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"Ecophysiological and molecular characterization of estuarine microbial mats"  Laura VILLANUEVA ÁLVAREZ
"Ecophysiological and molecular characterization of estuarine microbial mats" Laura VILLANUEVA ÁLVAREZ

... extensively used to monitor bacterial communities in space and time (Ferris and Ward, 1997; Nübel et al., 1999) or to evaluate the impact of environmental disturbances (Müller et al., 2001). Recently, DNA-based and lipid analysis have been combined to study the community ecology of microorganisms to ...
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Phospholipid-derived fatty acids



Phospholipid-derived fatty acids (PLFA) are widely used in microbial ecology as chemotaxonomic markers of bacteria and other organisms. Phospholipids are the primary lipids composing cellular membranes. Phospholipids can be saponified, which releases the fatty acids contained in their diglyceride tail. Once the phospholipids of an unknown sample are saponified, the composition of the resulting PLFA can be compared to the PLFA of known organisms to determine the identity of the sample organism. PLFA analysis may be combined with other techniques, such as stable isotope probing to determine which microbes are metabolically active in a sample. PLFA analysis was pioneered by D.C. White, MD, PhD, at the University of Tennessee, in the early to mid 1980s.
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