Polycomb Group silencers collaborate with Notch pathway to cause
... Mutant discs are oddly shaped when compared with WT discs, but the resulting adults have largely normal eyes. RT-PCR experiments were performed using RNA isolated from third instar larvae collected from GS88A8/hsp70-Gal4 heat-shocked (+) or without heat-shock (—). We noted that RT-PCR experiments sh ...
... Mutant discs are oddly shaped when compared with WT discs, but the resulting adults have largely normal eyes. RT-PCR experiments were performed using RNA isolated from third instar larvae collected from GS88A8/hsp70-Gal4 heat-shocked (+) or without heat-shock (—). We noted that RT-PCR experiments sh ...
The evolutionary ecology of senescence
... to future generations. Medawar supposed that deleterious mutations expressed at older ages would accumulate in populations and reduce the survival and reproductive success of older individuals. Williams extended this idea in a second theory by proposing the existence of antagonistically pleiotropic ...
... to future generations. Medawar supposed that deleterious mutations expressed at older ages would accumulate in populations and reduce the survival and reproductive success of older individuals. Williams extended this idea in a second theory by proposing the existence of antagonistically pleiotropic ...
DNA Hybridization: A Decade of Molecular Discourse in Hominoid
... hybridization that presented a complete matrix of distance values based on the average of five or more comparisons for each node, and it was also the first to present molecular evidence for a Pan-Homo clade. Based on the 183 DNA hybrids that were formed, the researchers interpreted the evidence to m ...
... hybridization that presented a complete matrix of distance values based on the average of five or more comparisons for each node, and it was also the first to present molecular evidence for a Pan-Homo clade. Based on the 183 DNA hybrids that were formed, the researchers interpreted the evidence to m ...
Aging, Theories of
... senescence to be primarily the result of random damage to the organism, or they are programmed theories that hold that senescence is the result of genetically determined processes. Currently most popular theories include: (a) the free radical theory, which holds that various reactive oxygen metaboli ...
... senescence to be primarily the result of random damage to the organism, or they are programmed theories that hold that senescence is the result of genetically determined processes. Currently most popular theories include: (a) the free radical theory, which holds that various reactive oxygen metaboli ...
number of fifty human tumours
... leucocytes), we find a mean DNA/chromosome ratio of 1-026 (standard deviation ±0 042). On the other hand 4 out of the 5 tumours (No. 1, 2, 18, 21 and 27) which were either metastases or recurrences following treatment had DNA/ chromosome ratios greater than 1-1. The DNA/chromosome ratios are shown g ...
... leucocytes), we find a mean DNA/chromosome ratio of 1-026 (standard deviation ±0 042). On the other hand 4 out of the 5 tumours (No. 1, 2, 18, 21 and 27) which were either metastases or recurrences following treatment had DNA/ chromosome ratios greater than 1-1. The DNA/chromosome ratios are shown g ...
DNA Testing - Who Murdered Robert Wone
... The restriction enzyme cuts the DNA into thousands of fragments of nearly all possible sizes. The sample is then electrophoretically separated. The DNA at this point is invisible in the gel unless the DNA is stained with a dye. A replica of the gel's DNA is made on something called a blot (also cal ...
... The restriction enzyme cuts the DNA into thousands of fragments of nearly all possible sizes. The sample is then electrophoretically separated. The DNA at this point is invisible in the gel unless the DNA is stained with a dye. A replica of the gel's DNA is made on something called a blot (also cal ...
reproductive cell fate transition in plants - Development
... at 18-20°C in a plant growth chamber or greenhouse, except for the mutants ago9-4, sgs3-11 and rdr6-2 (Olmedo-Monfil et al., 2010), which were grown at 23°C in a growth incubator (Percival). The GFP lines shown Fig. 2 and supplementary material Fig. S1 are the following: HTR5-GFP is pHTR5::HTR5-GFP ...
... at 18-20°C in a plant growth chamber or greenhouse, except for the mutants ago9-4, sgs3-11 and rdr6-2 (Olmedo-Monfil et al., 2010), which were grown at 23°C in a growth incubator (Percival). The GFP lines shown Fig. 2 and supplementary material Fig. S1 are the following: HTR5-GFP is pHTR5::HTR5-GFP ...
CpG Mutation Rates in the Human Genome Are
... SNPs that were produced by a CpG ! TpG mutation can be distinguished from SNPs that were caused by the reverse mutation (TpG ! CpG) by using the chimpanzee as an out-group to determine which of the two polymorphic bases was ancestral. We found 18,801 SNPs in human single-copy DNA that had a known an ...
