Protein Purification
... Size estimation: mobility depends linearly on log MW, standard curve required ...
... Size estimation: mobility depends linearly on log MW, standard curve required ...
Labratory Examination Questions
... other enzymes it is useful to cool the sample on ice during this process. After homogenization, the first step of cell fractionation is carried out in order to isolate nuclear fraction and post nuclear supernatant. DNA is found in nucleus as a DNA-protein complex. The first extraction buffer used Na ...
... other enzymes it is useful to cool the sample on ice during this process. After homogenization, the first step of cell fractionation is carried out in order to isolate nuclear fraction and post nuclear supernatant. DNA is found in nucleus as a DNA-protein complex. The first extraction buffer used Na ...
Macromolecules: Building blocks of life
... Today’s notes: • We will be getting information from this power point and putting it into a “foldable”. • We will need four flaps one for each of the macromolecules we will be learning. • You will put the names of the 4 macromolecules on each of the flaps and put some important information about ea ...
... Today’s notes: • We will be getting information from this power point and putting it into a “foldable”. • We will need four flaps one for each of the macromolecules we will be learning. • You will put the names of the 4 macromolecules on each of the flaps and put some important information about ea ...
The basis of specific ligand recognition by proteins
... Experimental structure determination by X-ray crystallography or NMR has provided many examples of high-resolution structures of complexes between proteins and drugs, DNA or simply other proteins [1]. However, what really determines binding specificity is still largely unknown, as the very limited s ...
... Experimental structure determination by X-ray crystallography or NMR has provided many examples of high-resolution structures of complexes between proteins and drugs, DNA or simply other proteins [1]. However, what really determines binding specificity is still largely unknown, as the very limited s ...
DNA Restriction and Gel Electrophoresis This laboratory
... However, for DNA, the charge-mass ratio is almost always the same, so that migration speed ...
... However, for DNA, the charge-mass ratio is almost always the same, so that migration speed ...
Steps in a Western blot
... The proteins of the sample are separated using gel electrophoresis. Separation of proteins may be by isoelectric point (pI), molecular weight, electric charge, or a combination of these factors. The nature of the separation depends on the treatment of the sample and the nature of the gel. By far the ...
... The proteins of the sample are separated using gel electrophoresis. Separation of proteins may be by isoelectric point (pI), molecular weight, electric charge, or a combination of these factors. The nature of the separation depends on the treatment of the sample and the nature of the gel. By far the ...
Western Blotting
... The following blotting procedures are recommended for all NuSep Gels. It is recommended to read apparatus manual before conduction blots as varying blotting conditions will alter results. It is recommended that you use NuSep Transfer Buffer BG-168. The following recipe can also be used: • Tris Base ...
... The following blotting procedures are recommended for all NuSep Gels. It is recommended to read apparatus manual before conduction blots as varying blotting conditions will alter results. It is recommended that you use NuSep Transfer Buffer BG-168. The following recipe can also be used: • Tris Base ...
Total protein extracts prep
... Use 110 µl per gel lane and top up with sample buffer. If 2D electrophoresis is desired precipitate total protein with acetone as described in protocol and resuspend in IEF or NepHGE sample buffer. ...
... Use 110 µl per gel lane and top up with sample buffer. If 2D electrophoresis is desired precipitate total protein with acetone as described in protocol and resuspend in IEF or NepHGE sample buffer. ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.