Modulating the cobalt redox potential through imidazole hydrogen
... the cofactor is replaced by a protein histidine (“His”) anchoring group (“His-on” conguration). This mode of protein-cofactor binding is shown in Fig. 1 (ref. 10) and encountered, amongst others, in MeCbl-dependent methionine synthase (MetH) as well as AdoCbl dependent methylmalonyl CoA mutase (MCM ...
... the cofactor is replaced by a protein histidine (“His”) anchoring group (“His-on” conguration). This mode of protein-cofactor binding is shown in Fig. 1 (ref. 10) and encountered, amongst others, in MeCbl-dependent methionine synthase (MetH) as well as AdoCbl dependent methylmalonyl CoA mutase (MCM ...
7.06 Cell Biology EXAM #3
... And be sure to put your NAME ON EACH PAGE in case they become separated! There are SEVEN pages including this cover sheet. ...
... And be sure to put your NAME ON EACH PAGE in case they become separated! There are SEVEN pages including this cover sheet. ...
Statistical potential-based amino acid similarity
... By definition, commonly used amino acid similarity matrices (AA matrices) that are derived from a set of homologous proteins, such as the BLOSUM36 or PAM37 series, do not work well for proteins of fold level similarity. Note here that if a better AA matrix for distantly related proteins is developed, ...
... By definition, commonly used amino acid similarity matrices (AA matrices) that are derived from a set of homologous proteins, such as the BLOSUM36 or PAM37 series, do not work well for proteins of fold level similarity. Note here that if a better AA matrix for distantly related proteins is developed, ...
Amino Acids and Proteins Amino Acid Compound
... forces involve the interactions that occur when uncharged non-bonded atoms come very close together but do not induce dipoles. The repulsion is the result of the electron-electron repulsion that occurs as two clouds of electrons begin to overlap. Although van der Waals forces are extremely weak, rel ...
... forces involve the interactions that occur when uncharged non-bonded atoms come very close together but do not induce dipoles. The repulsion is the result of the electron-electron repulsion that occurs as two clouds of electrons begin to overlap. Although van der Waals forces are extremely weak, rel ...
and gender-specific hallmarks
... coverage and results in a poor representation of low-abundant or very hydrophobic proteins as well as those with extreme pI and molecular weight13,14. On the other hand, current high-throughput proteomic techniques enable reliable detection and quantitation of thousands of proteins in complex proteo ...
... coverage and results in a poor representation of low-abundant or very hydrophobic proteins as well as those with extreme pI and molecular weight13,14. On the other hand, current high-throughput proteomic techniques enable reliable detection and quantitation of thousands of proteins in complex proteo ...
Lecture 3 Isoelectric Focusing
... a solution containing the mixture of carrier ampholytes - the carrier ampholytes with the highest pI (and the most positive charge) move toward the cathode. -the carrier ampholytes with the lowest pI (and the most negative charge) move toward the anode -other carrier ampholytes align themselves betw ...
... a solution containing the mixture of carrier ampholytes - the carrier ampholytes with the highest pI (and the most positive charge) move toward the cathode. -the carrier ampholytes with the lowest pI (and the most negative charge) move toward the anode -other carrier ampholytes align themselves betw ...
A Personal Account of Chaperonin History
... these observations were made immediately after Anfinsen’s seminal work (1) that provided the dominating intellectual framework in the 1970s and 1980s in all matters related to protein folding. By then the prevailing dogma was that protein folding was a spontaneous event and suggesting that the foldi ...
... these observations were made immediately after Anfinsen’s seminal work (1) that provided the dominating intellectual framework in the 1970s and 1980s in all matters related to protein folding. By then the prevailing dogma was that protein folding was a spontaneous event and suggesting that the foldi ...
New weapons against inflammation: dual inhibitors of
... glucocorticoids in the lung that exhibit anti-inflammatory activity in experimental models. These are the lipocortins, or annexins (2), and the secretoglobins, whose prototype is uteroglobin (3). These families include proteins with distinct and pleiotropic biological properties. Lipocortins I and V ...
... glucocorticoids in the lung that exhibit anti-inflammatory activity in experimental models. These are the lipocortins, or annexins (2), and the secretoglobins, whose prototype is uteroglobin (3). These families include proteins with distinct and pleiotropic biological properties. Lipocortins I and V ...
Egg Components Dong Ahn Animal Science Department Iowa State University
... Sialic acid derivatives (gangliosides) are involved in brain function Important in protecting infants from various diseases. Sialic acid and sialyloligosaccharides have potential biological functions. Potential to be used in infant formula, health foods and nutritional ...
