Making a firm decision: multifaceted regulation of cell fate in the
... development. We discuss how these models might be compatible with each other, and suggest that multiple mechanisms could work in concert. The core theme of this Review is how cells first gain and then main‑ tain their identity in the mouse embryo. We start by looking at zygotic genome activation and ...
... development. We discuss how these models might be compatible with each other, and suggest that multiple mechanisms could work in concert. The core theme of this Review is how cells first gain and then main‑ tain their identity in the mouse embryo. We start by looking at zygotic genome activation and ...
PDF
... (Fig. 3A-C). The movement of N lineage cells relative to lateral M lineage derivatives is revealed by a computer-generated sagittal view of the same region of the germinal plate (Fig. 3D). Similar experiments were carried out to examine cell neighbor relations between the N lineage and the lateral a ...
... (Fig. 3A-C). The movement of N lineage cells relative to lateral M lineage derivatives is revealed by a computer-generated sagittal view of the same region of the germinal plate (Fig. 3D). Similar experiments were carried out to examine cell neighbor relations between the N lineage and the lateral a ...
Genetic Regulation of Embryonic Pattern Formation
... 2001). Therefore, imprinting is unlikely to be a general mechanism; more likely, it is a specific mechanism that affects the expression of some but not all genes during plant embryo development. What is the significance of imprinting? Analysis of imprinted loci as well as reciprocal crosses between ...
... 2001). Therefore, imprinting is unlikely to be a general mechanism; more likely, it is a specific mechanism that affects the expression of some but not all genes during plant embryo development. What is the significance of imprinting? Analysis of imprinted loci as well as reciprocal crosses between ...
Phosphorylation-Dependent Regulation of Septin
... regulation of septin dynamics, possibly through the control of their phosphorylation state, is required for the completion of cytokinesis. Introduction In order to give rise to two viable progenies, cytokinesis must be both spatially and temporally coordinated with the partition of sister chromatids ...
... regulation of septin dynamics, possibly through the control of their phosphorylation state, is required for the completion of cytokinesis. Introduction In order to give rise to two viable progenies, cytokinesis must be both spatially and temporally coordinated with the partition of sister chromatids ...
comparison of p53 expression in hpv(+)
... All samples were taken from the paraffin block of squamous cell neoplasia that arouse on the eyelid and ocular surface that were operated between 2000-2007 in Sardjito General Hospital and Yap Eye Hospital. All samples were deparaffinized and cut in slices. Then it was stained using DO-7 humanized m ...
... All samples were taken from the paraffin block of squamous cell neoplasia that arouse on the eyelid and ocular surface that were operated between 2000-2007 in Sardjito General Hospital and Yap Eye Hospital. All samples were deparaffinized and cut in slices. Then it was stained using DO-7 humanized m ...
Lesson (1) Cell theory
... the eyepiece and objective lenses in it. They cannot magnify more than 1500 times because this make the photo unclear. The magnification power of light microscope = Magnification power of eyepiece x magnification power of objective lense Scientists discovered better ways to observe samples clearly b ...
... the eyepiece and objective lenses in it. They cannot magnify more than 1500 times because this make the photo unclear. The magnification power of light microscope = Magnification power of eyepiece x magnification power of objective lense Scientists discovered better ways to observe samples clearly b ...
Simple microwave field imaging technique using hot atomic vapor
... vapor cell with p = 10 mbar of Ne buffer gas at a temperature of T ≈ 115◦C. After optical pumping with a laser intensity of I0 = 0.2 mW/cm2 , we measure an optical density OD ≈ 0.5. The microwave pulse is applied with Pmw ≈ 5 W and dtmw is varied between 1 and 30 µs. Figure 2a (bottom) shows the obs ...
... vapor cell with p = 10 mbar of Ne buffer gas at a temperature of T ≈ 115◦C. After optical pumping with a laser intensity of I0 = 0.2 mW/cm2 , we measure an optical density OD ≈ 0.5. The microwave pulse is applied with Pmw ≈ 5 W and dtmw is varied between 1 and 30 µs. Figure 2a (bottom) shows the obs ...
Splenic Epstein-Barr Virus–Associated Inflammatory Pseudotumor
... from the uninvolved splenic parenchyma (left) by a thin fibrous capsule (arrowheads). C, A dense lymphoplasmacytic infiltrate obscures the spindle cell component. The inflammatory infiltrate shows some multinucleated histiocytes (long arrows) and plasma cells containing Russell bodies (short arrows) ...
