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Molecular Diagnostics as an Indispensable Tool for the
Molecular Diagnostics as an Indispensable Tool for the

... nucleic acid tests in the diagnosis of infectious disease of viral etiology, considering that a critical step to proper treatment and control of any virus infection is a correct diagnosis. Diagnostic tests based on nucleic acid (NAT, nucleic acid tests) more used are the nucleic acid sequence-based ...
- Ex Student Archive
- Ex Student Archive

... With increasing industrialization and intensification of rearing systems the disease pattern in fowl are changing. There are increasing problems with nutritional and genetic diseases due to higher demands on growing and food conversion capacities. Outbreaks of infectious diseases are often due to th ...
Photochemical inactivation of chikungunya virus in plasma and
Photochemical inactivation of chikungunya virus in plasma and

... Another major weakness of screening assays is their ineffectiveness against emerging pathogens, since detection assays only work against the target agent for which they were designed. Once a new agent of concern has been identified, industry must determine if their investment costs for test developm ...
Virus Inactivation - Evaluation of Processes used in Biowaste
Virus Inactivation - Evaluation of Processes used in Biowaste

... Animal by-products (ABP) Category 3 includes hatchery waste, and also slaughterhouse waste and manure, aimed for use in biogas production. In order to be used as fertilisers, they must be sanitised to reduce pathogenic microorganisms. The initial European regulations regarding processing standards f ...
Surveillance of viral fish diseases in the Czech Republic over the
Surveillance of viral fish diseases in the Czech Republic over the

... (Table 2). Positive samples included tissue homogenates as well as ovarian fluids. The virus was isolated only once during the routine surveillance. The remaining positive cases were derived from diseased fish, despite the fact that the number of diagnostic samples was nearly 10 times lower than the ...
Understanding Microbes in Sickness and Health
Understanding Microbes in Sickness and Health

... contact with a cell it likes, it may attach to special landing sites on the surface of that cell. From there, the virus may inject molecules into the cell, or the cell may swallow the virion. Once inside the cell, viral molecules such as DNA or RNA direct the cell to make new virus offspring. That’s ...
mauritania national survey
mauritania national survey

... is transmitted through direct contact with infected blood or body fluids. A common misconception is that the virus can also be spread through air particles. To assess knowledge levels among Mauritanians, a multiple response question related to modes of Ebola transmission was administered to those na ...
OTOLOGY SEMINAR
OTOLOGY SEMINAR

... and respiratory syncytial virus by a time-resolved fluoroimmunoassay (TR-FIA) and human rhinovirus and human enterovirus by RT-PCR „ Respiratory virus was detected in 70% of NPA samples and in 27% of ...
English  - SciELO Costa Rica
English - SciELO Costa Rica

... under experimental conditions at 12°C, clinical disease occurs in salmonids, but possible explanations are unknown. As fish gain weight, they begin to be resistant to this disease and become asymptomatic carriers (Imajoh, Hirayama, & Oshima, 2005), representing a risk to wild fish populations and th ...
Chronic viral hepatitis and hepatitis B virus infection: stop the cancer
Chronic viral hepatitis and hepatitis B virus infection: stop the cancer

... turn become chronically infected, perpetuating the cycle of transmission. It had been proposed that the ability of the virus to persist in the newborn and infants is due to an immune tolerance caused by the secreted hepatitis B ‘e’ antigen (HBeAg), which can cross the placenta. Recent work has confir ...
Isolation and characterization of a rhabdovirus from starry flounder
Isolation and characterization of a rhabdovirus from starry flounder

... primer, but with reverse primer 59-TCAAAAGTCTCGTGAACTCT39 to amplify a 348 bp region. For both amplifications, DNA was analysed on a 1?5 % agarose gel and visualized by ethidium bromide staining. The visible PCR product from the second round was purified with a StrataPrep kit (Stratagene) and labell ...
Nipah virus conforms to the rule of six in a minigenome replication
Nipah virus conforms to the rule of six in a minigenome replication

... and the T7 promoter sequence was added to this fragment by reverse primer NGR6. NGR6 contained a HindIII restriction site, allowing the fragment to be inserted into pUC fragment 1 with BamHI and HindIII to produce pNiV-CAT. Additional nucleotides were added to primer NGR4 to generate plasmids encodi ...
A sandwich ELISA to detect VHSV and IPNV in turbot | SpringerLink
A sandwich ELISA to detect VHSV and IPNV in turbot | SpringerLink

... isolates. The epitopes defined by the chosen Mabs were non-competitive and highly conserved in the VHSV (Sanz et a/., 1991, results not published) and in the IPNV (Domfnguez et al., I991) serotypes. Furthermore, these proteins are a major component in both the complete virus and infected cells (Basu ...
Peer Immunisation Seasonal Influenza
Peer Immunisation Seasonal Influenza

