Schematic courtesy of B. Crump Quantitative (Real Time) PCR
... 5. Wash filter-bound DNA several times with alcohol 6. Elute DNA off membrane with pH 8, low-salt buffer ...
... 5. Wash filter-bound DNA several times with alcohol 6. Elute DNA off membrane with pH 8, low-salt buffer ...
primary structure
... SDS PAGE Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) is a routinely applied analytical method to estimate the purity and molecular weight of proteins in a sample (Fig. 3-18). Proteins are dissolved in a buffer containing SDS and separated on a polyacrylamide gel matrix by t ...
... SDS PAGE Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) is a routinely applied analytical method to estimate the purity and molecular weight of proteins in a sample (Fig. 3-18). Proteins are dissolved in a buffer containing SDS and separated on a polyacrylamide gel matrix by t ...
Stress response of some lactic acid bacteria with
... differences in cytosolic protein patterns were analysed by one dimensional Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). The activity of some intracellular enzymes involved in different metabolic pathways (lactate dehydrogenase, alcohol dehydrogenase, superoxide dismutase, ma ...
... differences in cytosolic protein patterns were analysed by one dimensional Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). The activity of some intracellular enzymes involved in different metabolic pathways (lactate dehydrogenase, alcohol dehydrogenase, superoxide dismutase, ma ...
5. QIAquick® PCR Purification Kit
... The QIAquick PCR Purification Kit (cat. nos. 28104 and 28106) can be stored at room temperature (15–25°C) for up to 12 months. For more information, please refer to the QIAquick Spin Handbook, March 2008, which can be found at: www.qiagen.com/handbooks. Notes before starting ...
... The QIAquick PCR Purification Kit (cat. nos. 28104 and 28106) can be stored at room temperature (15–25°C) for up to 12 months. For more information, please refer to the QIAquick Spin Handbook, March 2008, which can be found at: www.qiagen.com/handbooks. Notes before starting ...
DNA
... • less commonly used due to the capacity of other techniques, such as PCR. • Southern blotting are still used for some applications such as measuring transgene copy number in transgenic mice, or in the engineering of gene knockout embryonic stem cell lines. ...
... • less commonly used due to the capacity of other techniques, such as PCR. • Southern blotting are still used for some applications such as measuring transgene copy number in transgenic mice, or in the engineering of gene knockout embryonic stem cell lines. ...
Techniques Used to Test Native DNA
... Speaking the Language of DNA This guide can help you better understand the techniques commonly used to test native high-molecular-weight DNA. Electrophoresis—A method for separating macromolecules on the basis of size and net electrical charge. Dot blot—A technique to determine whether a particular ...
... Speaking the Language of DNA This guide can help you better understand the techniques commonly used to test native high-molecular-weight DNA. Electrophoresis—A method for separating macromolecules on the basis of size and net electrical charge. Dot blot—A technique to determine whether a particular ...
Ion exchange chromatography File
... Resins with low degree of cross-linking are more permeable to high molecular weight compounds, but they are less rigid and swell more when placed in buffer Sulphonation of cross-linked polystyrene results in sulphonated polystyrene resin such as Dowex 50- strong acidic exchanger Basic exchangers are ...
... Resins with low degree of cross-linking are more permeable to high molecular weight compounds, but they are less rigid and swell more when placed in buffer Sulphonation of cross-linked polystyrene results in sulphonated polystyrene resin such as Dowex 50- strong acidic exchanger Basic exchangers are ...
Datasheet for Alkaline Phosphatase, Calf Intestinal (CIP)
... DNA, RNA and ribo- and deoxyribonucleoside triphosphates. Since CIP-treated fragments lack the 5´ phosphoryl termini required by ligases, they cannot self-ligate (1). This property can be used to decrease the vector background in cloning strategies. Source: Calf intestinal mucosa Molecular Weight: 6 ...
... DNA, RNA and ribo- and deoxyribonucleoside triphosphates. Since CIP-treated fragments lack the 5´ phosphoryl termini required by ligases, they cannot self-ligate (1). This property can be used to decrease the vector background in cloning strategies. Source: Calf intestinal mucosa Molecular Weight: 6 ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.