Plant Physiology
... 1994),a lectin that binds only oligosaccharides (Sharon and Lis, 1990), and are nearly identical with residues in comparable positions of other legume lectins. However, only the first three of these six amino acid residues are identical with those of MtLECl, which is believed to be a Glc/Manbinding ...
... 1994),a lectin that binds only oligosaccharides (Sharon and Lis, 1990), and are nearly identical with residues in comparable positions of other legume lectins. However, only the first three of these six amino acid residues are identical with those of MtLECl, which is believed to be a Glc/Manbinding ...
Controlling subcellular delivery to optimize
... protonated in the acidic environment of the endosomes. The ‘proton sponge effect’ causes osmotic swelling and rupture of the endosomes [62] . The main drawback of PEI is its toxicity [63,64] , but many modifications of PEI have been made in an attempt to circumvent this [65–69] . Many other techniqu ...
... protonated in the acidic environment of the endosomes. The ‘proton sponge effect’ causes osmotic swelling and rupture of the endosomes [62] . The main drawback of PEI is its toxicity [63,64] , but many modifications of PEI have been made in an attempt to circumvent this [65–69] . Many other techniqu ...
Immunoprecipitation protocol
... the serum Ig is being removed effectively. If serum has not been sufficiently removed, bands will be present at 50 and 25 kDa for heavy and light chains; its presence may contribute to a weak immunoprecipitation. Consider either decreasing the amount of serum or increasing the amount of beads incuba ...
... the serum Ig is being removed effectively. If serum has not been sufficiently removed, bands will be present at 50 and 25 kDa for heavy and light chains; its presence may contribute to a weak immunoprecipitation. Consider either decreasing the amount of serum or increasing the amount of beads incuba ...
Modification-specific proteomics: Strategies for characterization of
... for identifying candidate substrate proteins for certain types of PTM, such as tyrosine phosphorylation. However, the small size of the structural motifs of other common PTMs (for example, protein methylation and acetylation) makes it difficult to generate pan-specific antibodies, which recognize PT ...
... for identifying candidate substrate proteins for certain types of PTM, such as tyrosine phosphorylation. However, the small size of the structural motifs of other common PTMs (for example, protein methylation and acetylation) makes it difficult to generate pan-specific antibodies, which recognize PT ...
The significance of biochemical and molecular sample integrity in
... In a first experiment 16 mice were randomly divided into four groups (Table 1). The first group was sacrificed by focused microwave irradiation (Muromachi Kikai, Tokyo, Japan), 1.4 s at 4.5–5 kW (control group). Three groups of animals were sacrificed by cervical dislocation and kept in room tempera ...
... In a first experiment 16 mice were randomly divided into four groups (Table 1). The first group was sacrificed by focused microwave irradiation (Muromachi Kikai, Tokyo, Japan), 1.4 s at 4.5–5 kW (control group). Three groups of animals were sacrificed by cervical dislocation and kept in room tempera ...
PDF version of paper
... cells through bloodstream, actin that provides stability to cells are some examples of proteins. Proteins are encoded by specific genes. Information in DNA (in the form of 4 letters: A or T or G or C) are translated into amino acids (20 different letters) during protein synthesis. Therefore, analyzi ...
... cells through bloodstream, actin that provides stability to cells are some examples of proteins. Proteins are encoded by specific genes. Information in DNA (in the form of 4 letters: A or T or G or C) are translated into amino acids (20 different letters) during protein synthesis. Therefore, analyzi ...
144803525 - BORA
... established that phosphorylation at Ser31 and Ser40 (THpSer31 and THpSer40, respectively) leads to an increase of TH activity. However, phosphorylation of Ser19 does not directly increase TH activity but induces its binding to the 14-3-3 protein, leading to the enhancement of TH activity through a m ...
... established that phosphorylation at Ser31 and Ser40 (THpSer31 and THpSer40, respectively) leads to an increase of TH activity. However, phosphorylation of Ser19 does not directly increase TH activity but induces its binding to the 14-3-3 protein, leading to the enhancement of TH activity through a m ...
NUCLEAR PROTEINS II. Similarity of Nonhistone Proteins in
... a- and /3-Tropomyosin (16, 17) consistently comigrated with the bands H10 and H5, respectively. Preliminary studies (see below) suggest that these are H n R N P proteins rather than a- and fltropomyosin. Bands C10, D1, and D4, which occur in both the washes and are prominent on washed nuclei, have b ...
