Physiological characterization of natural transformation in
... regulation of competence development in Bacillus subtilis (Dubnau, 1991). These differences suggest that not only the mechanism of DNA uptake in natural transformation differs among these bacteria, but that possibly its function varies also in different species. Acinetobacter calcoaceticus, a Gram-n ...
... regulation of competence development in Bacillus subtilis (Dubnau, 1991). These differences suggest that not only the mechanism of DNA uptake in natural transformation differs among these bacteria, but that possibly its function varies also in different species. Acinetobacter calcoaceticus, a Gram-n ...
Fulltext PDF - Indian Academy of Sciences
... basis of the allelic variation. Most early-flowering ecotypes carry FRI alleles that contain one of two different deletions that disrupt the open reading frame of the FRI gene. This suggests that loss-offunction mutations at FRI have provided the basis for the evolution of many early-flowering Arabi ...
... basis of the allelic variation. Most early-flowering ecotypes carry FRI alleles that contain one of two different deletions that disrupt the open reading frame of the FRI gene. This suggests that loss-offunction mutations at FRI have provided the basis for the evolution of many early-flowering Arabi ...
Modeling Fragile X Syndrome Using Human Pluripotent Stem Cells
... increasingly unstable, resulting in high degree of variation in expansion size between and within tissues of affected individuals. It is still unclear how the FMR1 protein (FMRP) deficiency leads to disease pathology in neurons. Nor do we know the mechanisms by which the CGG expansion results in abe ...
... increasingly unstable, resulting in high degree of variation in expansion size between and within tissues of affected individuals. It is still unclear how the FMR1 protein (FMRP) deficiency leads to disease pathology in neurons. Nor do we know the mechanisms by which the CGG expansion results in abe ...
The presence of two UvrB subunits in the UvrAB complex ensures
... is expected to cause local melting of the DNA helix, thereby facilitating insertion of the b-hairpin of UvrB between the DNA strands. From mutational analysis of the b-hairpin it was proposed that UvrB scans the DNA for damage by trying to ¯ip nucleotides out of the DNA helix, thereby probing for di ...
... is expected to cause local melting of the DNA helix, thereby facilitating insertion of the b-hairpin of UvrB between the DNA strands. From mutational analysis of the b-hairpin it was proposed that UvrB scans the DNA for damage by trying to ¯ip nucleotides out of the DNA helix, thereby probing for di ...
CHAPTER 14 DNA applications in society
... Advantages of producing proteins through expression of cloned genes as compared to extraction from other biological sources include: • high levels of purity • reliability of supply • consistency of quality between batches. The following section identifies a tragic problem that emerged when the on ...
... Advantages of producing proteins through expression of cloned genes as compared to extraction from other biological sources include: • high levels of purity • reliability of supply • consistency of quality between batches. The following section identifies a tragic problem that emerged when the on ...
Agarose Gel Electrophoresis
... Parameters affecting the migration of DNA through agarose gels Agarose concentration. Molecules of linear, duplex DNA (form III) travel through gel matrices at a rate that is inversely proportional to the log10 of their molecular weight (Helling et al., 1974). The molecular weight of a fragment of i ...
... Parameters affecting the migration of DNA through agarose gels Agarose concentration. Molecules of linear, duplex DNA (form III) travel through gel matrices at a rate that is inversely proportional to the log10 of their molecular weight (Helling et al., 1974). The molecular weight of a fragment of i ...
Identification of Genetic and Epigenetic Risk Factors for Psoriasis
... of the European population. ~10–30% of patients develop psoriatic arthritis (PsA). Genetic variation in the major histocompatibility complex (MHC) increases risk of developing PS. However, only ~10% of individuals with this risk factor develop PS, indicating that other genetic effects and environmen ...
... of the European population. ~10–30% of patients develop psoriatic arthritis (PsA). Genetic variation in the major histocompatibility complex (MHC) increases risk of developing PS. However, only ~10% of individuals with this risk factor develop PS, indicating that other genetic effects and environmen ...
Agarose Gel Electrophoresis
... The intercalating dye, ethidium bromide, is commonly incorporated into the gel and running buffer. The dye reduces the mobility of linear duplexes (Fig. 10.4.2D) and has a particularly pronounced effect on the mobility of closed circular DNA. Ethidium bromide changes the superhelical density of clos ...
... The intercalating dye, ethidium bromide, is commonly incorporated into the gel and running buffer. The dye reduces the mobility of linear duplexes (Fig. 10.4.2D) and has a particularly pronounced effect on the mobility of closed circular DNA. Ethidium bromide changes the superhelical density of clos ...