... Sialic acid derivatives (gangliosides) are involved in brain function Important in protecting infants from various diseases. Sialic acid and sialyloligosaccharides have potential biological functions. Potential to be used in infant formula, health foods and nutritional ...
Bio Exam 4 Study Guide- Question Format Fatty acid Synthesis
... a. Unsaturated FA containing more than one double bond 30. Single bonded chains are catalyzed into double bonded molecules through what process? a. Desaturases catalyze double bond formation by using NADH and O2. 31. Where can desaturases place the double bond? Where can they not? a. Between carbony ...
... a. Unsaturated FA containing more than one double bond 30. Single bonded chains are catalyzed into double bonded molecules through what process? a. Desaturases catalyze double bond formation by using NADH and O2. 31. Where can desaturases place the double bond? Where can they not? a. Between carbony ...
Uncertainty calculation
... Where xdet is the number of moles of protein present in the sample as determined by the calibration curve, yj is the measured peak area ratio at each calibration point, b1 is the calculated best fit gradient, b0 is the calculated intercept, n is the total number of data points used for the calculati ...
... Where xdet is the number of moles of protein present in the sample as determined by the calibration curve, yj is the measured peak area ratio at each calibration point, b1 is the calculated best fit gradient, b0 is the calculated intercept, n is the total number of data points used for the calculati ...
COURSE SYLLABUS CHM 521 Biochemistry I 3(3
... The pentose phosphate pathway Glycogen breakdown and synthesis Regulation of carbohydrate metabolism Hormones and second messengers Oxidation of pyruvate The citric acid cycle Anabolic TCA cycle and glyoxylate cycle Regulation of the citric acid cycle Di- and oligosaccharide metabolism Mitochondria ...
... The pentose phosphate pathway Glycogen breakdown and synthesis Regulation of carbohydrate metabolism Hormones and second messengers Oxidation of pyruvate The citric acid cycle Anabolic TCA cycle and glyoxylate cycle Regulation of the citric acid cycle Di- and oligosaccharide metabolism Mitochondria ...
PBI 6 Features on Teacher`s Map 2-08.qxp
... Splice sites that conform closely to these consensus sequences are very efficient, while other sites that do not match the consensus as well are less efficient. As a result, the level of expression of a given gene can be controlled, in part, by the efficiency of splicing of its premRNA. (See Mutatio ...
... Splice sites that conform closely to these consensus sequences are very efficient, while other sites that do not match the consensus as well are less efficient. As a result, the level of expression of a given gene can be controlled, in part, by the efficiency of splicing of its premRNA. (See Mutatio ...
SUPPLEMENTARY MATERIAL Title: Luteolin attenuate the D
... Thr180/Tyr182-phosphorylated p38MAPK (Phospho-p38 MAPK, Sigma-Aldrich, St. Louis, MO, USA) at a 1:500 dilution at 4°C overnight. Each membrane was thrice rinsed for 15 min and incubated with the secondary horseradish peroxidase-linked antibodies. β-actin was used as an internal control for the cytos ...
... Thr180/Tyr182-phosphorylated p38MAPK (Phospho-p38 MAPK, Sigma-Aldrich, St. Louis, MO, USA) at a 1:500 dilution at 4°C overnight. Each membrane was thrice rinsed for 15 min and incubated with the secondary horseradish peroxidase-linked antibodies. β-actin was used as an internal control for the cytos ...
calculating the structure-based phylogenetic relationship
... This work is dedicated to my mother, father, and brother; they helped when I required it most. ...
... This work is dedicated to my mother, father, and brother; they helped when I required it most. ...
Defining How Botulinum Toxin Binds to the
... Recently, the Stevens Laboratory at The Scripps Research Institute, in collaboration with the Marks laboratory at UCSF and the Chapman and Johnson laboratories at the University of Wisconsin, Madison, completed structural studies on the structures of botulinum toxin in complex with the neuronal cell ...
... Recently, the Stevens Laboratory at The Scripps Research Institute, in collaboration with the Marks laboratory at UCSF and the Chapman and Johnson laboratories at the University of Wisconsin, Madison, completed structural studies on the structures of botulinum toxin in complex with the neuronal cell ...
Characterizing the complexity of enzymes on the basis of their
... toolkit – amino acids and cofactors The catalytic site is the enzyme’s workshop, and it is here that the catalytic reaction occurs. This cleft, which is often buried (sometimes deeply) [30], houses a relatively small number of amino acids that are involved in binding the substrate (and ⁄ or cofactor ...