... SNPs that were produced by a CpG ! TpG mutation can be distinguished from SNPs that were caused by the reverse mutation (TpG ! CpG) by using the chimpanzee as an out-group to determine which of the two polymorphic bases was ancestral. We found 18,801 SNPs in human single-copy DNA that had a known an ...
reproductive cell fate transition in plants - Development
... at 18-20°C in a plant growth chamber or greenhouse, except for the mutants ago9-4, sgs3-11 and rdr6-2 (Olmedo-Monfil et al., 2010), which were grown at 23°C in a growth incubator (Percival). The GFP lines shown Fig. 2 and supplementary material Fig. S1 are the following: HTR5-GFP is pHTR5::HTR5-GFP ...
... at 18-20°C in a plant growth chamber or greenhouse, except for the mutants ago9-4, sgs3-11 and rdr6-2 (Olmedo-Monfil et al., 2010), which were grown at 23°C in a growth incubator (Percival). The GFP lines shown Fig. 2 and supplementary material Fig. S1 are the following: HTR5-GFP is pHTR5::HTR5-GFP ...
The Relationship Between DNA Replication and the
... DNA, the fact that a segment of the genetic material in this region is non-isogenic with the remainder of the chromosome could reduce the efficiency of transformation for the metB gene. In this way it is possible to account for high values for the ratio of origin to terminus markers in DNA extracted ...
... DNA, the fact that a segment of the genetic material in this region is non-isogenic with the remainder of the chromosome could reduce the efficiency of transformation for the metB gene. In this way it is possible to account for high values for the ratio of origin to terminus markers in DNA extracted ...
Genomic approaches for the understanding of aging
... another important tissue in Drosophila melanogaster, the thorax, also results in the induction of genes involved in the immune response (40). In addition, genes linked to cellular morphogenesis and the proteasome complex are induced in the aged thorax. Down-regulated genes upon aging in the thorax a ...
... another important tissue in Drosophila melanogaster, the thorax, also results in the induction of genes involved in the immune response (40). In addition, genes linked to cellular morphogenesis and the proteasome complex are induced in the aged thorax. Down-regulated genes upon aging in the thorax a ...
Species Selection in Comparative Studies of Aging and Antiaging
... employ demographic measurements of aging. Aging can also be defined as an age-related increase in vulnerability and decrease in viability (Comfort, 1964). One of the features of aging in species with gradual senescence, like most mammals, is an exponential increase in mortality after maturity. For ex ...
... employ demographic measurements of aging. Aging can also be defined as an age-related increase in vulnerability and decrease in viability (Comfort, 1964). One of the features of aging in species with gradual senescence, like most mammals, is an exponential increase in mortality after maturity. For ex ...
Maintenance of DNA Methylation during the Arabidopsis Life Cycle
... FIS2 maternal allele in the endosperm. A putative general paternal silencing has been observed for reporters of endospermexpressed genes for up to 4 DAP (Vielle-Calzada et al., 2000). It could be hypothesized that such a general mechanism also affects expression at genomic loci for genes expressed i ...
... FIS2 maternal allele in the endosperm. A putative general paternal silencing has been observed for reporters of endospermexpressed genes for up to 4 DAP (Vielle-Calzada et al., 2000). It could be hypothesized that such a general mechanism also affects expression at genomic loci for genes expressed i ...
Adult Learners Presentation
... Loss of firmness along the jaw line and around the eyes. • Enlarged pores. • Clusters of irregular pigmentation often referred to as age spots. ...
... Loss of firmness along the jaw line and around the eyes. • Enlarged pores. • Clusters of irregular pigmentation often referred to as age spots. ...
Sheared DNA fragment sizing: comparison of techniques
... the relative number of DNA molecules corresponding to given length increments (base pairs) as determined by Kleinschmidt EM, Adsorption EM and gel electrophoresis procedures, respectively. Panels d, e and f show the relative mass of DNA at each length increment as determined respectively by the Klei ...
... the relative number of DNA molecules corresponding to given length increments (base pairs) as determined by Kleinschmidt EM, Adsorption EM and gel electrophoresis procedures, respectively. Panels d, e and f show the relative mass of DNA at each length increment as determined respectively by the Klei ...
Mapping the histone code at hMLH1. - JScholarship
... agent 5-Aza-2’deoxycytidine (5-Aza-dC), but not the histone deacetylase (HDAC) inhibitor Trichostatin A (TSA), reactivates the expression of such genes (Cameron et al., 1999; Suzuki et al., 2002). We now provide evidence that promoter DNA hypermethylation can control transcriptional silencing and, e ...