... from the uninvolved splenic parenchyma (left) by a thin fibrous capsule (arrowheads). C, A dense lymphoplasmacytic infiltrate obscures the spindle cell component. The inflammatory infiltrate shows some multinucleated histiocytes (long arrows) and plasma cells containing Russell bodies (short arrows) ...
Munc18b is an essential gene in mice whose expression is limiting
... of the exocytic and endocytic pathways of eukaryotic cells [1–5]. SNARE proteins form a four-helix bundle that is responsible for the specific pairing of donor and target membranes and for inducing membrane fusion. SM proteins provide a scaffold for the assembly of SNARE bundles, and in regulated ex ...
... of the exocytic and endocytic pathways of eukaryotic cells [1–5]. SNARE proteins form a four-helix bundle that is responsible for the specific pairing of donor and target membranes and for inducing membrane fusion. SM proteins provide a scaffold for the assembly of SNARE bundles, and in regulated ex ...
Full Text - Plant and Cell Physiology
... transmission electron microscopy (TEM) have shown that the localization of CESAs is not restricted to the PM; CESAs are also detected in several intracellular compartments including the Golgi apparatus, the trans-Golgi network (TGN) and the distinctive organelles referred to as microtubule-associate ...
... transmission electron microscopy (TEM) have shown that the localization of CESAs is not restricted to the PM; CESAs are also detected in several intracellular compartments including the Golgi apparatus, the trans-Golgi network (TGN) and the distinctive organelles referred to as microtubule-associate ...
Myosin X is a downstream effector of PI(3)K during phagocytosis articles
... PH domains of Myo10 inhibited phagocytosis (Fig. 4b). In contrast, expression of GFP-tagged full-length Myo10 (Myo10 WT), or untagged full-length Myo10 (Myo10 WT*), did not result in a significant inhibition of phagocytosis (Fig. 4b). The lack of an effect on phagocytosis by some of the constructs w ...
... PH domains of Myo10 inhibited phagocytosis (Fig. 4b). In contrast, expression of GFP-tagged full-length Myo10 (Myo10 WT), or untagged full-length Myo10 (Myo10 WT*), did not result in a significant inhibition of phagocytosis (Fig. 4b). The lack of an effect on phagocytosis by some of the constructs w ...
Kwak, 2005 - U of L Class Index
... SCM regulates the expression of the GLABRA2, CAPRICE, WEREWOLF, and ENHANCER OF GLABRA3 transcription factor genes that define the cell fates. Further, the SCM gene is expressed throughout the developing root. Therefore, SCM likely enables developing epidermal cells to detect positional cues and est ...
... SCM regulates the expression of the GLABRA2, CAPRICE, WEREWOLF, and ENHANCER OF GLABRA3 transcription factor genes that define the cell fates. Further, the SCM gene is expressed throughout the developing root. Therefore, SCM likely enables developing epidermal cells to detect positional cues and est ...
Truncated N-glycans affect protein folding in the ER of CHO
... previously showed that this reduction in Mw is caused by trimming of the glycans (Ermonval et al., 1997). The processing of N-glycans starts in the ER with the removal of the three glucose residues by glucosidases I and II, and subsequent cleavage of one mannose residue by the ER-mannosidase I speci ...
... previously showed that this reduction in Mw is caused by trimming of the glycans (Ermonval et al., 1997). The processing of N-glycans starts in the ER with the removal of the three glucose residues by glucosidases I and II, and subsequent cleavage of one mannose residue by the ER-mannosidase I speci ...
Chloroplasts – Structure and Function
... dissolved salts, ribosomes and a circular strand of DNA. It is in the stroma that the light-independent reactions take place. ...
... dissolved salts, ribosomes and a circular strand of DNA. It is in the stroma that the light-independent reactions take place. ...
NuMA assembles into an extensive filamentous structure when
... localized in the cell nucleus to sequester it away from the cytoplasmic microtubules we decided to examine how cells progress through mitosis if NuMA is present in the cell cytoplasm instead of the nucleus. The amino acid sequence necessary and sufficient for NuMA’s localization in the nucleus has b ...
... localized in the cell nucleus to sequester it away from the cytoplasmic microtubules we decided to examine how cells progress through mitosis if NuMA is present in the cell cytoplasm instead of the nucleus. The amino acid sequence necessary and sufficient for NuMA’s localization in the nucleus has b ...
