HO ...
Document
... • additional membrane is needed for cell expansion; synthesize new membranes only by expansion of existing membranes • the basic structural and physical properties of membranes are determined by their lipid components—principally phospholipids, sphingolipids, and sterols such as cholesterol • early ...
... • additional membrane is needed for cell expansion; synthesize new membranes only by expansion of existing membranes • the basic structural and physical properties of membranes are determined by their lipid components—principally phospholipids, sphingolipids, and sterols such as cholesterol • early ...
The cloning and overexpression of E coli acyl carrier protein (ACP)
... which use type I1 dissociable fatty acid synthetases, acyl chains are esterified to ACP through the 4'-phosphopantetheineprosthetic group, and ACP acts as a carrier during fatty acid synthesis. ACPs from bacteria and plants are very similar. All are small acidic proteins with conserved regions parti ...
... which use type I1 dissociable fatty acid synthetases, acyl chains are esterified to ACP through the 4'-phosphopantetheineprosthetic group, and ACP acts as a carrier during fatty acid synthesis. ACPs from bacteria and plants are very similar. All are small acidic proteins with conserved regions parti ...
Organic molecules
... Plants and animals typically contain a lot of glucose. Most soft drinks such as Coke, Pepsi and Sprite contain fructose instead of glucose. Why is this? _____________________________________________________________ _____________________________________________________________ ...
... Plants and animals typically contain a lot of glucose. Most soft drinks such as Coke, Pepsi and Sprite contain fructose instead of glucose. Why is this? _____________________________________________________________ _____________________________________________________________ ...
ELECTROPHORESIS
... oppositely charged electrodes on the basis of their molecular size. 5. Smaller DNA molecules will travel faster as compared to larger ones. Commonly used buffers are tris acetate, tris borate or tris phosphate at a concentration of 50mM over a pH range of 7.5-8.0. ...
... oppositely charged electrodes on the basis of their molecular size. 5. Smaller DNA molecules will travel faster as compared to larger ones. Commonly used buffers are tris acetate, tris borate or tris phosphate at a concentration of 50mM over a pH range of 7.5-8.0. ...
Protein Modeling
... energy of the system also. 1,4 VDW: is the energy that relates to the Vander-Waal’s force. 1,4 VDW energy is experienced between 2 atoms that are apart from each other. 1,4 VDW energy doesn’t really affect the total energy much since its change is not significant compared to others; 8.711 kcal/mol i ...
... energy of the system also. 1,4 VDW: is the energy that relates to the Vander-Waal’s force. 1,4 VDW energy is experienced between 2 atoms that are apart from each other. 1,4 VDW energy doesn’t really affect the total energy much since its change is not significant compared to others; 8.711 kcal/mol i ...
Part I - Punjabi University
... 10. Amino acid metabolism: biosynthetic families of amino acids, ammonia ion assimilation into amino acid by Glu and Gln, regulation of amino acid synthesis, Degradation of amino acids: oxidative deamination of glutamate, carbon atom degradation, amino acid as major metabolic intermediates, C3, C4 a ...
... 10. Amino acid metabolism: biosynthetic families of amino acids, ammonia ion assimilation into amino acid by Glu and Gln, regulation of amino acid synthesis, Degradation of amino acids: oxidative deamination of glutamate, carbon atom degradation, amino acid as major metabolic intermediates, C3, C4 a ...
Derivation and testing of pair potentials for protein folding. When is
... contact probability as being proportional to the product of the mole fractions of the pair of residues would appear to be rather severe. To investigate the validity of this approximation, we introduce two new reference states in which no specific pair interactions between amino acids areallowed, but ...
... contact probability as being proportional to the product of the mole fractions of the pair of residues would appear to be rather severe. To investigate the validity of this approximation, we introduce two new reference states in which no specific pair interactions between amino acids areallowed, but ...
Chapter 4.32
... traverses the endoplasmic reticulum (ER) and Golgi compartments. In N-glycosylation, a preformed oligosaccharide moiety is transferred en bloc from a long-chain isoprenoid lipid (dolichol) onto the specific N-glycosylation site via an N-glycosidic linkage to the asparagine (Asn) residue of a nascent ...
... traverses the endoplasmic reticulum (ER) and Golgi compartments. In N-glycosylation, a preformed oligosaccharide moiety is transferred en bloc from a long-chain isoprenoid lipid (dolichol) onto the specific N-glycosylation site via an N-glycosidic linkage to the asparagine (Asn) residue of a nascent ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.