Penaeus monodon by RT-PCR amplification
... electrophoresis detection of PCR products. These include possible modifications for rapid (30 min) membrane-based or microtiter plate-based visual detection by reverse hybridization using biotinylated dUTP incorporation during PCR amplification (see BoehringerMannheim catalogue). There is also a rec ...
... electrophoresis detection of PCR products. These include possible modifications for rapid (30 min) membrane-based or microtiter plate-based visual detection by reverse hybridization using biotinylated dUTP incorporation during PCR amplification (see BoehringerMannheim catalogue). There is also a rec ...
Diseases of Feral Swine Brochure
... reported between humans and swine. Humans can become infected by inhaling the virus or by touching surfaces contaminated with the virus and then touching the mouth or nose. In adults, influenza A viruses cause fever, chills, weakness, lethargy, coughing, and sneezing. The virus may cause diarrhea an ...
... reported between humans and swine. Humans can become infected by inhaling the virus or by touching surfaces contaminated with the virus and then touching the mouth or nose. In adults, influenza A viruses cause fever, chills, weakness, lethargy, coughing, and sneezing. The virus may cause diarrhea an ...
A Snotty Situation - Boone and Crockett Club
... lambs for several years. The local wildlife managers and biologist had also noticed she had a snotty nose and suspected pneumonia. The carcass came to me for an autopsy to investigate the cause of illness. When I examined her, I was surprised to find her lungs in pretty good shape for an old bighorn ...
... lambs for several years. The local wildlife managers and biologist had also noticed she had a snotty nose and suspected pneumonia. The carcass came to me for an autopsy to investigate the cause of illness. When I examined her, I was surprised to find her lungs in pretty good shape for an old bighorn ...
Ebola: history, treatment, and lessons from a new - AJP-Lung
... known. The five members of the Ebolavirus genus are the most highly studied of the Filoviridae, and the natural history of viral replication of filoviruses is largely known from studies of the Ebolavirus genus (Fig. 1). Filoviruses are enveloped RNA viruses that attach to host cells through glycopro ...
... known. The five members of the Ebolavirus genus are the most highly studied of the Filoviridae, and the natural history of viral replication of filoviruses is largely known from studies of the Ebolavirus genus (Fig. 1). Filoviruses are enveloped RNA viruses that attach to host cells through glycopro ...
Recent developments linking retroviruses to human breast cancer
... including those that target retroviruses, suggesting links between mutagenesis, immunity and viral infection in the process of cancer development (Henderson et al., 2014). Many viruses have evolved mechanisms to counteract APOBEC effects, and thus drugs enhancing APOBEC activity are being developed. ...
... including those that target retroviruses, suggesting links between mutagenesis, immunity and viral infection in the process of cancer development (Henderson et al., 2014). Many viruses have evolved mechanisms to counteract APOBEC effects, and thus drugs enhancing APOBEC activity are being developed. ...
42. Improved FMD diagnosis using real-time RT-PCR
... from the present study that these procedures are deficient for certain specimens. The results for the overseas samples show that while all VI/ELISA positive FMDV samples were also positive by real-time RT-PCR (100% sensitivity at a CT cut-off value of <39) (Table III), FMD viral genome was detected ...
... from the present study that these procedures are deficient for certain specimens. The results for the overseas samples show that while all VI/ELISA positive FMDV samples were also positive by real-time RT-PCR (100% sensitivity at a CT cut-off value of <39) (Table III), FMD viral genome was detected ...
34. Sampling, shipping, organisation and testing of inactivated samples to take advantage of molecular analysis Methods
... Eid-ul-Azha. Animals that are not sold for sacrifice may, after having been mixed with many other animals from different places, then go back to where they came from or perhaps be sold and go somewhere else. The majority of the commercial farmers in LCC vaccinate their animals, either with a good qu ...
... Eid-ul-Azha. Animals that are not sold for sacrifice may, after having been mixed with many other animals from different places, then go back to where they came from or perhaps be sold and go somewhere else. The majority of the commercial farmers in LCC vaccinate their animals, either with a good qu ...
Full Text - Harvard University
... methylated and not transcribed. In addition, the endogenous retroviral DNA sequence is usually not capable of encoding proteins due to numerous stop codons and frameshifts within ORFs. [For review (142, 169)] In addition, some of these retroelements can transpose intracellularly. Such a transpositio ...
... methylated and not transcribed. In addition, the endogenous retroviral DNA sequence is usually not capable of encoding proteins due to numerous stop codons and frameshifts within ORFs. [For review (142, 169)] In addition, some of these retroelements can transpose intracellularly. Such a transpositio ...
Ordinance No 4 dated 14.10.2002 on protection of workers from
... Article 1. This Ordinance determines the minimum requirements for protection of workers from existing or potential health and safety risks in the event of exposure to biological agents at the workplace. Article 2. This Ordinance is subject to application in all enterprises, places/locations, and act ...
... Article 1. This Ordinance determines the minimum requirements for protection of workers from existing or potential health and safety risks in the event of exposure to biological agents at the workplace. Article 2. This Ordinance is subject to application in all enterprises, places/locations, and act ...