... a- and /3-Tropomyosin (16, 17) consistently comigrated with the bands H10 and H5, respectively. Preliminary studies (see below) suggest that these are H n R N P proteins rather than a- and fltropomyosin. Bands C10, D1, and D4, which occur in both the washes and are prominent on washed nuclei, have b ...
Choosing an antibody
... The species the primary antibody is raised in should be different from the species of your sample. This is to avoid cross-reactivity of the secondary anti-immunoglobulin antibody with endogenous immunoglobulins in the sample. For instance, if you are studying a mouse protein, choose a primary antibo ...
... The species the primary antibody is raised in should be different from the species of your sample. This is to avoid cross-reactivity of the secondary anti-immunoglobulin antibody with endogenous immunoglobulins in the sample. For instance, if you are studying a mouse protein, choose a primary antibo ...
Amino Acids in Dairy Nutrition – Where Do They Fit?
... The concept underlying amino acid balancing is straightforward and is simply a comparison of amino acid flow to the small intestine compared with amino acid requirements for synthesis of milk and tissue. The amino acid makeup of tissue and milk protein is encoded in the genes present in the cow; the ...
... The concept underlying amino acid balancing is straightforward and is simply a comparison of amino acid flow to the small intestine compared with amino acid requirements for synthesis of milk and tissue. The amino acid makeup of tissue and milk protein is encoded in the genes present in the cow; the ...
Binding of a Growth Hormone- Inducible Nuclear Factor Is Mediated
... are part of the phosphorylationcascadein the response after GH binding to GH receptor in 3T3-F442A preadipocytes (22, 23). This also was noted in Chinesehamster ovary cells transfected with GH receptor (24) and in GH-treated liver nuclear extracts (12). Ultimately, many aspectsof MAP kinase-activate ...
... are part of the phosphorylationcascadein the response after GH binding to GH receptor in 3T3-F442A preadipocytes (22, 23). This also was noted in Chinesehamster ovary cells transfected with GH receptor (24) and in GH-treated liver nuclear extracts (12). Ultimately, many aspectsof MAP kinase-activate ...
MNS Blood Group System variants on Malarial Resistance
... Segment of genetic material from one chromosome is copied onto the other without changes in the donor chromosome. ...
... Segment of genetic material from one chromosome is copied onto the other without changes in the donor chromosome. ...
Supplementary Figures 1 - 5, Methods
... The expression of Globo-H on (A) MCF-7 cells and (B) MDA-MB-157 cells was determined by flow cytometry with mAbVK9. (C) Microvesicles (MVs, 50g) harvested from the supernatants of MDA-MB-157 cells (MB-MVs) were incubated with HUVECs for 4h and examined for surface Globo-H. (D) Serial dilutions of M ...
... The expression of Globo-H on (A) MCF-7 cells and (B) MDA-MB-157 cells was determined by flow cytometry with mAbVK9. (C) Microvesicles (MVs, 50g) harvested from the supernatants of MDA-MB-157 cells (MB-MVs) were incubated with HUVECs for 4h and examined for surface Globo-H. (D) Serial dilutions of M ...
Cancer Prone Disease Section Oculocutaneous Albinism Atlas of Genetics and Cytogenetics
... similar to exons IV and V of TYR gene. It is hypothesized that duplication of TYR exons IV and V regions followed by 11q:11p translocation has given rise to the TYRL segment. Protein Description TYR (monophenol monoxygenase EC 1.14.18.1) encodes a ~80 kDa glycoprotein (Accession No: NP_000363.1) com ...
... similar to exons IV and V of TYR gene. It is hypothesized that duplication of TYR exons IV and V regions followed by 11q:11p translocation has given rise to the TYRL segment. Protein Description TYR (monophenol monoxygenase EC 1.14.18.1) encodes a ~80 kDa glycoprotein (Accession No: NP_000363.1) com ...
sickle cell anemia explained by protein shape, northeast 2012
... already been introduced to major themes in biology, such as the chemistry of life (including atomic structure and types of chemical bonds). They will have just been introduced to the four major types of macromolecules: their building blocks, chemical properties, and important functional groups. The ...
... already been introduced to major themes in biology, such as the chemistry of life (including atomic structure and types of chemical bonds). They will have just been introduced to the four major types of macromolecules: their building blocks, chemical properties, and important functional groups. The ...
ARTÍCULOS
... of global modulators that also includes the YmoA protein from Yersinia enterocolitica. This interaction has been found to be involved in the regulation of the expression of the toxin ␣-hemolysin. In this study, we further characterize the interaction between H-NS and Hha. We show that the presence o ...
