Cell Chemistry
... • High-energy electrons from NADH and FADH2 are transferred through a series of carriers in the membrane • e- carriers organized in ET complexes I, II, III, IV • Low energy electrons from IV carried on O2 +2H+ to form H2O • energy from ETC is used to pump protons to intermembrane space ...
... • High-energy electrons from NADH and FADH2 are transferred through a series of carriers in the membrane • e- carriers organized in ET complexes I, II, III, IV • Low energy electrons from IV carried on O2 +2H+ to form H2O • energy from ETC is used to pump protons to intermembrane space ...
Cellular Communication
... – Bind to membrane receptors on cell surface – Primary effect: turn enzymes on/off Æ D activity of cell. – Secondary effect: enzymes may produce or activate transcription factors Æ turn genes on/off. • Lipophilic signal molecules — most fatty acid class – Bind to intracellular receptors in cytoplasm ...
... – Bind to membrane receptors on cell surface – Primary effect: turn enzymes on/off Æ D activity of cell. – Secondary effect: enzymes may produce or activate transcription factors Æ turn genes on/off. • Lipophilic signal molecules — most fatty acid class – Bind to intracellular receptors in cytoplasm ...
Chapter 26: Biomolecules: Amino Acids Peptides and Proteins
... • In basic solution, the groups are in their base forms, an overall anion • In neutral solution cation and anion forms are present • This pH where the overall charge is 0 is the isoelectric point, pI ...
... • In basic solution, the groups are in their base forms, an overall anion • In neutral solution cation and anion forms are present • This pH where the overall charge is 0 is the isoelectric point, pI ...
Changes to AAFCO Profiles for Dogs and Cats
... Dogs and cats do not need “protein” but rather the amino acids that make up protein Nevertheless, using normal ingredients it is reasonable to state a minimum protein concentration – Specific amino acid minimums are also added to assure protein quality ...
... Dogs and cats do not need “protein” but rather the amino acids that make up protein Nevertheless, using normal ingredients it is reasonable to state a minimum protein concentration – Specific amino acid minimums are also added to assure protein quality ...
Week 10 notes
... – linear arrangement of amino acids in the polypeptide (like beads on a string) – exact sequence of amino acids determines overall protein structure (analogy: different arrangements of letters spell out words with different meanings) – all proteins have primary structure ...
... – linear arrangement of amino acids in the polypeptide (like beads on a string) – exact sequence of amino acids determines overall protein structure (analogy: different arrangements of letters spell out words with different meanings) – all proteins have primary structure ...
PPT CH 18
... • Disulfide bridges between two cysteine residues • Salt bridges between ionic side chains -COO- and -NH3+ • Hydrogen bonds between polar residue side chains • Hydrophobic interactions: two nonpolar groups are attracted by a mutual repulsion of water ...
... • Disulfide bridges between two cysteine residues • Salt bridges between ionic side chains -COO- and -NH3+ • Hydrogen bonds between polar residue side chains • Hydrophobic interactions: two nonpolar groups are attracted by a mutual repulsion of water ...
Gene Section CREB3L2 (cAMP responsive element binding
... CREB3L2 protein contains a consensus B-ZIP domain highly similar to that in the CREB3L1, CREB3L3, CREB3L4, CREB3 and Drosophila Bbf-2 transcription factors with 80, 60, 59, 56 and 71% identity, respectively. It also contains the amino acid sequence RRKKKEY which is exactly conserved among CREB, CREM ...
... CREB3L2 protein contains a consensus B-ZIP domain highly similar to that in the CREB3L1, CREB3L3, CREB3L4, CREB3 and Drosophila Bbf-2 transcription factors with 80, 60, 59, 56 and 71% identity, respectively. It also contains the amino acid sequence RRKKKEY which is exactly conserved among CREB, CREM ...
The Three-Dimensional Structure of the 15 Domain of the Human
... instead of 6 residues). The last domain of LEKTI is of particular interest, because of its partial homology to the only known natural occuring tryptase inhibitor LDTI, as well as the unequivocal correlation between the severe skin disorder disease Netherton Syndrome and defects in the gene encoding ...
... instead of 6 residues). The last domain of LEKTI is of particular interest, because of its partial homology to the only known natural occuring tryptase inhibitor LDTI, as well as the unequivocal correlation between the severe skin disorder disease Netherton Syndrome and defects in the gene encoding ...
Erlanson et al. PNAS - UCSF Macromolecular Structure Group
... and mono-N-(tert-butox ycarbonyl)-protected cystamine (mono-BOC-cystamine) by adapting the method of Parlow and coworkers (14). Briefly, 260 mol of each carboxylic acid was immobilized onto 130 mol equivalents of 4-hydroxy3-nitrobenzophenone on polystyrene resin using 1,3-diisopropylcarbodiimide ...
... and mono-N-(tert-butox ycarbonyl)-protected cystamine (mono-BOC-cystamine) by adapting the method of Parlow and coworkers (14). Briefly, 260 mol of each carboxylic acid was immobilized onto 130 mol equivalents of 4-hydroxy3-nitrobenzophenone on polystyrene resin using 1,3-diisopropylcarbodiimide ...
document
... – Ranges in color from brown to bright red – White poultry meat has low myoglobin – Dark meat has high myoglobin content – Veal and pork have less myoglobin than beef Myoglobin and hemoglobin without iron are colorless Myoglobin and hemoglobin with iron are pink to red Cooking meat dissociates heme ...
... – Ranges in color from brown to bright red – White poultry meat has low myoglobin – Dark meat has high myoglobin content – Veal and pork have less myoglobin than beef Myoglobin and hemoglobin without iron are colorless Myoglobin and hemoglobin with iron are pink to red Cooking meat dissociates heme ...