Lab 4
... • Learn methods of differential staining: – Use two or more stains and categorize cells into groups – Gram Staining – Separates bacteria in two different groups – Gram positive and Gram negative – Important first test for bacterial identification ...
... • Learn methods of differential staining: – Use two or more stains and categorize cells into groups – Gram Staining – Separates bacteria in two different groups – Gram positive and Gram negative – Important first test for bacterial identification ...
Cell Division chapt09
... A new cell wall is built, separating the nuclei. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. ...
... A new cell wall is built, separating the nuclei. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. ...
Weekly Lesson Plans Teacher: Alicia Penfield Class: AP Biology
... The students will… The students will… Explain metabolism and the Explain why photosynthesis role of matter and energy in is an anabolic reaction, and this process. its overall purpose. Distinguish exergonic and Summarize each stage of endergonic reactions, and photosynthesis, including the g ...
... The students will… The students will… Explain metabolism and the Explain why photosynthesis role of matter and energy in is an anabolic reaction, and this process. its overall purpose. Distinguish exergonic and Summarize each stage of endergonic reactions, and photosynthesis, including the g ...
Andrei Fagarasanu Department of Cell Biology Supervisor: Dr
... distant organisms led to the discovery of several patches of conserved residues in both subdomains I and II (Pashkova et al., 2006). Conserved surface residues generally indicate sites of protein–protein interaction. Therefore, the conserved patches on the surface of the Myo2p cargo-binding domain a ...
... distant organisms led to the discovery of several patches of conserved residues in both subdomains I and II (Pashkova et al., 2006). Conserved surface residues generally indicate sites of protein–protein interaction. Therefore, the conserved patches on the surface of the Myo2p cargo-binding domain a ...
Outer dense fibre protein 2 (ODF2) is a self
... Outer dense fibre protein 2 (ODF2) is a major protein of sperm tail outer dense fibres (Brohmann et al., 1997; Shao et al., 1997; Turner et al., 1997; Schalles et al., 1998). The outer dense fibres (ODF) are prominent sperm tail-specific cytoskeletal structures. They consist of nine fibres that acco ...
... Outer dense fibre protein 2 (ODF2) is a major protein of sperm tail outer dense fibres (Brohmann et al., 1997; Shao et al., 1997; Turner et al., 1997; Schalles et al., 1998). The outer dense fibres (ODF) are prominent sperm tail-specific cytoskeletal structures. They consist of nine fibres that acco ...
LL37 peptide@silver nanoparticles: combining control
... similar to AgNO3 and AgSD. In time kill experiments (Fig. 2), E. coli was considerably more susceptible to ionic silver, as previously reported in literature.[14] However, Fig. 2BD show that the bactericidal performance of LL37@AgNP was similar to that observed for AgNO3 against P. aeruginosa, S. ep ...
... similar to AgNO3 and AgSD. In time kill experiments (Fig. 2), E. coli was considerably more susceptible to ionic silver, as previously reported in literature.[14] However, Fig. 2BD show that the bactericidal performance of LL37@AgNP was similar to that observed for AgNO3 against P. aeruginosa, S. ep ...
Potential effect of spliceosome inhibition in small cell lung
... therapeutic vulnerability in MYC-driven breast cancers, we evaluated the efficacy of a spliceosome inhibitor in SCLC cell lines and analyzed the correlation with MYC status. Among 23 SCLC cell lines examined, eight showed high MYC protein expression (> 80% positive cells) by immunohistochemistry (IH ...
... therapeutic vulnerability in MYC-driven breast cancers, we evaluated the efficacy of a spliceosome inhibitor in SCLC cell lines and analyzed the correlation with MYC status. Among 23 SCLC cell lines examined, eight showed high MYC protein expression (> 80% positive cells) by immunohistochemistry (IH ...
Chapter 4
... Biology and Society: Drugs That Target Bacterial Cells – Antibiotics were first isolated from mold in 1928. – The widespread use of antibiotics drastically ...
... Biology and Society: Drugs That Target Bacterial Cells – Antibiotics were first isolated from mold in 1928. – The widespread use of antibiotics drastically ...
Microcavity Lasers for Cancer Cell Detection
... http://www.cancer.umn.edu/page/docs/fcintro.pdf NASA, Cancer Detection Device, SpinOff (1998) ...
... http://www.cancer.umn.edu/page/docs/fcintro.pdf NASA, Cancer Detection Device, SpinOff (1998) ...
Cytokinesis
Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.