Diversity of DNA methyltransferases that recognize asymmetric
... 1997), and M.MboIIA (Osipiuk et al., 2003) were crystallized as protein dimers. However, further biochemical and structural studies are required to confirm the relevance of this dimerization. The extent of subunit contacts observed in the crystal structure of M.MboIIA suggested that the two molecule ...
... 1997), and M.MboIIA (Osipiuk et al., 2003) were crystallized as protein dimers. However, further biochemical and structural studies are required to confirm the relevance of this dimerization. The extent of subunit contacts observed in the crystal structure of M.MboIIA suggested that the two molecule ...
Identifying the genomic determinants of aging and longevity in
... have only identified one genome-wide significant ( p < 5 108) locus: APOE, which has long been established as a longevity gene. Several other loci showed suggestive association with longevity ( p < 5 106), namely MINPP1 [38], OTOL1 [39], and CAMKIV [42] (Fig. 3). However, the effect of these l ...
... have only identified one genome-wide significant ( p < 5 108) locus: APOE, which has long been established as a longevity gene. Several other loci showed suggestive association with longevity ( p < 5 106), namely MINPP1 [38], OTOL1 [39], and CAMKIV [42] (Fig. 3). However, the effect of these l ...
Biogenesis and significance of lipofuscin in the equine thyroid gland
... long-lived cells of many eucaryotes, including man. The granules are derived from lysosomes, and are defined by characteristic morphology, colour, fluorescence, and histochemistry. They increase in number with age in post-mitotic cells, and are regarded as markers of the aging process. Lipofuscin is ...
... long-lived cells of many eucaryotes, including man. The granules are derived from lysosomes, and are defined by characteristic morphology, colour, fluorescence, and histochemistry. They increase in number with age in post-mitotic cells, and are regarded as markers of the aging process. Lipofuscin is ...
Pierre MADL Div. of Material Sciences Dep
... Conrad Waddington (1905-1975) is often credited with coining the term epigenetics in 1942 as “the branch of biology which studies the causal interactions between genes and their products, which bring the phenotype into being”. Epigenetics appears in the literature as far back as the mid 19th century ...
... Conrad Waddington (1905-1975) is often credited with coining the term epigenetics in 1942 as “the branch of biology which studies the causal interactions between genes and their products, which bring the phenotype into being”. Epigenetics appears in the literature as far back as the mid 19th century ...
GENECLEAN® Kit
... When working with radio-labeled DNA, add 1 µl of Label Block to each 10 µl of GLASSMILK® and incubate for 5 minutes at room temperature before adding to the DNA/NaI solution in the next step. 5. Add GLASSMILK® to the DNA/NaI solution as calculated in Step 3. Vortex gently or stir with a pipet tip to ...
... When working with radio-labeled DNA, add 1 µl of Label Block to each 10 µl of GLASSMILK® and incubate for 5 minutes at room temperature before adding to the DNA/NaI solution in the next step. 5. Add GLASSMILK® to the DNA/NaI solution as calculated in Step 3. Vortex gently or stir with a pipet tip to ...
Effects of Salt Concentrations and Bending Energy on the Extent of
... therefore a measure of the stressed state of the DNA, due to both its crowding and bending. It has recently been shown that this force can be determined by experiments in which osmotic pressure is employed to inhibit the ejection (6). The results of these studies are consistent with the predictions ...
... therefore a measure of the stressed state of the DNA, due to both its crowding and bending. It has recently been shown that this force can be determined by experiments in which osmotic pressure is employed to inhibit the ejection (6). The results of these studies are consistent with the predictions ...
The legal, social and ethical controversy of the collection and
... can come from any tissue of the body [7, p.1]. DNA fingerprinting was discovered in 1985 by English geneticist Dr Alec Jeffreys. He found that certain regions of DNA contained sequences that repeated themselves over and over again, one after the other and that different individuals had a different n ...
... can come from any tissue of the body [7, p.1]. DNA fingerprinting was discovered in 1985 by English geneticist Dr Alec Jeffreys. He found that certain regions of DNA contained sequences that repeated themselves over and over again, one after the other and that different individuals had a different n ...
Chromatin DNA Methylayion
... Model 2: The steady state methylation pattern is a dynamic equilibrium between methylase and demethylase activities CH3 ...
... Model 2: The steady state methylation pattern is a dynamic equilibrium between methylase and demethylase activities CH3 ...
Homologous Recombination Generates T-Loop
... Lange, 2002). Dwindling presence of the TRF2 complex on shortening telomeres is a likely cause of telomere dysfunction (Karlseder et al., 2002). Like telomeres that are depleted of TRF2, shortened telomeres become associated with DNA damage response factors (e.g., 53BP1), forming cytological structu ...
... Lange, 2002). Dwindling presence of the TRF2 complex on shortening telomeres is a likely cause of telomere dysfunction (Karlseder et al., 2002). Like telomeres that are depleted of TRF2, shortened telomeres become associated with DNA damage response factors (e.g., 53BP1), forming cytological structu ...