Transmission of Cucumber Mosaic Virus (CMV)
... banana chlorosis was identified based on mechanical transmission, insect vector transmission, host range, serology DACELISA detection. The virus was able to transmit mechanically on the plants used in the study very efficiently and produced the systemic and local symptoms characteristic of CMV. In o ...
... banana chlorosis was identified based on mechanical transmission, insect vector transmission, host range, serology DACELISA detection. The virus was able to transmit mechanically on the plants used in the study very efficiently and produced the systemic and local symptoms characteristic of CMV. In o ...
FACIAL HERPES
... Facial herpes is spread by close physical contact between an infected person and somebody who was previously uninfected. Infection is most commonly acquired during infancy or childhood as a result of contact with relatives (for example kissing or hugging). The source does not always have typical fac ...
... Facial herpes is spread by close physical contact between an infected person and somebody who was previously uninfected. Infection is most commonly acquired during infancy or childhood as a result of contact with relatives (for example kissing or hugging). The source does not always have typical fac ...
virus - Birdi
... The disease (and also measles) was transferred to America by the Spanish, when they conquered the New World in the early 1500. 3.5 million Aztecs died in the next 2 years. In the cities of 18th century Europe, smallpox reached plague proportions and was a feared scourge - highly infectious. Five rei ...
... The disease (and also measles) was transferred to America by the Spanish, when they conquered the New World in the early 1500. 3.5 million Aztecs died in the next 2 years. In the cities of 18th century Europe, smallpox reached plague proportions and was a feared scourge - highly infectious. Five rei ...
Risk assessment on the importation of milk and milk products
... heated at 93°C for 15 sec. F M D V survived in whole milk which was heated at 72°C for 5 min and then evaporated. The virus survived in skimmed milk after this was heated at 72°C for 15 sec, but not when it was heated at 72°C for 30 sec and then evaporated. Most of the h e a t e d samples were found ...
... heated at 93°C for 15 sec. F M D V survived in whole milk which was heated at 72°C for 5 min and then evaporated. The virus survived in skimmed milk after this was heated at 72°C for 15 sec, but not when it was heated at 72°C for 30 sec and then evaporated. Most of the h e a t e d samples were found ...
TP219 acts as a selective inhibitor of in vitro enterovirus replication
... polyprotein synthesis/processing or (iii) the formation of replication vesicles. In addition no infectious virus particles were detected in TP219-treated infected cells, suggesting that the drug interferes with the formation of novel infectious viral progeny. TP219-resistant (TP219res) CVB3 was sele ...
... polyprotein synthesis/processing or (iii) the formation of replication vesicles. In addition no infectious virus particles were detected in TP219-treated infected cells, suggesting that the drug interferes with the formation of novel infectious viral progeny. TP219-resistant (TP219res) CVB3 was sele ...
Lentiviruses
... First, MAS does not involve drug use of any kind. This can be appealing on its own, and it avoids the issue of pathogens developing resistance to drugs that might be used in other species, such as humans. Further, genetic marker use scales well with flock size so that even large flocks can undergo M ...
... First, MAS does not involve drug use of any kind. This can be appealing on its own, and it avoids the issue of pathogens developing resistance to drugs that might be used in other species, such as humans. Further, genetic marker use scales well with flock size so that even large flocks can undergo M ...
Hepatitis B
... • Baruch Blumberg, 1963: ‘Australian antigen - Au'. • 1967: Au was a viral antigen = HBsAg (surface antigen) • Dane, 1970: Discovered 42nm 'Dane particles‘ HBcAg (core antigen). • 1973: HBeAg discovered (endogenous antigen = a truncated version of HBcAg). ...
... • Baruch Blumberg, 1963: ‘Australian antigen - Au'. • 1967: Au was a viral antigen = HBsAg (surface antigen) • Dane, 1970: Discovered 42nm 'Dane particles‘ HBcAg (core antigen). • 1973: HBeAg discovered (endogenous antigen = a truncated version of HBcAg). ...
Abstract
... Logit transformations of individual serum titration curves were used to titre sera and to compare titration curves generated by each antigen. Significant differences (P<0.01) were found between slopes of titration curves generated by each antigen preparation, however end point titres showed a high d ...
... Logit transformations of individual serum titration curves were used to titre sera and to compare titration curves generated by each antigen. Significant differences (P<0.01) were found between slopes of titration curves generated by each antigen preparation, however end point titres showed a high d ...
Evaluation of assay methods and false positive results in the
... In the mid-1970s, a new disease entity termed ‘’non-A, nonB’’ (NANB) hepatitis was first described, and in the following years leading to the discovery of the virus, posttransfusion and community-acquired NANB hepatitis increasingly became a potentially serious disease, resulting in cirrhosis and/or ...
... In the mid-1970s, a new disease entity termed ‘’non-A, nonB’’ (NANB) hepatitis was first described, and in the following years leading to the discovery of the virus, posttransfusion and community-acquired NANB hepatitis increasingly became a potentially serious disease, resulting in cirrhosis and/or ...
