tools of genetic engineering
... Agarose gel electrophoresis is a commonly used method for separating nucleic acid molecules by length using an electric field. Gel electrophoresis is a very common technique to separate DNA, RNA or proteins based on their size or charge. To perform electrophoresis, a gel, often made of agarose, is u ...
... Agarose gel electrophoresis is a commonly used method for separating nucleic acid molecules by length using an electric field. Gel electrophoresis is a very common technique to separate DNA, RNA or proteins based on their size or charge. To perform electrophoresis, a gel, often made of agarose, is u ...
S1 Fig.
... UltiMate® 3000 HPLC series (Dionex, Sunnyvale, CA USA) was used for peptide concentration and separation. Samples were separated in Nano Series™ Standard Columns (75 µm i.d. x 15 cm) packed with C18 PepMap100 (3 µm, 100Å). The gradient used was from 3.2% to 44% solvent B (0.1% formic acid in acetoni ...
... UltiMate® 3000 HPLC series (Dionex, Sunnyvale, CA USA) was used for peptide concentration and separation. Samples were separated in Nano Series™ Standard Columns (75 µm i.d. x 15 cm) packed with C18 PepMap100 (3 µm, 100Å). The gradient used was from 3.2% to 44% solvent B (0.1% formic acid in acetoni ...
Proteomics - OpenWetWare
... Relies heavily on data-base search engines and other bioinformatics tools to interpret/generate the data ...
... Relies heavily on data-base search engines and other bioinformatics tools to interpret/generate the data ...
DNA fingerprinting Genes and DNA
... DNA Fingerprinting - STR • Benefits – At least 13 loci are used which assort independently. • High degree of accuracy based on statistics • The probably of a particular combination of these 13 loci is one in a quintillion (1 with 18 zeros after it). • This means that it is statistically impossible f ...
... DNA Fingerprinting - STR • Benefits – At least 13 loci are used which assort independently. • High degree of accuracy based on statistics • The probably of a particular combination of these 13 loci is one in a quintillion (1 with 18 zeros after it). • This means that it is statistically impossible f ...
Manipulating DNA
... 2. Look carefully at the series, and find this sequence of letters: GTTAAC. It may appear more than once. How many occurrences of the sequence GTTAAC can you find? ...
... 2. Look carefully at the series, and find this sequence of letters: GTTAAC. It may appear more than once. How many occurrences of the sequence GTTAAC can you find? ...
GeneMATRIX PCR / DNA Clean-Up Purification Kit
... Note 1: Once the kit is unpacked, store components at room temperature. In case of occasional buffer ingredients precipitation, simply warm up in 37 oC water bath, until clarified. Note 2: All solutions should be kept tightly closed to avoid evaporation and resulting components concentration change ...
... Note 1: Once the kit is unpacked, store components at room temperature. In case of occasional buffer ingredients precipitation, simply warm up in 37 oC water bath, until clarified. Note 2: All solutions should be kept tightly closed to avoid evaporation and resulting components concentration change ...
DNA – Structure and Replication
... restriction fragments after the cutting stage The mixture of restriction fragments can be separated out into a unique pattern of bands The process of separating out the different fragments is carried out by gel electrophoresis ...
... restriction fragments after the cutting stage The mixture of restriction fragments can be separated out into a unique pattern of bands The process of separating out the different fragments is carried out by gel electrophoresis ...
EDVOTEK 225 DNA Fingerprinting
... between recognition sites. • Long DNA molecule will have higher probability recognition site will be present. • Restriction enzyme with 6 bp recognition site expected to cut human DNA into approximately 750,000 different fragments ...
... between recognition sites. • Long DNA molecule will have higher probability recognition site will be present. • Restriction enzyme with 6 bp recognition site expected to cut human DNA into approximately 750,000 different fragments ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.