... of global modulators that also includes the YmoA protein from Yersinia enterocolitica. This interaction has been found to be involved in the regulation of the expression of the toxin ␣-hemolysin. In this study, we further characterize the interaction between H-NS and Hha. We show that the presence o ...
Pathways of Pyrimidine and Purine Metabolism in E.coli
... Because mammals lack these enzymes, a better understanding of these molecules may make them attractive targets for drug therapy. This study attempted to characterize the active site of the inosine-uridine hydrolase of E. coli, encoded by rihC. Methods: Specific amino acid residues of the rihC gene o ...
... Because mammals lack these enzymes, a better understanding of these molecules may make them attractive targets for drug therapy. This study attempted to characterize the active site of the inosine-uridine hydrolase of E. coli, encoded by rihC. Methods: Specific amino acid residues of the rihC gene o ...
IJEB 48(4) 373-377
... The diet rich in riboflavin increased the induction period of liver tumours by azo-dyes21. Similary, rats fed with both riboflavin and cupric oxyacetate hexahydrate delayed the formation of liver tumour induced by azo-dyes18. The results of the present study also indicate that supplementation of vit ...
... The diet rich in riboflavin increased the induction period of liver tumours by azo-dyes21. Similary, rats fed with both riboflavin and cupric oxyacetate hexahydrate delayed the formation of liver tumour induced by azo-dyes18. The results of the present study also indicate that supplementation of vit ...
Print - Circulation Research
... was maintained for an identical period in the same medium plus the experimental agent. In some experiments, the final media (both control and experimental) contained cycloheximide, 2.5 /xg/ml, to block protein synthesis and reutilization of label. For experiments involving chloroquine, initial stabi ...
... was maintained for an identical period in the same medium plus the experimental agent. In some experiments, the final media (both control and experimental) contained cycloheximide, 2.5 /xg/ml, to block protein synthesis and reutilization of label. For experiments involving chloroquine, initial stabi ...
Comparative analysis of proteins with a mucus
... The putative MUB domain of the protein lp_1229 of Lb. plantarum WCFS1 consists of approximately 100 aa, while the MUB domain of protein Mub of Lb. reuteri is almost 200 residues in length (Kleerebezem et al., 2003; Pretzer et al., 2005; Roos & Jonsson, 2002). This difference in size implies a discre ...
... The putative MUB domain of the protein lp_1229 of Lb. plantarum WCFS1 consists of approximately 100 aa, while the MUB domain of protein Mub of Lb. reuteri is almost 200 residues in length (Kleerebezem et al., 2003; Pretzer et al., 2005; Roos & Jonsson, 2002). This difference in size implies a discre ...
Polypeptide Composition of Envelopes of Spinach Chloroplasts
... branes which were not fully separated from inner envelope membranes. Thirty amino acid residues of the N-terminus of the 15 kDa protein were determined (Fig. 3A). Homology search of the protein against a DNA database revealed that it is homologous to the 16 kDa protein of outer envelopes (OEP16) fro ...
... branes which were not fully separated from inner envelope membranes. Thirty amino acid residues of the N-terminus of the 15 kDa protein were determined (Fig. 3A). Homology search of the protein against a DNA database revealed that it is homologous to the 16 kDa protein of outer envelopes (OEP16) fro ...
Analyzing Effects of Naturally Occurring Missense Mutations
... products; therefore, any conformational change altering the active sites will also affect the biochemical reaction; however, conservative mutations are not expected to perturb protein function by much. Thus, even if the mutation does not occur at the active site, but quite close to it, the characteri ...
... products; therefore, any conformational change altering the active sites will also affect the biochemical reaction; however, conservative mutations are not expected to perturb protein function by much. Thus, even if the mutation does not occur at the active site, but quite close to it, the characteri ...
Paper Title
... First approach was based on the idea of selecting a subset of properties with high orthogonality. To prove this idea, 544 properties from AAIndex were clustered by K-means algorithm [20] into {5, 6, 7, 8, 9, 10} clusters and 20,000 of experiments were proceeded on MMP dataset for each cluster count ...
... First approach was based on the idea of selecting a subset of properties with high orthogonality. To prove this idea, 544 properties from AAIndex were clustered by K-means algorithm [20] into {5, 6, 7, 8, 9, 10} clusters and 20,000 of experiments were proceeded on MMP dataset for each cluster count ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.