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Basic Local Alignment Search Tool
Basic Local Alignment Search Tool

... The initial search is done for a word of length "W" that scores at least "T" when compared to the query using a substitution matrix. Word hits are then extended in either direction in an attempt to generate an alignment with a score exceeding the threshold of "S". The "T" parameter dictates the spee ...
王红刚
王红刚

... Thread the query sequence onto the template structures Improve prediction performance by either incorporating new features or developing novel algorithms. ...
The Maize Abscisic Acid-Responsive Protein Rabl7
The Maize Abscisic Acid-Responsive Protein Rabl7

... In an earlier study, we showed that the Rabl7 protein is the most heavily phosphorylated protein in the mature maize embryo (Goday et al., 1988). The predicted protein sequence of Rabl7 contains a cluster of eight serine residues followed by a consensus site for casein kinase II (CKII). Previously, ...
lecture 21
lecture 21

... Dr. Lynne Quarmby in MBB studies Katanin  Katanin is part of the large AAA ATPase family  heterodimer consisting of 60 kDa microtubule-stimulated ATPase that requires ATP hydrolysis to disassemble microtubules, and 80 kDa subunit that targets the complex to the centrosome and regulates the activit ...
Protein - HCC Learning Web
Protein - HCC Learning Web

... antibodies, and a variety of other proteins. Less frequently, proteins are long and fibrous and most of these elongated molecules are insoluble in water and serve a role in the maintenance of cell structure. The three-dimensional structure of a protein is due to the type and sequence of its constitu ...
The Quiet Revolution! - Montana State University
The Quiet Revolution! - Montana State University

... • March Women learns to recognize kwashiorkor and make intervention diet for at-risk children • Sept women discourage children’s grasshopper hunting and eating due to pesticides in nearby cotton fields • Sept students and mentors introduce brick wall concept of complete proteins ...
Mutation analysis of a recombinant NS replicon shows that influenza
Mutation analysis of a recombinant NS replicon shows that influenza

... capable of promoting viral RNA replication and antagonizing the host innate IFN response, while NEP pertains to assisting nucleo-cytoplasmic transport of viral RNPs and can be found as a minor structural protein in the virion. Recent evidence further demonstrates that NS1 negatively regulates NEP pr ...
Humans are living significantly longer than ever before, and
Humans are living significantly longer than ever before, and

... disorders, which despite having different clinical manifestations, share a similar pathology: the misfolding and aggregation of disease-specific proteins (Cushman et al., 2010; Liu et al., 2013). Therefore, a great emphasis has been made on possible therapies that can dissolve these aggregates and r ...
Differentially Expressed Soluble Proteins in Aortic Cells from
Differentially Expressed Soluble Proteins in Aortic Cells from

... Several differences between the proteomes of cells from the 2 breeds were observed. Most significant was the finding that some proteins unique to either the susceptible breed or the resistant breed migrated exclusively into specific zones when a map of MW vs. pI was plotted for the differentially ex ...
Architectural TFs
Architectural TFs

... conformation of DNA A single AT-hook preferentially binds to stretches of 4-6 bp of AT-rich sequence, and partially neutralizes the negatively charged backbone phosphates on only one face of the DNA helix.  The number and spacing of AT-rich binding sites in DNA influences the conformation of bound ...
Slide 1
Slide 1

... However, it is also known that certain sets of independent domains are frequently found together, which may indicate functional cooperation. Supra- Domains : A supra-domain is defined as a domain combination in a particular N-to-C-terminal orientation that occurs in at least two different domain arc ...
NAC Protein Family
NAC Protein Family

... • Def: Stem-cell tissue at the tip of the shoot or root that divides, and is responsible for increasing the plant’s length. Shoot AM --> Root AM --> ...
Host cell protein analysis by mass spectrometry and its application
Host cell protein analysis by mass spectrometry and its application

... HCP analysis in a comparability exercise  Case: Exchange of the depth filter in the manufacturing process  ELISA results showed comparable levels of HCP in the drug substance batches before and after the filter change ...
13.2 Ribosomes and Protein Synthesis
13.2 Ribosomes and Protein Synthesis

... The _______________ of nucleotide ____________ in an mRNA molecule is a set of _____________________ that gives the _____________ in which amino acids should be joined to produce a polypeptide. ...
What`s in YOUR protein bar?
What`s in YOUR protein bar?