Curriculum Walk Through
... Basic content, lab techniques, and skills used by researchers in the biotechnology field Choice of lab sequences All specialized equipment provided including consumables at no cost Teachers provide limited, commonplace equipment (beakers, etc) ...
... Basic content, lab techniques, and skills used by researchers in the biotechnology field Choice of lab sequences All specialized equipment provided including consumables at no cost Teachers provide limited, commonplace equipment (beakers, etc) ...
Leukaemia Section t(12;21)(p12;q22) Atlas of Genetics and Cytogenetics
... Contains a HLH domain and a ETS-DNA binding domain; wide expression; nuclear localisation; ETSrelated transcription factor. ...
... Contains a HLH domain and a ETS-DNA binding domain; wide expression; nuclear localisation; ETSrelated transcription factor. ...
Mr. Tuan Hoang - Molecular and Cellular Biology
... central nervous system. However, with the exception of thermogenesis, no definite physiological role has been assigned to UCPs, and their structure and specific functions are poorly understood. The main goal of this study is to explore the structure and functional properties of mammalian UCPs, with ...
... central nervous system. However, with the exception of thermogenesis, no definite physiological role has been assigned to UCPs, and their structure and specific functions are poorly understood. The main goal of this study is to explore the structure and functional properties of mammalian UCPs, with ...
Adrenergic Receptor
... G protein-coupled receptors (GPCRs) are the largest family of integral membrane proteins coded by the human genome. GPCRs are important for signal transduction with the general structural characteristic of a plasma membrane receptor with seven transmembrane segments (Figure 1). One example of a GPCR ...
... G protein-coupled receptors (GPCRs) are the largest family of integral membrane proteins coded by the human genome. GPCRs are important for signal transduction with the general structural characteristic of a plasma membrane receptor with seven transmembrane segments (Figure 1). One example of a GPCR ...
Aromatic Amino Acids
... absorbance of ultraviolet light (ca. 280 nm) by proteins. Tyrosine is the only one of the aromatic amino acids with an ionizable side chain. Tyrosine is one of three hydroxyl containing amino acids. ...
... absorbance of ultraviolet light (ca. 280 nm) by proteins. Tyrosine is the only one of the aromatic amino acids with an ionizable side chain. Tyrosine is one of three hydroxyl containing amino acids. ...
Polar location and functional domains of the Agrobacterium
... probably forms an oligomeric complex. The formation of the complex will require VirD4 to be involved in homotypic interactions. Therefore, VirD4 should contain selfinteraction domains. We tested this hypothesis using the two-hybrid assay in yeast (Das and Xie, 2000). Owing to its large size (656 ami ...
... probably forms an oligomeric complex. The formation of the complex will require VirD4 to be involved in homotypic interactions. Therefore, VirD4 should contain selfinteraction domains. We tested this hypothesis using the two-hybrid assay in yeast (Das and Xie, 2000). Owing to its large size (656 ami ...
Chapter 14: Carbohydrates
... A single protein molecule contains hundreds or even thousands of amino acid units. An animal body has tens thousands of different proteins ...
... A single protein molecule contains hundreds or even thousands of amino acid units. An animal body has tens thousands of different proteins ...
Mar. 31 Presentation Phage Display
... Can apply standard affinity techniques to capture phage by taking advantage of displayed proteins. Pass solutions of amplified phages over solid support with antigens or receptors bound to it. Phages with affinity to support bind. ...
... Can apply standard affinity techniques to capture phage by taking advantage of displayed proteins. Pass solutions of amplified phages over solid support with antigens or receptors bound to it. Phages with affinity to support bind. ...
Biotechnology Lab (Kallas)
... Experiments & projects: We will begin the semester with an experiment to introduce a plasmid (pOSH37/GFP, which encodes an engineered “fusion” protein containing parts of the proteins thioredoxin, the jellyfish Green Fluorescent Protein, and an iron-sulfur protein), into a bacterial expression strai ...
... Experiments & projects: We will begin the semester with an experiment to introduce a plasmid (pOSH37/GFP, which encodes an engineered “fusion” protein containing parts of the proteins thioredoxin, the jellyfish Green Fluorescent Protein, and an iron-sulfur protein), into a bacterial expression strai ...
Fusion protein
... – may effect biological functioning – may make it unsuitable for clinical use ...
... – may effect biological functioning – may make it unsuitable for clinical use ...
Recombinant DNA Technology Manipulation of Gene Expression in
... – may effect biological functioning – may make it unsuitable for clinical use ...
... – may effect biological functioning – may make it unsuitable for clinical use ...
Recombinant DNA Technology Manipulation of Gene Expression in
... – may effect biological functioning – may make it unsuitable for clinical use ...
... – may effect biological functioning – may make it unsuitable for clinical use ...
Steps in Protein Sequencing Separate Fragments and Sequence
... • Automated Edman degradation generally used to sequence the individual fragments. – (Sometimes it may not be necessary to separate a few peptides before carrying out the automated sequencing. See your extra credit problem.) ...
... • Automated Edman degradation generally used to sequence the individual fragments. – (Sometimes it may not be necessary to separate a few peptides before carrying out the automated sequencing. See your extra credit problem.) ...
Document
... Tertiary - final folded shape of globular protein (3-dimensional shape) based on bonding of side groups Domains – independent functional units of the protein 100–200 amino acids long encoded by a specific DNA sequence (exon) Quaternary - forms when two or more polypeptide chains associate to form a ...
... Tertiary - final folded shape of globular protein (3-dimensional shape) based on bonding of side groups Domains – independent functional units of the protein 100–200 amino acids long encoded by a specific DNA sequence (exon) Quaternary - forms when two or more polypeptide chains associate to form a ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.