... agent 5-Aza-2’deoxycytidine (5-Aza-dC), but not the histone deacetylase (HDAC) inhibitor Trichostatin A (TSA), reactivates the expression of such genes (Cameron et al., 1999; Suzuki et al., 2002). We now provide evidence that promoter DNA hypermethylation can control transcriptional silencing and, e ...
word
... Genetic and epigenetic regulators of the germ line Specification of primordial germ cells (PGCs) in mammals occurs according to the 'stem cell model' in which pluripotent stem cells give rise to both the PGCs and somatic cells in response to signaling molecules. Expression of a transcriptional repre ...
... Genetic and epigenetic regulators of the germ line Specification of primordial germ cells (PGCs) in mammals occurs according to the 'stem cell model' in which pluripotent stem cells give rise to both the PGCs and somatic cells in response to signaling molecules. Expression of a transcriptional repre ...
Modeling Spatial Correlation of DNA Deformation
... extensions of DNA molecules subject to external forces, the model shows a very good agreement between theory and experiment with lP ∼ 150 bps ∼ 50 nm for double-stranded DNA under physiological conditions18 as well as in a flow field.19 Detailed variations of this model have been proposed over the yea ...
... extensions of DNA molecules subject to external forces, the model shows a very good agreement between theory and experiment with lP ∼ 150 bps ∼ 50 nm for double-stranded DNA under physiological conditions18 as well as in a flow field.19 Detailed variations of this model have been proposed over the yea ...
Epigenetic inheritance of acquired traits through sperm RNAs and
... spermatogenesis in the testis, which is followed by matur ation in the epididymis; each stage involves complex Box 2 | Transvection and paramutation in mice Transvection occurs during chromosome pairing such as during the chromosome synapsis stage of male meiosis in mice, in which an epigenetic mo ...
... spermatogenesis in the testis, which is followed by matur ation in the epididymis; each stage involves complex Box 2 | Transvection and paramutation in mice Transvection occurs during chromosome pairing such as during the chromosome synapsis stage of male meiosis in mice, in which an epigenetic mo ...
KAIE LOKK Comparative genome-wide DNA methylation
... The human genome contains about 29 million CpG dinucleotides where a cytosine base is followed by a guanine base. Cytosine in the CpG dinucleotide can exist in either a methylated or unmethylated state. DNA methyltransferase (DNMT) enzymes can add a methyl group to cytosine to form 5-methylcytosine ...
... The human genome contains about 29 million CpG dinucleotides where a cytosine base is followed by a guanine base. Cytosine in the CpG dinucleotide can exist in either a methylated or unmethylated state. DNA methyltransferase (DNMT) enzymes can add a methyl group to cytosine to form 5-methylcytosine ...
Supercoils in plant DNA: nucleoid
... development and drying (Bewley & Black, 1978). Our flow cytometric analysis of dry embryo nuclear populations confirmed literature data in that both ZC and 4C nuclei were present. The proportion of 4C nuclei was much higher than that in the meristem (not shown). Again, no nuclei of higher than AC DN ...
... development and drying (Bewley & Black, 1978). Our flow cytometric analysis of dry embryo nuclear populations confirmed literature data in that both ZC and 4C nuclei were present. The proportion of 4C nuclei was much higher than that in the meristem (not shown). Again, no nuclei of higher than AC DN ...
The distribution of DNA translocation times in solid
... membrane, a stable open pore current I0 will be observed. After adding negatively charged DNA molecules to the cis chamber, the molecules in the vicinity of the nanopore will be captured by the electric field and forced to pass through the nanopore to the positively biased trans chamber. The translo ...
... membrane, a stable open pore current I0 will be observed. After adding negatively charged DNA molecules to the cis chamber, the molecules in the vicinity of the nanopore will be captured by the electric field and forced to pass through the nanopore to the positively biased trans chamber. The translo ...
S4 Text.
... These are excerpts from the lab manual written by the instructor for use in our course (omitted pages/sections feature course-specific information not needed to carry out these experiments at a different institution). The two modules - 1) inverse PCR using genomic DNA extracted from adult flies for ...
... These are excerpts from the lab manual written by the instructor for use in our course (omitted pages/sections feature course-specific information not needed to carry out these experiments at a different institution). The two modules - 1) inverse PCR using genomic DNA extracted from adult flies for ...
Electrokinetic Stretching of Tethered DNA
... EOF was negligible, no precautions were taken to reduce this flow. Since their experiments were performed on a silicon surface, which readily develops a surface charge in water, EOF would certainly be present at the ionic strengths used in their experiments (0.53 TBE), and interfere with the interpr ...
... EOF was negligible, no precautions were taken to reduce this flow. Since their experiments were performed on a silicon surface, which readily develops a surface charge in water, EOF would certainly be present at the ionic strengths used in their experiments (0.53 TBE), and interfere with the interpr ...