... • This enable them to get vaccinated on site of their work without having to attend a mass vaccination or occupational health clinics • Staff working in the evenings, night shift and at weekends can also get vaccinated on ...
FACT SHEET – Pandemic Flu - Lake Cumberland District Health
FACT SHEET – Pandemic Flu - Lake Cumberland District Health

... If they have a fever, difficulty breathing, a cough, or any illness during this period, they should talk with a health care provider. ...
Communicable Diseases and Immunisation Guidelines
Communicable Diseases and Immunisation Guidelines

... Vaccinia is one of a family of Orthopoxviruses capable of causing disease in humans. These diseases include monkeypox, cowpox and smallpox. The vaccinia virus vaccine guards against all these diseases, even though they are caused by different viruses within the Orthopoxvirus family. The vaccinia vir ...
Sample pages 2 PDF
Sample pages 2 PDF

... sudden onset, edema in the skin of the head and proboscis, cyanosis of the tongue, decreased levels of white blood cells and platelets, and internal hemorrhages. Histologically, basophilic intranuclear inclusion bodies were detected in vascular cells of the heart, liver, and tongue. Death was found ...
Influenza Vaccine Facts - Quality Improvement Organizations
Influenza Vaccine Facts - Quality Improvement Organizations

... A flu vaccine can't give you the flu. The "stomach flu" and influenza are the same thing. Getting a flu vaccine in December or later is not too late. Flu viruses change constantly which requires a new flu vaccine to be produced each year. 5. Washing your hands is the best thing you can do to protect ...
ISOLATION AND IDENTIFICATION OF A BOVINE RESPIRATORY
ISOLATION AND IDENTIFICATION OF A BOVINE RESPIRATORY

... et al. (1976), on the other hand, found no evidence to indicate that the development of clinical symptoms depended on the presence of serum antibodies and concluded that in this respect bovine RSV differs from human RSV. Another difference between human RSVand bovine RSV infections were pointed out ...
Lecture 2 Evolution in action: the HIV virus
Lecture 2 Evolution in action: the HIV virus

... How does HIV evolve so rapidly? 1. High mutation rate • HIV’s mutation rate is 106 higher than ours! 2. Short generation time • 1 year ≅ 300 viral generations. 10 years of viral ≅ evolution ...
Dengue Virus
Dengue Virus

...  All efforts of control are aimed against mosquitoes  Elimination of breeding areas  Actions to prevent mosquito bites (repellant, nets, and ...
PDF version: RGF Test Booklet
PDF version: RGF Test Booklet

... Avian influenza is an infection caused by avian (bird) influenza (flu) viruses. Avian influenza is very contagious among birds and can make some domestic birds, including chickens, ducks and turkeys, very sick and kill them. Of the few avian influenza viruses that have crossed the species barrier to ...
Avian infectious bronchitis
Avian infectious bronchitis

... sequencing of the gene coding for the spike (S) protein or, more specifically, nucleotide sequencing of the gene coding for the S1 subunit of the S protein (Cavanagh, 1991; Kusters et al., 1989) where most of the epitopes to which neutralising antibodies bind are found (Koch et al., 1992). An exact ...
2 Viral Multiplication (Life Cycles)
2 Viral Multiplication (Life Cycles)

... bacteria growing in a petri plate. The bacteria have been infected with a bacteriophage- the ‘clearings’ (blank spaces called plaques) on the plate depict areas where a phage has infected and killed the bacteria. ...
Print this PDF
Print this PDF

... have a cough, feel very tired, and may have a sore throat as well. It can make you feel sick for a few days or for as long as a week. Sometimes, it can be hard to tell if you have the flu or a cold, but with the flu, you'll usually have a higher fever and feel much worse. The flu is a type of virus, ...
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Influenza A virus



Influenza A virus causes influenza in birds and some mammals, and is the only species of influenza virus A. Influenza virus A is a genus of the Orthomyxoviridae family of viruses. Strains of all subtypes of influenza A virus have been isolated from wild birds, although disease is uncommon. Some isolates of influenza A virus cause severe disease both in domestic poultry and, rarely, in humans. Occasionally, viruses are transmitted from wild aquatic birds to domestic poultry, and this may cause an outbreak or give rise to human influenza pandemics.Influenza A viruses are negative-sense, single-stranded, segmented RNA viruses.The several subtypes are labeled according to an H number (for the type of hemagglutinin) and an N number (for the type of neuraminidase). There are 18 different known H antigens (H1 to H18) and 11 different known N antigens (N1 to N11). H17 was isolated from fruit bats in 2012. H18N11 was discovered in a Peruvian bat in 2013.Each virus subtype has mutated into a variety of strains with differing pathogenic profiles; some are pathogenic to one species but not others, some are pathogenic to multiple species.A filtered and purified influenza A vaccine for humans has been developed, and many countries have stockpiled it to allow a quick administration to the population in the event of an avian influenza pandemic. Avian influenza is sometimes called avian flu, and colloquially, bird flu. In 2011, researchers reported the discovery of an antibody effective against all types of the influenza A virus.
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