DETECTION OF INFECTIOUS BRONCHITIS VIRUS
... most important. Time elapsed between onset of infection and sampling The upper respiratory tract is the primary site of IBV replication, leading to viraemia and dissemination of the virus to other tissues (McMartin, 1993; Dhinakar Raj & Jones, 1997). All IBV strains can be isolated from the respirat ...
... most important. Time elapsed between onset of infection and sampling The upper respiratory tract is the primary site of IBV replication, leading to viraemia and dissemination of the virus to other tissues (McMartin, 1993; Dhinakar Raj & Jones, 1997). All IBV strains can be isolated from the respirat ...
Ebola virus-contaminated liquid waste
... deionized water (R2 > 0.91), but a fit better than that previously observed for Ebola virus in human blood (R2 < 0.29). The inactivation constant (k) was determined to be −1.08 when including the time zero time point and −0.35 when excluding the time zero time point. On the basis of the model fit, the ...
... deionized water (R2 > 0.91), but a fit better than that previously observed for Ebola virus in human blood (R2 < 0.29). The inactivation constant (k) was determined to be −1.08 when including the time zero time point and −0.35 when excluding the time zero time point. On the basis of the model fit, the ...
Myxoma virus in rabbits
... As in Australia, the initial epidemics of myxomatosis in France and Great Britain were highly lethal and rabbit numbers were greatly reduced. As shown above, the Lausanne strain of myxoma virus released in France and subsequently in Great Britain is inherently more virulent than the SLS released in ...
... As in Australia, the initial epidemics of myxomatosis in France and Great Britain were highly lethal and rabbit numbers were greatly reduced. As shown above, the Lausanne strain of myxoma virus released in France and subsequently in Great Britain is inherently more virulent than the SLS released in ...
Mutational analysis of the glycosaminoglycan
... wasting syndrome (PMWS) as the most significant manifestation (Segalés, 2012). ...
... wasting syndrome (PMWS) as the most significant manifestation (Segalés, 2012). ...
Feline Infectious Peritonitis Virus
... Our lab has developed a quantitative PCR targeting subgenomic mRNA of the M gene of Feline Coronavirus with high sensitivity. Thus, our PCR specifically detects and quantifies the replicating virus as opposed to only detecting the presence of viral genomic RNA that may or may not be associated with ...
... Our lab has developed a quantitative PCR targeting subgenomic mRNA of the M gene of Feline Coronavirus with high sensitivity. Thus, our PCR specifically detects and quantifies the replicating virus as opposed to only detecting the presence of viral genomic RNA that may or may not be associated with ...
Application APP202377 - Environmental Protection Authority
... Section 38I of the HSNO Act provides for a rapid assessment of applications seeking the release of qualifying organisms, where a qualifying organism is a new organism that is or is contained in a veterinary medicine1. This Staff Assessment Report considers the application against the criterion set o ...
... Section 38I of the HSNO Act provides for a rapid assessment of applications seeking the release of qualifying organisms, where a qualifying organism is a new organism that is or is contained in a veterinary medicine1. This Staff Assessment Report considers the application against the criterion set o ...
Norovirus and Histo-Blood Group Antigens
... “Recovirus,” was recently proposed (19) (Table 1). NoV strains have been isolated not only in humans but also in pigs, cattle, and mice, and they are classified into the type species Norwalk virus of the genus Norovirus, thus forming a group of small RNA viruses. NoV is a small, round, non-enveloped ...
... “Recovirus,” was recently proposed (19) (Table 1). NoV strains have been isolated not only in humans but also in pigs, cattle, and mice, and they are classified into the type species Norwalk virus of the genus Norovirus, thus forming a group of small RNA viruses. NoV is a small, round, non-enveloped ...
Influenza A virus
Influenza A virus causes influenza in birds and some mammals, and is the only species of influenza virus A. Influenza virus A is a genus of the Orthomyxoviridae family of viruses. Strains of all subtypes of influenza A virus have been isolated from wild birds, although disease is uncommon. Some isolates of influenza A virus cause severe disease both in domestic poultry and, rarely, in humans. Occasionally, viruses are transmitted from wild aquatic birds to domestic poultry, and this may cause an outbreak or give rise to human influenza pandemics.Influenza A viruses are negative-sense, single-stranded, segmented RNA viruses.The several subtypes are labeled according to an H number (for the type of hemagglutinin) and an N number (for the type of neuraminidase). There are 18 different known H antigens (H1 to H18) and 11 different known N antigens (N1 to N11). H17 was isolated from fruit bats in 2012. H18N11 was discovered in a Peruvian bat in 2013.Each virus subtype has mutated into a variety of strains with differing pathogenic profiles; some are pathogenic to one species but not others, some are pathogenic to multiple species.A filtered and purified influenza A vaccine for humans has been developed, and many countries have stockpiled it to allow a quick administration to the population in the event of an avian influenza pandemic. Avian influenza is sometimes called avian flu, and colloquially, bird flu. In 2011, researchers reported the discovery of an antibody effective against all types of the influenza A virus.