... toolkit – amino acids and cofactors The catalytic site is the enzyme’s workshop, and it is here that the catalytic reaction occurs. This cleft, which is often buried (sometimes deeply) [30], houses a relatively small number of amino acids that are involved in binding the substrate (and ⁄ or cofactor ...
Monomers are the
... Q: What monomer are complex carbohydrates made out of? A: simple carbohydrates = sugars = monosaccharides Q: What do the names of all carbohydrates end with? A: “- ose” Q: Compare and contrast starch and cellulose. ...
... Q: What monomer are complex carbohydrates made out of? A: simple carbohydrates = sugars = monosaccharides Q: What do the names of all carbohydrates end with? A: “- ose” Q: Compare and contrast starch and cellulose. ...
Slayer of Lactobacillus helveticus ATCC 12046
... Extraction of the S-layer protein with LiCl and S-layer re-formation on growing cells. Exponential-phasecells were harvested and washed once with distilled water. Ten-fifteen mg of the moist pellet were suspended per ml of 5 M-LiCl and kept at 0 "C for 15 min, followed by centrifugation (30000 g, 15 ...
... Extraction of the S-layer protein with LiCl and S-layer re-formation on growing cells. Exponential-phasecells were harvested and washed once with distilled water. Ten-fifteen mg of the moist pellet were suspended per ml of 5 M-LiCl and kept at 0 "C for 15 min, followed by centrifugation (30000 g, 15 ...
Peptide microarrays for detailed, high-throughput
... As a first step toward understanding the behavior of kinase inhibitors in a cellular context, and ultimately in vivo, the effect on the target kinase is usually studied in vitro. Using standard kinase assays, such an approach is laborious and time-consuming, whereas the use of a peptide microarray m ...
... As a first step toward understanding the behavior of kinase inhibitors in a cellular context, and ultimately in vivo, the effect on the target kinase is usually studied in vitro. Using standard kinase assays, such an approach is laborious and time-consuming, whereas the use of a peptide microarray m ...
REGULATORY ENZYMES
... Michaelis-Menten curves), while an allosteric inhibitor (-) will result in more pronounced sigmoidal curves. The sigmoidicity is thought to result from the cooperativity of structural changes between enzyme subunits (again similar to oxygen binding to hemoglobin). NOTE: A true Km cannot be determine ...
... Michaelis-Menten curves), while an allosteric inhibitor (-) will result in more pronounced sigmoidal curves. The sigmoidicity is thought to result from the cooperativity of structural changes between enzyme subunits (again similar to oxygen binding to hemoglobin). NOTE: A true Km cannot be determine ...
Glycosylation and Sorting of Secretory Proteins in the Endoplasmic
... signal actively recruited another normally Sec24p-dependent reporter protein for Sec24p-independent ER exit, when the C-terminal domain was fused to the reporter protein. Since Hsp150 is a soluble protein, the sorting signal is likely to interact with a putative transmembrane adapter protein that ma ...
... signal actively recruited another normally Sec24p-dependent reporter protein for Sec24p-independent ER exit, when the C-terminal domain was fused to the reporter protein. Since Hsp150 is a soluble protein, the sorting signal is likely to interact with a putative transmembrane adapter protein that ma ...
Sequence-Specific Deoxyribonucleic Acid (DNA) Recognition by
... DNA complex largely mimic the general trend observed for other NR-DNA complexes. Figure 3 catalogs the intermolecular hydrogen bonding and electrostatic interactions that are consistently detected in the majority of the structures comprising the NMR ensemble. A Glu-Lys pair consisting of Glu31 and L ...
... DNA complex largely mimic the general trend observed for other NR-DNA complexes. Figure 3 catalogs the intermolecular hydrogen bonding and electrostatic interactions that are consistently detected in the majority of the structures comprising the NMR ensemble. A Glu-Lys pair consisting of Glu31 and L ...
View PDF - Faculty Web Sites at the University of Virginia
... male germ cells, and of mitotic chromosomes in early embryonic cells [6,14]. To the amazement of many workers in the motor protein field, ncd, in contrast to kinesin, was shown to generate force toward the microtubule minus end [ 30**,31**]. The ncd protein also generates torque, causing microtubule ...
... male germ cells, and of mitotic chromosomes in early embryonic cells [6,14]. To the amazement of many workers in the motor protein field, ncd, in contrast to kinesin, was shown to generate force toward the microtubule minus end [ 30**,31**]. The ncd protein also generates torque, causing microtubule ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.