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Life and Chemistry: Large molecules: Proteins
Life and Chemistry: Large molecules: Proteins

... • Sometimes the parallel regions are in the same peptide, sometimes the parallel regions are from different peptide strands. • This sheet-like structure is stabilized by hydrogen bonds between N-H groups on one chain with the C=O group on the other. • Spider silk is made of b pleated sheets from sep ...
bZip Transcription factors: Picking up DNA with chopsticks
bZip Transcription factors: Picking up DNA with chopsticks

... promoter  regions  of  genes  to  control  their  expression.  As   such,  bZips  are  involved  in  numerous  fundamental  cellular  processes  and  many  are  implicated  in  cancer.  The activator  protein  1 (AP­1)  family for example, which contains the well known transcription factors c­Jun  a ...
Insights From The Molecular Docking Of
Insights From The Molecular Docking Of

... 9], DNA binding proteins [2, 4, 10], Rhodopsin family [11], metal binding proteins [12], sugar metabolizing proteins [13] and receptors [14] carrying out complex functions. However, the precise function of these palindromic sequences has not yet been fully understood. Palindromic sequences in protei ...
Structure and Function of Salivary Proteins Outline Basic salivary
Structure and Function of Salivary Proteins Outline Basic salivary

... • Difficulty in interpretation – Bioinformatics ...
Archives and Information Retrieval
Archives and Information Retrieval

... • a method of determining what is the function of uncharacterized proteins translated from genomic or cDNA sequences. • a database of biologically significant sites • patterns formulated in such a way that with appropriate computational tools it can rapidly and reliably identify to which known famil ...
Supplementary Information
Supplementary Information

... Modeling was performed with the molecular modeling and drug design program WHAT IF (1) on a Silicon Graphics workstation (SGI, Sunnyvale, USA). Backbone atom superimpositions and figures were prepared with the program MOLMOL (2). 3D structures for p53 are available for several p53 stretches and doma ...
DR AMENA RAHIM BIOCHEMISTRY
DR AMENA RAHIM BIOCHEMISTRY

... threonine, cysteine, asparagine, and glutamine.The polarity of serine and threonine is contributed by their hydroxyl groups; that of cysteine by its sulfhydryl group; and that of asparagine and glutamine by their amide groups. ...
Solutions for Practice Problems for Molecular Biology, Session 3
Solutions for Practice Problems for Molecular Biology, Session 3

... with a G/C base pair. How would this mutation affect the sequence of the protein that is produced? The codon UAU encoded Tyr, but now it is UAG, a stop codon. The protein is truncated. h) A third mutation occurs which results in the substitution of the C/G base pair at position 42 (shown in bold ita ...
Initiation at Class I Promoters
Initiation at Class I Promoters

... 1. Promotes binding of TFIIB, which promotes recruitment of the other factors and RNAP. ...
Mechanisms of mitochondrial protein import
Mechanisms of mitochondrial protein import

... residues; they usually lack acidic amino acids. Comparison of the primary structures of these signal sequences reveals no obvious homology or motif that might be responsible for the targeting function. These signal sequences, however, can adopt an amphiphilic -helix that might be important for thei ...
PowerPoint (click here)
PowerPoint (click here)

... 1. Go to UniProtKB (http://www.uniprot.org/) and search for the entry for human HEXA 2. What is the UniProtKB identifier for this protein? P06865 3. What is the function of this protein? Degrades GM2-gangliosides. A ganglioside is a type of glycolipid (sugar + lipid). 4. What disease is associated w ...
Genetic Techniques for Biological Research Chapter11
Genetic Techniques for Biological Research Chapter11

... the regulatory components in the complex. If the other components are made in limiting amounts, then the overproduced mutant subunit will bind to and sequester (titrate) the available subunits and make them unavailable to the small amount of wild-type protein synthesized by the otherwise wild-type h ...
Lecture 6: Peptides
Lecture 6: Peptides

... purify. • Proteins may be obtained from an original source (tissues from specific organism, yeast, etc.) • Proteins may also be obtained from recombinant organisms (clones) that overexpress the gene encoding the protein of interest. • In E. coli and yeast recombinant protein may constitute up to 30% ...
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... Can ocuurs spontaneously or brought about by external agents ...
The structure and function of proline
The structure and function of proline

... of a PRR containing tandemly repeated proline-rich sequences to several other PRRs. The better characterized is RNA polymerase II, which contains 26 or 27 nearly identical copies of the sequence YSPTSPS, in a presumably extended C-terminal domain. There is good evidence that it interacts with the TF ...
ATP Biochemistry: The Chemical Composition of Living Matter
ATP Biochemistry: The Chemical Composition of Living Matter

... Enzymes (catalysts) are proteins that catalyze biological reactions. Substrate = substance to be changed ...
Protein Structure - Oregon State University
Protein Structure - Oregon State University

... Cysteine and alanine 
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Bioch-301.NMR.2 - Vanderbilt Center for Structural Biology
Bioch-301.NMR.2 - Vanderbilt Center for Structural Biology

... Proteins Have Too Many Signals! 1H ...
Course Syllabus AG 408 – Nutritional Biochemistry Spring Semester, 2013 MWF 12:00-12:50
Course Syllabus AG 408 – Nutritional Biochemistry Spring Semester, 2013 MWF 12:00-12:50

... Attendance will be taken at the beginning of each lecture period, and will count for 10% of your final grade. There are 100 attendance points available, and you will lose 5 points for every unexcused absence. I expect that when you are in attendance you will not cause disruption, talk to your neighb ...
Abstract til NSKE 15
Abstract til NSKE 15

... Rationale: Dysmotility, nausea and vomiting are common among children with cerebral palsy (CP), but data on this issue is scarce. Aim: To examine the influence of protein composition on rate of gastric emptying and gastric electrical activity. Methods: 15 children with CP, mean age 10.7 years (range ...
Notes Guide Part 2
Notes Guide Part 2

... and –COOH (Carboxyl Group) ...
Distinct Roles of Alpha/Beta Hydrolase Domain Containing Proteins
Distinct Roles of Alpha/Beta Hydrolase Domain Containing Proteins

... Received: September 23, 2016; Accepted: November 25, 2016; Published: November ...
Center for Structural Biology
Center for Structural Biology

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The antibody validation experiments were essentially performed as
The antibody validation experiments were essentially performed as

... 6. The cells were seeded (3500 cells per well) and incubated at 37°C in humidified air with 5.2% CO2, for 72 h. 7. The growth medium was removed and 40 µl lysis buffer (150 mM NaCl, 50 mM Tris pH 8, 1% Triton X-100, 0.5% Sodium deoxycholate, 0.1% SDS) was added to each well. The plate was then incub ...
Mechanism Of Hormonal Action:3
Mechanism Of Hormonal Action:3

... factors and ribosomal subunits leading to alteration of protein transcription …. which mediate the effects of the hormone.  RAS is a G protein with intrinsic GTPase activity.  The other are serine/threonine kinases. Dr. Hani Alrefai - 2014 ...
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Protein–protein interaction



Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.
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