... itself is a good protein source but very dry. To make these bars edible or to add moisture, animal by products are added in the form of “Hydrolyzed gelatin” or as it used to be labled till about 2004 “hydrolyzed bovine protein” or “hydrolyzed collagen”, which is a nicer way to say horse hooves. It c ...
Seasonal regulation of a 24-kDa protein from red
Seasonal regulation of a 24-kDa protein from red

... spots of interest were excised. Following acid hydrolysis (110 °C for 22 h), amino acids were derivatized with AccQFluor reagent (Waters Corporation, Milford, MA) and subsequently separated by AccQ-Tag (Waters Corporation) reverse phase column chromatography following the manufacturer’s instructions ...
Beef Cookery - Georgia Beef Board
Beef Cookery - Georgia Beef Board

...  Remove collagen if using dry heat  Elastin should be removed prior to cooking  Sarcoplasmic proteins  Water soluble protein  Hemoglobin and myoglobin  Responsible for beef’s red color ...
Structure studies of a protein: macromolecular crystallography (3D
Structure studies of a protein: macromolecular crystallography (3D

... atoms within a crystal, in which a beam of X-rays strikes a crystal and diffracts into many specific directions. From the angles and intensities of these diffracted beams, can be produced a three-dimensional picture of the density of electrons within the crystal. From this electron density, the mean ...
View/Download
View/Download

... Trends + Innovation ...
Milk Protein and Butterfat Values
Milk Protein and Butterfat Values

... High total diet intake has a positive influence on milk protein. ...
BioN03 Amino acids, peptides, proteins Summer 2015
BioN03 Amino acids, peptides, proteins Summer 2015

... The primary structure of a protein is the sequence of amino acid residues that constitute the polypeptide chain. Secondary structure refers to the localized organization of parts of a polypeptide chain. Tertiary structure refers to the three-dimensional structure of a polypeptide chain, that is, the ...
Carmyle and Kenmuir Mount Vernon Church`s Website article
Carmyle and Kenmuir Mount Vernon Church`s Website article

... assemble this one protein every single second from the 'Big Bang', how many proteins would be expected to be assembled? Well none actually. The best estimate of the number of fundamental particles in the entire universe is estimated to be 1085 and the number of seconds since the 'Big Bang' until now ...
Amino Acids as Protein Building Blocks
Amino Acids as Protein Building Blocks

... Amino acids are most logically grouped according to the physical properties of their side chains. ...
A quantitative atlas of mitotic phosphorylation
A quantitative atlas of mitotic phosphorylation

... phosphorylation regulates the cell cycle, we sought to identify proteins whose phosphorylation is cell cycle regulated. Using stable isotope labeling along with a two-step strategy for phosphopeptide enrichment and high mass accuracy mass spectrometry, we examined protein phosphorylation in a human ...
Lesson 15. Nutrition
Lesson 15. Nutrition

... Pulses are a rich source of proteins among vegetarian food. But similar to other vegetable proteins they are deficient in certain amino acids but these deficiencies can be overcome by supplementing them with cereals. They have a energy density of about 350Kcal/100 gm and 20% to 25% of their mass is ...
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Protein mass spectrometry



Protein mass spectrometry refers to the application of mass spectrometry to the study of proteins. Mass spectrometry is an important emerging method for the characterization of proteins. The two primary methods for ionization of whole proteins are electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). In keeping with the performance and mass range of available mass spectrometers, two approaches are used for characterizing proteins. In the first, intact proteins are ionized by either of the two techniques described above, and then introduced to a mass analyzer. This approach is referred to as ""top-down"" strategy of protein analysis. In the second, proteins are enzymatically digested into smaller peptides using a protease such as trypsin. Subsequently these peptides are introduced into the mass spectrometer and identified by peptide mass fingerprinting or tandem mass spectrometry. Hence, this latter approach (also called ""bottom-up"" proteomics) uses identification at the peptide level to infer the existence of proteins.Whole protein mass analysis is primarily conducted using either time-of-flight (TOF) MS, or Fourier transform ion cyclotron resonance (FT-ICR). These two types of instrument are preferable here because of their wide mass range, and in the case of FT-ICR, its high mass accuracy. Mass analysis of proteolytic peptides is a much more popular method of protein characterization, as cheaper instrument designs can be used for characterization. Additionally, sample preparation is easier once whole proteins have been digested into smaller peptide fragments. The most widely used instrument for peptide mass analysis are the MALDI time-of-flight instruments as they permit the acquisition of peptide mass fingerprints (PMFs) at high pace (1 PMF can be analyzed in approx. 10 sec). Multiple stage quadrupole-time-of-flight and the quadrupole ion trap also find use in this application.
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