Activity Name - Science4Inquiry.com
... Something Old, Something New… You are a Molecular Biologist who is studying the process of DNA replication. Your task is to build a model that represents this process. Next you will investigate where DNA replication took place in the human body and explain why it occurred. This activity is divided u ...
... Something Old, Something New… You are a Molecular Biologist who is studying the process of DNA replication. Your task is to build a model that represents this process. Next you will investigate where DNA replication took place in the human body and explain why it occurred. This activity is divided u ...
Quantifying the DNA Binding Properties of the Binuclear Ruthenium
... academic career at BSU. I have been tremendously fortunate to have him as a mentor. He has spent an unbelievable amount of time helping me improve myself academically and I have him to thank for all that I’ve accomplished. I admire nothing more than his dedication to his students and I hope to one d ...
... academic career at BSU. I have been tremendously fortunate to have him as a mentor. He has spent an unbelievable amount of time helping me improve myself academically and I have him to thank for all that I’ve accomplished. I admire nothing more than his dedication to his students and I hope to one d ...
Evolutionary Origin and Adaptive Function of Meiosis
... 2001). The oldest taxonomically resolved eukaryote in the fossil record, Bangiomorph pubes‐ cens, a red algae, existed more than 1.2 billion years ago and was sexually reproducing (Butterfield, 2000). Although meiotic sex is widespread among extant eukaryotes, it has, until recently, been unclear wh ...
... 2001). The oldest taxonomically resolved eukaryote in the fossil record, Bangiomorph pubes‐ cens, a red algae, existed more than 1.2 billion years ago and was sexually reproducing (Butterfield, 2000). Although meiotic sex is widespread among extant eukaryotes, it has, until recently, been unclear wh ...
Topologically Non-linked Circular Duplex DNA
... inverted in aqueous methanol solutions. They suggested that this was best explained as an R → L transition, although there are other possible explanations. Similar inversions were demonstrated in the circular dichroism (CD) spectra of DNA at high salt concentration (Zimmer and Luck, 1974), and follo ...
... inverted in aqueous methanol solutions. They suggested that this was best explained as an R → L transition, although there are other possible explanations. Similar inversions were demonstrated in the circular dichroism (CD) spectra of DNA at high salt concentration (Zimmer and Luck, 1974), and follo ...
Gene methylation in gastric cancer
... aberrant methylation of several tumor-related genes is significantly associated with the age in gastric cancer, such as CDH1 and DAPK [31]. However, it is noteworthy that age-related methylation of tumor-suppressor genes applies mostly to exonic or far upstream regions within a promoter CGI, and that ...
... aberrant methylation of several tumor-related genes is significantly associated with the age in gastric cancer, such as CDH1 and DAPK [31]. However, it is noteworthy that age-related methylation of tumor-suppressor genes applies mostly to exonic or far upstream regions within a promoter CGI, and that ...
Regulation and Flexibility of Genomic Imprinting
... and thus contributes two maternal genomes to the triploid endosperm, whereas only one genome is of paternal origin. This genetic peculiarity is of importance for seed formation and considerably complicates the interpretation of parent-of-origin– specific effects during seed development (reviewed in ...
... and thus contributes two maternal genomes to the triploid endosperm, whereas only one genome is of paternal origin. This genetic peculiarity is of importance for seed formation and considerably complicates the interpretation of parent-of-origin– specific effects during seed development (reviewed in ...
Extrachromosomal DNA Transformationof Caenorhabditis elegans
... with 32P-labeled pBR322 DNA, bacteriophage DNA containing a single-copy gene, and bacteriophage DNA containing a fourfold repetitive actin gene sequence (15) (generous gifts of M. Krause). After washing, the hybridization to individual spots was quantitated by liquid scintillation counting. To contr ...
... with 32P-labeled pBR322 DNA, bacteriophage DNA containing a single-copy gene, and bacteriophage DNA containing a fourfold repetitive actin gene sequence (15) (generous gifts of M. Krause). After washing, the hybridization to individual spots was quantitated by liquid scintillation counting. To contr ...
A Novel CpG Island Set Identifies Tissue-Specific
... DNA methyltransferases that are essential for development and viability [1,2]. The pattern of CpG methylation in the human genome distinguishes two fractions with distinct properties: a major fraction (;98%), in which CpGs are relatively infrequent (on average 1 per 100 bp) but highly methylated (ap ...
... DNA methyltransferases that are essential for development and viability [1,2]. The pattern of CpG methylation in the human genome distinguishes two fractions with distinct properties: a major fraction (;98%), in which CpGs are relatively infrequent (on average 1 per 100 bp) but highly methylated (ap ...